Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,505,5610TA22.4% 507.9 / 52.5 246intergenic (+92/+60)phoB/yhfSDNA‑binding transcriptional regulator FrlR/aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base T (59/128);  new base A (54/0);  total (117/129)
Fisher's exact test for biased strand distribution p-value = 2.35e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 305 total aligned reads displayed.

TGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCAC  >  NZ_CP025268/3505422‑3505700
                                                                                                                                           |                                                                                                                                            
     cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTAAACCACTCCCCa                                                                                                                                         >  2:4938493/1‑140 (MQ=255)
     cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                                                         <  1:5854807/140‑1 (MQ=255)
       ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                       <  1:5676313/140‑1 (MQ=255)
              cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                                <  2:3631256/140‑1 (MQ=255)
              cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                                <  1:7997442/140‑1 (MQ=255)
                tCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCCAAACCACACCCCAAACGGGCgg‑gg                                                                                                                             >  1:3266134/1‑140 (MQ=38)
                   ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGa                                                                                                                          <  2:7967692/140‑1 (MQ=255)
                   ccTGAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTAGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTCCCCCACGGGGCGA‑GGGGa                                                                                                                          <  1:2263286/140‑1 (MQ=39)
                    cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCAAAGGGGCGA‑GGGGAc                                                                                                                         >  2:3878623/1‑140 (MQ=255)
                        aCTATTGATAATCAAAGACATTAACCGTAGTCCGGAGTATAGGCGCCCGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTCCCTCCCGTACGTTCCCCTCCCCCGAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGtt                                                                                                                     <  2:8654276/140‑1 (MQ=37)
                        aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTGTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGtt                                                                                                                     <  2:6673636/140‑1 (MQ=255)
                        aCTAGTGATAATAATAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCTTTGAATTTTACATCCCGTACGTTCCCCTCACCGTATACCTCTCCCCAAAGGGGCGA‑GGGGACCGtt                                                                                                                     <  2:9718689/140‑1 (MQ=38)
                         cTATTGATAATAAAAGACATTAACCGTAGGCCTGATAATATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCAAAACCAAAACCAAAACCCAAAAGGGCGATGGGGACGGta                                                                                                                     >  2:17111/1‑139 (MQ=25)
                            ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACACTCTCCCCCAAGGGGCGA‑GGGGCCCGTTCAGt                                                                                                                 >  1:7356253/1‑140 (MQ=255)
                             tGATAATAAAAGACATTAACCTTAGGCCGGATAAGATGCGACAGCATCGCATCCGGCGATGCTGGGGCGTTGAAATTTACATCACGTACGGTCCCCACTCCCTAACCCTCTCCCCAAACGGGCGT‑GGGGACCGGTCACTa                                                                                                                >  2:7148403/1‑140 (MQ=255)
                             tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGGTCAGTa                                                                                                                >  1:6740886/1‑140 (MQ=255)
                             tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCCAAACCACACCCCAAAAGGGCGA‑GGGGACCCGTCAGTa                                                                                                                >  1:3909922/1‑140 (MQ=38)
                             tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCAAACCCTCTCCCCCAAGGGGCGA‑GGGGACCGTTCAGTa                                                                                                                >  2:9142446/1‑140 (MQ=255)
                             tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCAAAACCACTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTa                                                                                                                >  2:4799094/1‑140 (MQ=255)
                             tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCGCACCCTAACCCACACCCCCAATGGGCGA‑GGGGTCCGGTCAGTa                                                                                                                >  1:5727159/1‑140 (MQ=38)
                                 aataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTTCCCACACCCAAAACCACACCCCCAAGGGGCGA‑GGGGGCCCTTCAGtgacgg                                                                                                            >  1:471646/1‑135 (MQ=37)
                                    aaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAAtt                                                                                                         <  1:65974/140‑1 (MQ=255)
                                    aaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCCACCAAAACCACAACCCAAAAGGGCGA‑GGGGTCCCGTCAGGTCGGGAtt                                                                                                         >  1:4637770/1‑140 (MQ=25)
                                      aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGGTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCTCTCCCCAAAGGGGCGA‑GGGGACAGTTCAGTACGGAAGTTa                                                                                                       >  2:5181651/1‑140 (MQ=255)
                                          cATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCAAAGGGGCGC‑GGGGACCGGTCAGTAAGGAATTTAGCtt                                                                                                   >  1:1843812/1‑140 (MQ=38)
                                           aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAACCTAACCCACAACCCAAAGGGGCGA‑TGGGACCGTTCAGTTAGGAATTTTGCttt                                                                                                  >  1:2988364/1‑139 (MQ=37)
                                            ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACCTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 <  2:2746034/140‑1 (MQ=255)
                                            ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 <  1:7026358/140‑1 (MQ=255)
                                            ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 <  1:7277629/140‑1 (MQ=255)
                                            ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 <  1:4815873/140‑1 (MQ=255)
                                              aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCAAAACCCAAAAGGGCGA‑GGGGACCGTTCAGTAACGAATTTATCTTTACg                                                                                               >  1:896459/1‑140 (MQ=37)
                                                 cggaGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                            <  1:3666700/137‑1 (MQ=255)
                                                 cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                            <  1:5331741/140‑1 (MQ=255)
                                                 cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                            <  1:3431352/140‑1 (MQ=255)
                                                 cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                            <  1:9328978/140‑1 (MQ=255)
                                                 cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                            <  2:1227984/140‑1 (MQ=255)
                                                 cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                            <  1:3311637/140‑1 (MQ=255)
                                                  gTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTAACCCGCTACCCAAAGGGGCGA‑GGGGCCCGTTCAGTACGGAATTTAGCTTGACGGTGc                                                                                           >  2:2906174/1‑140 (MQ=255)
                                                   tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCACCCCCAACGGGGGA‑GGGGACCGTTCAGTACGGAAATTAGCTTGCCGGTGcg                                                                                          >  1:6701195/1‑139 (MQ=38)
                                                    aGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                       <  2:8905861/95‑1 (MQ=255)
                                                     ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCCAAAACAAAACCCAAAAGGGCGA‑CGGGAACCTGCAGTAACCCATTTATCTTGACGGGGcgaa                                                                                        >  1:6345622/1‑137 (MQ=16)
                                                     ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCAAAACCGCTCCCCACAGGGGGGT‑GGGGCCCGGTGCGTCCGGGATTTACCTGGGCGGGGCtgt                                                                                        >  1:1549933/1‑138 (MQ=21)
                                                     ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACTACCCAAAGGGGCGA‑GGGGACCGTTCAGTAAGGAAATTAGCTTTAAGGTGCTAt                                                                                        >  2:4353315/1‑140 (MQ=38)
                                                     ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACACAAAACCCCACCCCCAAGGGGCGC‑GGGGTCCGGGCAGTACGGAATTTAGGATTACGGTGcgaa                                                                                        >  1:990895/1‑137 (MQ=25)
                                                     ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCACACCCAAAACCACACCCCAAAGGGGCGA‑GGGGACCGGTAAGTACGGAATTTAGCTTTACGGTGCTaa                                                                                        >  1:8821547/1‑139 (MQ=38)
                                                      gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                       <  1:2011758/140‑1 (MQ=255)
                                                        cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                     <  1:8545595/140‑1 (MQ=255)
                                                        cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                     <  1:1391929/140‑1 (MQ=255)
                                                          gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCCCAACCCAAAGGGGCGG‑GGGGGCCGGTAAGTACGGAATTTATCTTGACGGTGGTAAACAGc                                                                                   >  1:716934/1‑140 (MQ=255)
                                                          gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCGCTCCCCACAGGGGCGA‑GGGGACCGTTCACTACGGAATTTAGCTTTAAGGTGCTCTCCAGc                                                                                   >  1:6525704/1‑140 (MQ=255)
                                                          gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACTCCCCAAATGGGCGA‑GGGGACCCGTCAGTACGGAATTTATCGTGAAGGTGCGATCCCGc                                                                                   >  2:1486126/1‑140 (MQ=255)
                                                           aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCAAACCCAAAACCACAACCCAAAGGGGCGA‑GGGGACCCTTCAGTAAGGAATTTAGCTTGTCGGTGGTATCCAGCa                                                                                  >  1:1700066/1‑140 (MQ=255)
                                                           aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCa                                                                                  <  2:2273358/140‑1 (MQ=255)
                                                           aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTAAGGAATTTAGCTTGAAGGTGCGATCCAGCa                                                                                  >  2:9534386/1‑140 (MQ=255)
                                                             aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                                <  2:1117382/140‑1 (MQ=255)
                                                                    gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAACCCACAACCCCAACGGGCGA‑GGGGGCCGGTCAGTACGGAAATTTGCTTGAAGGTGGGATACAGCAGTTAATaat                                                                         >  1:9484576/1‑138 (MQ=255)
                                                                      cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                                       <  1:2898214/140‑1 (MQ=255)
                                                                              catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCCCCCCCAAAACCACAACCCCAAGGGGCGG‑GGGGGCCCGTACGTAAGGAATTTAGCGTGTAGGGGGTATACAGCATTAAAAAATTGCAATaaaa                                                               >  1:9593155/1‑137 (MQ=255)
                                                                                  cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 >  2:6527370/1‑102 (MQ=255)
                                                                                    gCGATGCTGGCGCGGTGAATTTTACATCCCGTACGTGCCCCACACCCGAACCCGCACCCCACAGGGGCGC‑GGGGTCCGTTCAGTACGGAATTTAGCTTGGCGGTGCAAGACCGCATTAAATAATGGCCAAACACACACGc                                                         >  2:3555279/1‑140 (MQ=255)
                                                                                       aagCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTAACCCTCTCCCCACAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                      <  2:8876343/138‑1 (MQ=255)
                                                                                       aTGCTGGCGCTTTGAATTTTACATCCCGTACGTTCCCCTCACCCTCACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                      <  2:7429956/140‑1 (MQ=255)
                                                                                       aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                      <  2:6800196/140‑1 (MQ=255)
                                                                                        tGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaaa                                                     >  2:1872846/1‑140 (MQ=255)
                                                                                              cgcgTTGAATTTTACATCCCGTACGTTACCCTCACCCTAACCCGCACCCCCAAAGGGCGT‑GGGGAACGGTCAGTAACGAATTTATCTTGACGGTGCTATACCGCATTAAATACGGGCAATACTCTCACGCAAAATCCgca                                               >  2:4067352/1‑140 (MQ=255)
                                                                                                cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcagc                                             <  1:5823774/140‑1 (MQ=255)
                                                                                                  ttGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCTCAACCCCAACGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACCGTGCTATCCCGCCTTAAATACTGGCCATACTCTCACGCAAAATCCGCAGCAc                                           >  2:7297617/1‑140 (MQ=255)
                                                                                                        tttACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtctc                                     <  1:7911106/140‑1 (MQ=255)
                                                                                                                   tACGTTCCCCTAAACCTAACCCTCTCCCCAAATGGGCGA‑GGGGACCGTTCAGTACGGAAATTACCTTTACGGAGCTATCAAGCGTTAAATACTGGCACTACTCTCACGCACAATCCGCAGCACCGTCTCTTGACCGCttc                          >  2:7773302/1‑138 (MQ=255)
                                                                                                                    aCGTTCCCCTCCCCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCg                         <  1:9699104/140‑1 (MQ=255)
                                                                                                                       ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGTTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAtt                      <  1:4091485/140‑1 (MQ=255)
                                                                                                                          cccTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAt                                                                                 >  2:6139393/1‑78 (MQ=255)
                                                                                                                           ccTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCggg                  <  1:9059398/140‑1 (MQ=255)
                                                                                                                            cTCACCCTACCCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAGTCGGGt                 <  1:7812827/140‑1 (MQ=255)
                                                                                                                             tCACCCAAAACCTCACCCCAAAGGGGCGA‑GGGGACCGTTAAGTACGGAAATTAGCATTACGGTGCTATCCATCATTAAATACTGGCAAAACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGtt                >  2:5876050/1‑140 (MQ=255)
                                                                                                                                cccTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt             <  2:4594563/140‑1 (MQ=255)
                                                                                                                                 ccTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATg            >  1:256485/1‑140 (MQ=255)
                                                                                                                                    aaCCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGTCGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa         >  1:5185531/1‑140 (MQ=255)
                                                                                                                                    aaCCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                                        >  2:6949527/1‑109 (MQ=255)
                                                                                                                                     aCCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGac        >  1:4354005/1‑139 (MQ=255)
                                                                                                                                     aCCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa        >  2:20258/1‑140 (MQ=255)
                                                                                                                                      cccTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAAt       >  1:1116115/1‑140 (MQ=255)
                                                                                                                                         tctcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc    >  2:314187/1‑140 (MQ=255)
                                                                                                                                         tctcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc    >  1:2710477/1‑140 (MQ=255)
                                                                                                                                          ctcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCACAATCCGCAGCACCGGCTCTGCACCGCTGCGATTCGGGTTAATGCGAATCGca   >  2:5829189/1‑140 (MQ=255)
                                                                                                                                          ctcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGca   <  1:894244/140‑1 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGCATCGcac  >  2:5565324/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:1058366/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:1338022/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  2:9360680/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  <  1:3789135/140‑1 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:4995481/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:6078813/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:7536578/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:7860013/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:8131457/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  2:2664742/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGGTAATGCGAATCGcac  >  2:9063961/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGGCTCTTCACCGCTGCGAGGCGGGTTCATGCGAATCGcac  >  2:8534563/1‑140 (MQ=255)
                                                                                                                                           tcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAAACCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:8061200/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCAC  >  NZ_CP025268/3505422‑3505700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑