BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,146,507	+T	pseudogene (1028/1643 nt)	flgK →	flagellar hook‑associated protein FlgK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,146,507	1	.	T	98.8%	1952.3 / 9.5	507	pseudogene (1028/1643 nt)	flgK	flagellar hook‑associated protein FlgK
Reads supporting (aligned to +/- strand): ref base . (3/3); new base T (220/280); total (223/284)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 545 total aligned reads displayed.

ACGCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑G‑GACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGACGCCGGAT  >  NZ_CP025268/1146368‑1146639

 cGCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACACAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gt                                                                                                                                      >  1:3267978/1‑139 (MQ=255)

 cGCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gt                                                                                                                                      >  2:2280070/1‑139 (MQ=255)

 cGCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCAGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTCTCGGTAAGCCCGCGGTTCTGCAAATCACGAAGAACAAA‑g‑gt                                                                                                                                      >  1:8569942/1‑139 (MQ=255)

    ttGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gtgac                                                                                                                                   >  2:6320833/1‑136 (MQ=255)

    ttGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gtgac                                                                                                                                   <  1:3521397/140‑5 (MQ=255)

    ttGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gtgac                                                                                                                                   >  1:3376482/1‑136 (MQ=255)

       gACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGtt                                                                                                                                >  2:9329546/1‑140 (MQ=255)

        aCAACTGGCGCTGGCATGTTCCGAGGCTTTCAACACCCAACACACAGCCGGATTGGATTCGAACGTCGAGGCCGGTGAAGATTTCTTTGCTAGCGGTAAGCCCGCGGTGCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTg                                                                                                                               <  2:197654/140‑1 (MQ=255)

           aCTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCCAAACACGAAAAACAAA‑G‑GTGACGTTGCGa                                                                                                                            >  2:5901157/1‑140 (MQ=255)

            cTGGCTCTGGCATTTGCCGAGTCTTTAAACACCCAACACAAAGCCGGATTTGATTCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  2:3625829/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  1:675775/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  2:510140/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           >  1:8947864/1‑140 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTGGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  2:2963827/140‑1 (MQ=255)

             tGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATc                                                                                                                          >  1:3983658/1‑140 (MQ=255)

              ggCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCg                                                                                                                         >  1:2907924/1‑140 (MQ=255)

                cgcTGGCATTTGCCGAGGCTTTCAACACCCCACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGt                                                                                                                       <  2:2150690/140‑1 (MQ=255)

                cgcTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGt                                                                                                                       <  2:2490425/140‑1 (MQ=255)

                cgcTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGt                                                                                                                       <  1:2005732/140‑1 (MQ=255)

                  cTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGc                                                                                                                     >  2:5433723/1‑140 (MQ=255)

                  cTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGGTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGc                                                                                                                     <  1:3005964/140‑1 (MQ=255)

                   tGGCAGTTGCCGCGGCTGACACCCCCCACCACCAAGCCGGACTTGATCCTAACGGCGATGCCGGTGCAGATTTCTGTGCTATCGGTACGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGGTGCGATCGGTGcc                                                                                                                    <  2:8488679/140‑1 (MQ=255)

                    ggCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCa                                                                                                                   >  1:380117/1‑140 (MQ=255)

                    ggCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCa                                                                                                                   >  2:3149210/1‑140 (MQ=255)

                      cATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACg                                                                                                                 >  1:8260585/1‑140 (MQ=255)

                      cATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACg                                                                                                                 >  1:9372309/1‑140 (MQ=255)

                        tttGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGt                                                                                                               <  2:9043012/140‑1 (MQ=255)

                           gCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGAGCCACGGTAAc                                                                                                            >  2:3041338/1‑140 (MQ=255)

                              gAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAATATTTCATTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGa                                                                                                         <  2:1499603/140‑1 (MQ=255)

                               atgCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGAt                                                                                                        <  1:3742906/138‑1 (MQ=255)

                               aGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑GTT‑GACGTTGCGATCGGTGCCACGGTAACTGAt                                                                                                        <  2:4411945/140‑1 (MQ=255)

                               aGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGAt                                                                                                        >  2:5599473/1‑140 (MQ=255)

                               aGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAAAACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGACACGGTAAATGAt                                                                                                        >  1:2253241/1‑140 (MQ=255)

                                 gCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGc                                                                                                      <  1:5433723/140‑1 (MQ=255)

                                 gCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGc                                                                                                      >  1:6965938/1‑140 (MQ=255)

                                 gCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGc                                                                                                      <  1:7892736/140‑1 (MQ=255)

                                 gCTCTCAACACCCAACACACAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGc                                                                                                      <  1:8062987/140‑1 (MQ=255)

                                     tCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAATAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTcc                                                                                                  >  2:6031308/1‑140 (MQ=255)

                                      cAACACCGTACACAAAGCCGGATTTGATGCTAACGGGGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCCATACACGAAAATCATA‑G‑GTGACGTTGCGATCGGTGCCACGGTCACTGATGCCTCcg                                                                                                 >  2:2603081/1‑140 (MQ=255)

                                      cAACACCCAACACTAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCGCGCGGTTCTGCAAAACACGATTAACATT‑G‑GTGACGTTGCGATCGGTGCGACGGTTACTGATGCCTCcg                                                                                                 <  2:1800015/140‑1 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 <  2:6520367/140‑1 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 <  2:5973392/140‑1 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 <  1:91768/140‑1 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 <  2:4835112/140‑1 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCAGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 <  1:6177219/140‑1 (MQ=255)

                                        acacCCAACACAAAGCCGGATTTGATGCTAACGTCGATGCCGTTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTTCAAAACACGAACAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcgcg                                                                                               <  2:9496594/140‑1 (MQ=255)

                                           cccAACACAAAGCCGGATTTGATGCTCACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTa                                                                                            <  2:9195171/140‑1 (MQ=255)

                                           cccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTa                                                                                            <  2:7170775/140‑1 (MQ=255)

                                            ccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTAc                                                                                           >  2:83931/1‑140 (MQ=255)

                                            ccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTAc                                                                                           >  2:7954080/1‑140 (MQ=255)

                                              aaCACAAAGCCGGATTTGATGCTACCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGATGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTg                                                                                         >  1:7349929/1‑140 (MQ=255)

                                               acacAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTgg                                                                                        >  1:1566835/1‑140 (MQ=255)

                                                  caAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGa                                                                                     <  2:7734522/140‑1 (MQ=255)

                                                  caAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGa                                                                                     <  1:192419/140‑1 (MQ=255)

                                                  caAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGa                                                                                     <  1:4685946/140‑1 (MQ=255)

                                                     aGCCGGATTTGATNGTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCGACGGTAANTGATGCCTCCGCGGTACTGGCGACAg                                                                                  >  1:6251680/1‑140 (MQ=255)

                                                      gCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGa                                                                                 >  1:9544580/1‑140 (MQ=255)

                                                      gCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGa                                                                                 <  1:8666723/140‑1 (MQ=255)

                                                        cGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAATG‑T‑GACGTTGCGATCGGTGCCACGGTAACTTATGCCTCCGAGGTACTGGCGACAGAtt                                                                               >  2:5271379/1‑140 (MQ=255)

                                                                aTGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAAtc                                                                       <  2:2774102/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  1:6538012/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  2:7541648/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  1:1005232/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  1:9487395/140‑1 (MQ=255)

                                                                          cGATGCCGGTGATGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGata                                                             <  2:431822/140‑1 (MQ=255)

                                                                           gATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGataa                                                            >  1:5637130/1‑140 (MQ=255)

                                                                           gATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGataa                                                            <  1:1132761/140‑1 (MQ=255)

                                                                           gATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGataa                                                            <  1:5534872/140‑1 (MQ=255)

                                                                           gATGCCGGTGAAGATGTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGataa                                                            <  2:8799128/140‑1 (MQ=255)

                                                                             tGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGataata                                                          <  1:4484622/140‑1 (MQ=255)

                                                                               ccGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATaataat                                                        <  2:9651425/140‑1 (MQ=255)

                                                                               ccGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATaataat                                                        <  2:6183474/140‑1 (MQ=255)

                                                                               ccGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATaataat                                                        >  2:3471956/1‑140 (MQ=255)

                                                                                  gTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAg                                                     >  2:4548122/1‑140 (MQ=255)

                                                                                   tGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGt                                                    <  1:2722373/140‑1 (MQ=255)

                                                                                      aGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGc                                                 <  1:2445229/140‑1 (MQ=255)

                                                                                              tggCTCTCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCAcc                                         <  1:7518747/138‑1 (MQ=255)

                                                                                                  tATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGcc                                     >  2:3870936/1‑140 (MQ=255)

                                                                                                    tCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCtt                                   >  2:3712426/1‑140 (MQ=255)

                                                                                                         aaGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAg                              <  2:5647434/140‑1 (MQ=255)

                                                                                                            cccGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCaa                           >  2:1007383/1‑140 (MQ=255)

                                                                                                            cccGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGGACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCaa                           <  1:4294977/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  2:4097235/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  1:5736462/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  1:6052349/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGGTGCGATCGGTGCCACGGTAACTGAAGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  2:6872394/140‑1 (MQ=255)

                                                                                                                cgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATACTCATCAGTGGCAGGTCACCCGCCTTGCCTGCAATAcc                       >  2:1293738/1‑140 (MQ=255)

                                                                                                                cgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAcc                       >  2:8997167/1‑140 (MQ=255)

                                                                                                                cgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAcc                       >  2:3385532/1‑140 (MQ=255)

                                                                                                                cgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAcc                       >  1:2116116/1‑140 (MQ=255)

                                                                                                                cgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAcc                       >  1:1855852/1‑140 (MQ=255)

                                                                                                                cgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAcc                       >  1:1706878/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATCATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  2:6627061/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        >  2:8096084/1‑126 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  1:7200441/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  1:7623167/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  1:847023/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  1:5077624/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGAACGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTATTACGGTGa          >  1:9797134/1‑140 (MQ=255)

                                                                                                                                  aaaaCAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGACGCCg     >  1:1348608/1‑140 (MQ=255)

ACGCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑G‑GACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGACGCCGGAT  >  NZ_CP025268/1146368‑1146639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`