BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,873,836	(T)_5→6	pseudogene (67/1283 nt)	yeaH →	YeaH/YhbH family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,873,831	1	.	T	97.5%	1816.7 / 28.2	485	pseudogene (62/1283 nt)	yeaH	YeaH/YhbH family protein
Reads supporting (aligned to +/- strand): ref base . (5/7); new base T (300/170); total (306/179)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 561 total aligned reads displayed.

CAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑TT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATC  >  NZ_CP025268/1873692‑1873964

cAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑g                                                                                                                                          >  2:7171876/1‑140 (MQ=255)

 aaCGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑t                                                                                                                                        <  2:6469924/140‑1 (MQ=255)

     aCAGCGAACCGTGNGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACANAAGCATGGTGAATCGCCAGC‑G‑tt‑tt‑t                                                                                                                                  >  1:6252606/1‑140 (MQ=255)

     aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAATGCATGGTGAATCGCCAGC‑G‑tt‑tt‑t                                                                                                                                  >  1:8263647/1‑140 (MQ=255)

     aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tt‑t                                                                                                                                  <  1:5414912/140‑1 (MQ=255)

     aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tt‑t                                                                                                                                  >  2:5477551/1‑140 (MQ=255)

      cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tt‑tt                                                                                                                                 >  1:4529982/1‑139 (MQ=255)

      cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tt‑tt                                                                                                                                 >  2:3802088/1‑139 (MQ=255)

      cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tt‑tt                                                                                                                                 >  1:3220958/1‑139 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tt‑tta                                                                                                                                >  2:8980901/1‑138 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tt‑tta                                                                                                                                >  2:3550015/1‑138 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tt‑tta                                                                                                                                <  1:3731429/140‑3 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tt‑ttacg                                                                                                                              >  1:2978802/1‑136 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tt‑ttacg                                                                                                                              <  1:5045416/140‑5 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tt‑ttacg                                                                                                                              >  2:5543495/1‑136 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tt‑ttacg                                                                                                                              >  1:1466607/1‑136 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tt‑ttacg                                                                                                                              <  1:1209689/140‑5 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTGTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tt‑ttacg                                                                                                                              <  2:1123105/140‑5 (MQ=255)

                tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTtat                                                                                                                        >  2:9650834/1‑140 (MQ=255)

                     cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAgc                                                                                                                   <  1:5677466/140‑1 (MQ=255)

                     cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATGGACCGGCGTCTGAACGGCAACAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAgc                                                                                                                   <  2:5694811/140‑1 (MQ=255)

                      tGAGAAGCGGCAACCCAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATAGGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAgcgc                                                                                                                 <  2:6248111/140‑1 (MQ=255)

                        agaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCa                                                                                                                >  2:88998/1‑140 (MQ=255)

                        agaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCa                                                                                                                >  2:4393141/1‑140 (MQ=255)

                             cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAAACGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTa                                                                                                           >  2:1570974/1‑140 (MQ=255)

                               gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTaaa                                                                                                         >  1:2649210/1‑140 (MQ=255)

                               gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGATTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTaaa                                                                                                         <  2:6691131/140‑1 (MQ=255)

                                caacaaAGTCGTATCATACAGTTGGCAAATCTAGTACGGTGTGCATATGACCTGGTTTATTGACCGGAGTCTGCACGGCAAAAACAAAAGAATGTTGAATCGCCAGCTT‑TT‑TT‑TAAGCCTTTATACAGCGCAAATGaacc                                                                                                        <  2:5282538/140‑3 (MQ=255)

                                     caGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                                   <  1:5204906/140‑1 (MQ=255)

                                      aGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGa                                                                                                  <  1:9293941/140‑1 (MQ=255)

                                       ggCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGAt                                                                                                 >  1:8153634/1‑140 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  2:6921001/140‑1 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  2:3129558/140‑1 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  1:8082947/140‑1 (MQ=255)

                                                tccAGTTGGCAACTGTAGTACGGGGGGCATCTGACCTGGTTTATTGACCGGCGTCTGAACCGCAACAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGc                                                                                        <  2:7635226/138‑1 (MQ=255)

                                                tACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGc                                                                                        <  1:3802088/140‑1 (MQ=255)

                                                    gTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAtt                                                                                    >  1:2474426/1‑140 (MQ=255)

                                                     ttGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATta                                                                                   >  1:3059185/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  1:6070496/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  2:7331660/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  2:2709001/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  2:4704653/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGTCCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATTAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtgatc                                                                                >  2:4754835/1‑139 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCCTtcata                                                                                >  2:9103187/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                >  1:2166628/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                >  2:8419276/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                >  2:2220922/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTGATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                >  2:9500027/1‑140 (MQ=255)

                                                               tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTc                                                                         >  1:4236863/1‑140 (MQ=255)

                                                               tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTc                                                                         <  2:2068701/140‑1 (MQ=255)

                                                               tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTc                                                                         >  2:5843137/1‑140 (MQ=255)

                                                                    cGGGTGGAATATGACCTGGTTTATTGACCGGCGTCTGAACGTCAAAAACAAAATCATTGTGAATCGACAGA‑GTTT‑TT‑TACTCCGTTATAAATCGCAAATTAAACAGTCTATCTCCGATGACATTAATAAGCGTTCGGtga                                                                    <  2:3254542/140‑1 (MQ=255)

                                                                    cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGcc                                                                                       <  1:6346853/121‑1 (MQ=255)

                                                                    cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGcc                                                                                       >  2:6346853/1‑121 (MQ=255)

                                                                    cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                    <  1:6519385/140‑1 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgagtg                                                                 >  2:2354049/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  1:3430270/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  1:7276348/1‑140 (MQ=255)

                                                                        gggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactga                                                                >  2:7524078/1‑140 (MQ=255)

                                                                         ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                               >  2:2993675/1‑140 (MQ=255)

                                                                           cATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGt                                                             >  1:5851281/1‑140 (MQ=255)

                                                                           cATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGt                                                             >  2:7733107/1‑140 (MQ=255)

                                                                            atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTc                                                            >  2:1341691/1‑140 (MQ=255)

                                                                            atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTc                                                            >  1:8601189/1‑140 (MQ=255)

                                                                                   cTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGGTATAAAGCGCAAATTGTACAGTCGAGCTCCGAGGCCAGTATTAAGCGTGCGGTGACTGACGTCGTCAgcg                                                     >  2:5035974/1‑140 (MQ=255)

                                                                                    tGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcgg                                                    <  1:171413/140‑1 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   >  1:6553371/1‑140 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   >  1:8484809/1‑140 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   >  1:1645713/1‑140 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   >  1:968829/1‑140 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTGCGGTGACTGACGTCGACCgcggc                                                   >  2:8678504/1‑140 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAGGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  1:55138/140‑1 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  2:5900672/140‑1 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  1:4798642/140‑1 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  >  1:6943993/1‑140 (MQ=255)

                                                                                       tttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGa                                                 >  1:408909/1‑140 (MQ=255)

                                                                                         tATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAAt                                               >  2:3414171/1‑140 (MQ=255)

                                                                                           ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTCATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             >  1:950319/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  2:4095317/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  2:4991913/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  1:3961184/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        <  1:7064155/140‑1 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑tt‑tta                                                                                                                                <  1:7383306/51‑3 (MQ=255)

                                                                                                   cGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGAGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                                     >  2:3687062/1‑140 (MQ=255)

                                                                                                        gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                <  1:9076936/140‑1 (MQ=255)

                                                                                                          aCGTCAAAAACAAATTCATTCTGAATCGCCAGC‑T‑TT‑TTGTAAGCCTTAATAAAGCGCATATTAAACATTCGATCTCCTAGGCCAATAATAAGCGTTCGTTGACTGACGTCGACAGCGGAGAATCCGTATCCATTCCCACg                              <  2:8311047/140‑1 (MQ=255)

                                                                                                          aCGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCTCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGGATCCATTCCCACg                              <  2:3891072/140‑1 (MQ=255)

                                                                                                          aCGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GAAT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                              <  2:4759099/140‑1 (MQ=255)

                                                                                                            ggCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                            <  2:2548753/140‑1 (MQ=255)

                                                                                                             gCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGaa                           >  1:1045253/1‑140 (MQ=255)

                                                                                                              cAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAg                          >  1:7617099/1‑140 (MQ=255)

                                                                                                                      aaaGCATGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGc                  <  1:434979/140‑1 (MQ=255)

                                                                                                                            tGGTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAgc                                                                                                                   >  2:9368280/1‑37 (MQ=255)

                                                                                                                             ggTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                >  2:2878641/1‑119 (MQ=255)

                                                                                                                             ggTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                <  1:2878641/119‑1 (MQ=255)

                                                                                                                             ggTGAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGa           >  1:7799930/1‑140 (MQ=255)

                                                                                                                              gTGAATCGCCAGC‑G‑TTGTT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt          <  2:8572862/140‑1 (MQ=255)

                                                                                                                                gAATCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGt        <  2:1836994/140‑1 (MQ=255)

                                                                                                                                 aaTCGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGtt       >  1:879362/1‑140 (MQ=255)

                                                                                                                                    cGCCAGC‑GTTT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtca    >  2:7981377/1‑140 (MQ=255)

CAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑TT‑TT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATC  >  NZ_CP025268/1873692‑1873964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`