BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,629,176	(T)_7→8	pseudogene (141/392 nt)	ydeI ←	YdeI family stress tolerance OB fold protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,629,169	1	.	T	96.7%	1012.1 / 20.8	271	pseudogene (148/392 nt)	ydeI	YdeI family stress tolerance OB fold protein
Reads supporting (aligned to +/- strand): ref base . (1/8); new base T (64/197); total (65/206)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

ACGGCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑T‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATG  >  NZ_CP025268/1629031‑1629302

   gCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑t‑t‑t‑t                                                                                                                                   >  1:2863617/1‑140 (MQ=255)

    ccGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑t‑t‑t‑tt                                                                                                                                  >  1:883452/1‑140 (MQ=255)

    ccGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑t‑t‑t‑tt                                                                                                                                  >  2:472606/1‑140 (MQ=255)

         ggAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑t‑t‑t‑tttttgc                                                                                                                             <  1:121098/140‑4 (MQ=255)

              aaCGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGcatcat                                                                                                                         <  2:1446492/140‑1 (MQ=255)

                cGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCg                                                                                                                       >  1:8789169/1‑140 (MQ=255)

                    aTTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTc                                                                                                                   >  2:46615/1‑140 (MQ=255)

                    aTTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTc                                                                                                                   <  1:7493368/140‑1 (MQ=255)

                     ttAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAAGCAACGGTCA‑T‑TGT‑TTTTGCATCATCGGGTcc                                                                                                                  >  1:6184700/1‑140 (MQ=255)

                         attCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGa                                                                                                              <  1:6626060/139‑1 (MQ=255)

                            cACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAg                                                                                                           <  1:1024407/140‑1 (MQ=255)

                             aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGc                                                                                                          >  2:1638555/1‑140 (MQ=255)

                             aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGc                                                                                                          >  1:3092275/1‑140 (MQ=255)

                              ccGCTCTTATCGCGCACAACGTAACGGTCCTCTCCAGTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCCACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGcc                                                                                                         <  2:3304193/140‑1 (MQ=255)

                              ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGcc                                                                                                         <  1:3812495/140‑1 (MQ=255)

                              ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGcc                                                                                                         <  2:8593631/140‑1 (MQ=255)

                              ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGcc                                                                                                         <  1:1783504/140‑1 (MQ=255)

                                gCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCAt                                                                                                       <  2:3421981/140‑1 (MQ=255)

                                 ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCAtc                                                                                                      <  1:8342255/140‑1 (MQ=255)

                                 ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCAtc                                                                                                      <  2:1506699/140‑1 (MQ=255)

                                  tctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCAtct                                                                                                     <  2:9427385/140‑1 (MQ=255)

                                   ctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCAtctt                                                                                                    <  1:4646277/140‑1 (MQ=255)

                                           gAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCa                                                                                            <  2:6282596/140‑1 (MQ=255)

                                            aaaaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCAt                                                                                           >  1:3518003/1‑140 (MQ=255)

                                            aaaaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCAt                                                                                           >  1:1583827/1‑140 (MQ=255)

                                                aCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAgg                                                                                       <  2:7917770/140‑1 (MQ=255)

                                                aCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAgg                                                                                       <  1:4040352/140‑1 (MQ=255)

                                                 cGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAggc                                                                                      <  2:2893673/140‑1 (MQ=255)

                                                 cGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCCTGCATGGTTTTTGCGAAATCAACGGTCA‑TAT‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAggc                                                                                      <  2:5105452/140‑1 (MQ=255)

                                                      cGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGcggcgg                                                                                 <  1:2522870/140‑1 (MQ=255)

                                                                  ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                     <  2:5173199/140‑1 (MQ=255)

                                                                  ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                     <  1:1293488/140‑1 (MQ=255)

                                                                  ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                     <  2:9222106/140‑1 (MQ=255)

                                                                  ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                     <  1:6499079/140‑1 (MQ=255)

                                                                  ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                     <  2:3496953/140‑1 (MQ=255)

                                                                  tggtgAGAAATCAAAGTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGGTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                     <  1:7227176/138‑1 (MQ=255)

                                                                   tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                    >  1:7722366/1‑140 (MQ=255)

                                                                   tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                    >  1:2683805/1‑140 (MQ=255)

                                                                   tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                    >  1:2602224/1‑140 (MQ=255)

                                                                   tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                    <  1:1607660/140‑1 (MQ=255)

                                                                     tgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTg                                                                  >  2:6526472/1‑140 (MQ=255)

                                                                     tgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGAGAGCCTTCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTg                                                                  >  2:6864016/1‑140 (MQ=255)

                                                                        gaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGttt                                                               >  1:7903890/1‑140 (MQ=255)

                                                                          aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTa                                                             <  2:2364225/140‑1 (MQ=255)

                                                                            tCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                           <  1:223343/140‑1 (MQ=255)

                                                                               aaTTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCg                                                        <  1:708256/140‑1 (MQ=255)

                                                                               aaTTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCg                                                        <  1:709966/140‑1 (MQ=255)

                                                                               aaTTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCg                                                        <  1:1926396/140‑1 (MQ=255)

                                                                                  tACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGccc                                                     <  1:5028855/140‑1 (MQ=255)

                                                                                   aCCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCt                                                    <  1:8902122/140‑1 (MQ=255)

                                                                                    ccGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTg                                                   <  2:5455663/140‑1 (MQ=255)

                                                                                     cgcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                     >  2:9636723/1‑121 (MQ=255)

                                                                                     cgcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                     <  1:9636723/121‑1 (MQ=255)

                                                                                     cgcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTgg                                                  >  2:8995366/1‑140 (MQ=255)

                                                                                     cgcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTgg                                                  <  2:9204304/140‑1 (MQ=255)

                                                                                      gcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGt                                                 >  2:1798921/1‑140 (MQ=255)

                                                                                       cgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTc                                                >  2:8259768/1‑140 (MQ=255)

                                                                                          aGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATc                                             <  2:5964718/140‑1 (MQ=255)

                                                                                          aGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATc                                             <  1:5712659/140‑1 (MQ=255)

                                                                                            tGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTg                                           >  1:6079965/1‑140 (MQ=255)

                                                                                             gAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑T‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                         <  1:875983/140‑1 (MQ=255)

                                                                                              aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                         >  2:6138201/1‑140 (MQ=255)

                                                                                              aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGGTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                         <  2:5566719/140‑1 (MQ=255)

                                                                                               aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCACCGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                        <  2:8692726/140‑1 (MQ=255)

                                                                                               aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                        <  1:3585740/140‑1 (MQ=255)

                                                                                               aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                        <  1:472606/140‑1 (MQ=255)

                                                                                               aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                        <  1:9534723/140‑1 (MQ=255)

                                                                                                aCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCaa                                       <  1:3877536/140‑1 (MQ=255)

                                                                                                aCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCaa                                       <  1:5459693/140‑1 (MQ=255)

                                                                                                    aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                                   <  1:6653547/140‑1 (MQ=255)

                                                                                                      gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATa                                 <  2:76026/140‑1 (MQ=255)

                                                                                                       cgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTCTTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATaa                                <  2:3752416/140‑1 (MQ=255)

                                                                                                       cgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATaa                                <  1:9588221/140‑1 (MQ=255)

                                                                                                         ccATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAgg                              <  1:959069/140‑1 (MQ=255)

                                                                                                            tCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATACGGCAt                           <  2:191639/140‑1 (MQ=255)

                                                                                                            tCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                           >  2:4834737/1‑140 (MQ=255)

                                                                                                            tCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                           <  1:5406981/140‑1 (MQ=255)

                                                                                                            tCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                           <  2:5793583/140‑1 (MQ=255)

                                                                                                            tCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                           <  1:3476198/140‑1 (MQ=255)

                                                                                                            tCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                           <  1:4368213/140‑1 (MQ=255)

                                                                                                            tCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                           <  1:3969452/140‑1 (MQ=255)

                                                                                                                 cATGGTTTTTGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACaa                      >  1:5007114/1‑140 (MQ=255)

                                                                                                                      tttttGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGTCGGTGGCGCTTCATACTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  2:4350494/140‑1 (MQ=255)

                                                                                                                      tttttGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  1:1404950/140‑1 (MQ=255)

                                                                                                                      tttttGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  2:8760956/140‑1 (MQ=255)

                                                                                                                      tttttGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  2:9058576/140‑1 (MQ=255)

                                                                                                                      tttttGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  1:5195457/140‑1 (MQ=255)

                                                                                                                      tttttGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  1:6159812/140‑1 (MQ=255)

                                                                                                                      tttttGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 >  1:6873184/1‑140 (MQ=255)

                                                                                                                      tttttGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  1:9239127/140‑1 (MQ=255)

                                                                                                                      tttttGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  2:1056878/140‑1 (MQ=255)

                                                                                                                      tttttGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGGTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  2:7188444/140‑1 (MQ=255)

                                                                                                                      tttttGAGAAATCAACGTTCATT‑T‑T‑TTTTGCATAATCGGGTCCTCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTTAACCGCCCTGGTCTTCTGCCAACGCATAAGGCATGACTAGaaaa                 <  2:8893036/140‑1 (MQ=255)

                                                                                                                      tttgtGCGAAATCCACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  2:7393570/140‑1 (MQ=255)

                                                                                                                        tttGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACt               <  2:4079090/140‑1 (MQ=255)

                                                                                                                        tgtGCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACt               <  2:675294/138‑1 (MQ=255)

                                                                                                                           gCGAAATCAACGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGc            <  2:5546591/140‑1 (MQ=255)

                                                                                                                                   aaCGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACaa    <  2:188183/140‑1 (MQ=255)

                                                                                                                                   aaCGGTCA‑T‑T‑TGTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACaa    <  2:8497604/140‑1 (MQ=255)

                                                                                                                                    aCGGTCATT‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAAt   <  2:3161295/140‑1 (MQ=255)

ACGGCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑T‑T‑T‑TTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATG  >  NZ_CP025268/1629031‑1629302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`