BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,773,671	(C)_5→6	intergenic (+161/‑55)	yfjQ → / → yfjR	DUF932 domain‑containing protein/WYL domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,773,666	1	.	C	95.6%	1730.5 / 67.9	544	intergenic (+156/‑60)	yfjQ/yfjR	DUF932 domain‑containing protein/WYL domain‑containing protein
Reads supporting (aligned to +/- strand): ref base . (22/2); new base C (334/184); total (357/187)
Fisher's exact test for biased strand distribution p-value = 4.11e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 601 total aligned reads displayed.

CGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑C‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTG  >  NZ_CP025268/2773527‑2773801

cGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCg                                                                                                                                             <  2:1431919/140‑1 (MQ=255)

    gttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGGTGTTGCTGAGATATAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑c‑cc‑c                                                                                                                                      >  1:6484785/1‑140 (MQ=38)

    gttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTGACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑c‑cc‑c                                                                                                                                      >  1:718462/1‑140 (MQ=255)

     ttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑c‑cc‑cc                                                                                                                                     <  2:1549131/140‑1 (MQ=255)

     ttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTGATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑c‑cc‑cc                                                                                                                                     <  1:8648379/140‑1 (MQ=255)

     ttAATATCGGACAACACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑c‑cc‑cc                                                                                                                                     <  2:5001285/140‑1 (MQ=255)

       aaTATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCGTTTGCAGAACTCGAATATAGTTTCTGCGTTGGCCATACCCTGGCCG‑cgcgccg                                                                                                                                     >  1:76190/1‑134 (MQ=37)

       aaTATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTGTAACCCCCGTTCCATCTGGCTTTTGCAGAAATACAATCTGGTTTCTGCGTTGTCCAGCACCTGTCCG‑c‑cc‑ccct                                                                                                                                   >  2:9500241/1‑138 (MQ=37)

             ggACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTaa                                                                                                                             <  2:308320/140‑1 (MQ=255)

               acacCTCCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTCACCCCCGTTACATCTGGCTTTTGCAGACATAAATCATCGTTTCTGCGTTGTCCTTACCCTGTCCG‑C‑CCGCC‑TCTATAAAg                                                                                                                           >  2:8414897/1‑140 (MQ=255)

                caccacCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATATTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGt                                                                                                                          <  2:2567467/140‑1 (MQ=255)

                    accTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATc                                                                                                                      <  1:5875694/140‑1 (MQ=255)

                      cTGTCCGCATCGATATGTGATCGTGTGACTCAATACCCCTGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTATGCGTTGTACATACCCTGTACGCC‑CC‑CC‑TCTTTATAGTAATctc                                                                                                                    <  2:5136109/140‑3 (MQ=21)

                        gTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATGCCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCAcat                                                                                                                  <  2:2337652/140‑1 (MQ=255)

                        gTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCAcat                                                                                                                  <  1:9784404/140‑1 (MQ=255)

                         tCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑C‑CCGCC‑TCTTTAATGTCATCAcatc                                                                                                                 >  1:4242518/1‑140 (MQ=255)

                         tCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTGACATCTGGCTTTTGCAGAAATAAAAGATAGTTTCTGCGTTGTCCATTCCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCAcatc                                                                                                                 >  1:5153400/1‑140 (MQ=25)

                           cGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTACTCAcatcat                                                                                                               >  1:3701742/1‑140 (MQ=255)

                             cATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTTCATCTGGCTTTTGCAGATGTAAAAAATTGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAGGGAATCACATCAttg                                                                                                             >  2:111700/1‑139 (MQ=255)

                             cATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCAttt                                                                                                             >  1:5054165/1‑140 (MQ=255)

                             cATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTGTAATGTGATCAcatcatgt                                                                                                             >  1:1764115/1‑138 (MQ=255)

                             cATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTGTTGCAGAAATAAAATATAGTTTCTGCGTTGTCCATACCCTGTCCG‑C‑CC‑CCGGCTTTAATGTAAGCTCATCAgtt                                                                                                             >  2:7166302/1‑140 (MQ=255)

                                cGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAACTCAAAAATAGTTTCTGCGTTGTCCATAGCCTGTCCG‑CGCC‑CC‑TCTTTAAAGTAATCAGATCAtttgcg                                                                                                          >  2:7796212/1‑137 (MQ=25)

                                cGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTtca                                                                                                          <  1:2780356/140‑1 (MQ=255)

                                  cTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTtcagt                                                                                                        <  1:1506548/140‑1 (MQ=255)

                                         cTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAATAATAGTTTCTGCGTGGTCCATACCCTGTCCGCC‑CC‑CC‑GCGTTATAGGAATCACATCATTTTCAGTCAGTTaa                                                                                                 >  1:4123087/1‑140 (MQ=21)

                                         cTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                 <  2:4916980/140‑1 (MQ=255)

                                          tCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATAGCCTGTGCGCC‑CC‑CC‑TCTTTACAGTAATCACATCATTTTCAGTCAGttctc                                                                                                >  1:6368010/1‑137 (MQ=21)

                                          tCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTGAAc                                                                                                >  1:4507381/1‑140 (MQ=255)

                                          tCGTGTGCCTCAATCCCCCGGGTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATTCCCTGTCCG‑CGCC‑CC‑TGTTTTAAGTAATCTCATCATTTTCAGTCAGTTaag                                                                                                >  2:749292/1‑139 (MQ=21)

                                            gtgtGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACtt                                                                                              >  1:9478206/1‑140 (MQ=255)

                                            gtgtGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCGTTTGCAGAAATACAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑GCGTGCAAGTAAGCACATCATTTGCCGTCAGTTAACtt                                                                                              >  2:5927083/1‑140 (MQ=255)

                                             tgtgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAATAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTACTCACATCATTTTCAGTCAGTTAACttt                                                                                             >  1:7868817/1‑140 (MQ=255)

                                               tgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATGTGGCTTATGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATAGCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTGAGCCCATCATTTTCAGTCAGTTAACTTTcc                                                                                           >  1:9302375/1‑140 (MQ=18)

                                                 ccTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGACATAAATAATAGTTTCTGCGGTGTCCATACCCTGTCCGCC‑CC‑CC‑TCGTTAATGTAATCACATCATGTTCAGTCAGTTGACTTTCCTg                                                                                         >  2:9289135/1‑140 (MQ=18)

                                                 ccTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGACATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCGGTAAAGTACTCACATCATTTTCAGTCAGTTAACTTTCCTg                                                                                         >  1:1217551/1‑140 (MQ=18)

                                                 ccTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATCAATAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACCTAATTTTCAGTCAGGTTACTTTCCTg                                                                                         >  1:8032566/1‑140 (MQ=18)

                                                 ccTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTCACTTTCCTg                                                                                         >  1:8820156/1‑140 (MQ=255)

                                                 ccTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCGTTACAGTGATCACATCATTTTCAGACAGTGAGCTTGCCTg                                                                                         >  2:1783798/1‑140 (MQ=255)

                                                    cAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGgag                                                                                      >  2:2122652/1‑140 (MQ=255)

                                                      aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGgagta                                                                                    <  1:3294918/140‑3 (MQ=255)

                                                      aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGaa                                                                                    <  1:5495448/140‑1 (MQ=255)

                                                      aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGaa                                                                                    >  1:3935714/1‑140 (MQ=255)

                                                       tCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAt                                                                                   <  2:9687077/140‑1 (MQ=255)

                                                       tCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAt                                                                                   <  1:6721803/140‑1 (MQ=255)

                                                          cccGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTACGCC‑CC‑CC‑TCTTTAAAGTAATCAAATCATTTTCAGTCAGTTAACTGTCCTGGAGAAtctc                                                                                >  1:4348219/1‑140 (MQ=18)

                                                           ccGGTTATAGCTTTTAACCCCCGTTACATCTGGCGTTTGCAGAAATACAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGACAGTTAACTTTCCTGGAGAAtctct                                                                               >  2:4469270/1‑140 (MQ=18)

                                                            cGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctctc                                                                              >  2:2682787/1‑140 (MQ=255)

                                                              gTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCAt                                                                            <  2:8631745/140‑1 (MQ=255)

                                                                   aGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGacac                                                                       <  1:8468775/140‑1 (MQ=255)

                                                                        ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGaa                                                                                    <  1:1980032/122‑1 (MQ=255)

                                                                         tAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAg                                                                 >  2:3943524/1‑140 (MQ=255)

                                                                               ccGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcgcc                                                           >  1:8159228/1‑140 (MQ=255)

                                                                                cGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTTACTTTCCTGGAGAATCTCTCCTGACACAGGCAGAccgccg                                                          >  1:5351335/1‑140 (MQ=17)

                                                                                cGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcgccg                                                          >  1:1047060/1‑140 (MQ=255)

                                                                                      aTCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCAGACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGa                                                    <  2:6352703/140‑1 (MQ=255)

                                                                                          ggCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCCGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCgg                                                >  2:4262880/1‑140 (MQ=255)

                                                                                          ggCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCgg                                                >  2:6710536/1‑140 (MQ=255)

                                                                                          ggCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCgg                                                >  2:8786360/1‑140 (MQ=255)

                                                                                           gCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggc                                               >  2:3242918/1‑140 (MQ=255)

                                                                                            cTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggct                                              <  1:34794/140‑1 (MQ=255)

                                                                                               ttGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggctggc                                           >  2:2472850/1‑140 (MQ=255)

                                                                                                      aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcagg                                    <  1:6059473/140‑1 (MQ=255)

                                                                                                      aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcagg                                    <  1:7004684/140‑1 (MQ=255)

                                                                                                      aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcagg                                    <  1:3819878/140‑1 (MQ=255)

                                                                                                          aaaaaTAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTGAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcaggctgt                                <  2:2812036/140‑1 (MQ=255)

                                                                                                             aaTAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCATTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCAc                             <  2:8960742/140‑1 (MQ=255)

                                                                                                             aaTAGTTGCTGCGTGGTCCATACCCGGTCCGCC‑CC‑CC‑TCTTTAAAGTAAGCACATCATTTTCAGTCAGTGACCGTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCAc                             <  2:8169861/140‑1 (MQ=14)

                                                                                                               tAGTTTCTGCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑ACTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTg                           <  2:545688/140‑1 (MQ=255)

                                                                                                                       gCGTTGTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAg                   <  2:1968181/140‑1 (MQ=255)

                                                                                                                            gTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTc              >  1:2995376/1‑140 (MQ=255)

                                                                                                                            gTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTc              <  1:7251216/140‑1 (MQ=255)

                                                                                                                            gTCCATACCCTGTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTGTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTc              <  1:1407571/140‑1 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTATAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:5576457/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCAc                             >  2:122714/1‑114 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGc       >  1:9007259/1‑136 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCgggc   >  1:959647/1‑139 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:7321762/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:5780806/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   <  1:1257356/140‑1 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:4852370/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:9057855/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:4621476/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:1027453/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:7471159/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:2252781/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:248986/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:3369304/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:266336/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:344992/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:6535658/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:4044190/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:6210594/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:602686/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:4362242/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   <  2:5803457/140‑1 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:4664893/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:5324497/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:3105734/1‑140 (MQ=255)

                                                                                                                                       gTCCGCC‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGAAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:7980820/1‑140 (MQ=255)

CGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑C‑CC‑CC‑TCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTG  >  NZ_CP025268/2773527‑2773801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`