BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,913,492	+T	coding (88/144 nt)	mgrB ←	PhoP/PhoQ regulator MgrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,913,492	1	.	T	99.3%	2139.3 / ‑2.9	550	Q30K (CAG→AAG)	mgrB	PhoP/PhoQ regulator MgrB
Reads supporting (aligned to +/- strand): ref base . (1/2); major base T (282/261); minor base A (3/1); total (286/264)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 593 total aligned reads displayed.

TTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTG‑ATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGT  >  NZ_CP025268/1913353‑1913625

ttCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTg                                                                                                                                        >  1:8911919/1‑140 (MQ=255)

ttCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTg                                                                                                                                        <  1:978796/140‑1 (MQ=255)

ttCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTg                                                                                                                                        >  1:3053680/1‑140 (MQ=255)

ttCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGANATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGNACATCCTg                                                                                                                                        <  1:6324366/140‑1 (MQ=255)

  cGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCCCGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAGAATTGTACATCCTgtc                                                                                                                                      >  2:955475/1‑138 (MQ=255)

  cGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTgta                                                                                                                                      >  2:3508391/1‑138 (MQ=255)

  cGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTgta                                                                                                                                      <  1:8332992/140‑3 (MQ=255)

    ttCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGTCACAAATTCCGCTGAAAAATTGTACATCCTgtatc                                                                                                                                    >  2:7799289/1‑136 (MQ=255)

       aTTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGca                                                                                                                                 <  2:617118/140‑1 (MQ=255)

         ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACTGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               <  2:8991963/140‑1 (MQ=255)

         ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               <  2:1442361/140‑1 (MQ=255)

         ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               <  1:417958/140‑1 (MQ=255)

         ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               <  2:8889021/140‑1 (MQ=255)

           caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatc                                                                                                                             <  2:2540319/140‑1 (MQ=255)

           caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatc                                                                                                                             <  1:1506586/140‑1 (MQ=255)

            accaccTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatca                                                                                                                            <  2:4801508/140‑1 (MQ=255)

              caccTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatca                                                                                                                            >  2:691764/1‑138 (MQ=255)

              caccTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATg                                                                                                                          <  2:747024/140‑1 (MQ=255)

                 ctctGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTg                                                                                                                       >  1:2774721/1‑140 (MQ=255)

                  tctGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGa                                                                                                                      >  1:2226905/1‑140 (MQ=255)

                   ctGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATTATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGaa                                                                                                                     >  2:3887894/1‑140 (MQ=255)

                   ctGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGaa                                                                                                                     >  2:7019806/1‑140 (MQ=255)

                      aGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTgtatc                                                                                                                                    >  1:9641840/1‑118 (MQ=255)

                        tAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCt                                                                                                                <  2:342699/140‑1 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:6833101/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:7140707/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:5859637/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:2900149/1‑140 (MQ=255)

                             gcgcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGcc                                                                                                           >  1:4996455/1‑140 (MQ=255)

                             gcgcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGcc                                                                                                           >  1:3482466/1‑140 (MQ=255)

                               gcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCa                                                                                                         <  1:8200100/140‑1 (MQ=255)

                                cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  1:5093093/1‑140 (MQ=255)

                                cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  1:4731653/1‑140 (MQ=255)

                                        ccACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAgcagcac                                                                                                >  1:7390015/1‑139 (MQ=255)

                                        ccACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACAACGGGATAAACTGGTTAATGGCACAAATTCAGCTGAAAAATTGTACATCCAGTATCGAACATCATTTTGAATACCTGCGCCCAAAGCaccaa                                                                                                >  2:6468312/1‑140 (MQ=255)

                                         cACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaag                                                                                               <  2:4503086/140‑1 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               >  2:6796510/1‑106 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatca                                                                                                                            >  1:4577687/1‑109 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  2:4996358/1‑140 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  1:3792864/1‑140 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  2:3264162/1‑140 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACATGCGCCCAAAGCagcaagca                                                                                             >  2:5497673/1‑140 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTAAATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  1:8401199/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatc                                                                                                                             >  2:9295170/1‑106 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGcc                                                                                                           >  1:6904743/1‑124 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagcacg                                                                                           >  2:2954837/1‑138 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:1213751/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:9342872/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:2097344/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:3144378/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACAACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:1955329/1‑140 (MQ=255)

                                              ctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCaagcaagc                                                                                          >  1:9221352/1‑140 (MQ=255)

                                               ttgagaGTAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGTATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGcc                                                                                         <  2:6315349/138‑1 (MQ=255)

                                                   gagGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAaca                                                                                     <  2:2100453/140‑1 (MQ=255)

                                                      gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcacca                                                                                  >  2:4657431/1‑140 (MQ=255)

                                                      gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcacca                                                                                  >  1:8049558/1‑140 (MQ=255)

                                                      gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcacca                                                                                  >  2:5936042/1‑140 (MQ=255)

                                                      gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcacca                                                                                  >  1:5581799/1‑140 (MQ=255)

                                                      gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAACTCAGGTTGAAGACGTGCGCCCAAAGCAGCAAGCAAGCGAAcacca                                                                                  >  2:9174203/1‑140 (MQ=255)

                                                        agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                >  2:4189262/1‑140 (MQ=255)

                                                        agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                <  1:2730682/140‑1 (MQ=255)

                                                        agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                >  2:849701/1‑140 (MQ=255)

                                                                    gCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACg                                                                    >  1:3603826/1‑140 (MQ=255)

                                                                     cTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGa                                                                   <  2:2683807/140‑1 (MQ=255)

                                                                      tGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACATATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGAc                                                                  <  2:6251053/140‑1 (MQ=255)

                                                                        aaaaaaTGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGAccc                                                                >  2:5169609/1‑140 (MQ=255)

                                                                                           ggATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatc                                                                                                                             >  1:6723518/1‑60 (MQ=255)

                                                                                                aaCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACAGCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtct                                        <  2:7640374/140‑1 (MQ=255)

                                                                                                 aCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGcc                                                                                         <  2:9710660/90‑1 (MQ=255)

                                                                                                   tGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCg                                     <  1:8696113/140‑1 (MQ=255)

                                                                                                   tGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCg                                     <  1:4464150/140‑1 (MQ=255)

                                                                                                    ggTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                                    <  2:42371/140‑1 (MQ=255)

                                                                                                      ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTgta                                                                                                                                      >  1:6010732/1‑38 (MQ=255)

                                                                                                      ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  >  2:2182225/1‑140 (MQ=255)

                                                                                                      ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  >  1:1749088/1‑140 (MQ=255)

                                                                                                      ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  >  1:7922481/1‑140 (MQ=255)

                                                                                                      ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  <  1:6130160/140‑1 (MQ=255)

                                                                                                      ttAATGGCACAAATTCCGCTGAAAAATTGTACAACCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAAAATTTTAACTCCAATCACCGtta                                  >  2:7218435/1‑139 (MQ=255)

                                                                                                        aaTGCCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCa                                <  1:2577091/140‑1 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  1:1279000/140‑1 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  1:7714271/140‑1 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  1:5495857/140‑1 (MQ=255)

                                                                                                         aTGGCACAAATTCCGATGAAAAATTGTACCTCCGGTATCGCCCATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCTAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  1:9051944/140‑1 (MQ=255)

                                                                                                            gCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCaccta                            <  2:8743484/140‑1 (MQ=255)

                                                                                                             cacaAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctac                           >  1:1001199/1‑140 (MQ=255)

                                                                                                               caAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGACCGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCtcctacct                         >  1:1851839/1‑140 (MQ=255)

                                                                                                                 aaTTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTa                       <  2:8172562/140‑1 (MQ=255)

                                                                                                                  aTTCCGCTGAAATATTGTACATCCTGTATCGCACATCAGGTTGAATACCTGCGCCCAAAGCTGCAAGCAATCCAACAACTTGACAACCAGAACGACCGATCGAAACTTTTTCACTCCAATCTCCGTTTCAACTACCttct                      <  2:5685952/140‑3 (MQ=255)

                                                                                                                  aTTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTAt                      <  1:7589410/140‑1 (MQ=255)

                                                                                                                  aTTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTAt                      <  1:1587766/140‑1 (MQ=255)

                                                                                                                      cGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa                  <  1:6474934/140‑1 (MQ=255)

                                                                                                                       gCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCAt                 >  1:3239547/1‑140 (MQ=255)

                                                                                                                        cTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCAtt                <  1:7019806/140‑1 (MQ=255)

                                                                                                                           aaaaaTTGTACATCCTGTATCGCACATCATGTTGAAGACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTaaa             <  1:330420/140‑1 (MQ=255)

                                                                                                                               aTTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTATTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAg         <  2:2413533/140‑1 (MQ=255)

                                                                                                                                    aCATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGc    <  2:7980838/140‑1 (MQ=255)

                                                                                                                                    aCATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGc    <  2:1007016/140‑1 (MQ=255)

                                                                                                                                      aTCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGTACGTCCCATCGAAAATTTTTCACTCCTATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCgt  <  2:4037742/140‑1 (MQ=255)

                                                                                                                                      aTCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCgt  <  1:3953405/140‑1 (MQ=255)

                                                                                                                                      aTCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACACATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCCTGCgt  <  2:9410994/140‑1 (MQ=255)

TTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTG‑ATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGT  >  NZ_CP025268/1913353‑1913625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`