Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 4,435,089 T→C *287* (TAA→TAG)  qorB ← NAD(P)H:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252684,435,0890TC99.7% 1333.3 / NA 384*287* (TAA→TAG) qorBNAD(P)H:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (238/145);  minor base A (1/0);  total (239/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 398 total aligned reads displayed.

TAACAGGGCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATAT  >  NZ_CP025268/4434952‑4435225
                                                                                                                                         |                                                                                                                                        
    aGGGCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCTGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACGTTAATTAACTAGtta                                                                                                                                    >  2:585696/1‑140 (MQ=255)
    aGGGCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGTATGCGGGAGGATGCCACTTTAATTAACTAGtta                                                                                                                                    >  1:2043044/1‑140 (MQ=255)
    aGGGCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCGGTCAGAAGAAAGTTCAGGAAGCAGACCTGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGtta                                                                                                                                    >  1:3563570/1‑140 (MQ=255)
     gggCGTACGGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGttat                                                                                                                                   >  2:1001037/1‑140 (MQ=255)
     gggCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGttat                                                                                                                                   >  1:8921501/1‑140 (MQ=255)
     gggCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGttat                                                                                                                                   <  1:1776284/140‑1 (MQ=255)
      ggCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGttatt                                                                                                                                  >  1:9089610/1‑140 (MQ=255)
      ggCGAACATCACGCCGCTAAACATTACTGGCATCCATTGATCAGTCAGAAGACCGGTCAGGCCGCAGACATTATTGATCCCGGAATCCCGTCATTCTCAGAGAGGATGCGGGCGGATGCCACGTTAATTCACTAGtggtg                                                                                                                                  >  2:4464198/1‑136 (MQ=255)
       gCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGttatta                                                                                                                                 <  1:1124597/140‑1 (MQ=255)
        cGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTaa                                                                                                                                >  1:5665687/1‑140 (MQ=255)
         gTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAAc                                                                                                                               >  1:5298570/1‑140 (MQ=255)
         gTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAAc                                                                                                                               >  2:4694406/1‑140 (MQ=255)
         gTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAAc                                                                                                                               >  1:3796069/1‑140 (MQ=255)
         gTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAAc                                                                                                                               >  1:764441/1‑140 (MQ=255)
              gCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTaa                                                                                                                          >  1:8486478/1‑140 (MQ=255)
                aCGCCGCTAATCATTACTGGCATCCATTGATGTGTCAGAAGAAAGTTCAGGAAGCAGTCATGATTGCTCCCGGCATCCCGTCATTATCCGAGAGGATGCGGGAGGATGCCACTTTATTTAACTAGGTATTAACATTaaaa                                                                                                                        >  2:5520380/1‑140 (MQ=255)
                aCGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTaaaa                                                                                                                        >  2:3009528/1‑140 (MQ=255)
                 cgccgcTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTaaaaa                                                                                                                       >  2:2438821/1‑140 (MQ=255)
                 cgccgcTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTaaaaa                                                                                                                       >  1:6207707/1‑140 (MQ=255)
                 cgccgcTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTaaaaa                                                                                                                       >  1:4566536/1‑140 (MQ=255)
                 cgccgcTAATCAGTACTGGCATCCATTGATCTGTCAGAAGAAAGGTCAGGAAGCCGCCATGGTTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACGTTAATTAACTAGTTATTAACATTaaaat                                                                                                                       >  2:841801/1‑139 (MQ=255)
                  gccgcTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGTAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAg                                                                                                                      >  1:3263561/1‑140 (MQ=255)
                  gccgcTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAg                                                                                                                      >  2:11312/1‑140 (MQ=255)
                    cgcTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGAt                                                                                                                    >  2:425232/1‑140 (MQ=255)
                    cgcTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTGTAATTAACTAGTTATTCACATTAAAAAGAt                                                                                                                    >  2:7263467/1‑140 (MQ=255)
                         aTCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCtt                                                                                                               >  2:4632460/1‑140 (MQ=255)
                              tACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCt                                                                                                          >  1:547677/1‑140 (MQ=255)
                              tACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCt                                                                                                          >  1:9427781/1‑140 (MQ=255)
                              gaCTCGCATCCATTGATCTGCCAGAAGCAAGTTCAGGAAGCAGACACGATTGCTCCCGGCAGCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTCATTACCTAGTTATTAACCGTCAAAAGATGGCTTACGCt                                                                                                          <  2:1796876/139‑1 (MQ=255)
                               aCTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCtt                                                                                                         <  1:2484883/140‑1 (MQ=255)
                               aCTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCtt                                                                                                         <  1:4987316/140‑1 (MQ=255)
                                cTGGCATCCATTGATCTGTCAGATGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCtgt                                                                                                        >  2:7091699/1‑138 (MQ=255)
                                   gCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCgg                                                                                                     >  1:1898000/1‑140 (MQ=255)
                                    cATCCATTGATCCGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGc                                                                                                    <  1:6940409/140‑1 (MQ=255)
                                     aTCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCt                                                                                                   <  1:1295190/140‑1 (MQ=255)
                                     aTCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCt                                                                                                   >  1:5822343/1‑140 (MQ=255)
                                     aTCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACAGTAAAAAGATGGCTTACGCTTTCGGCt                                                                                                   >  2:2852083/1‑140 (MQ=255)
                                     aTCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCACGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCt                                                                                                   >  1:5569007/1‑140 (MQ=255)
                                      tCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTa                                                                                                  >  1:9213818/1‑140 (MQ=255)
                                      tCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTa                                                                                                  >  1:8682044/1‑140 (MQ=255)
                                      tCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTa                                                                                                  >  1:1648279/1‑140 (MQ=255)
                                      tCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTa                                                                                                  >  2:3215246/1‑140 (MQ=255)
                                        cATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAAc                                                                                                >  1:6022109/1‑140 (MQ=255)
                                        cATAGATCAGACAGAAGAAAGAACACCAAGCAGACAACAAAGCTCCCGGCATCCCGACATAATCACAGAGCATCCGGGACTATGCCACTATAATTAACTAGGTATTAACATTAAAAAGATGGCTTACGCAATCTGCTAAc                                                                                                >  2:3338836/1‑140 (MQ=255)
                                          ttGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTCGTTATTAACATTAAAAAGAGGGCTTACGCTTTCGGCTAACGt                                                                                              <  1:7477449/140‑1 (MQ=255)
                                          ttGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGt                                                                                              <  1:6974038/140‑1 (MQ=255)
                                          ttGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGt                                                                                              <  1:4117372/140‑1 (MQ=255)
                                             ctCTGTCAGAAGTAACTTCTGGAAGCAGACATGATTGCGCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTCGGTATTAACCTTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGt                                                                                           <  2:6846367/139‑1 (MQ=255)
                                              tCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTc                                                                                          <  1:1025617/140‑1 (MQ=255)
                                                  tCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTg                                                                                      >  2:1452226/1‑140 (MQ=255)
                                                  tCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTg                                                                                      >  1:4194782/1‑140 (MQ=255)
                                                  tCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTg                                                                                      >  2:6106641/1‑140 (MQ=255)
                                                  tCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACGAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTg                                                                                      >  2:2364450/1‑140 (MQ=255)
                                                  tCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATAAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTg                                                                                      >  2:2237926/1‑140 (MQ=255)
                                                    agaagaAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTggg                                                                                    <  1:2248304/140‑1 (MQ=255)
                                                         aaaGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGcc                                                                               >  1:8373708/1‑140 (MQ=255)
                                                            gTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTCACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCcaat                                                                            >  2:7436023/1‑140 (MQ=255)
                                                                     gCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCt                                                                   <  1:1687349/140‑1 (MQ=255)
                                                                     gCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCt                                                                   <  2:2656164/140‑1 (MQ=255)
                                                                     gCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCt                                                                   <  2:441218/140‑1 (MQ=255)
                                                                       aGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTaa                                                                 >  1:1479078/1‑140 (MQ=255)
                                                                            tGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGtt                                                            >  1:269761/1‑140 (MQ=255)
                                                                                tGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGc                                                        <  1:9155775/140‑1 (MQ=255)
                                                                                tGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGc                                                        <  1:3104084/140‑1 (MQ=255)
                                                                                tGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGc                                                        <  1:7742912/140‑1 (MQ=255)
                                                                                tGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGc                                                        <  1:7291338/140‑1 (MQ=255)
                                                                                tGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGc                                                        <  1:6454399/140‑1 (MQ=255)
                                                                                  cTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTg                                                      <  1:2755491/140‑1 (MQ=255)
                                                                                  cTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTg                                                      >  1:679907/1‑140 (MQ=255)
                                                                                    cccGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGtc                                                    <  2:8990343/140‑1 (MQ=255)
                                                                                       ggCATCCCGTCGTTATCAGAGAGGATGCGGGAGGATTCCACTTTAATTAACTAGGAATTAACATTAAAATGATGGCTTACGCTTGCGGCTAACGTTGTCGTTGGGTGGCCTGTCAATTTGGGATGTGTTTTGCTGtcatc                                                 >  2:8884247/1‑140 (MQ=255)
                                                                                       ggCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGtcatc                                                 >  1:8079903/1‑140 (MQ=255)
                                                                                        gCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGtcatca                                                >  1:7577904/1‑140 (MQ=255)
                                                                                          aTCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCaaa                                              <  2:7549879/140‑1 (MQ=255)
                                                                                            cccGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACa                                            >  1:6622256/1‑140 (MQ=255)
                                                                                              cGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGa                                          <  2:6484095/140‑1 (MQ=255)
                                                                                               gTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGAc                                         <  1:2291256/140‑1 (MQ=255)
                                                                                                       cAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTc                                 >  2:8808552/1‑140 (MQ=255)
                                                                                                          agtggATGCGTGAGGATTCCACTTTAATTAACTAGTTAAGAACATTACAAAGATCGCTTACGCTTTCGGCTAACGTTGTCGTTGTGTGGCCAATCAATTTGATAATTGTAATGCTGTCAACAAACAGACCTCCTTTCGAt                              <  2:3953882/137‑1 (MQ=255)
                                                                                                          agagGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGAt                              >  2:8260569/1‑140 (MQ=255)
                                                                                                          agagGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGAt                              >  2:7359468/1‑140 (MQ=255)
                                                                                                              gATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATgcgc                          >  1:4819740/1‑140 (MQ=255)
                                                                                                               aTGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGcc                         >  1:2927984/1‑140 (MQ=255)
                                                                                                                tGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCa                        >  2:2010892/1‑140 (MQ=255)
                                                                                                                tGCGGGAGGATGCCACATTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCa                        <  2:4809054/140‑1 (MQ=255)
                                                                                                                    ggaggaTGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTGGGGTGGCCAATCACTTTGCTCAGTGTTTTGCTGTCATCAAACAGACCGCCGTTCGATGCGCCACCGt                    >  2:9310719/1‑140 (MQ=255)
                                                                                                                      aggaTGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCa                  >  1:591848/1‑140 (MQ=255)
                                                                                                                        gaTGCCACTTTAATTAACTAGTTATTAACCTTAAAAAGATGGCTTACGCTTTCGGCTATCGTTGTAGTTGGGTGGCCAATCAATTTGCTAAGTTTTTTTCTGTCATCAAACAGACAGCCTTTCGATGCGCCAACGTCAGa                <  2:3773522/140‑1 (MQ=255)
                                                                                                                        gaTGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGTTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGa                >  1:4340629/1‑140 (MQ=255)
                                                                                                                         aTGCCACTTTAATTCACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGaa               >  2:533307/1‑140 (MQ=255)
                                                                                                                         aTGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGaa               >  2:6360804/1‑140 (MQ=255)
                                                                                                                          tGCCCCTTTAATTAACTAGTGAGGAACAGTAACACGATGGCTTACGCTGTCGGCTCACGTTGTCGTTGGGTGGCCCATCAAGTTGCTAAGTGGGTTGCTGTGATCAATCAGACCGCCGTTCGATGCGCCCACGTCCGact              >  2:1648141/1‑138 (MQ=255)
                                                                                                                          tGCCACTTTATTTATCTAGTTAGTAACATTAACGAGATGGCTTACGCTTTCGGCTCACGTGGTCGTTGGGTGGCGAATCATTTTGCGAAGTGTTTTGCTGTCATCATTCAGACCGCCGTTCGAGGCGCCCACGTCTGAAt              >  2:783281/1‑140 (MQ=255)
                                                                                                                          tGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAAt              >  1:3678569/1‑140 (MQ=255)
                                                                                                                             caatttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCg           <  2:5146629/137‑1 (MQ=255)
                                                                                                                               cTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGcc         >  2:1676273/1‑140 (MQ=255)
                                                                                                                                tttAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCa        <  2:3157474/140‑1 (MQ=255)
                                                                                                                                  tcattaactaGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGc      >  2:1304185/3‑140 (MQ=255)
                                                                                                                                    attaactaGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCat    >  1:6359176/1‑140 (MQ=255)
                                                                                                                                    attaactaGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCat    <  2:7720940/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TAACAGGGCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATAT  >  NZ_CP025268/4434952‑4435225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑