BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,449,793	+T	pseudogene (3757/3779 nt)	tamB →	autotransporter assembly complex protein TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,449,791	1	.	T	98.8%	2913.0 / 16.9	755	pseudogene (3755/3779 nt)	tamB	autotransporter assembly complex protein TamB
Reads supporting (aligned to +/- strand): ref base . (5/4); new base T (426/318); total (433/322)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 792 total aligned reads displayed.

GTCAGCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑T‑TGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTAT  >  NZ_CP025268/4449652‑4449925

gTCAGCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑a                                                                                                                                          >  2:5363135/1‑140 (MQ=255)

     cGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑t‑ttgc                                                                                                                                   >  2:4245833/1‑137 (MQ=255)

     cGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑t‑ttgc                                                                                                                                   >  1:2690651/1‑137 (MQ=255)

        cTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGctct                                                                                                                                 >  1:1535615/1‑140 (MQ=255)

           tGTAGTGCCAGGTCTGCACGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGAACAGGCACTGG‑ATT‑TGATCTATc                                                                                                                              >  2:5220691/1‑140 (MQ=255)

                tGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTc                                                                                                                         >  2:7053025/1‑140 (MQ=255)

                       tCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGtttt                                                                                                                  >  1:3358021/1‑140 (MQ=255)

                       tCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGtttt                                                                                                                  >  2:8516855/1‑140 (MQ=255)

                       tCTGCAAGTGAAATACGGCGTGGGTATAGTTGACTCTATAGCACCACGCACGTTACGTTATCGCCTGATGCCGAAGCTCTATCGGGAAGCCGTGTCTGGTGGAGACAAGGCACTGGCT‑T‑TGCTCTAGCAGTTCGAGtttg                                                                                                                  >  2:9384294/1‑139 (MQ=255)

                           cAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCa                                                                                                              >  2:7600440/1‑140 (MQ=255)

                           cAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCa                                                                                                              >  1:686123/1‑140 (MQ=255)

                              gTGAAATACGGCGTGGGTATAATTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATg                                                                                                           <  1:5263149/140‑1 (MQ=255)

                               tGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGc                                                                                                          >  1:4728325/1‑140 (MQ=255)

                                gAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCg                                                                                                         <  2:7260331/140‑1 (MQ=255)

                                   aTACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAat                                                                                                      >  2:946712/1‑140 (MQ=255)

                                         cGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCCCTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGt                                                                                                >  1:9872450/1‑140 (MQ=255)

                                         cGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGt                                                                                                >  1:9108045/1‑140 (MQ=255)

                                            gggTATATTTGACTCTAGAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTa                                                                                             <  2:6150755/140‑1 (MQ=255)

                                             ggTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTAc                                                                                            >  1:7597708/1‑140 (MQ=255)

                                              gTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACg                                                                                           <  2:2263110/140‑1 (MQ=255)

                                                 tatTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCa                                                                                        >  2:2801828/1‑140 (MQ=255)

                                                   tttGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTCGACCAGGCACTGG‑ATT‑TGCTCTCTCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGt                                                                                      >  2:8701578/1‑140 (MQ=255)

                                                   tttGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCAATATCTGGAATCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGATAATATTTGTCTACGGCAGt                                                                                      >  2:2002276/1‑140 (MQ=255)

                                                     tGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGttt                                                                                    <  2:746157/140‑1 (MQ=255)

                                                     tGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGttt                                                                                    <  2:3351492/140‑1 (MQ=255)

                                                       aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTAc                                                                                  >  2:3865866/1‑140 (MQ=255)

                                                       aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTAc                                                                                  >  2:1755276/1‑140 (MQ=255)

                                                       aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGATGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTAc                                                                                  >  2:8820247/1‑140 (MQ=255)

                                                        ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGATGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACg                                                                                 >  2:318378/1‑140 (MQ=255)

                                                        ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACg                                                                                 >  1:3143495/1‑140 (MQ=255)

                                                         tctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGc                                                                                >  2:7867088/1‑140 (MQ=255)

                                                          ctATAGCAACACTCACGTTACGTTATCGCCTGATGCCCACGCGATATCTGGAAGCCGTGTCTGGGGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCACTGCGAATTGTTGTCTACGGCAGTTTACGcc                                                                               <  2:5581676/140‑1 (MQ=255)

                                                             taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                            >  1:1977648/1‑140 (MQ=255)

                                                               gCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCacaaa                                                                          <  1:4402850/140‑1 (MQ=255)

                                                                   cacTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAg                                                                      >  1:7456294/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACACACAAGGc                                                                    >  2:4558669/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  1:6470923/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  1:6647784/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  1:7157507/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  1:9479688/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  1:9291168/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  1:4144907/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  1:5855330/1‑140 (MQ=255)

                                                                        acgttacgttATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑t‑t                                                                                                                                      >  2:1563665/1‑70 (MQ=255)

                                                                          gttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAg                                                               >  1:7012784/1‑140 (MQ=255)

                                                                           ttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                              <  1:5463430/140‑1 (MQ=255)

                                                                              cgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCAc                                                           >  2:3294627/1‑140 (MQ=255)

                                                                              cgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGATCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCAc                                                           >  1:3439451/1‑140 (MQ=255)

                                                                                   tCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                      <  1:4597386/140‑1 (MQ=255)

                                                                                   tCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                      <  1:2797269/140‑1 (MQ=255)

                                                                                      ccTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGcc                                                                               >  1:8211437/1‑112 (MQ=255)

                                                                                      ccTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAc                                                   <  2:1173312/140‑1 (MQ=255)

                                                                                       cTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAcc                                                  <  2:5829755/140‑1 (MQ=255)

                                                                                        tGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCa                                                 <  1:5102121/140‑1 (MQ=255)

                                                                                         gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                <  1:2228151/140‑1 (MQ=255)

                                                                                         gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                >  2:1326056/1‑140 (MQ=255)

                                                                                         gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                <  1:1140555/140‑1 (MQ=255)

                                                                                          aTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAGGACCAAt                                               >  2:9747871/1‑140 (MQ=255)

                                                                                                 aGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGt                                        >  2:2126502/1‑140 (MQ=255)

                                                                                                 aGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGt                                        >  1:5563890/1‑140 (MQ=255)

                                                                                                 aGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGt                                        >  1:5003542/1‑140 (MQ=255)

                                                                                                    tataTCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTAc                                     <  1:2273790/140‑1 (MQ=255)

                                                                                                       atCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTgg                                  >  1:1609122/1‑140 (MQ=255)

                                                                                                        tCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTggg                                 >  2:2498273/1‑140 (MQ=255)

                                                                                                        tCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTggg                                 >  2:4386127/1‑140 (MQ=255)

                                                                                                            gAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGGAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAt                             >  1:6146936/1‑140 (MQ=255)

                                                                                                            gAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAt                             >  2:2924673/1‑140 (MQ=255)

                                                                                                            gAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAt                             >  2:3821981/1‑140 (MQ=255)

                                                                                                            gAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAt                             >  2:9070479/1‑140 (MQ=255)

                                                                                                                ccGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                         <  2:9169367/140‑1 (MQ=255)

                                                                                                                ccGTGTCTGGTGTAGACCAGGCACTGG‑A‑TGTGCTCTATCAGTTCGAGTTTGAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAACCAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                         <  2:262444/140‑1 (MQ=255)

                                                                                                                   tgtCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTa                      >  2:8298981/1‑140 (MQ=255)

                                                                                                                    gtCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTCGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTAt                     <  1:9638115/140‑1 (MQ=255)

                                                                                                                       tGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGa                  <  2:4124620/140‑1 (MQ=255)

                                                                                                                         gtgtAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATa                <  1:7923385/140‑1 (MQ=255)

                                                                                                                          tgtAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATaa               >  1:1736742/1‑140 (MQ=255)

                                                                                                                          tgtAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATaa               >  1:8941541/1‑140 (MQ=255)

                                                                                                                            tAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACt             <  1:3973781/140‑1 (MQ=255)

                                                                                                                            tAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACt             <  1:8371060/140‑1 (MQ=255)

                                                                                                                            tAGACCAGGCACTGG‑ATT‑TGCTCTATCAGATCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACt             <  2:6606582/140‑1 (MQ=255)

                                                                                                                              gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc           <  1:254755/140‑1 (MQ=255)

                                                                                                                              gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc           <  2:3540552/140‑1 (MQ=255)

                                                                                                                              gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc           <  1:8729867/140‑1 (MQ=255)

                                                                                                                               aCCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAcc          >  1:6343621/1‑140 (MQ=255)

                                                                                                                               aCCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAcc          >  2:5039266/1‑140 (MQ=255)

                                                                                                                                ccAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa         <  2:648642/140‑1 (MQ=255)

                                                                                                                                ccAGGCACTGG‑ATT‑TGCTCTATCAGATCGAGTTTGAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa         <  1:2926129/140‑1 (MQ=255)

                                                                                                                                 cAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGTCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg        <  1:1408205/140‑1 (MQ=255)

                                                                                                                                 cAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg        <  1:4874935/140‑1 (MQ=255)

                                                                                                                                 cAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg        <  1:3859735/140‑1 (MQ=255)

                                                                                                                                 cAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg        <  2:7705457/140‑1 (MQ=255)

                                                                                                                                 cAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg        <  2:8072313/140‑1 (MQ=255)

                                                                                                                                    gCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg     >  1:9771983/1‑140 (MQ=255)

                                                                                                                                    gCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg     >  1:3584951/1‑140 (MQ=255)

                                                                                                                                    gCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg     >  2:4755151/1‑140 (MQ=255)

                                                                                                                                    gCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGACGATTTCAGTATCGATAACTACCAGTTg     >  2:6765518/1‑140 (MQ=255)

                                                                                                                                     cACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGt    <  1:4300659/140‑1 (MQ=255)

                                                                                                                                     cACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGt    <  1:7285649/140‑1 (MQ=255)

                                                                                                                                     cACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGt    <  1:7768144/140‑1 (MQ=255)

                                                                                                                                      aCTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGta   <  1:1009432/140‑1 (MQ=255)

GTCAGCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑T‑TGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTAT  >  NZ_CP025268/4449652‑4449925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`