Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,233,2250TA29.0% 221.1 / 37.2 149intergenic (+125/+248)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC
Reads supporting (aligned to +/- strand):  ref base T (46/59);  new base A (43/0);  total (89/60)
Fisher's exact test for biased strand distribution p-value = 1.41e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 149 total aligned reads displayed.

GATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTG  >  NZ_CP025268/2233088‑2233363
                                                                                                                                           |                                                                                                                                           
gatgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGGGGGGGG‑GGCCAAAcg                                                                                                                                           >  1:8107070/1‑140 (MQ=255)
 atgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATcgc                                                                                                                                          >  2:8462026/1‑140 (MQ=255)
 atgatATTTTAAAATGAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGGTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATcgc                                                                                                                                          >  2:9689761/1‑140 (MQ=255)
      atTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATcgccgccg                                                                                                                                     <  2:2048780/140‑1 (MQ=255)
               ttaattaaTGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCa                                                                                                                            >  2:8990819/1‑140 (MQ=255)
                           atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGC‑GGCCAAACGCCGCCCCCCCCAGCACCCCCCGGcgc‑t                                                                                                               >  1:8554154/1‑140 (MQ=37)
                           atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAACGCCGCCGCCCCCAGCACCCCCCCGctc‑t                                                                                                               >  2:2747220/1‑140 (MQ=255)
                            tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGAACCCCCGGGCTC‑Tg                                                                                                              >  2:954409/1‑140 (MQ=255)
                                gTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCCAAAGCCGCCCCCCCCAGCCCCCCCCGGCGC‑TGGCgc                                                                                                          >  2:2664139/1‑139 (MQ=25)
                                gTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCTGCCCCCTGCACCCCCGGGCTC‑TGGCGa                                                                                                          >  1:7937812/1‑140 (MQ=255)
                                 tCCGAAAATAACGAGAATATTTCAGTCGCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCCATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCgcc                                                                                                         >  2:7953538/1‑138 (MQ=255)
                                   cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCCGCACCCCCGGGCTC‑TGGCGAACa                                                                                                       >  1:163830/1‑140 (MQ=255)
                                     aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGGGGGGGG‑GGGCCAACGCCCCCCCCCCCCGCACCCCCCCGCGC‑AGGCGAACaaa                                                                                                     >  1:7425957/1‑140 (MQ=255)
                                     aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGG‑GGGCCAAAGCCGCCCCCCCCAGCACCCCCCGGCGC‑TGGGGGACaaa                                                                                                     >  2:1112170/1‑140 (MQ=25)
                                     aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCCGGCTC‑TGGCGAACaaa                                                                                                     >  2:4099327/1‑140 (MQ=255)
                                     aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTC‑TGGCGAACaaa                                                                                                     >  2:9444670/1‑140 (MQ=255)
                                          aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGG‑GCCCAAATGCC‑CCCCCCCCAGCACCCCCCCGCGC‑GGGCGGACaaaaaaaa                                                                                                >  1:1201665/1‑136 (MQ=25)
                                          aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGGGGGGGG‑GGGCCATAGCCCCCCCCCCCCGGCCCCCCCGGCCC‑TGGGGGACaaaaaaac                                                                                                >  1:3232722/1‑136 (MQ=25)
                                          aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGG‑GGCCAAACGCCGCCCCCCCCAGCACCCCCCCGCAC‑AGGCGGACCAAATagc                                                                                                >  1:8054214/1‑140 (MQ=25)
                                          aaCGAGAATATTTCAGTCTCTCAGCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTC‑TGGCGCCCAAAAAcgc                                                                                                >  2:1867434/1‑140 (MQ=25)
                                           aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGGCCAAAC‑CCCCCGCCCCCAGCACCCCCCGGCTC‑TGGCGAACAAAATcgcc                                                                                               >  2:4466982/1‑140 (MQ=21)
                                           aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAACGCCCCCCCCCCCATAACACCCCGGCAC‑AGGCGAACAAAAAcgac                                                                                               >  2:4467310/1‑138 (MQ=21)
                                                   atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCACGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGcgcg                                                                                       <  1:262449/140‑1 (MQ=255)
                                                     ttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGC‑GGCCAAACGCCGCCGCCCCCAGCACCCCCCGGCTC‑TGGCGAACAAAAAAGCcgcggcgcggg                                                                                     >  2:1475286/1‑139 (MQ=18)
                                                     ttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCAGCACCCCCCGGCTC‑TGGCGAAAAAAAACCCCCCGGCGCGGt                                                                                     >  2:2930092/1‑140 (MQ=18)
                                                     ttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAAAGCCCCCCCCCCCATCACCCCCCGGCAA‑AGGCCAAAAAAAAAGCCGCTGCGCggg                                                                                     >  2:5678576/1‑139 (MQ=18)
                                                     ttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCACGGGGC‑GGCCAAAAGCCGCCCCCCCCACCACCCCCGCGCAC‑AGGCCAACCAAAACGCCGCTGCGCGGt                                                                                     >  2:2274658/1‑140 (MQ=18)
                                                       cAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAACCCCGCCCCCCCCAGCACCCCCCGGCAC‑AGGCGAACAAAATCGCCGCGGCGCGGTGc                                                                                   >  2:5057598/1‑140 (MQ=18)
                                                         gTCTCTCCTCCTGTGGCGCTCCGGTCCTGTGCCTTGCTTCATATAATCACTGGCGCCCGGAGCGC‑GCCGGGGGC‑GGCCACGCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGccc                                                                                 <  2:4010806/140‑1 (MQ=18)
                                                         gTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCAC‑TGGCGAACAAAATCGCCGCTGCGCGGTGccc                                                                                 <  1:4920644/140‑1 (MQ=255)
                                                          tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAACGCCGCCGCCCCCAGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCAGCGCGGTGcccg                                                                                >  2:8895241/1‑139 (MQ=255)
                                                              tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc                                                                            >  2:7014670/1‑140 (MQ=255)
                                                               cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCt                                                                           <  2:3308495/140‑1 (MQ=255)
                                                               cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCt                                                                           <  2:6145057/140‑1 (MQ=255)
                                                               cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCt                                                                           <  2:3307058/140‑1 (MQ=255)
                                                                 tCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTa                                                                         <  1:7861887/140‑1 (MQ=255)
                                                                    tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAAAGCCCCCGCCCCCAGCACCCCCCGGCAC‑TGGCGAACAAAATCGCCGCTGCGCCGTGCCCACGGCTTATcc                                                                      >  2:8963005/1‑140 (MQ=18)
                                                                     gTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCACGGGGC‑GGCCCATCACCGCCCCCCCCAGCACCCCCCGGCTC‑TGGCGCACAAAAACGCCCCAGCGCGGTGCCCACGGCAAATccc                                                                     >  2:2937375/1‑140 (MQ=18)
                                                                      ttGCGCTCATGTCATGTGCATTGCTTCATATAATCACTGTCTCAAGGAGCGC‑GCAGGGGGC‑TGCCAAACGACGCCCCACCCATCAACCCCGGGGAC‑AGACGACACAAATCCCCGCTGAGCGGTGCCCTCGGCGTATCCCt                                                                    >  2:6525618/1‑140 (MQ=16)
                                                                        gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCCCTGCGCGGTGCCCTCGGCTTATCCCTTa                                                                  >  2:8006176/1‑140 (MQ=255)
                                                                        gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAACGCCGCCCCCCCCTGCACCCCCCGGCAC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCACGGCGTATCCCGTa                                                                  >  2:6725963/1‑140 (MQ=18)
                                                                        gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAAAGCCGCCCCCCCCAGCACCCCCCGGCAC‑AGGCGAACAAAATCGCCGCAGCGCGGTGCCCAAGGCATATACCATa                                                                  >  2:6518177/1‑140 (MQ=17)
                                                                           cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGC‑GGCCAATAGCCGCCGCCCCCAGCACCCCCGGGCTC‑AGGCGAAAAAAATCGCCGCTGCGCGGTGCCCTGGGCGTATCCCTTACgg                                                               >  2:8692573/1‑140 (MQ=18)
                                                                           cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCCATAGCCGCCCCCCCCAACACCCCCGGGGTC‑TGGCGAACAAAATCGCCGCAGAGCGGGGGCCGCGGCGTATACCTGACgg                                                               >  2:2920044/1‑140 (MQ=18)
                                                                            tCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGC‑GGCCACAACCCGCCCCCCCCAGCACCCCCCCGCGCATGGGGAACAAAA‑AGCCCCAGCGCGGTGCCCACCGCGTATACCATACAGc                                                              >  2:5819667/1‑140 (MQ=255)
                                                                                gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGC‑GGCCCAACGGCGCCGCCCCCGGCAACCCCCCGCTC‑TGGCGCACAAAATAGCCGCCGCGCGGTGGCCCCGGCGTATACCATCAGGCTAcc                                                          >  2:8155755/1‑140 (MQ=12)
                                                                                gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCAGCCCCCCCGGGCTC‑AGGCGAACAAAATCGCCGCTGCGCGGTGCCCACGGCTGATCCCATACGGCTAcc                                                          >  2:7972748/1‑140 (MQ=17)
                                                                                gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTG‑TGGCGAACAAAATCCCCCCTGCGCGGTGCCCACGGCTTATCCCTTAAGGCTAcc                                                          >  2:428103/1‑140 (MQ=17)
                                                                                      tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGC‑GGCCCATCGCCGCCGCCCCCAGCACCCCCGGGCTC‑TGGCGAACCAAATCGCCGCGGCGCGGTGCCCGCGGCGTAAACCCTACGGCTCCCGGGTCg                                                    >  2:6579217/1‑140 (MQ=14)
                                                                                      tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCACACGCCGCCCCCCCCGGCCCCCCCGGGGCC‑AGGCGCACCAAAACGCCGCGTCGCGGGGGCCGCGGCTTATCCCCTAAGGCGACCcgggag                                                    >  2:3633476/1‑137 (MQ=9)
                                                                                      tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAAAGCCGCCGCCCCCATCACCCCCCGGCTC‑TGGAGAACAAAATAGCCGCTGCGCGGTGCCCACCGCTTATCCCAAACGGCTACCGGGTCg                                                    >  2:717277/1‑140 (MQ=14)
                                                                                        cATGGCTTCATAAAAGCACGGGCGCATGGAGCGC‑GCCGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                  <  1:2190839/140‑1 (MQ=11)
                                                                                           tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGG‑GGGCCAAATCCCCCCCCCCCAGCACCCCCCGGCTA‑ATGCGCACAAAATAGCCCCCGCGCGGTGGCCCACGCGGATACCAGACGGCAAACGGGTCGGgggc                                               >  2:2514418/1‑140 (MQ=255)
                                                                                             cTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGGCAATCGCCGCCGCCCCCTGCACCCCCGGGGTC‑TGGCGCACAAAATCGCCGCTGCGCGGTGCCCTCCGCTTATCCCTTACGGCAACCGGGTCGGGCGCGa                                             >  2:9745036/1‑140 (MQ=14)
                                                                                                  tataATCACTGGCGCAAGGAGCGC‑GCCGGGGGG‑GGCCCAACGCCGCCCCCCCCCCCCCCCCCCGGCGC‑GGGGGAACAAAAAAGCCGCGGCGGCGGGCCCACCGCGTATCCCATACCGCAACCCGGTCGGgcgccggggac                                        >  2:3381359/1‑133 (MQ=2)
                                                                                                  tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                        <  1:300170/140‑1 (MQ=37)
                                                                                                  tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                        <  2:7405424/140‑1 (MQ=37)
                                                                                                  tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                        <  2:7204476/140‑1 (MQ=37)
                                                                                                  tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                        <  2:712306/140‑1 (MQ=37)
                                                                                                  tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                        <  1:2429778/140‑1 (MQ=37)
                                                                                                   ataATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑CGCCAAAACCCCCCACCCCCAGCACCCCCAGGCAC‑AGGCGAACAAAAAAGCCCCAGCCCCGTGCCCAAGGCTAAAACCCTACCGCTACCCGGGCGGGCGCGAGGTAAc                                       >  2:9413089/1‑140 (MQ=2)
                                                                                                    taATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                      <  2:9561341/140‑1 (MQ=35)
                                                                                                    taATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                      <  1:3849143/140‑1 (MQ=35)
                                                                                                    taATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                      <  2:4024790/140‑1 (MQ=35)
                                                                                                    taATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                      <  1:5706653/140‑1 (MQ=35)
                                                                                                     aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                     <  1:1503198/140‑1 (MQ=34)
                                                                                                     aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                     <  1:1503334/140‑1 (MQ=34)
                                                                                                       tCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAACCCCGCCCCCCCCAGCACCCCCCGGCTC‑AGGCGAACCAAAAACCCCCAGCGCCGTGCCCACCGCCTATACCATACGGCTACCCCGGAGGGCGCCAGGTAACaaac                                   >  2:9187930/1‑137 (MQ=2)
                                                                                                           tGGCGCAAGGAGCGC‑GCCGGGGGC‑GGCCCATCCCCCCCGCCCCCAGCACCCCCCGGCGC‑AGGCCAAACAAATCGCCGCTGCGCGGTGCCCACGGCTTATCCCTTACGGCTACCCGGGCGGGCCCGCGGTAACaaaccagt                               >  2:8434729/1‑133 (MQ=2)
                                                                                                           tGGCGCAAGGAGCGC‑GCAGGGGGG‑GGGCAATAGCCGCCCCCCCCAGCACCCCCCGGCTC‑TTGCGGACAAAAAAGCCGCGGCGCGGGGGCCCCGGCATATACCCTAAGGCGACCCGGGGGGGGGCGGGGTGACAACCCAGt                               >  2:5489908/1‑140 (MQ=2)
                                                                                                            ggCGCAAGGAGCGC‑GCAGGGGGG‑GGCCCAAAGCCCCCGCCCCCAGCACCCCCCGGCGC‑ATGCGAAAAAAAAAGCCCCAGCGCGGTGCCCACGGCTTATCACATAAAGCATCCCGGTCGGGGGCGAGGGTACATACCAGTa                              >  2:6397653/1‑140 (MQ=2)
                                                                                                                 aaGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAACTCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                         <  2:1653344/140‑1 (MQ=17)
                                                                                                                 aaGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                         <  1:3799966/140‑1 (MQ=32)
                                                                                                                 aaGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                         <  1:6752529/140‑1 (MQ=32)
                                                                                                                 aaGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                         <  2:7611827/140‑1 (MQ=32)
                                                                                                                  aGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAACATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgc                        <  2:207197/140‑1 (MQ=17)
                                                                                                                  aGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgc                        <  1:4347347/140‑1 (MQ=32)
                                                                                                                       cgc‑gcAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                   <  2:3417526/140‑1 (MQ=32)
                                                                                                                       cgc‑gcAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                   <  2:3918913/140‑1 (MQ=32)
                                                                                                                       cgc‑gcAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                   <  2:2280211/140‑1 (MQ=32)
                                                                                                                       cgc‑gcAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                   <  2:5513264/140‑1 (MQ=32)
                                                                                                                       cgc‑gcAGGGGGC‑GGCCAATCGCCGCCGCCCCCCGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                   <  2:163830/140‑1 (MQ=17)
                                                                                                                         c‑gcAGGGGGC‑GGCCCAACGCCCCCGCCCCCTGCACCCCCCGGCTC‑TGGCGAACCAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCCGGTCGGGCGCGAGGTAACATCCCAGGAAAACGCGCCCTCa                 >  2:8941187/1‑140 (MQ=11)
                                                                                                                            cAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc               <  2:3274980/140‑1 (MQ=32)
                                                                                                                               ggggC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCca            <  1:6082809/140‑1 (MQ=32)
                                                                                                                                 ggc‑ggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca          <  2:3770658/140‑1 (MQ=32)
                                                                                                                                 ggc‑ggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca          <  1:9825405/140‑1 (MQ=32)
                                                                                                                                  gc‑ggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACAt         <  2:3910561/140‑1 (MQ=32)
                                                                                                                                     ggcCAATCGCCGCCGCCCCCAGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCGTATCCCTTACGGCTACCCGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATcc       >  2:1876096/1‑140 (MQ=12)
                                                                                                                                      gcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa      <  1:2235252/140‑1 (MQ=32)
                                                                                                                                      gcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa      >  1:1801736/1‑140 (MQ=32)
                                                                                                                                      gcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa      >  2:6001545/1‑140 (MQ=32)
                                                                                                                                      gcCAATCGCCGCCGCCCCCAGCACCCCCGGGCAC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCGTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa      >  1:5985244/1‑140 (MQ=12)
                                                                                                                                       ccAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt     >  1:4100451/1‑140 (MQ=32)
                                                                                                                                       ccAATCGCCGCCGCCCCCAGCACCCCCCGGCTC‑TGGCGAACAAAATAGACGCTGCGCGGTGCCCTCGGCTTATCCCTGACGGCTACCGGGTCGGGCGCGAGGTCACCTCCCTGTAAATAGCGCCCTCAGCCCACATCCAt     >  1:9146468/1‑140 (MQ=11)
                                                                                                                                          aTCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTCACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg  >  1:1368734/1‑140 (MQ=17)
                                                                                                                                          aTCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg  >  1:2155493/1‑140 (MQ=32)
                                                                                                                                          aTCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg  >  1:6900865/1‑140 (MQ=32)
                                                                                                                                          aTCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg  >  1:200183/1‑140 (MQ=32)
                                                                                                                                          aTCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg  >  1:1368727/1‑140 (MQ=32)
                                                                                                                                           |                                                                                                                                           
GATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTG  >  NZ_CP025268/2233088‑2233363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑