BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	356,677	2 bp→CC	intergenic (+172/+164)	codA → / ← cynR	cytosine/isoguanine deaminase/transcriptional regulator CynR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	356,677	0	T	C	97.5%	1357.9 / NA	454	intergenic (+172/+165)	codA/cynR	cytosine/isoguanine deaminase/transcriptional regulator CynR
Reads supporting (aligned to +/- strand): ref base T (1/0); major base C (165/273); minor base A (9/2); total (178/276)
Fisher's exact test for biased strand distribution p-value = 4.19e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NZ_CP025268	356,678	0	A	C	97.1%	1381.1 / ‑1.2	454	intergenic (+173/+164)	codA/cynR	cytosine/isoguanine deaminase/transcriptional regulator CynR
Reads supporting (aligned to +/- strand): ref base A (12/1); new base C (166/275); total (178/276)
Fisher's exact test for biased strand distribution p-value = 8.88e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 454 total aligned reads displayed.

ATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAG  >  NZ_CP025268/356540‑356811

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      cgTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTACCGCCAACAACCGCGCATTACTCCAACg                                                                                                                                >  1:1007447/1‑140 (MQ=255)

                ttATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCaa                                                                                                                      >  2:5231552/1‑140 (MQ=255)

                   tCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCaaaaa                                                                                                                   <  2:3103030/140‑1 (MQ=255)

                       gCCTACGTAGAGCACTGAACTCGTAGGCCTGATAATCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGcc                                                                                                               >  1:933275/1‑140 (MQ=11)

                       gCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGcc                                                                                                               <  1:8255102/140‑1 (MQ=255)

                       gCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTACTCCAACGCCATGTGCAAAAATGcc                                                                                                               >  1:8088706/1‑140 (MQ=255)

                       gCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTAATCCAACGCCATGTGCAAAAATGcc                                                                                                               >  1:3041799/1‑140 (MQ=255)

                         cTACGGAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCtt                                                                                                             <  2:8062216/140‑1 (MQ=11)

                                 agCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTGATCCAACGCCATGTGCAAAAATGCCTTCGcggcgg                                                                                                     >  2:3973039/1‑140 (MQ=255)

                                  gCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCTGCGGc                                                                                                    >  1:2869132/1‑140 (MQ=11)

                                  gCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGc                                                                                                    <  1:5775383/140‑1 (MQ=255)

                                   cACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCt                                                                                                   >  1:9185973/1‑140 (MQ=255)

                                      tGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTc                                                                                                >  1:3733766/1‑140 (MQ=255)

                                      tGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATGCCTCCCACGCCATGTGCAAAAATGCCTGCGCCGCGGCTGTc                                                                                                >  2:6498318/1‑140 (MQ=11)

                                       gAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTACTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCt                                                                                               >  1:4938805/1‑140 (MQ=255)

                                        aaCTCGTAGGCCTTATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTg                                                                                              >  1:470641/1‑140 (MQ=11)

                                        aaCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTg                                                                                              >  1:3242243/1‑140 (MQ=255)

                                         aCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGc                                                                                             <  1:143389/140‑1 (MQ=255)

                                         aCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGc                                                                                             <  1:1764723/140‑1 (MQ=255)

                                           tCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCa                                                                                           >  1:8974404/1‑140 (MQ=255)

                                           tCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCa                                                                                           <  1:7114636/140‑1 (MQ=255)

                                           tCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTACCGCCAACAACCGCGCATTACTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCa                                                                                           >  2:3673875/1‑140 (MQ=255)

                                              tAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCt                                                                                        <  1:8871332/140‑1 (MQ=255)

                                                  ccTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCtatat                                                                                    >  2:821667/1‑139 (MQ=11)

                                                  ccTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAg                                                                                    >  1:8741422/1‑140 (MQ=255)

                                                  ccTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGAGCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAg                                                                                    >  2:8798231/1‑140 (MQ=11)

                                                      aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTa                                                                                >  1:5536222/1‑140 (MQ=255)

                                                           cGTAGCGCATCAGGCAATACCAGCCGCTGATATGTGTCAGCGGCTACCGTGATTAATTCCCGCCAACAACCGCTCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTATAGATAATGCCg                                                                           >  2:9009786/1‑140 (MQ=14)

                                                             tAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGa                                                                         >  2:2048339/1‑140 (MQ=255)

                                                             tAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGGTGCTGCCGGa                                                                         >  2:9278793/1‑140 (MQ=14)

                                                             tAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTACTCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTTTAGTTGATGCCgta                                                                         >  2:5774623/1‑138 (MQ=11)

                                                               gcgcATCACGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCTCCAACTACCTAGCAGTCCTCCTACGCCAAGTTCAACTCTTCATTCGCAGCGGATGTCTGGCAGCTGTAGTTTATGCCGGATg                                                                       <  1:4009610/140‑1 (MQ=255)

                                                                cgcATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgc                                                                      <  1:1877686/140‑1 (MQ=255)

                                                                   aTCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgcggc                                                                   >  2:1220709/1‑140 (MQ=255)

                                                                     cAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGcc                                                                                                                              >  1:8559215/1‑79 (MQ=255)

                                                                      aGGCATTTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACACCCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTg                                                                <  2:7666287/140‑1 (MQ=18)

                                                                      aGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTg                                                                <  2:6663093/140‑1 (MQ=255)

                                                                      aGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTg                                                                <  2:5175157/140‑1 (MQ=255)

                                                                       ggCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGa                                                               >  2:738788/1‑140 (MQ=255)

                                                                       ggCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGa                                                               <  1:7715404/140‑1 (MQ=255)

                                                                       ggCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGa                                                               <  1:2354495/140‑1 (MQ=255)

                                                                           aTTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGc                                                           <  1:2813518/140‑1 (MQ=255)

                                                                            ttCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGcc                                                          >  1:2208266/1‑140 (MQ=255)

                                                                                aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCACCAACCGCGCATTCCTCCAACGCCATGTGCAAACATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCttcg                                                      <  2:2519347/140‑3 (MQ=18)

                                                                                aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGNCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                      <  1:6284588/140‑1 (MQ=255)

                                                                                aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCTGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                      <  1:1587944/140‑1 (MQ=21)

                                                                                aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                      <  1:2630549/140‑1 (MQ=255)

                                                                                aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                      <  1:3346410/140‑1 (MQ=255)

                                                                                aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                      <  2:2774241/140‑1 (MQ=255)

                                                                                aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                      <  2:6297708/140‑1 (MQ=255)

                                                                                aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                      <  1:655794/140‑1 (MQ=255)

                                                                                aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                      <  1:1171101/140‑1 (MQ=255)

                                                                                aGCCGCTGATCGGTGTCCGCGGCTACCGTGATTCAGTCCCGCCCCCAACCGCGCATTCCTCCCACGCCATGTGCAAAAAAGCCTTCGCAGCGGCTGTCCGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                      <  1:5668573/140‑1 (MQ=255)

                                                                                  ccGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATcc                                                    <  2:1698017/140‑1 (MQ=255)

                                                                                  ccGCTGATCTGTGTCAGCGGCTACCGTGATTCAGTCCCGCCAACAACCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATcc                                                    <  1:9399821/140‑1 (MQ=21)

                                                                                     cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                 <  2:2315752/140‑1 (MQ=255)

                                                                                     cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                 <  2:3590374/140‑1 (MQ=255)

                                                                                     cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                 <  1:3879100/140‑1 (MQ=255)

                                                                                     cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                 <  1:8327290/140‑1 (MQ=255)

                                                                                      tGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCCTGAACGCCTTATCCGGcc                                                >  2:191277/1‑140 (MQ=21)

                                                                                       gATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                               <  2:4662709/140‑1 (MQ=255)

                                                                                       gATCTGTGTCAGCGGCTACCGTGATTCATTCACGCCAACAACAGCGCTTTCCTCGAACGCCATGTGGAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                               <  2:5510361/140‑1 (MQ=37)

                                                                                         tCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc                                             <  2:829306/140‑1 (MQ=255)

                                                                                          cTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACg                                            <  1:5207521/140‑1 (MQ=255)

                                                                                          cTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACg                                            <  1:738788/140‑1 (MQ=255)

                                                                                           tgtgTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGt                                           >  1:1702684/1‑140 (MQ=255)

                                                                                            gtgtCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTa                                          <  2:8664300/140‑1 (MQ=255)

                                                                                            gtgtCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATCGCTCGTACGCCTCGTGATAATATGCCTTGTCAGCGGGTGTAATCCTTCAGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTa                                          <  2:100984/140‑1 (MQ=16)

                                                                                             tgtCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                 <  1:6631051/132‑1 (MQ=255)

                                                                                             tgtCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                 <  1:7554667/132‑1 (MQ=255)

                                                                                             tgtCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTag                                         <  2:7153055/140‑1 (MQ=255)

                                                                                             tgtCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGag                                         >  1:531341/1‑140 (MQ=21)

                                                                                               tCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                      >  2:2868164/1‑125 (MQ=255)

                                                                                                 aGCGGCTACCGTGATTCACTCCCGCCAACAACCGCGCAGTCCTCCAACGCCATGGGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTAGGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCa                                     <  2:79742/140‑1 (MQ=37)

                                                                                                    ggctACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTCTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTg                                  >  2:3256115/4‑140 (MQ=25)

                                                                                                    ggctACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTg                                  <  2:441598/137‑1 (MQ=255)

                                                                                                     gctACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGa                                 <  2:3328209/138‑1 (MQ=255)

                                                                                                     gctACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGa                                 <  1:4233472/138‑1 (MQ=255)

                                                                                                     gctACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGa                                 <  2:7084232/138‑1 (MQ=255)

                                                                                                       tACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAAc                               >  1:3524389/1‑140 (MQ=255)

                                                                                                       tACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTCTATTTAATCCCGTAAGAGGCGTGATATCAATATCCTGCATACGGAGAGCACTGAAc                               >  2:2941757/1‑140 (MQ=9)

                                                                                                          cGTGATTCATTCCCGCCACCAACCGCGCAGTCCTCCACCGCCATGTGCAACAATGCCTTCGCAGCGGCGGTCTGCCAGCTGGAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCg                            <  2:8413434/140‑1 (MQ=25)

                                                                                                             gATTCAGTCCCGCCAACAACCGCGCATTCCTCCCACGCCATGGGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAg                         <  1:9455250/140‑1 (MQ=25)

                                                                                                              attcattcCCGCCAACAAACGCGCATTCCTCCAACGCCATGAGCAAAAATGCCTTCGCATCGGCTGACTGCCAGCTGTAGTTTATGCCCGATGCGGCGTGAACGCCTTATCCGGCCTACGTCGAGCACTGAACACGTAgg                        >  1:5920354/1‑140 (MQ=25)

                                                                                                               ttCATTTCCGCCAACAACCGCGCAGTCCTCCAACGCCATGTGCAAAACTGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTTCGTTGAGCACTGATCTCGTAGGc                       <  2:4940058/140‑1 (MQ=25)

                                                                                                               ttCATTCCCGCCAACACCCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGc                       <  1:6872893/140‑1 (MQ=255)

                                                                                                               ttCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGc                       <  1:1220709/140‑1 (MQ=255)

                                                                                                               ttCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGc                       <  2:1480411/140‑1 (MQ=255)

                                                                                                                   ttcccGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCCGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGAGGCGGCGTGACCGCCATAGCCGGCCTACGTAGAGCACTGAACTCGTAGGccaca                   >  1:4377840/4‑137 (MQ=25)

                                                                                                                   ttcccGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGa                   >  1:7898846/4‑140 (MQ=255)

                                                                                                                    tcccGCCACCAACCGCGCAGTCCTCCACCGCCATGTGCAAAAATGCCTTCGCAGCTGCTGTCTGCCAGCTGTAGTTTATGCCGGCTGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGAt                  <  2:6189693/138‑1 (MQ=21)

                                                                                                                    tcccGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGAt                  <  1:4470117/138‑1 (MQ=255)

                                                                                                                    tcccGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGAt                  <  1:8392588/138‑1 (MQ=255)

                                                                                                                    tcccGCCAACAACCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGAt                  <  2:3870993/138‑1 (MQ=255)

                                                                                                                     cccGCCAACACCCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATa                 <  1:5075588/139‑1 (MQ=255)

                                                                                                                           accaacCGCGCATTCCTCCCACGCCATGTGCAACAATGCCTTCGCAGCGGCTGTCAGCCAGCGGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTa           <  1:1926951/138‑1 (MQ=21)

                                                                                                                           aacaacCGCGCAGACCTCCCACGCCATGTGCAAACATGCCTTCGCAGCGGCTGTCTGCCAGCGGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTa           <  1:4071034/140‑1 (MQ=21)

                                                                                                                                ccGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTAATCCCGGAAGCGGCGTGAACGCCTTATCCGGCATACGGAGAGCGAGGAAGTCGTAGGCCTGATAAGAGTAgagcg      >  2:7806596/1‑139 (MQ=21)

                                                                                                                                   cgcATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCg                            <  1:1088488/115‑1 (MQ=255)

                                                                                                                                    gcATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAg  <  2:1568517/140‑1 (MQ=255)

||

ATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAG  >  NZ_CP025268/356540‑356811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`