Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 4,501,415 A→G *410* (TAA→TAG)  CXP41_RS23285 → IS3‑like element IS2 family transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252684,501,4150AG99.7% 2139.3 / NA 667*410* (TAA→TAG) CXP41_RS23285IS3‑like element IS2 family transposase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base G (232/431);  minor base T (0/2);  total (232/435)
Fisher's exact test for biased strand distribution p-value = 5.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 667 total aligned reads displayed.

TGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGT  >  NZ_CP025268/4501289‑4501551
                                                                                                                              |                                                                                                                                        
ttcAGAGGCTTTCGAGCATTATAACGAATGGAATCCGCATAGTGCTCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGTGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGTAATATAGGGGCAAATCCAcc                                                                                                                             <  2:7867034/138‑1 (MQ=11)
tGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAcc                                                                                                                             >  1:3574319/1‑140 (MQ=11)
tGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAcc                                                                                                                             <  1:3145766/140‑1 (MQ=11)
tGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAcc                                                                                                                             <  1:2121837/140‑1 (MQ=11)
     agGCGTTCGATCATTATAACGAATGGCATCCGCATAGTGAGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTtgtg                                                                                                                        <  2:8537261/140‑1 (MQ=255)
     agGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGCTAACAGATGTCTGGAAATATAGGGGCAAATCCACCTtgtg                                                                                                                        <  1:8737825/140‑1 (MQ=21)
     agGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTtgtg                                                                                                                        <  1:1138913/140‑1 (MQ=21)
     agGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTtgtg                                                                                                                        <  1:756385/140‑1 (MQ=21)
     agGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTtgtg                                                                                                                        <  2:7614592/140‑1 (MQ=21)
     agGAGTTCGAGCATTATAACGAATGGCATCCTCATCTTGCGCTGGGTTATCGCTCGCCACGGTAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACATtgtg                                                                                                                        <  2:2332734/140‑1 (MQ=11)
          ttCGAGCATTATAACGAAGGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGat                                                                                                                   <  2:2600444/140‑1 (MQ=255)
          gtCGAGCATTATAACGAAGGGCATCCGCAGAGTGCGCTGGGTTATCGATCGCCACGGGAATATCTGCGGCAGCGGGCTAGGAATGGGTTACGTGATAACAGAGGGCTGTAAATCTAGGGGCAAATCCACCTTGTGCTGat                                                                                                                   <  2:6475409/139‑1 (MQ=14)
               gCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGaa                                                                                                              <  2:6830637/140‑1 (MQ=255)
               gCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGaa                                                                                                              <  1:1427854/140‑1 (MQ=255)
                   tataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGAGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATAAAGGGGCAAATCCACCTAGTGCTGATAAGAATACg                                                                                                          >  1:1032197/1‑140 (MQ=14)
                    atcaCGAATGGCATCCGCATAGTGCGCTGGGTTATCGCGCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAAGACgg                                                                                                         <  2:1362499/137‑1 (MQ=255)
                    ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                                         <  1:6788585/140‑1 (MQ=255)
                    ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                                         <  2:4156217/140‑1 (MQ=255)
                    ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                                         <  2:5539392/140‑1 (MQ=255)
                    ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                                         <  2:5771874/140‑1 (MQ=255)
                    ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                                         <  2:6155245/140‑1 (MQ=255)
                    ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTAGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                                         <  2:8313580/140‑1 (MQ=255)
                        cGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCAGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGatat                                                                                                     >  1:2037266/1‑140 (MQ=255)
                         gAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATAtt                                                                                                    <  1:552366/140‑1 (MQ=255)
                          aaTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATAttt                                                                                                   >  2:3841118/1‑140 (MQ=255)
                          aaTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATAttt                                                                                                   >  2:3623734/1‑140 (MQ=255)
                          aaTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATAttt                                                                                                   >  2:1166877/1‑140 (MQ=255)
                          aaTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATAttt                                                                                                   >  1:5838225/1‑140 (MQ=255)
                             ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTtat                                                                                                >  2:3480415/1‑140 (MQ=255)
                             ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTtat                                                                                                >  1:986164/1‑140 (MQ=255)
                              gCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCCGCGGGCTTGTAATGGGTTAAGTGATAACAGAGGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTtata                                                                                               <  2:1440602/140‑1 (MQ=255)
                               cATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATAc                                                                                              >  2:1300957/1‑140 (MQ=255)
                                aTCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACa                                                                                             <  1:6110879/140‑1 (MQ=255)
                                aTCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACa                                                                                             <  1:3377712/140‑1 (MQ=255)
                                aTCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGATTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACa                                                                                             <  2:8684191/140‑1 (MQ=255)
                                 tCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAg                                                                                            >  2:2846521/1‑140 (MQ=255)
                                      aTAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATAc                                                                                       <  1:8610401/140‑1 (MQ=255)
                                          tGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACtttt                                                                                   <  2:4296518/140‑1 (MQ=255)
                                          tGCGCGGGGTGATCGCTCCCCACGGGAATATCTGCGGCAGCGGGCTTGTACTGGGTTAAGTGATAACAGATGTCTGGAAATGTAGGGGCAACTCCACCTTGTGCTGATATGAATCCGGATATTTTATACAGAATACtttt                                                                                   <  2:9149352/140‑1 (MQ=18)
                                            cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCa                                                                                 <  1:3009554/140‑1 (MQ=255)
                                            agcTGGGTTATCTCTCGACACGGGAATATCTGCGGCAGAGGGCTTGTAATGGGTTAAGTGATAACAGATGACTGGAAATATAGGGGCACATCCACCTAGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCa                                                                                 >  2:1979089/2‑140 (MQ=18)
                                               tGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTa                                                                              >  2:4455491/1‑140 (MQ=255)
                                                    tATCGCTCGCCACGGTAATATCTGCGGCTGCGGGCTTTTATTGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGAAAATCCACCTTGTGCTGATATGAATAGGGATATTTTATACAGAATACTTTTCAGTACAAGc                                                                         <  1:4755745/140‑1 (MQ=21)
                                                    tATCGCTCGCCACGGTAATAACGGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACATTTGTCTGTAAATATAGGGGCAAATCCACCTTTTGGTTATTTGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGc                                                                         <  2:2175011/140‑1 (MQ=18)
                                                    tATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGTTTATGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACATTGTGCTTATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGc                                                                         <  1:2917661/140‑1 (MQ=21)
                                                    tATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGc                                                                         <  2:4871261/140‑1 (MQ=255)
                                                    tATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGc                                                                         >  1:6906105/1‑140 (MQ=255)
                                                    aaTCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGc                                                                         >  2:6328015/2‑140 (MQ=255)
                                                      tcgctcgcCACGGTAATATCTGCGGCCGCGGGCTTTTAATGGTTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                       <  2:715910/140‑1 (MQ=25)
                                                      tcgctcgcCACGGTAATATCTGCGGCAGCGGGATTGTATTGGGTTAAGTGATAACATATGTCTGTAAATATAGGGGCAAATCCACCTTGTGCTGATATTAATACGGATATTTTATACAGAATAATTTTCAGTACAAGCGa                                                                       <  2:81503/140‑1 (MQ=21)
                                                      tcgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                       <  2:5941381/140‑1 (MQ=255)
                                                      tcgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                       <  1:6526269/140‑1 (MQ=255)
                                                      tcgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                       >  2:1696408/1‑140 (MQ=255)
                                                      tcgcacgcCACGGGAAGATCAGCGCCAGCGGCCTTGTACTGGGTAACGTGATAACAGATGTCTGGAAATATCGGCCCAAATCCACCTTGAGCTGATATGAATACGGCTATTTTATACAGAAAACATTACAGTACAAGCGa                                                                       <  2:4975609/140‑1 (MQ=18)
                                                         ctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATAtt                                                                                                    >  1:3877051/1‑108 (MQ=255)
                                                         ctcgcCACGGGAATATCTGCGGCAGCGGGATTGTCATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACCGTATTCATATCAGCACAAggtggat                                                                    <  2:4012100/140‑7 (MQ=18)
                                                           cgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATa                                                                                        >  2:8955496/1‑118 (MQ=255)
                                                                  ggAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATACCAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGt                                                           <  1:4428485/140‑1 (MQ=255)
                                                                  ggAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACTGATGTCTGTAAATATAGGGCAAAATCCACCTTGTGCTGTTATGAATACGGATTTTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGt                                                           <  1:1533438/140‑1 (MQ=25)
                                                                  ggAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGt                                                           <  2:303898/140‑1 (MQ=255)
                                                                     atatCTGCGTCAGCGGGCTTGTAATGGGTTATGTGATAACAGATGTCTGGAAATATATGGGCAAATCCACCATGTGATGATATGAATACGGATATTTTATACAGAATAATTTTCTGTACAAGCGACTGTATTACAGTCTg                                                        <  2:8073642/140‑1 (MQ=25)
                                                                     atatCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTg                                                        <  1:7952627/140‑1 (MQ=255)
                                                                     atatCGGCGGCAGCGGGCTTGTATTGGGTTAAGTGAAAACAGATGTCGGGAAATATAGGGGCAAACCCACCTTGTGCTTATATGAAAACGGATATTTTATACAGAATACTTTTCAGAACAAGCGACTGTATTACAGTCTg                                                        <  1:8126102/140‑1 (MQ=255)
                                                                        tCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCa                                                     >  1:9310088/1‑140 (MQ=255)
                                                                        tCTGCGGCAGCGGGATTGTAATGGGTTAAGTTATAACAGATGTCTGGAAATATAGGGGAAAATCCTCCTTGTGCTGATATGAATACGGATATTTTATACATAATACTTTTCTTTACAAGCGACTGTATTACAGTCTGCCa                                                     <  2:1829556/140‑1 (MQ=25)
                                                                           gCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCatat                                                  <  2:9044602/140‑1 (MQ=255)
                                                                             ggCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCatatat                                                >  1:4028398/1‑140 (MQ=255)
                                                                             ggCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCatatat                                                <  2:667382/140‑1 (MQ=255)
                                                                                    ggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                       >  1:8958258/1‑110 (MQ=255)
                                                                                        tttactGGTTTAAGTTATAACTGATTTCTGGAAATTTAGGGGCAAATCCACATTGTGCTTATATGAATACGGATATTTTATACATAATACTTTTCATTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCtgtg                                     <  1:7373096/135‑1 (MQ=255)
                                                                                         gTAATGGGTTAAGTGATAAAAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATAAAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGa                                    <  2:1568001/140‑1 (MQ=255)
                                                                                          tAATGGGTTAAGTTATAACAGATTTATGTAAATATAGGGGCAAATCAACCTTTTGATGTTATTAATACGGATATTTTATACAGAATACTGTTCTGTAAAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGAt                                   <  2:3145798/140‑1 (MQ=255)
                                                                                               ggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTa                                                               <  2:9297838/107‑1 (MQ=255)
                                                                                               ggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGga                              >  2:7794388/1‑140 (MQ=255)
                                                                                                gTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                       <  2:6198644/98‑1 (MQ=255)
                                                                                                gTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaa                             <  2:7975303/140‑1 (MQ=255)
                                                                                                gTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaa                             <  1:5761122/140‑1 (MQ=255)
                                                                                                 ttAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaag                            >  2:7271808/1‑140 (MQ=255)
                                                                                                    aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCt                                                                                                                      >  1:2502105/1‑47 (MQ=255)
                                                                                                    aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCt                                                                                                                      <  2:2502105/47‑1 (MQ=255)
                                                                                                    aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGc                                                                         <  2:8841533/92‑1 (MQ=255)
                                                                                                    aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAAt                         >  1:6376947/1‑140 (MQ=255)
                                                                                                    aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAAt                         >  1:8315992/1‑140 (MQ=255)
                                                                                                    aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAAt                         >  2:3328937/1‑140 (MQ=255)
                                                                                                           aCAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTg                                                        <  1:5112698/102‑1 (MQ=255)
                                                                                                           aCAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGc                  >  1:718568/1‑140 (MQ=255)
                                                                                                             agttGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTg                <  1:1924043/137‑1 (MQ=255)
                                                                                                              gATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGa               >  1:2182313/1‑140 (MQ=255)
                                                                                                               aTGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCtg                                       >  1:3773132/1‑115 (MQ=255)
                                                                                                               aTGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAg              >  1:4630230/1‑140 (MQ=255)
                                                                                                               aTGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAg              >  1:9191808/1‑140 (MQ=255)
                                                                                                               aTGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAg              >  1:5309811/1‑140 (MQ=255)
                                                                                                               aTGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAg              >  2:3748416/1‑140 (MQ=255)
                                                                                                               aTGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAg              >  2:461035/1‑140 (MQ=255)
                                                                                                                tGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAgg             >  2:5315030/1‑140 (MQ=255)
                                                                                                                    tGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTg                                                        >  1:2409112/1‑93 (MQ=255)
                                                                                                                    tGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTc         >  1:4189324/1‑140 (MQ=255)
                                                                                                                    tGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTc         <  1:7794388/140‑1 (MQ=255)
                                                                                                                     ggAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCa        <  1:1760508/140‑1 (MQ=255)
                                                                                                                       aaaTATTGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCaaa      <  1:5005674/140‑1 (MQ=255)
                                                                                                                              |                                                                                                                                        
TGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGT  >  NZ_CP025268/4501289‑4501551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑