Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,233,2390TA32.7% 244.4 / 50.6 161intergenic (+139/+234)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC
Reads supporting (aligned to +/- strand):  ref base T (36/65);  new base A (49/0);  total (95/66)
Fisher's exact test for biased strand distribution p-value = 1.31e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 164 total aligned reads displayed.

TTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTT  >  NZ_CP025268/2233103‑2233378
                                                                                                                                         |                                                                                                                                            
ttaattaaTGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCa                                                                                                                                           >  2:8990819/1‑140 (MQ=255)
            atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAAACGCCGCCCCCCCCAGCACCCCCCGGcgc‑t                                                                                                                              >  1:8554154/1‑140 (MQ=37)
            atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAAACGCCCCCGCCCCCACCACCCCCCGGcgc‑t                                                                                                                              >  1:7924936/1‑140 (MQ=37)
            atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCAGCACCCCCCCGctc‑t                                                                                                                              >  2:2747220/1‑140 (MQ=255)
             tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGAACCCCCGGGCTC‑Tg                                                                                                                             >  2:954409/1‑140 (MQ=255)
                  tCCGAAAATAACGAGAATATTTCAGTCGCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCgcc                                                                                                                        >  2:7953538/1‑138 (MQ=255)
                    cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCCGCACCCCCGGGCTC‑TGGCGAACa                                                                                                                      >  1:163830/1‑140 (MQ=255)
                      aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGGGGCCCAACGCCGCCCCCCCCACCACCCCCCGGCGC‑AGGCGGACaaa                                                                                                                    >  1:3918913/1‑140 (MQ=25)
                      aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGGCCAAAGCCGCCCCCCCCAGCACCCCCCGGCGC‑TGGGGGACaaa                                                                                                                    >  2:1112170/1‑140 (MQ=25)
                      aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTC‑TGGCGAACaaa                                                                                                                    >  2:9444670/1‑140 (MQ=255)
                           aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGCCCAAATGCC‑CCCCCCCCAGCACCCCCCCGCGC‑GGGCGGACaaaaaaaa                                                                                                               >  1:1201665/1‑136 (MQ=25)
                           aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGGGGGGGCGGCCAATCGCCGCCCCCCCCAGCACCCCCCCGCGC‑AGGCGCACAAAAAcgc                                                                                                               >  1:870258/1‑140 (MQ=25)
                           aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGGGGCCAAACGCCGCCCCCCCCAGCACCCCCCCGCAC‑AGGCGGACCAAATagc                                                                                                               >  1:8054214/1‑140 (MQ=25)
                           aaCGAGAATATTTCAGTCTCTCAGCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTC‑TGGCGCCCAAAAAcgc                                                                                                               >  2:1867434/1‑140 (MQ=25)
                            aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCCCCCCCCCCATAACACCCCGGCAC‑AGGCGAACAAAAAcgac                                                                                                              >  2:4467310/1‑138 (MQ=21)
                             cGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATcgccg                                                                                                             <  2:2960242/140‑1 (MQ=255)
                                    atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCACGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGcgcg                                                                                                      <  1:262449/140‑1 (MQ=255)
                                      ttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCACGGGGCGGCCAAAAGCCGCCCCCCCCACCACCCCCGCGCAC‑AGGCCAACCAAAACGCCGCTGCGCGGt                                                                                                    >  2:2274658/1‑140 (MQ=18)
                                       tCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTg                                                                                                   <  2:8560001/140‑1 (MQ=255)
                                        cAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACCCCGCCCCCCCCAGCACCCCCCGGCAC‑AGGCGAACAAAATCGCCGCGGCGCGGTGc                                                                                                  >  2:5057598/1‑140 (MQ=18)
                                          gTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCAC‑TGGCGAACAAAATCGCCGCTGCGCGGTGccc                                                                                                <  1:4920644/140‑1 (MQ=255)
                                           tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCAGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCAGCGCGGTGcccg                                                                                               >  2:8895241/1‑139 (MQ=255)
                                               tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc                                                                                           >  2:7014670/1‑140 (MQ=255)
                                                cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCt                                                                                          <  2:3307058/140‑1 (MQ=255)
                                                  tCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTa                                                                                        <  1:7861887/140‑1 (MQ=255)
                                                     tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCCCCGCCCCCAGCACCCCCCGGCAC‑TGGCGAACAAAATCGCCGCTGCGCCGTGCCCACGGCTTATcc                                                                                     >  2:8963005/1‑140 (MQ=18)
                                                      gTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCACGGGGCGGCCCATCACCGCCCCCCCCAGCACCCCCCGGCTC‑TGGCGCACAAAAACGCCCCAGCGCGGTGCCCACGGCAAATccc                                                                                    >  2:2937375/1‑140 (MQ=18)
                                                       ttGCGCTCATGTCATGTGCATTGCTTCATATAATCACTGTCTCAAGGAGCGC‑GCAGGGGGCTGCCAAACGACGCCCCACCCATCAACCCCGGGGAC‑AGACGACACAAATCCCCGCTGAGCGGTGCCCTCGGCGTATCCCt                                                                                   >  2:6525618/1‑140 (MQ=16)
                                                         gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGCGGCCAAAAGCCGCCGCCCCCAGCACCCCCCGGCAA‑TTGCGAACAAAATAGCCCCTGAGCGGTGGCCTCAGCTAAtaacata                                                                                 >  2:2678000/1‑134 (MQ=17)
                                                         gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCCCCCCCTGCACCCCCCGGCAC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCACGGCGTATCCCGTa                                                                                 >  2:6725963/1‑140 (MQ=18)
                                                             tCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGCGGCCACAACCCGCCCCCCCCAGCACCCCCCCGCGCATGGGGAACAAAA‑AGCCCCAGCGCGGTGCCCACCGCGTATACCATACAGc                                                                             >  2:5819667/1‑140 (MQ=255)
                                                                 gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGCGGCCCAACGGCGCCGCCCCCGGCAACCCCCCGCTC‑TGGCGCACAAAATAGCCGCCGCGCGGTGGCCCCGGCGTATACCATCAGGCTAcc                                                                         >  2:8155755/1‑140 (MQ=12)
                                                                 gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTG‑TGGCGAACAAAATCCCCCCTGCGCGGTGCCCACGGCTTATCCCTTAAGGCTAcc                                                                         >  2:428103/1‑140 (MQ=17)
                                                                 gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGACCACAATAGCCCCTGCGCGGTGCCCACGGCGGATCCCGGTCGGCTAcc                                                                         >  2:4084308/1‑140 (MQ=17)
                                                                       tgCCTTGCTTCATATACTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCCCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCAGCGCGGTGCCCTCGGCTTATCCCGTACGGCTCCCGGGTCg                                                                   >  2:7842502/1‑140 (MQ=14)
                                                                       tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCCATCGCCGCCGCCCCCAGCACCCCCGGGCTC‑TGGCGAACCAAATCGCCGCGGCGCGGTGCCCGCGGCGTAAACCCTACGGCTCCCGGGTCg                                                                   >  2:6579217/1‑140 (MQ=14)
                                                                       tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGGCAAAAGCCCCCGCCCCCAGCACCCCCCGGCAC‑TGGCCAACAAAAAACCCCCGTCCCGGTGGCCACGGCATAAACCTTACGGCAACCCGGtag                                                                   >  2:3784613/1‑138 (MQ=9)
                                                                       tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCACACGCCGCCCCCCCCGGCCCCCCCGGGGCC‑AGGCGCACCAAAACGCCGCGTCGCGGGGGCCGCGGCTTATCCCCTAAGGCGACCcgggag                                                                   >  2:3633476/1‑137 (MQ=9)
                                                                       tgCATTGCTTCATATAATCACGGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAACGCCCCCCCCCCCGGCACCCCCCGGCTA‑TGGCGCACAAAAACCCCGCTGCGCGGTGCCCGCGGCGTATCCCTTACGGCAACCCGGTCg                                                                   >  2:3986007/1‑140 (MQ=14)
                                                                         cATTGCTTCATATAATCACTGGCGCACGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCGTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                                 <  1:7237650/140‑1 (MQ=255)
                                                                         cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                                 <  1:2190557/140‑1 (MQ=255)
                                                                            tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGGGGGCCAAATCCCCCCCCCCCAGCACCCCCCGGCTA‑ATGCGCACAAAATAGCCCCCGCGCGGTGGCCCACGCGGATACCAGACGGCAAACGGGTCGGgggc                                                              >  2:2514418/1‑140 (MQ=255)
                                                                              cTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGGCAATCGCCGCCGCCCCCTGCACCCCCGGGGTC‑TGGCGCACAAAATCGCCGCTGCGCGGTGCCCTCCGCTTATCCCTTACGGCAACCGGGTCGGGCGCGa                                                            >  2:9745036/1‑140 (MQ=14)
                                                                                tCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAgg                                                          <  2:4289427/140‑1 (MQ=40)
                                                                                   tataATCACTGGCGCAAGGAGCGC‑GCCGGGGGGGGCCCAACGCCGCCCCCCCCCCCCCCCCCCGGCGC‑GGGGGAACAAAAAAGCCGCGGCGGCGGGCCCACCGCGTATCCCATACCGCAACCCGGTCGGgcgccggggac                                                       >  2:3381359/1‑133 (MQ=2)
                                                                                   tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                       <  1:2429778/140‑1 (MQ=37)
                                                                                   tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                       <  2:7204476/140‑1 (MQ=37)
                                                                                   tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                       <  1:300170/140‑1 (MQ=37)
                                                                                   tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                       <  1:1401748/140‑1 (MQ=37)
                                                                                     taATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                                     <  1:3849143/140‑1 (MQ=35)
                                                                                     taATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                                     <  2:9561341/140‑1 (MQ=35)
                                                                                     taATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                                     <  2:4024790/140‑1 (MQ=35)
                                                                                      tgccACTGGCGCAAGGAGCGC‑GCATGGGGCGGCCCAACGCCGCCGCCCCCTGCACCCCCGGGCTC‑AGGCGAACAAAATCGCCGCTGCGCGGGGCCCACGGCGTATCCCTTACCGCGACCGGGGAGGGCGCCAGGTaaaaa                                                    >  2:2923987/4‑137 (MQ=1)
                                                                                      tgccACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCCCCCCCGGCACCCCCCGGCTC‑TGGCGAACAAAAAAGCCCCAGCGCGGTGCCCACGGCTTAAACCCTACGGCAACCCGGGCGGGCGCGAGGTAACAt                                                    >  2:6500019/4‑140 (MQ=1)
                                                                                      aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                                    <  1:1503334/140‑1 (MQ=34)
                                                                                      aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCCCCGCCCCCACCACCCCCCCGCAC‑TGGCGAAAAAAATAGCCCCAGCGCCGGGCCCACGGCCTTTCACATACGGCCACCCGGTACGGCGCCACGGAACaa                                                    >  2:7289687/1‑139 (MQ=2)
                                                                                       aTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATc                                                   <  1:3495870/140‑1 (MQ=33)
                                                                                        tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAAACCCCCCCCCCCCCGCCCCCCCCGGCGC‑GGGCGCACAAAAAAGCCCCTGCGCGGGGCCCCCCGCGTATACCATAAGGCGACCCGGGCGGGCGCGCGGGaaaaacc                                                  >  2:1503334/1‑135 (MQ=2)
                                                                                            tGGCGCAAGGAGCGC‑GCCGGGGGCGGCCCATCCCCCCCGCCCCCAGCACCCCCCGGCGC‑AGGCCAAACAAATCGCCGCTGCGCGGTGCCCACGGCTTATCCCTTACGGCTACCCGGGCGGGCCCGCGGTAACaaaccagt                                              >  2:8434729/1‑133 (MQ=2)
                                                                                            tGGCGCAAGGAGCGC‑GCAGGGGGGGGGCAATAGCCGCCCCCCCCAGCACCCCCCGGCTC‑TTGCGGACAAAAAAGCCGCGGCGCGGGGGCCCCGGCATATACCCTAAGGCGACCCGGGGGGGGGCGGGGTGACAACCCAGt                                              >  2:5489908/1‑140 (MQ=2)
                                                                                            tGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCGGCACCCCCCGGCTC‑TGGCGAACAAAATAGCCGCTGCGCGGTGCCCACGGCGTATACCGTACGGCTAACGGGTCGGGCGCGAGGTAACAACCCAGt                                              >  2:7999222/1‑140 (MQ=11)
                                                                                             ggCGCAAGGAGCGC‑GCAGGGGGGGGCCCAAAGCCCCCGCCCCCAGCACCCCCCGGCGC‑ATGCGAAAAAAAAAGCCCCAGCGCGGTGCCCACGGCTTATCACATAAAGCATCCCGGTCGGGGGCGAGGGTACATACCAGTa                                             >  2:6397653/1‑140 (MQ=2)
                                                                                                  aaGGAGCGC‑GCAGGGGGCGGCCACTCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGGGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCAGTAACAcg                                        <  2:1653381/140‑1 (MQ=255)
                                                                                                  aaGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                                        <  2:7611827/140‑1 (MQ=32)
                                                                                                   aGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAACATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgc                                       <  2:207197/140‑1 (MQ=17)
                                                                                                   aGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgc                                       <  1:4347347/140‑1 (MQ=32)
                                                                                                      aGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGcc                                    <  2:6112969/140‑1 (MQ=32)
                                                                                                        cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                  <  2:5513264/140‑1 (MQ=32)
                                                                                                        cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                  <  2:2280211/140‑1 (MQ=32)
                                                                                                        cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                  <  2:3417526/140‑1 (MQ=32)
                                                                                                        cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCCGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                  <  2:163830/140‑1 (MQ=17)
                                                                                                          c‑gcAGGGGGCGGCCCAACGCCCCCGCCCCCTGCACCCCCCGGCTC‑TGGCGAACCAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCCGGTCGGGCGCGAGGTAACATCCCAGGAAAACGCGCCCTCa                                >  2:8941187/1‑140 (MQ=11)
                                                                                                             cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                              <  2:3274980/140‑1 (MQ=32)
                                                                                                                ggggCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCca                           <  1:6082809/140‑1 (MQ=32)
                                                                                                                  ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                         <  2:576749/140‑1 (MQ=32)
                                                                                                                  ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                         <  1:9825405/140‑1 (MQ=32)
                                                                                                                   gcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACAt                        <  2:3910561/140‑1 (MQ=32)
                                                                                                                     ggcCAATCGCCGCCGCCCCCAGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCGTATCCCTTACGGCTACCCGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATcc                      >  2:1876096/1‑140 (MQ=12)
                                                                                                                     ggcCAAACGCCGCCGCCCCCAGCACCCCCGGGCTC‑TGGCGAAAAAAAACGCCGCTGCGCGGTGCCCACGGCTTATACCTTACGGCTACCCGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCACAGCCCACAAcc                      >  2:7160591/1‑140 (MQ=11)
                                                                                                                      gcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa                     >  1:1801736/1‑140 (MQ=32)
                                                                                                                      gcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa                     <  1:2235252/140‑1 (MQ=32)
                                                                                                                      gcCAATCGCCGCCGCCCCCAGCACCCCCGGGCAC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCGTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa                     >  1:5985244/1‑140 (MQ=12)
                                                                                                                       ccAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAG‑CCACATCCAtg                   <  1:6694861/140‑1 (MQ=17)
                                                                                                                        cAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtg                   <  2:9213740/140‑1 (MQ=32)
                                                                                                                          aTCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTCACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                 >  1:1368734/1‑140 (MQ=17)
                                                                                                                          aTCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                 >  1:6900865/1‑140 (MQ=32)
                                                                                                                          aTCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                 >  1:200183/1‑140 (MQ=32)
                                                                                                                            cgccgccgcCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg               >  1:9431202/1‑140 (MQ=32)
                                                                                                                            cgccgccgcCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg               >  1:5851307/1‑140 (MQ=32)
                                                                                                                            cgccgccgcCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg               <  2:1496146/140‑1 (MQ=32)
                                                                                                                            cgccgccgcCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg               <  1:2664139/140‑1 (MQ=32)
                                                                                                                              ccgccgccCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCt             >  1:9182035/1‑140 (MQ=32)
                                                                                                                               cgccgccCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTg            >  1:4949663/1‑140 (MQ=32)
                                                                                                                                 ccgccCCCTGCACCCACGGGCTC‑TGGCGAACAAAATCGCCGCTGCTCGGTGCCCTCGGCGTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGATGcc          >  1:2165079/1‑140 (MQ=255)
                                                                                                                                  cgccCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGccc         <  2:3313862/140‑1 (MQ=32)
                                                                                                                                   gccCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcccc        <  2:8054214/140‑1 (MQ=32)
                                                                                                                                   gccCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcccc        <  2:6860066/140‑1 (MQ=32)
                                                                                                                                      cccTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCATTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGGGGGCTGCCCCGGc     <  2:1514210/140‑1 (MQ=17)
                                                                                                                                         tGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCtt  <  1:11219/140‑1 (MQ=32)
                                                                                                                                         tGCACCCCCGGGCTC‑TGGCGAAAAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGAGGGCTGCCCCGGCCtt  >  2:9329450/1‑140 (MQ=17)
                                                                                                                                         |                                                                                                                                            
TTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTC‑TGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTT  >  NZ_CP025268/2233103‑2233378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑