BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	383,110	T→C	pseudogene (558/672 nt)	CXP41_RS01990 ←	PIG‑L family deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	383,110	0	T	C	99.5%	1297.6 / NA	378	pseudogene (558/672 nt)	CXP41_RS01990	PIG‑L family deacetylase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (221/155); minor base A (2/0); total (223/155)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 378 total aligned reads displayed.

TCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGG  >  NZ_CP025268/382974‑383246

tCCAGGTTTTCATGCAATTTGCTCACAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAg                                                                                                                                       <  1:8896877/140‑1 (MQ=255)

tCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAg                                                                                                                                       >  1:7605287/1‑140 (MQ=255)

 ccAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGGGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATACTCGCGTCGTTCCTAGc                                                                                                                                      >  2:3875148/1‑140 (MQ=255)

 ccAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGc                                                                                                                                      >  2:8204350/1‑140 (MQ=255)

 ccAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGc                                                                                                                                      >  2:4507569/1‑140 (MQ=255)

 ccAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGc                                                                                                                                      >  2:4866291/1‑140 (MQ=255)

   aGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTCTTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCtt                                                                                                                                    >  2:5966470/1‑140 (MQ=255)

   aGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCtt                                                                                                                                    >  1:1183792/1‑140 (MQ=255)

        ttCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATg                                                                                                                               >  1:1606169/1‑140 (MQ=255)

        ttCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAAGTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTGTTATg                                                                                                                               >  1:2371224/1‑140 (MQ=255)

         tCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGt                                                                                                                              <  1:6931888/140‑1 (MQ=255)

          cATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGtt                                                                                                                             >  1:3771079/1‑140 (MQ=255)

          cATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGtt                                                                                                                             >  1:174833/1‑140 (MQ=255)

                aTTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGCCCTGTTGTCCGCGAAATTGTGCAACTGCTCGCAGGCGATCATGACGCATATACTCGCGTCGTTCCTAGCTTTTATGTTGTTGTa                                                                                                                       >  2:4089672/1‑140 (MQ=255)

                aTTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTa                                                                                                                       >  1:5700206/1‑140 (MQ=255)

                aTTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTa                                                                                                                       >  2:7143841/1‑140 (MQ=255)

                 tttGCTCAAAGAATCATTTTATGAATTACACAGCCTTCACCCAGATCGCAATTGACCAGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTaa                                                                                                                      <  2:6070430/140‑1 (MQ=255)

                 tttGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTaa                                                                                                                      <  1:7300621/140‑1 (MQ=255)

                   tGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATg                                                                                                                    >  2:6650116/1‑140 (MQ=255)

                     cTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATgca                                                                                                                  <  1:9397281/140‑1 (MQ=255)

                     cTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATgca                                                                                                                  <  1:6585678/140‑1 (MQ=255)

                     cTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATgca                                                                                                                  <  2:9023939/140‑1 (MQ=255)

                     cTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATgca                                                                                                                  <  1:8535227/140‑1 (MQ=255)

                       cAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATgcagc                                                                                                                <  2:569674/140‑1 (MQ=255)

                             aTCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGttt                                                                                                          >  1:6497818/1‑140 (MQ=255)

                               cATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGa                                                                                                        <  1:9158098/140‑1 (MQ=255)

                                    tATGAATGACACAGCCTTCACCCATATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACtgtg                                                                                                   <  2:6815588/140‑1 (MQ=255)

                                          ttACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAatat                                                                                             >  2:7896986/1‑140 (MQ=255)

                                           tACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATAtt                                                                                            <  2:338640/140‑1 (MQ=255)

                                           tACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATAtt                                                                                            <  2:5435531/140‑1 (MQ=255)

                                            aCAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATAttc                                                                                           >  2:9366912/1‑140 (MQ=255)

                                            aCAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATAttc                                                                                           >  1:1943710/1‑140 (MQ=255)

                                            aCAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATAttc                                                                                           <  1:3295740/140‑1 (MQ=255)

                                            aCAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTGTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATAttc                                                                                           >  2:2599020/1‑140 (MQ=255)

                                                aGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATtcttct                                                                                       >  1:107148/1‑140 (MQ=255)

                                                aGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATtcttct                                                                                       >  2:8843470/1‑140 (MQ=255)

                                                aGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATtcttct                                                                                       >  1:4429482/1‑140 (MQ=255)

                                                aGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATtcttct                                                                                       >  1:7701454/1‑140 (MQ=255)

                                                     tCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCTACTGCACGCAGGCGATCATGACGCATATAATCGCGTCTTTCCTAGCTTTTATGTTTTTTTAATGCAGCTAGTTTGACTGTGAAATATTCTTCTTTatc                                                                                  >  2:8018082/1‑138 (MQ=255)

                                                     tCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAAc                                                                                  >  2:4211143/1‑140 (MQ=255)

                                                       aCCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACgg                                                                                <  2:7672622/140‑1 (MQ=255)

                                                       aCCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACgg                                                                                <  2:1931014/140‑1 (MQ=255)

                                                       aCCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACgg                                                                                <  2:1213886/140‑1 (MQ=255)

                                                       aCCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACgg                                                                                >  1:4786775/1‑140 (MQ=255)

                                                            gATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCa                                                                           >  1:8107492/1‑140 (MQ=255)

                                                            gATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCa                                                                           >  1:7485447/1‑140 (MQ=255)

                                                            gATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCa                                                                           >  1:2932520/1‑140 (MQ=255)

                                                            gATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCa                                                                           >  1:5236779/1‑140 (MQ=255)

                                                            gATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCAAATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCa                                                                           >  2:8375958/1‑140 (MQ=255)

                                                             aTCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCaa                                                                          <  2:141890/140‑1 (MQ=255)

                                                                 cTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAAc                                                                      >  1:2574799/1‑140 (MQ=255)

                                                                    ttGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTg                                                                   <  2:7363086/140‑1 (MQ=255)

                                                                    ttGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGAATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTg                                                                   <  1:7776984/140‑1 (MQ=255)

                                                                       aCCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAgg                                                                >  1:3505435/1‑140 (MQ=255)

                                                                       aCCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAgg                                                                >  1:4960272/1‑140 (MQ=255)

                                                                         cTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCa                                                              <  2:4553169/140‑1 (MQ=255)

                                                                         cTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCa                                                              <  2:9181066/140‑1 (MQ=255)

                                                                         cTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCa                                                              <  2:7246722/140‑1 (MQ=255)

                                                                            ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATaa                                                           >  1:2084732/1‑140 (MQ=255)

                                                                            ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATaa                                                           >  2:5790868/1‑140 (MQ=255)

                                                                            ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATaa                                                           >  2:2965419/1‑140 (MQ=255)

                                                                            ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCGTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGACATATTCTTCTTTAACGGATTCAAAAACCTGAGGCAGaa                                                           >  2:6945324/1‑140 (MQ=255)

                                                                             tgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATaaa                                                          <  2:6513803/140‑1 (MQ=255)

                                                                                ccGCGACATGGTGCAACTGCACGCCGGCGATCATGACGCATATAATCGCGTCGTTCCTCGCATGTATGTATTAATAATGCAGCAAGTTTGACTGTGAAATATTCGTCTTTAACGGATTCAAAAACCTGAGGCATAAATGa                                                       <  2:8623666/140‑1 (MQ=255)

                                                                                ccGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGa                                                       <  1:6791888/140‑1 (MQ=255)

                                                                                ccGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGa                                                       <  2:1799963/140‑1 (MQ=255)

                                                                                 cgcgAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGaa                                                      >  1:5843027/1‑140 (MQ=255)

                                                                                     aaaTTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGcc                                                  <  1:4674036/140‑1 (MQ=255)

                                                                                     aaaTTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTATTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGcc                                                  <  1:3078106/140‑1 (MQ=255)

                                                                                      aaTTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCa                                                 <  1:604085/140‑1 (MQ=255)

                                                                                      aaTTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCa                                                 <  1:3617337/140‑1 (MQ=255)

                                                                                      aaTTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCa                                                 <  2:1034326/140‑1 (MQ=255)

                                                                                      aaTTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCa                                                 >  2:3068456/1‑140 (MQ=255)

                                                                                      aaTTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTACTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCa                                                 <  1:734928/140‑1 (MQ=255)

                                                                                       aTTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAg                                                <  1:8204350/140‑1 (MQ=255)

                                                                                       aTTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAg                                                <  1:905211/140‑1 (MQ=255)

                                                                                           tgCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTAc                                            >  2:1537490/1‑140 (MQ=255)

                                                                                                cTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTGTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTggg                                       >  2:5007572/1‑140 (MQ=255)

                                                                                                   cACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGtt                                    >  1:4160145/1‑140 (MQ=255)

                                                                                                   cACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGtt                                    >  1:8030362/1‑140 (MQ=255)

                                                                                                                  gACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAAtt                     >  1:7434986/1‑140 (MQ=255)

                                                                                                                    cGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTg                   >  2:5631614/1‑140 (MQ=255)

                                                                                                                        tataATCGCGTCGTTCCTAGGTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGa               >  2:3550311/1‑140 (MQ=255)

                                                                                                                        tataATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCa                                                 >  1:4265073/1‑106 (MQ=255)

                                                                                                                        tataATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCa                                                 <  2:4265073/106‑1 (MQ=255)

                                                                                                                        tataATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAAt                      >  2:5249273/1‑133 (MQ=255)

                                                                                                                        tataATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGa               >  2:7575592/1‑140 (MQ=255)

                                                                                                                        tataATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGa               >  2:2108265/1‑140 (MQ=255)

                                                                                                                        tataATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGa               >  1:5560978/1‑140 (MQ=255)

                                                                                                                        tataATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAAACTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGa               >  1:3374915/1‑140 (MQ=255)

                                                                                                                         ataATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAAt                                                         >  1:4342901/1‑97 (MQ=255)

                                                                                                                         ataATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAAt                                                         <  2:4342901/97‑1 (MQ=255)

                                                                                                                         ataATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGaa              <  2:923529/140‑1 (MQ=255)

                                                                                                                            aTCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAg           >  2:7645559/1‑140 (MQ=255)

                                                                                                                               gagtcgtTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTc        <  1:9244436/138‑1 (MQ=255)

                                                                                                                                cgtcgtTCCTAGGTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCg       >  1:2786490/1‑140 (MQ=255)

                                                                                                                                cgtcgtTCATAGCTTTTATGTTTATTAAATCCAGAAAGTTTGACAGTGAAATATTCTTCTTTAACGGATTCAAAAACATGAGTCATAAATGAAAGACAGGTAATTGGGTTTTAGTATCCGAGAATTTGCGTAATAGTTct       >  2:6997344/1‑139 (MQ=255)

                                                                                                                                 gtcgtTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCgg      >  1:8338269/1‑140 (MQ=255)

                                                                                                                                    gtTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCggcag   <  2:3678256/140‑1 (MQ=255)

                                                                                                                                     ttCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCggcagg  >  1:455600/1‑140 (MQ=255)

TCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAGG  >  NZ_CP025268/382974‑383246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`