Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,505,5530TA24.0% 546.4 / 62.2 266intergenic (+84/+68)phoB/yhfSDNA‑binding transcriptional regulator FrlR/aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base T (34/150);  new base A (58/0);  total (112/154)
Fisher's exact test for biased strand distribution p-value = 3.96e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 267 total aligned reads displayed.

AACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATG  >  NZ_CP025268/3505414‑3505690
                                                                                                                                           |                                                                                                                                          
 aCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTa                                                                                                                                           <  1:5038710/140‑1 (MQ=255)
   tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAc                                                                                                                                         <  2:7356680/140‑1 (MQ=255)
    cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                        <  1:990027/140‑1 (MQ=255)
    cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                        <  1:2237547/140‑1 (MQ=255)
    cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                        <  2:4070637/140‑1 (MQ=255)
    cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                        <  2:3972600/140‑1 (MQ=255)
      tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                      <  1:6099290/140‑1 (MQ=255)
      tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                      <  2:3424054/140‑1 (MQ=255)
      tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                      <  1:5413675/140‑1 (MQ=255)
      tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                      <  1:4114089/140‑1 (MQ=255)
      tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                      <  2:8121287/140‑1 (MQ=255)
      tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                      <  1:849136/140‑1 (MQ=255)
      tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                      <  1:7692088/140‑1 (MQ=255)
      tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAAacca                                                                                                                                      >  1:4965032/1‑139 (MQ=255)
             cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                                               <  1:5854857/140‑1 (MQ=255)
               ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAACCCGCTCCCCcaa                                                                                                                             >  1:8722010/1‑140 (MQ=255)
               ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCAAAACCACTCCCCcaa                                                                                                                             >  1:742827/1‑140 (MQ=39)
                 aagcGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAACgg                                                                                                                           <  1:6556074/137‑1 (MQ=255)
                 aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                           <  1:8233748/140‑1 (MQ=255)
                   tCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgggg                                                                                                                         <  1:160782/140‑1 (MQ=255)
                      cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                      <  2:3631256/140‑1 (MQ=255)
                        tCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCCAAACCACACCCCAAACGGGCgg‑gg                                                                                                                   >  1:3266134/1‑140 (MQ=38)
                           ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGa                                                                                                                <  1:247976/140‑1 (MQ=255)
                                ccTATTGATAATAAAAGACATTCACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACCGGGGCGA‑GGGGACCGtt                                                                                                           <  2:267001/139‑1 (MQ=255)
                                ccTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATCTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGCGGCGA‑GGGGACCGtt                                                                                                           <  2:2406347/139‑1 (MQ=255)
                                aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGtt                                                                                                           <  1:5293273/140‑1 (MQ=255)
                                aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTGTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGtt                                                                                                           <  2:6673636/140‑1 (MQ=255)
                                aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAACTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGtt                                                                                                           <  1:4546944/140‑1 (MQ=255)
                                 cTATTGATAATAAAAGACATTAACCGTAGGCCTGATAATATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCAAAACCAAAACCAAAACCCAAAAGGGCGATGGGGACGGta                                                                                                           >  2:17111/1‑139 (MQ=25)
                                  tATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCa                                                                                                         <  2:8754653/140‑1 (MQ=255)
                                    ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGt                                                                                                       >  2:7026358/1‑140 (MQ=255)
                                    ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACAACCAAAACCAAAACCCAAAAGGGGGA‑GGGGGCCGTTAAGt                                                                                                       >  1:4755700/1‑140 (MQ=25)
                                     tGATAATAAAAGACATTAACCTTAGGCCGGATAAGATGCGACAGCATCGCATCCGGCGATGCTGGGGCGTTGAAATTTACATCACGTACGGTCCCCACTCCCTAACCCTCTCCCCAAACGGGCGT‑GGGGACCGGTCACTa                                                                                                      >  2:7148403/1‑140 (MQ=255)
                                     tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                      <  2:4918416/109‑1 (MQ=255)
                                     tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTa                                                                                                      >  2:2809913/1‑140 (MQ=255)
                                     tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTa                                                                                                      <  1:2814200/140‑1 (MQ=255)
                                     tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTa                                                                                                      >  2:9728474/1‑140 (MQ=255)
                                     tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCAAACCCTCTCCCCCAAGGGGCGA‑GGGGACCGTTCAGTa                                                                                                      >  2:9142446/1‑140 (MQ=255)
                                     tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCAAAACCACTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTa                                                                                                      >  2:4799094/1‑140 (MQ=255)
                                     tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCGAACCCCCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTa                                                                                                      >  1:6740766/1‑140 (MQ=255)
                                     tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCCCCCCCAAAACCACGACCCCAACCGGCGA‑GGGGGCCGGTCAGtc                                                                                                      >  1:7282247/1‑139 (MQ=25)
                                     tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCGCACCCTAACCCACACCCCCAATGGGCGA‑GGGGTCCGGTCAGTa                                                                                                      >  1:5727159/1‑140 (MQ=38)
                                      gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTAc                                                                                                     >  2:5856709/1‑140 (MQ=255)
                                      gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAAACCACAACCCCAATGGGCGA‑GGGGTCCGTTAAGTaa                                                                                                     >  1:9527825/1‑139 (MQ=37)
                                      gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACAACCCAAAGGGGCGA‑GGGGACCCGTCAGTaa                                                                                                     >  2:2996388/1‑139 (MQ=38)
                                      gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCAAAACCCAAATGGGCGA‑GGGGGCCGTTAAGTAc                                                                                                     >  1:7681698/1‑140 (MQ=37)
                                      gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCAACCCCCAAACCACGACCCAAATGGGCGC‑GGGGGCCGGTCAGTAc                                                                                                     >  1:3650945/1‑140 (MQ=25)
                                              aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCAAACCCAAACCAGCTACCCAAATGGGCGA‑TGGGACCGTTCATTTAGGAATTTa                                                                                             >  2:3643544/1‑140 (MQ=37)
                                                 aCATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCt                                                                                          <  2:4587288/140‑1 (MQ=255)
                                                   aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAACCTAACCCACAACCCAAAGGGGCGA‑TGGGACCGTTCAGTTAGGAATTTTGCttt                                                                                        >  1:2988364/1‑139 (MQ=37)
                                                    ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                       <  1:1091035/140‑1 (MQ=255)
                                                    ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                       <  1:1861890/140‑1 (MQ=255)
                                                    ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                       <  1:7277629/140‑1 (MQ=255)
                                                    ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                       <  1:4815873/140‑1 (MQ=255)
                                                    gtAACCGCAGGCCGGATAAGATGCGCCAGCATCGCCTCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGCGA‑GGGGACCTTTCAGTACGGAATTTAGCTTGa                                                                                       <  2:8555816/139‑1 (MQ=255)
                                                      aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCAAAACCCAAAAGGGCGA‑GGGGACCGTTCAGTAACGAATTTATCTTTACg                                                                                     >  1:896459/1‑140 (MQ=37)
                                                         cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  1:36863/140‑1 (MQ=255)
                                                         cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  1:3310773/140‑1 (MQ=255)
                                                         cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  1:9328978/140‑1 (MQ=255)
                                                         cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  1:3431352/140‑1 (MQ=255)
                                                         cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  2:1227984/140‑1 (MQ=255)
                                                           tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAACCGAACCCTAAACCCAAAGGGGCGT‑GGGGACCGTTCACTAAGGAAATTATCTTGACGGTGCt                                                                                >  1:7852979/1‑140 (MQ=37)
                                                            aGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                             >  1:8905861/1‑95 (MQ=255)
                                                            aGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCTCTCCCCAAATGGGCGA‑GGGGAACGTTCATTAAGGAAATTATCTTTACGGTGCTa                                                                               >  2:1324313/1‑140 (MQ=38)
                                                             ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCCAAAACAAAACCCAAAAGGGCGA‑CGGGAACCTGCAGTAACCCATTTATCTTGACGGGGcgaa                                                                              >  1:6345622/1‑137 (MQ=16)
                                                             ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCTAACCCACTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCATGACGGTGCTAt                                                                              >  2:5024696/1‑140 (MQ=255)
                                                              gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                             <  2:5902754/140‑1 (MQ=255)
                                                              gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                             <  1:2619069/140‑1 (MQ=255)
                                                                cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                           <  1:1391929/140‑1 (MQ=255)
                                                                  gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACCACCAAACCCACTACCCCAAAGGGCGA‑GGGGCACGGTAACTACGGAAATAAGCTTGACCGTGGTATCCacc                                                                         >  1:9695333/1‑138 (MQ=255)
                                                                  gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCGCTCCCCACAGGGGCGA‑GGGGACCGTTCACTACGGAATTTAGCTTTAAGGTGCTCTCCAGc                                                                         >  1:6525704/1‑140 (MQ=255)
                                                                  gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACTCCCCAAATGGGCGA‑GGGGACCCGTCAGTACGGAATTTATCGTGAAGGTGCGATCCCGc                                                                         >  2:1486126/1‑140 (MQ=255)
                                                                  gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCACACCCCAAAAGGGCGT‑GGGGACCGGTCAGTAAGGAATTTATCTTTTAGGTGCTAtaaagc                                                                         >  1:5721558/1‑135 (MQ=255)
                                                                   aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCCAAACCACACCCCCAAAGGGCGG‑GGGGGCCGGTCAGTAAGGAAATTAGATTGACGGTGCAATACAGCa                                                                        >  1:3922188/1‑140 (MQ=255)
                                                                   aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCACACCCAAAACCACTACCCCAACGGGCGA‑GGGGACCGGTCAGTACGGAATTTAGCATGTCGGGGCTATCCCGcc                                                                        >  1:1700112/1‑139 (MQ=255)
                                                                     aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                      <  2:1117382/140‑1 (MQ=255)
                                                                          gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATa                                                                 <  2:2016117/140‑1 (MQ=255)
                                                                          gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATa                                                                 <  1:7994865/140‑1 (MQ=255)
                                                                          gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCTGCATTAAATa                                                                 <  1:205943/140‑1 (MQ=255)
                                                                           cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                <  2:9207583/140‑1 (MQ=255)
                                                                            gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCCAACCCACAACCCCAAGGGGCGC‑GGGGGCCCTTCAGTACCGCATTTAGCTTTACGGTGCTATACAGCATTAAATaat                                                               >  1:7055311/1‑138 (MQ=255)
                                                                            gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAACCCACAACCCCAACGGGCGA‑GGGGGCCGGTCAGTACGGAAATTTGCTTGAAGGTGGGATACAGCAGTTAATaat                                                               >  1:9484576/1‑138 (MQ=255)
                                                                             ccAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                              <  1:6944944/140‑1 (MQ=255)
                                                                             ccAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACTCCCCAAAAGGGCGA‑GGGGACCGGTCAGTACGGAAATTAGCTTGAAGGTGCTATCCAGCCTTAAATaaag                                                              >  1:6617765/1‑137 (MQ=255)
                                                                                 catcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCaa                                                          <  1:126473/140‑1 (MQ=255)
                                                                                          cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                       >  2:6527370/1‑102 (MQ=255)
                                                                                            gCGATGCTGGCGCGGTGAATTTTACATCCCGTACGTGCCCCACACCCGAACCCGCACCCCACAGGGGCGC‑GGGGTCCGTTCAGTACGGAATTTAGCTTGGCGGTGCAAGACCGCATTAAATAATGGCCAAACACACACGc                                               >  2:3555279/1‑140 (MQ=255)
                                                                                             cGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCa                                              <  1:5827731/140‑1 (MQ=255)
                                                                                             cGATGCTGGCGCGTTGAATTTGACATCCCGTACGGTCCCCTCCCCCGAACCCACTCCCCACAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCa                                              <  1:802436/140‑1 (MQ=255)
                                                                                               aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                            <  2:179448/140‑1 (MQ=255)
                                                                                                    ggCGCGTAGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                       <  2:7623341/140‑1 (MQ=255)
                                                                                                      cgcgTAGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGA‑GGGGACCGTACAGTACGGAAATTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgca                                     <  1:9761240/140‑1 (MQ=38)
                                                                                                          ttGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACTCCCCAAAGGGGCGG‑GGGGTCCCTTCAGTAACGAATTTATCTTGACCGGGCTAACCCCCCTTAAATAAAGTCAAAAAACCCACGCCAAAACCCCAGCAc                                 >  2:4249436/1‑140 (MQ=255)
                                                                                                          ttGAATTTGACATCCCGTACGGTCCCCTCACCCTACCCCTCGCCCCCACGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTGGCCGGGGCGATCCAGCATTAAAGACGGGCAATACTCTCCCGCAAAATCCGCAGCAc                                 <  2:9567633/140‑1 (MQ=255)
                                                                                                                  tACATCCCGTACGGTCCCCTAACCCGAACCCTCTCCCCAAATGGGCGA‑GGGGTGCGGTCACTAAGGAAATTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATAATCTCACGCAAAATCCGCAGCACCGGCGCtt                         >  2:6296872/1‑140 (MQ=255)
                                                                                                                           tACGTTCCCCTCACCCAAAACCACTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCAATCCAGCATTAAATACTGGCAATACTCTCACGCAAAAACCGCAGCACCGTCACTTCACCGCTGc                >  2:7773303/1‑140 (MQ=39)
                                                                                                                           tACGTTCCCCACACCCAAAACCAAACCCCCAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTAAACACCAATAAAAACATGCAATACTCTCACCCAAAATACGCAACACCCTCTCTTCACCGCTGc                >  2:889386/1‑140 (MQ=255)
                                                                                                                               ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGTTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAtt            <  1:4091485/140‑1 (MQ=255)
                                                                                                                                   ccTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCggg        <  1:9059398/140‑1 (MQ=255)
                                                                                                                                        cccAAACCCGCACCCCACAGGGGCGA‑GGGGCCCGGTCAGTACGGAATTTAGCTTGACCGTGCTATCCCGCCATAAATACAGGCAAAACACTCACGCAAAAACCCCCGCACCCGCGCGTCACCCCTGCCAGTCGGGGTAAt   >  2:5347516/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATG  >  NZ_CP025268/3505414‑3505690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑