Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,233,2480GC25.1% 344.0 / 72.9 176intergenic (+148/+225)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC
Reads supporting (aligned to +/- strand):  ref base G (62/69);  new base C (44/0);  total (107/69)
Fisher's exact test for biased strand distribution p-value = 5.19e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 176 total aligned reads displayed.

TCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAAC  >  NZ_CP025268/2233113‑2233386
                                                                                                                                         |                                                                                                                                          
tcatcaGGTCCGAAAATAACGAGACTATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGcg                                                                                                                                        >  2:9524220/1‑139 (MQ=255)
  atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGC‑GGCCAAACGCCGCCCCCCCCAGCACCCCCCGGcgct                                                                                                                                      >  1:8554154/1‑140 (MQ=37)
  atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCCCCGCCCCCAGCACCCCCCGGcact                                                                                                                                      >  2:6752529/1‑140 (MQ=255)
       gTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCTGCCCCCTGCACCCCCGGGCTCTGGCGa                                                                                                                                 >  1:7937812/1‑140 (MQ=255)
            aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGG‑GGCCCAACGCCGCCCCCCCCACCACCCCCCGGCGCAGGCGGACaaa                                                                                                                            >  1:3918913/1‑140 (MQ=25)
                 aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGG‑GCCCAAATGCC‑CCCCCCCCAGCACCCCCCCGCGCGGGCGGACaaaaaaaa                                                                                                                       >  1:1201665/1‑136 (MQ=25)
                 aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGG‑GGCCAAACGCCGCCCCCCCCAGCACCCCCCCGCACAGGCGGACCAAATagc                                                                                                                       >  1:8054214/1‑140 (MQ=25)
                 aaCGAGAATATTTCAGTCTCTCAGCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGCCCAAAAAcgc                                                                                                                       >  2:1867434/1‑140 (MQ=25)
                  aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGGCCAAAC‑CCCCCGCCCCCAGCACCCCCCGGCTCTGGCGAACAAAATcgcc                                                                                                                      >  2:4466982/1‑140 (MQ=21)
                          atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCACGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGcgcg                                                                                                              <  1:262449/140‑1 (MQ=255)
                            ttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGC‑GGCCAAACGCCGCCGCCCCCAGCACCCCCCGGCTCTGGCGAACAAAAAAGCcgcggcgcggg                                                                                                            >  2:1475286/1‑139 (MQ=18)
                            ttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAAAGCCCCCCCCCCCATCACCCCCCGGCAAAGGCCAAAAAAAAAGCCGCTGCGCggg                                                                                                            >  2:5678576/1‑139 (MQ=18)
                             tCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTg                                                                                                           <  2:8560001/140‑1 (MQ=255)
                              cAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAACCCCGCCCCCCCCAGCACCCCCCGGCACAGGCGAACAAAATCGCCGCGGCGCGGTGc                                                                                                          >  2:5057598/1‑140 (MQ=18)
                                gTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCACTGGCGAACAAAATCGCCGCTGCGCGGTGccc                                                                                                        <  1:4920644/140‑1 (MQ=255)
                                 tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAACGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCAGCGCGGTGcccg                                                                                                       >  2:8895241/1‑139 (MQ=255)
                                   tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCCAAACCCCCCCCCCCCAGCACCCCCCCGCACAGGCGAACAAAAACACCCCGGCGCGGGGCCCACg                                                                                                     >  2:3370259/1‑140 (MQ=18)
                                     tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc                                                                                                   >  2:7014670/1‑140 (MQ=255)
                                      cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCt                                                                                                  <  2:3308495/140‑1 (MQ=255)
                                      cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCt                                                                                                  <  2:3307058/140‑1 (MQ=255)
                                        tCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTa                                                                                                <  1:7861887/140‑1 (MQ=255)
                                           tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAAAGCCCCCGCCCCCAGCACCCCCCGGCACTGGCGAACAAAATCGCCGCTGCGCCGTGCCCACGGCTTATcc                                                                                             >  2:8963005/1‑140 (MQ=18)
                                            gTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCACGGGGC‑GGCCCATCACCGCCCCCCCCAGCACCCCCCGGCTCTGGCGCACAAAAACGCCCCAGCGCGGTGCCCACGGCAAATccc                                                                                            >  2:2937375/1‑140 (MQ=18)
                                             ttGCGCTCATGTCATGTGCATTGCTTCATATAATCACTGTCTCAAGGAGCGC‑GCAGGGGGC‑TGCCAAACGACGCCCCACCCATCAACCCCGGGGACAGACGACACAAATCCCCGCTGAGCGGTGCCCTCGGCGTATCCCt                                                                                           >  2:6525618/1‑140 (MQ=16)
                                               gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGC‑GGCCAAAAGCCGCCGCCCCCAGCACCCCCCGGCAATTGCGAACAAAATAGCCCCTGAGCGGTGGCCTCAGCTAAtaacata                                                                                         >  2:2678000/1‑134 (MQ=17)
                                               gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCCCTGCGCGGTGCCCTCGGCTTATCCCTTa                                                                                         >  2:8006176/1‑140 (MQ=255)
                                               gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAAAGCCGCCCCCCCCAGCACCCCCCGGCACAGGCGAACAAAATCGCCGCAGCGCGGTGCCCAAGGCATATACCATa                                                                                         >  2:6518177/1‑140 (MQ=17)
                                                  cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCCATAGCCGCCCCCCCCAACACCCCCGGGGTCTGGCGAACAAAATCGCCGCAGAGCGGGGGCCGCGGCGTATACCTGACgg                                                                                      >  2:2920044/1‑140 (MQ=18)
                                                      tGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAc                                                                                  <  2:5491832/140‑1 (MQ=255)
                                                       gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCAGCCCCCCCGGGCTCAGGCGAACAAAATCGCCGCTGCGCGGTGCCCACGGCTGATCCCATACGGCTAcc                                                                                 >  2:7972748/1‑140 (MQ=17)
                                                       gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTGTGGCGAACAAAATCCCCCCTGCGCGGTGCCCACGGCTTATCCCTTAAGGCTAcc                                                                                 >  2:428103/1‑140 (MQ=17)
                                                       gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATAGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGACCACAATAGCCCCTGCGCGGTGCCCACGGCGGATCCCGGTCGGCTAcc                                                                                 >  2:4084308/1‑140 (MQ=17)
                                                             tgCCTTGCTTCATATACTCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCCCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCAGCGCGGTGCCCTCGGCTTATCCCGTACGGCTCCCGGGTCg                                                                           >  2:7842502/1‑140 (MQ=14)
                                                             tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGC‑GGCCCATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGAACCAAATCGCCGCGGCGCGGTGCCCGCGGCGTAAACCCTACGGCTCCCGGGTCg                                                                           >  2:6579217/1‑140 (MQ=14)
                                                             tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCACACGCCGCCCCCCCCGGCCCCCCCGGGGCCAGGCGCACCAAAACGCCGCGTCGCGGGGGCCGCGGCTTATCCCCTAAGGCGACCcgggag                                                                           >  2:3633476/1‑137 (MQ=9)
                                                             tgCATTGCTTCATATAATCACGGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCCAACGCCCCCCCCCCCGGCACCCCCCGGCTATGGCGCACAAAAACCCCGCTGCGCGGTGCCCGCGGCGTATCCCTTACGGCAACCCGGTCg                                                                           >  2:3986007/1‑140 (MQ=14)
                                                               cATTGCTTCATATAATCACTGGCGCACGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCGTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                                         <  1:7237650/140‑1 (MQ=255)
                                                               cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                                         <  1:2190557/140‑1 (MQ=255)
                                                                  tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGG‑GGGCCAAATCCCCCCCCCCCAGCACCCCCCGGCTAATGCGCACAAAATAGCCCCCGCGCGGTGGCCCACGCGGATACCAGACGGCAAACGGGTCGGgggc                                                                      >  2:2514418/1‑140 (MQ=255)
                                                                  tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCCCCCCCCCCTTCACCCCCGGGCTCAGGCGAACAAAATCGCCGCTGCGCGGGGCCCACGGCTTATACCTTAAGGCTACCGGGTCGGgcgc                                                                      >  2:1989732/1‑140 (MQ=14)
                                                                    cTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGGCAATCGCCGCCGCCCCCTGCACCCCCGGGGTCTGGCGCACAAAATCGCCGCTGCGCGGTGCCCTCCGCTTATCCCTTACGGCAACCGGGTCGGGCGCGa                                                                    >  2:9745036/1‑140 (MQ=14)
                                                                         tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                               <  2:7204476/140‑1 (MQ=37)
                                                                         tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                               <  1:300170/140‑1 (MQ=37)
                                                                         tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                               <  2:7405424/140‑1 (MQ=37)
                                                                         tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATAGCCGCCGCCCCCTGCACCCCCGGGCACTGGCGAACAAAATAACCGCTGCGCGGTGCCCTAGGCATATACCATACTGCTACCAGGTCGGGCGCGAGGGaa                                                               >  2:4986458/1‑140 (MQ=11)
                                                                          ataATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑CGCCAAAACCCCCCACCCCCAGCACCCCCAGGCACAGGCGAACAAAAAAGCCCCAGCCCCGTGCCCAAGGCTAAAACCCTACCGCTACCCGGGCGGGCGCGAGGTAAc                                                              >  2:9413089/1‑140 (MQ=2)
                                                                           taATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                                             <  2:4024790/140‑1 (MQ=35)
                                                                           taATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                                             <  2:9561341/140‑1 (MQ=35)
                                                                            tgccACTGGCGCAAGGAGCGC‑GCATGGGGC‑GGCCCAACGCCGCCGCCCCCTGCACCCCCGGGCTCAGGCGAACAAAATCGCCGCTGCGCGGGGCCCACGGCGTATCCCTTACCGCGACCGGGGAGGGCGCCAGGTaaaaa                                                            >  2:2923987/4‑137 (MQ=1)
                                                                            aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                                            <  1:1503369/140‑1 (MQ=34)
                                                                            aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                                            <  1:1503198/140‑1 (MQ=34)
                                                                            aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAACGCCGCCGCCCCCAGCACCCCCCGGCACTGGCGAAACAAATCGCCCCAGCCCGGTGCCCACGGCGTAACCCAAAAGGCTACCCGGTCTCGCGCGAGGTAACaa                                                            >  2:8925244/1‑139 (MQ=2)
                                                                             aTCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATc                                                           <  1:3495870/140‑1 (MQ=33)
                                                                              tCACTGGCGCAAGGAGCGC‑GCAGGGGGG‑GGCCAAACGCCGCCCCCCCCATCCCCCCCCCGCTCAGGCGAACAAAAACGCCGCGGCGCGGGGGCCACGGCGTATCCCATACGGGGACCCCGTCGGGCGCGAGGGaaaaaac                                                          >  2:8282819/1‑135 (MQ=2)
                                                                              tCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCCAAACCCCCCCCCCCCCGCCCCCCCCGGCGCGGGCGCACAAAAAAGCCCCTGCGCGGGGCCCCCCGCGTATACCATAAGGCGACCCGGGCGGGCGCGCGGGaaaaacc                                                          >  2:1503334/1‑135 (MQ=2)
                                                                              tCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAAACCCCGCCCCCCCCAGCACCCCCCGGCTCAGGCGAACCAAAAACCCCCAGCGCCGTGCCCACCGCCTATACCATACGGCTACCCCGGAGGGCGCCAGGTAACaaac                                                          >  2:9187930/1‑137 (MQ=2)
                                                                                   ggCGCAAGGAGCGC‑GCAGGGGGG‑GGCCCAAAGCCCCCGCCCCCAGCACCCCCCGGCGCATGCGAAAAAAAAAGCCCCAGCGCGGTGCCCACGGCTTATCACATAAAGCATCCCGGTCGGGGGCGAGGGTACATACCAGTa                                                     >  2:6397653/1‑140 (MQ=2)
                                                                                        aaGGAGCGC‑GCAGGGGGC‑GGCCACTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGGGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCAGTAACAcg                                                <  2:1653381/140‑1 (MQ=255)
                                                                                        aaGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAACTCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                                                <  2:1653344/140‑1 (MQ=17)
                                                                                        aaGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                                                <  1:6752529/140‑1 (MQ=32)
                                                                                        aaGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                                                <  2:7611827/140‑1 (MQ=32)
                                                                                        aaGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                                                <  1:3799966/140‑1 (MQ=32)
                                                                                         aGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgc                                               <  1:4347347/140‑1 (MQ=32)
                                                                                            aGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGcc                                            <  2:6112969/140‑1 (MQ=32)
                                                                                              cgc‑gcAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGTGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                          <  2:2254859/140‑1 (MQ=17)
                                                                                              cgc‑gcAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                          <  2:3417526/140‑1 (MQ=32)
                                                                                              cgc‑gcAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                          <  2:2280211/140‑1 (MQ=32)
                                                                                                      ggggC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCca                                   <  1:6082809/140‑1 (MQ=32)
                                                                                                        ggc‑ggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                                 <  2:3770658/140‑1 (MQ=32)
                                                                                                        ggc‑ggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                                 <  1:2635215/140‑1 (MQ=32)
                                                                                                        ggc‑ggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                                 <  2:576749/140‑1 (MQ=32)
                                                                                                         gc‑ggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACAt                                <  2:3910561/140‑1 (MQ=32)
                                                                                                            ggcCAAACGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGAAAAAAAACGCCGCTGCGCGGTGCCCACGGCTTATACCTTACGGCTACCCGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCACAGCCCACAAcc                              >  2:7160591/1‑140 (MQ=11)
                                                                                                             gcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa                             >  2:6001545/1‑140 (MQ=32)
                                                                                                             gcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa                             <  1:2235252/140‑1 (MQ=32)
                                                                                                             gcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa                             >  1:1801736/1‑140 (MQ=32)
                                                                                                             gcCAATCGCCGCCGCCCCCAGCACCCCCGGGCACTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCGTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa                             >  1:5985244/1‑140 (MQ=12)
                                                                                                              ccAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAG‑CCACATCCAtg                           <  1:6694861/140‑1 (MQ=17)
                                                                                                              ccAATCGCCGCCGCCCCCAGCACCCCCCGGCTCTGGCGAACAAAATAGACGCTGCGCGGTGCCCTCGGCTTATCCCTGACGGCTACCGGGTCGGGCGCGAGGTCACCTCCCTGTAAATAGCGCCCTCAGCCCACATCCAt                            >  1:9146468/1‑140 (MQ=11)
                                                                                                               cAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtg                           <  2:108093/140‑1 (MQ=32)
                                                                                                                 aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTCACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                         >  1:1368734/1‑140 (MQ=17)
                                                                                                                 aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                         >  1:200183/1‑140 (MQ=32)
                                                                                                                   cgccgccgcCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg                       >  1:5851307/1‑140 (MQ=32)
                                                                                                                   cgccgccgcCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg                       >  1:9431202/1‑140 (MQ=32)
                                                                                                                     ccgccgccCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCt                     >  1:9182101/1‑140 (MQ=32)
                                                                                                                      cgccgccCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTg                    >  1:4949663/1‑140 (MQ=32)
                                                                                                                        ccgccCCCTGCACCCACGGGCTCTGGCGAACAAAATCGCCGCTGCTCGGTGCCCTCGGCGTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGATGcc                  >  1:2165079/1‑140 (MQ=255)
                                                                                                                          gccCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcccc                <  2:6860066/140‑1 (MQ=32)
                                                                                                                          gccCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcccc                <  2:8054214/140‑1 (MQ=32)
                                                                                                                            ccccTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCgg              >  2:326837/1‑140 (MQ=32)
                                                                                                                             cccTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCATTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGGGGGCTGCCCCGGc             <  2:1514210/140‑1 (MQ=17)
                                                                                                                                tGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCtt          <  1:11219/140‑1 (MQ=32)
                                                                                                                                tGCACCCCCGGGCTCTGGCGAAAAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGAGGGCTGCCCCGGCCtt          >  2:9329450/1‑140 (MQ=17)
                                                                                                                                 gCACCCCCGGGCACTGGCGTAAAAAAGCGCCGCTGCGCGGTGCCCTCGGCTAATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACGTCCCGGTTGAACGCGCCCTCAGCCCGCGTGCATGTGGGCTGCCCCGGCCTTc         >  2:111920/1‑140 (MQ=11)
                                                                                                                                  ctcccccGGGCTCTGGCGAACAATTTCGCCGCTGCGCGGGGCGCTCGGCTGTTCCCTTACGGCTCCCGGGGCGGGCGCGCGGTAACGTCCCGGGGCTACGCGCCCTCAGCCGTCCTCCGGGGGGGCGGCCCCGGccggcg        >  2:5803684/3‑136 (MQ=2)
                                                                                                                                     ccccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAggg     >  1:2822734/1‑140 (MQ=32)
                                                                                                                                      cccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGa    >  1:1588077/1‑140 (MQ=32)
                                                                                                                                       ccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGCCTACCGGGTCGGGCGCGAGGTACCATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGac   >  2:2237266/1‑139 (MQ=12)
                                                                                                                                       ccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCGTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGaa   >  1:2338285/1‑140 (MQ=17)
                                                                                                                                        cGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAAc  >  2:145828/1‑140 (MQ=32)
                                                                                                                                         |                                                                                                                                          
TCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAAC  >  NZ_CP025268/2233113‑2233386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑