BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,660,178	+C	coding (314/351 nt)	ynfA ←	YnfA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,660,174	1	.	C	98.3%	1438.0 / 13.7	420	coding (318/351 nt)	ynfA	YnfA family protein
Reads supporting (aligned to +/- strand): ref base . (4/3); new base C (184/227); total (189/231)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 458 total aligned reads displayed.

CGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑CCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGC  >  NZ_CP025268/1660036‑1660308

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                        >  1:182733/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                        >  2:2391291/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATATTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                        >  2:750575/1‑140 (MQ=255)

 gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAAAAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑cc                                                                                                                                       >  1:8021036/1‑140 (MQ=255)

            gAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGca                                                                                                                            >  2:3076989/1‑140 (MQ=255)

             actGATCACAAAAAAAGCCGTATATTTTCTCTCAGAATGGTTGACAAAAGATGAAATAGAATACCTAATATCATCTTACACTTCATCTTAACTTATTTATAAAATGGATTTATGTTCATACGTACGCCCCC‑AGCCCGcac                                                                                                                           >  2:4427947/3‑139 (MQ=255)

             aGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGcaa                                                                                                                           <  1:1927458/140‑1 (MQ=255)

               tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGcaaca                                                                                                                         <  2:8479033/140‑1 (MQ=255)

               tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGcaaca                                                                                                                         <  1:402713/140‑1 (MQ=255)

                 aTCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAAt                                                                                                                       <  1:6115336/140‑1 (MQ=255)

                 aTCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTAATTGATACAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAAt                                                                                                                       >  2:1368069/1‑140 (MQ=255)

                    acaAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGAt                                                                                                                    <  1:4895213/140‑1 (MQ=255)

                      aaaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCa                                                                                                                  <  1:9726096/140‑1 (MQ=255)

                             ggCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGcc                                                                                                           >  1:9615850/1‑140 (MQ=255)

                               cGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGc                                                                                                         <  1:7098003/140‑1 (MQ=255)

                               cGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACGTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGc                                                                                                         <  2:6716412/140‑1 (MQ=255)

                                gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCa                                                                                                        >  2:5314998/1‑140 (MQ=255)

                                gTATATTTGCGCTGAGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTACCACTTATCTTATTGATAATATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACACTGATCAACAAGCCGCa                                                                                                        >  2:1799958/1‑140 (MQ=255)

                                  atatTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCaaa                                                                                                      >  1:2406056/1‑140 (MQ=255)

                                      ttGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAgcgc                                                                                                  <  1:5148289/140‑1 (MQ=255)

                                         cgcTGAGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                               >  2:9742487/1‑140 (MQ=255)

                                            tgtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCaa                                                                                            >  2:5784955/1‑140 (MQ=255)

                                             gtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAAc                                                                                           >  2:2222699/1‑140 (MQ=255)

                                              ttaaTGGTTTACAAAATATGAAATAGAATACATTTTGTCAGCTGACACTTCCTCTTATCTTATTGATTAATTGGATTTATGTTCCTACGTGCGACCCC‑TGCACGCTACAATGATAAACATGCCGCAAAGCGCAATCTACg                                                                                          <  2:2912896/138‑1 (MQ=255)

                                              tgAATGGTTGACAAAAGATTAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          <  2:6412720/140‑1 (MQ=255)

                                              tgAATGGTTGACAAAAGATGAAATAGACTACCTTTTGTCAGCTGACACTTCCTCGTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          <  2:3442015/140‑1 (MQ=255)

                                              tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          >  1:1009403/1‑140 (MQ=255)

                                              tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          >  2:5382479/1‑140 (MQ=255)

                                              tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGGTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          >  1:8933161/1‑140 (MQ=255)

                                              tgAATGGTTGACAAAAGATGAAATAGAAAACCTTTTGTCAGCTGACACTTCCTCATATCTTATTGATAAAAACGATTTATGTTCCTACGAGCGCCCCC‑AGCCCGCAACAAAGATCAACAAGCCGCAAAGCGCAATCAACg                                                                                          >  2:4597952/1‑140 (MQ=255)

                                                aaTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                                        <  1:7258967/140‑1 (MQ=255)

                                                 aTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                       <  2:1091388/140‑1 (MQ=255)

                                                      tGACTAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAAGGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  2:504146/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  2:5238368/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAAAGGATTTATGTTCCTACTTGCGCCCCC‑AGCCCGCAACAATTATCAAAATGCCGCAAAGCTCAAGCAACGCACCCGTc                                                                                  >  2:200651/1‑140 (MQ=255)

                                                        accacaGATGAATGAGAATACCTTTGGTCAGCTGACACTTGCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACTTGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                <  2:8078684/135‑1 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCACAGCGCAATCAACGCACCCGTCCa                                                                                >  2:7323762/1‑140 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:5940865/1‑140 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:1143074/1‑140 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                <  2:2725567/140‑1 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  2:6666442/1‑140 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:2912586/1‑140 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:3542858/1‑140 (MQ=255)

                                                          aaaaGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGt                                                                              <  1:3211266/140‑1 (MQ=255)

                                                                         aTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGtt                                                               >  1:768675/1‑140 (MQ=255)

                                                                          tACCTTTTGTCAGCTGACACTTCCTATTATATTATGGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAAAGATCAACATGCCGCAAAGAGCAATCAAAGCTCCCGTCCAGACATAACGAGGCAGttt                                                              >  2:6991919/1‑140 (MQ=255)

                                                                             cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCAc                                                           <  1:5795125/140‑1 (MQ=255)

                                                                             cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCAc                                                           >  1:4367265/1‑140 (MQ=255)

                                                                              ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          <  1:5284099/140‑1 (MQ=255)

                                                                              ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          <  2:7248288/140‑1 (MQ=255)

                                                                              atttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          <  1:5965034/139‑1 (MQ=255)

                                                                               tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                         >  1:4502288/1‑140 (MQ=255)

                                                                               tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                         >  1:6904394/1‑140 (MQ=255)

                                                                                ttGTCAGCTGACAATTCCTCTTATATTCTTGATAAAATGGATTTATGTTCCTACGTGCG‑CCCCAAGCCAGCAACAATGATCAACATTCCGAAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGcc                                                        <  2:1662858/140‑1 (MQ=255)

                                                                                  gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCAt                                                      >  1:1579675/1‑140 (MQ=255)

                                                                                  gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCAt                                                      >  1:855727/1‑140 (MQ=255)

                                                                                      gCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcac                                                  <  1:8387058/140‑1 (MQ=255)

                                                                                       cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCACTCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                                 >  2:7560639/1‑140 (MQ=255)

                                                                                       cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                                 >  1:2104219/1‑140 (MQ=255)

                                                                                           cacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgc                                             <  1:7454099/140‑1 (MQ=255)

                                                                                            acTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgcg                                            <  2:3952467/140‑1 (MQ=255)

                                                                                             cctCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACgcgc                                           >  2:9340531/3‑140 (MQ=255)

                                                                                             cTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACgcgc                                           >  2:3873278/1‑140 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:1305935/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  2:4259722/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:3948973/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:8198173/140‑1 (MQ=255)

                                                                                                 ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATa                                                                          >  1:5540002/1‑105 (MQ=255)

                                                                                                 ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATa                                                                          <  2:5540002/105‑1 (MQ=255)

                                                                                                 ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATa                                                                          >  1:6811516/1‑105 (MQ=255)

                                                                                                 ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGcc                                       >  1:5690422/1‑140 (MQ=255)

                                                                                                 ctcaaatctaatTGATAAAATTGATTTATTTTCCTACGTGCGCCCCC‑AGCCAGCAACAATGATCATCATAACGCAAAGCGCAATCAACGCCGCCGTCCAGTCATAAATCGACAGTTTCACGACATCCACAAAGCGCAGcc                                       >  2:8773138/6‑140 (MQ=255)

                                                                                                      atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                                  >  1:1997364/1‑140 (MQ=255)

                                                                                                      atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                                  >  2:3510573/1‑140 (MQ=255)

                                                                                                        cttatTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCaa                                >  2:6149492/1‑140 (MQ=255)

                                                                                                          tatTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                              <  2:3618323/140‑1 (MQ=255)

                                                                                                          tatTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                              <  1:2300949/140‑1 (MQ=255)

                                                                                                           atTGATAACATGGATTTATGTTCCTACGTGCGACCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  2:8637296/1‑140 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  1:8675092/1‑140 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  1:6220640/1‑140 (MQ=255)

                                                                                                            ttGATAAAATGGATTTATGTTCCAACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGATTCACGCCATCCACAACGCGCAGCCACATCAACGcc                            >  2:857409/1‑140 (MQ=255)

                                                                                                               aTAAAATTGATTTATGTTCCTAAGTGCGCCCCC‑ATCCCGCAAAAATGATCAACATGCCGCAAATCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACAGCAACGCCGTg                         <  2:9011020/140‑1 (MQ=255)

                                                                                                               aTAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                         <  1:2664380/140‑1 (MQ=255)

                                                                                                               aTAAAATGGATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                         <  1:8807355/140‑1 (MQ=255)

                                                                                                                       gATTTATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATa                 <  2:8351223/140‑1 (MQ=255)

                                                                                                                           tATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             <  1:4873929/140‑1 (MQ=255)

                                                                                                                           tATGTTCCTACGTGCGCCCCC‑AGCCCGCAACAAGGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             >  1:3455379/1‑140 (MQ=255)

                                                                                                                            aTGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGc            >  1:2569864/1‑140 (MQ=255)

                                                                                                                            aTGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGc            >  1:4643259/1‑140 (MQ=255)

                                                                                                                             tGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGcc           <  2:6348391/140‑1 (MQ=255)

                                                                                                                             tGTTCCTACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGcc           <  2:5873270/140‑1 (MQ=255)

                                                                                                                                 cctACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGccacca       >  1:908180/3‑140 (MQ=255)

                                                                                                                                  ctACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACACCGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCAt      <  1:995363/139‑1 (MQ=255)

                                                                                                                                  ctACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGTCATAAACGCCACCAt      >  2:4826345/2‑140 (MQ=255)

                                                                                                                                  ctACGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCAt      >  1:9417882/2‑140 (MQ=255)

                                                                                                                                    aCGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATaa    >  2:7749068/1‑140 (MQ=255)

                                                                                                                                     cGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCACCGCCGTGCAGACATAAACGCCACCATAAg   <  2:3263378/140‑1 (MQ=255)

                                                                                                                                     cGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAg   >  2:4069517/1‑140 (MQ=255)

                                                                                                                                     cGTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGCTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAg   <  1:657126/140‑1 (MQ=255)

                                                                                                                                      gTGCGCCCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGc  <  1:6050367/140‑1 (MQ=255)

CGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑CCCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGC  >  NZ_CP025268/1660036‑1660308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`