BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	247,295	+G	intergenic (+4/‑171)	yafL → / → rayT	C40 family peptidase/REP‑associated tyrosine transposase RayT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	247,294	1	.	G	99.0%	1399.1 / 4.6	409	intergenic (+3/‑172)	yafL/rayT	C40 family peptidase/REP‑associated tyrosine transposase RayT
Reads supporting (aligned to +/- strand): ref base . (2/2); new base G (168/237); total (170/239)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 475 total aligned reads displayed.

TGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑G‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAA  >  NZ_CP025268/247155‑247425

tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  2:4735484/1‑140 (MQ=255)

tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  1:9275112/1‑140 (MQ=255)

tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  1:9426938/1‑140 (MQ=255)

tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  1:3655122/1‑140 (MQ=255)

tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  2:6397449/1‑140 (MQ=255)

tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  1:2336243/1‑140 (MQ=255)

tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  2:7673835/1‑140 (MQ=255)

tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  2:8546789/1‑140 (MQ=255)

tgtAGTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  1:7022504/1‑140 (MQ=255)

tggaTTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGACCCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATGTTGTAGGAc                                                                                                                                       >  2:464371/4‑140 (MQ=255)

  tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑gg                                                                                                                                    >  1:7545184/1‑139 (MQ=255)

  tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑gg                                                                                                                                    >  1:4071056/1‑139 (MQ=255)

  tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑gg                                                                                                                                    >  1:6859288/1‑139 (MQ=255)

   aTTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑gga                                                                                                                                   >  1:3010358/1‑138 (MQ=255)

      tGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑ggataa                                                                                                                                <  1:9771759/140‑6 (MQ=255)

      tGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑ggataa                                                                                                                                <  1:4299299/140‑6 (MQ=255)

       gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAg                                                                                                                               >  1:3302162/1‑140 (MQ=255)

       gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAg                                                                                                                               >  1:854178/1‑140 (MQ=255)

       gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAg                                                                                                                               >  2:2775979/1‑140 (MQ=255)

       gggCGATGGGCAATTTATCGAGACGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAg                                                                                                                               >  1:5405355/1‑140 (MQ=255)

           gATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGt                                                                                                                           >  1:6579396/1‑140 (MQ=255)

           gATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGt                                                                                                                           >  1:4507776/1‑140 (MQ=255)

            aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                          <  1:5211828/140‑1 (MQ=255)

            aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                          >  1:7707590/1‑140 (MQ=255)

            aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                          >  2:5835861/1‑140 (MQ=255)

            aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGCAAAAGCCGATTAGCCGACCCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGAGTTTGTAGGACGG‑ATCAGGCGtg                                                                                                                          >  2:1112303/1‑139 (MQ=255)

               ggCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTAc                                                                                                                       >  1:9009118/1‑140 (MQ=255)

                gCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTAcg                                                                                                                      <  2:9275112/140‑1 (MQ=255)

                   aTTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTAcgccg                                                                                                                   <  2:368478/140‑1 (MQ=255)

                     ttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCa                                                                                                                 <  1:5257753/140‑1 (MQ=255)

                     ttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCa                                                                                                                 <  1:1600917/140‑1 (MQ=255)

                       aTCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTCGCCGAACCTTTCTGGCAGGACCC‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCTTc                                                                                                               >  2:748514/1‑140 (MQ=255)

                       aTCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATc                                                                                                               >  1:5475973/1‑140 (MQ=255)

                          gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  1:4559572/140‑1 (MQ=255)

                          gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  2:1603586/140‑1 (MQ=255)

                          gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  2:1026926/140‑1 (MQ=255)

                          gAGTAGCCAAGTACCGGAGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  2:3981214/140‑1 (MQ=38)

                             tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                         <  2:5266724/140‑1 (MQ=255)

                             tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                         <  1:6940088/140‑1 (MQ=255)

                              cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGt                                                                                                        >  1:4728042/1‑140 (MQ=255)

                              cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGt                                                                                                        >  1:9619473/1‑140 (MQ=255)

                               gCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCGTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGACGAGACGATGTTGTAGGACGG‑ATCAGGCGTTGACGCCGCATCCGGCAGtt                                                                                                       >  2:8951243/1‑140 (MQ=255)

                                ccACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTg                                                                                                      <  2:9503453/140‑1 (MQ=255)

                                ccACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTATCTGGCAGGACAA‑TATTTTGGGCGCGCGCAGGATTTTTACGGAAGAGACGATGATGTAGTAC‑GTATAAGGCGTTTAAGCCGCAACCGGCAGTTg                                                                                                      >  2:3713730/1‑140 (MQ=255)

                                  aCGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTa                                                                                                    <  1:2256816/140‑1 (MQ=255)

                                    gTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg                                                                                                  <  2:4926199/140‑1 (MQ=255)

                                    gTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg                                                                                                  <  1:790053/140‑1 (MQ=255)

                                      aCCGGCGAAACCCTTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                                <  2:7977659/140‑1 (MQ=255)

                                      aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                                <  2:2132887/140‑1 (MQ=255)

                                       ccGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAg                                                                                               <  2:333668/140‑1 (MQ=255)

                                         ggCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGt                                                                                             <  1:292057/140‑1 (MQ=255)

                                         ggCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGt                                                                                             <  2:5472051/140‑1 (MQ=255)

                                            gAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGcc                                                                                          <  1:5060790/140‑1 (MQ=255)

                                            gAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGcc                                                                                          <  2:833210/140‑1 (MQ=255)

                                            gAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGcc                                                                                          <  2:265790/140‑1 (MQ=255)

                                               aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGa                                                                                       >  2:3505938/1‑140 (MQ=255)

                                                 cATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                                                     <  1:2437554/140‑1 (MQ=255)

                                                 cATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                                                     <  1:8715160/140‑1 (MQ=255)

                                                    tCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGa                                                                                  >  2:1943968/1‑140 (MQ=255)

                                                        aTAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCt                                                                              >  1:1148346/1‑140 (MQ=255)

                                                        aTAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCt                                                                              >  2:1787693/1‑140 (MQ=255)

                                                           aGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:2674568/140‑1 (MQ=255)

                                                           aGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           >  1:4663701/1‑140 (MQ=255)

                                                           aGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:5915436/140‑1 (MQ=255)

                                                           aGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:3792269/140‑1 (MQ=255)

                                                           aGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:3556541/140‑1 (MQ=255)

                                                           aGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:218325/140‑1 (MQ=255)

                                                           aGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:1990539/140‑1 (MQ=255)

                                                           aGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:1861974/140‑1 (MQ=255)

                                                           aGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:1428615/140‑1 (MQ=255)

                                                            gCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TGTTGTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgcg                                                                          >  2:5647542/1‑140 (MQ=37)

                                                                aTTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTc                                                                      >  2:47897/1‑140 (MQ=255)

                                                                 ttAGCCGAACCTTTCTGGCAGGACCA‑TTTGTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCt                                                                     >  2:4443230/1‑140 (MQ=255)

                                                                  tAGCCGAACCTTTCTGGCCGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCtt                                                                    >  1:8096507/1‑140 (MQ=255)

                                                                  tAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCtt                                                                    >  2:7033043/1‑140 (MQ=255)

                                                                     ccGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                 <  2:7841024/140‑1 (MQ=255)

                                                                       gAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                               <  2:7188093/140‑1 (MQ=255)

                                                                       gAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                               >  2:1384484/1‑140 (MQ=255)

                                                                            tttCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTa                                                          <  2:9613953/140‑1 (MQ=255)

                                                                             ttCTGGCAGGACCATTTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTa                                                          >  2:2517226/1‑140 (MQ=25)

                                                                                   cAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGccc                                                   <  1:4895022/140‑1 (MQ=255)

                                                                                    aGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcg                                                  >  2:300714/1‑140 (MQ=255)

                                                                                     ggACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                                 >  1:8400030/1‑140 (MQ=255)

                                                                                     ggACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACCCCGCAGCCGGCAGTTGTACGCAGTTGCCTGATGCGACGCTGGCGCGTCTAAACATGCCTACGAGCCcgc                                                 >  2:4210649/1‑140 (MQ=25)

                                                                                                 tGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCg                                      <  2:9066817/140‑1 (MQ=255)

                                                                                                 tGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCg                                      <  1:7377923/140‑1 (MQ=255)

                                                                                                       cgcgcAGGATTTTGACGGAATAGACGATTTTGTAGGAC‑GTATAAGTCGTTTACGCCGCATCCGGCAGTTGTAACCAGGAGCCTTATGCGACGCTGGCTCGACATATAAATCCTACGAGCCAGCGAAAAAATGCGAGCCGc                                >  2:6608757/1‑140 (MQ=19)

                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                                >  2:2878230/1‑140 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                           <  2:2336243/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                           <  1:4180385/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                           <  2:7324995/140‑1 (MQ=255)

                                                                                                                 tttGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCATGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGata                      <  2:4056203/140‑1 (MQ=255)

                                                                                                                      cGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATaaaa                 <  1:1540305/140‑1 (MQ=255)

                                                                                                                        gAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTGGCGAGCCGCTTTCCCGATATAAaaca               >  1:6264448/1‑140 (MQ=255)

                                                                                                                        gAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGACCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca               >  2:6220794/1‑140 (MQ=255)

                                                                                                                            agaCGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCt           <  1:7187975/140‑1 (MQ=255)

                                                                                                                                   tttGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGcc    <  1:650921/140‑1 (MQ=255)

                                                                                                                                   tttGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATAGAAAACAACCTCAGTGcc    <  1:1816508/140‑1 (MQ=25)

                                                                                                                                     tGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                 <  2:388698/77‑1 (MQ=14)

                                                                                                                                     tGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                 >  1:1097971/1‑77 (MQ=14)

TGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCA‑TTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑G‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAA  >  NZ_CP025268/247155‑247425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`