Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 4,477,488 (G)7→6 pseudogene (127/1816 nt) CXP41_RS23185 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252684,477,4820G.99.8% 2959.5 / ‑3.5 614pseudogene (121/1816 nt)CXP41_RS23185hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base . (299/314);  total (299/315)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 694 total aligned reads displayed.

TTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATT  >  NZ_CP025268/4477343‑4477617
                                                                                                                                           |                                                                                                                                       
    cTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCggggg                                                                                                                                     >  2:8248398/1‑140 (MQ=255)
     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    <  1:3781150/140‑1 (MQ=255)
     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    >  1:7350971/1‑140 (MQ=255)
     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    >  1:7200345/1‑140 (MQ=255)
     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    >  1:8010995/1‑140 (MQ=255)
     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    >  1:5577330/1‑140 (MQ=255)
     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    >  2:2382341/1‑140 (MQ=255)
     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    <  1:1214604/140‑1 (MQ=255)
     tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    <  2:4900479/140‑1 (MQ=255)
       aaGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCggggggca                                                                                                                                  <  2:7510612/140‑3 (MQ=255)
       aaGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCggggggca                                                                                                                                  >  1:8403506/1‑138 (MQ=255)
       aaGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCggggggca                                                                                                                                  >  2:8785956/1‑138 (MQ=255)
       aaGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCggggggca                                                                                                                                  >  2:8538647/1‑138 (MQ=255)
             ttCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATaa                                                                                                                           >  1:4734470/1‑140 (MQ=255)
             ttCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAAGGATTTTGCCCGTATTGCC‑GGGGGGCAGGATaa                                                                                                                           >  2:6383615/1‑140 (MQ=255)
               tgTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACa                                                                                                                         >  1:7460465/2‑140 (MQ=255)
               cGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACa                                                                                                                         >  1:324393/1‑140 (MQ=255)
               cGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACa                                                                                                                         >  2:1897697/1‑140 (MQ=255)
               cGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACa                                                                                                                         >  1:9354476/1‑140 (MQ=255)
               cGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACa                                                                                                                         >  2:8347527/1‑140 (MQ=255)
               cGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACa                                                                                                                         >  1:3928767/1‑140 (MQ=255)
               cGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACa                                                                                                                         >  2:9274051/1‑140 (MQ=255)
                gTCAGGAGCAAACTATCAGATTGAAATTATTCTCCACACATGACCTTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAg                                                                                                                        <  2:6742999/140‑1 (MQ=255)
                      aGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGaa                                                                                                                  <  2:5737265/140‑1 (MQ=255)
                                  gATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTcc                                                                                                      >  2:549363/1‑140 (MQ=255)
                                       aaaaTAGTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAg                                                                                                 <  1:9588052/140‑1 (MQ=255)
                                       aaaTTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAg                                                                                                 >  2:2278305/1‑140 (MQ=255)
                                       aaaTTAGTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAg                                                                                                 <  1:4923909/140‑1 (MQ=255)
                                             ttCTCAACACATTGCATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCAtttt                                                                                           >  1:9184475/1‑140 (MQ=255)
                                             ttCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGTATATTCTTTCTCCTGGAGCAtttt                                                                                           >  2:4530645/1‑140 (MQ=255)
                                             ttCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCAtttt                                                                                           >  1:3612343/1‑140 (MQ=255)
                                               ctcAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTa                                                                                         <  1:8158332/140‑1 (MQ=255)
                                                  aaCACATTACATTAGCCGACAATTTTAAACAAAATAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTagag                                                                                      >  1:8571936/1‑140 (MQ=255)
                                                  aaCACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTagag                                                                                      >  1:3395034/1‑140 (MQ=255)
                                                  aaCACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATGTTTagag                                                                                      >  2:6267865/1‑140 (MQ=255)
                                                  aaCACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAAGGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTagag                                                                                      >  1:1066987/1‑140 (MQ=255)
                                                      cattacattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGtttt                                                                                  <  1:792610/140‑1 (MQ=255)
                                                       attacattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGttttt                                                                                 >  2:3583443/1‑140 (MQ=255)
                                                       attacattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGttttt                                                                                 >  1:6918477/1‑140 (MQ=255)
                                                       attacattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGttttt                                                                                 >  1:1793815/1‑140 (MQ=255)
                                                       attacattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGttttt                                                                                 >  1:5792136/1‑140 (MQ=255)
                                                       attacattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGttttt                                                                                 >  1:2960236/1‑140 (MQ=255)
                                                       attacattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGttttt                                                                                 >  1:1580300/1‑140 (MQ=255)
                                                           cattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCa                                                                             <  2:6043696/140‑1 (MQ=255)
                                                           cattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAG‑TTTTGGCaa                                                                            <  2:3762645/140‑1 (MQ=255)
                                                            attaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaa                                                                            >  1:9008144/1‑140 (MQ=255)
                                                             ttaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                           <  1:7705703/140‑1 (MQ=255)
                                                             ttaGCCGACAATTATAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                           <  1:5460666/140‑1 (MQ=255)
                                                                gCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATCACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGat                                                                        >  2:3113772/1‑140 (MQ=255)
                                                                gCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTTGCAAAGat                                                                        >  1:633534/1‑140 (MQ=255)
                                                                gCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCa                                                                             <  2:7832615/135‑1 (MQ=255)
                                                                gCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGat                                                                        >  1:2388775/1‑140 (MQ=255)
                                                                gCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGat                                                                        >  2:4746885/1‑140 (MQ=255)
                                                                 ccGACAATTTTAAACATAAAAGTGAAGTTTTTTATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATTTTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGata                                                                       <  1:5089502/140‑1 (MQ=255)
                                                                 ccGACAATTTTAAACAAAAAAGTGAAGTTTTGAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGata                                                                       >  2:7238855/1‑140 (MQ=255)
                                                                 ccGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAGAGata                                                                       >  2:1519747/1‑140 (MQ=255)
                                                                 ccGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGata                                                                       >  1:1458298/1‑140 (MQ=255)
                                                                 ccGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGata                                                                       >  2:1365202/1‑140 (MQ=255)
                                                                  cGACAATTTTAAACAAAAAAGTGAAGTTTTAATTACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGATTATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGatat                                                                      <  2:9407750/140‑1 (MQ=255)
                                                                  cGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGatat                                                                      <  2:6931887/140‑1 (MQ=255)
                                                                  cGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGatat                                                                      <  1:6580800/140‑1 (MQ=255)
                                                                  cGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGatat                                                                      <  1:5185250/140‑1 (MQ=255)
                                                                  cGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGatat                                                                      <  2:9186816/140‑1 (MQ=255)
                                                                  cGACAATATGACACTAAACAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATATTGCCCGTATTGCC‑GGGGGGCAGGAGAACAGAAGGCATATTCGTTCTCCTGGAGCATTTTTAGAGATTTTGGCAAAGatat                                                                      <  2:4115704/140‑1 (MQ=255)
                                                                     cAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTa                                                                   >  1:1982626/1‑140 (MQ=255)
                                                                       aTTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTAcc                                                                 <  2:8938572/140‑1 (MQ=255)
                                                                                             tttAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCa                                           <  2:4380278/140‑1 (MQ=255)
                                                                                             tatAAATACCTGGCGTGTTGGAATGAATGATAGTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAAGATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCa                                           <  2:9624954/138‑1 (MQ=255)
                                                                                               tAAATACCTGGCGTTTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGTATAACAGATGGAATATTCTTTCTCCTGGAGCATTTTTAGATTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCaaa                                         <  1:2237230/140‑1 (MQ=255)
                                                                                               tAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCaaa                                         <  2:5280152/140‑1 (MQ=255)
                                                                                               tAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCaaa                                         <  1:6020930/140‑1 (MQ=255)
                                                                                                 aaTACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTGTTAGAGTTTTTGGCAAAGATCTTTACCCTGGGTTATGTGGATTTTAGCAAACg                                       >  2:4232093/1‑140 (MQ=255)
                                                                                                      cTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCa                                  >  2:3906768/1‑140 (MQ=255)
                                                                                                      cTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCa                                  >  2:3319401/1‑140 (MQ=255)
                                                                                                        ggCGTGTTGGAATGAATGATTTTGCCCGTATTGCCgggggg                                                                                                                                    >  2:2097205/1‑41 (MQ=255)
                                                                                                        ggCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAAc                                >  2:9517227/1‑140 (MQ=255)
                                                                                                        ggCGTGTTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAAc                                >  1:4192304/1‑140 (MQ=255)
                                                                                                           gtgtTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGaa                             >  2:2508054/1‑140 (MQ=255)
                                                                                                            tgtTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAg                            <  1:8296099/140‑1 (MQ=255)
                                                                                                            tgtTGGAATGAATGATTTTGCCCGTAGTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAACCGCTCCAACGAAg                            <  2:8117672/140‑1 (MQ=255)
                                                                                                             gtTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGc                           <  2:9744318/140‑1 (MQ=255)
                                                                                                             gtTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGc                           <  1:2599159/140‑1 (MQ=255)
                                                                                                             gtTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGc                           <  1:9658331/140‑1 (MQ=255)
                                                                                                             gtTGGAATGAATGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGc                           <  2:1635789/140‑1 (MQ=255)
                                                                                                                 gaatgaatGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGt                       <  2:3413582/140‑1 (MQ=255)
                                                                                                                 gaatgaatGATTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAAGATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGt                       <  1:9754591/140‑1 (MQ=255)
                                                                                                                      aatgaTTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGATAGaaa                  >  1:1339777/1‑140 (MQ=255)
                                                                                                                      aatgaTTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGATAGaaa                  >  1:8909765/1‑140 (MQ=255)
                                                                                                                        tgaTTTTGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAata                >  1:3531747/1‑140 (MQ=255)
                                                                                                                           ttttGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATatga             <  1:9850115/140‑1 (MQ=255)
                                                                                                                           ttttGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATatga             >  1:247173/1‑140 (MQ=255)
                                                                                                                            tttGCCCGTATTGCC‑GGGGGGCAGGANAACAGAANGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAANGAAGCGGGTAGAAATatgat            <  1:6241029/140‑1 (MQ=255)
                                                                                                                              tGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATgg          <  2:5910267/140‑1 (MQ=255)
                                                                                                                              tGCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGACTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATgg          <  1:4397114/140‑1 (MQ=255)
                                                                                                                               gCCCGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGc         <  2:506743/140‑1 (MQ=255)
                                                                                                                                cccGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCt        <  2:1066987/140‑1 (MQ=255)
                                                                                                                                cccGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCt        <  2:3018589/140‑1 (MQ=255)
                                                                                                                                 ccGTATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTc       <  1:30840/140‑1 (MQ=255)
                                                                                                                                    tATTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCata    >  2:6531065/1‑140 (MQ=255)
                                                                                                                                     aTTGCC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCatat   >  2:2779992/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                       
TTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATT  >  NZ_CP025268/4477343‑4477617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑