BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,292,813	C→T	noncoding (46/132 nt)	CXP41_RS06830 ←	RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,292,813	0	C	T	93.2%	705.3 / 29.2	350	noncoding (46/132 nt)	CXP41_RS06830	RtT sRNA
Reads supporting (aligned to +/- strand): ref base C (9/14); new base T (75/241); total (92/258)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 351 total aligned reads displayed.

ATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGG  >  NZ_CP025268/1292676‑1292951

aTCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGCAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCCAGTCg                                                                                                                                          <  2:4301577/140‑1 (MQ=255)

 tCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCACCGCTCTCTCGCTGGCGCTCAAGTCGa                                                                                                                                         >  1:8465906/1‑140 (MQ=255)

    ggCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAAcc                                                                                                                                      <  1:7570055/140‑1 (MQ=255)

    ggCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCACTCTCGCTGGCGCGCAAGTCGaagc                                                                                                                                      >  1:2770678/1‑138 (MQ=21)

         aaTTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCttggt                                                                                                                                 >  1:7344815/1‑137 (MQ=255)

            tAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGa                                                                                                                              <  2:9438852/140‑1 (MQ=255)

            tAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGa                                                                                                                              <  1:1274373/140‑1 (MQ=255)

            tAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGa                                                                                                                              <  2:3536436/140‑1 (MQ=255)

             aGCTCGGAGTAACAGGTTTTGATTATTTATGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGaa                                                                                                                             <  1:2865938/140‑1 (MQ=18)

             aGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGaa                                                                                                                             <  1:6835537/140‑1 (MQ=255)

             aGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGaa                                                                                                                             <  2:6401353/140‑1 (MQ=255)

                   gAGTAACAGGTTTTGCTTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTtct                                                                                                                       <  2:9388128/140‑1 (MQ=17)

                   gAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTtct                                                                                                                       >  1:8756383/1‑140 (MQ=255)

                    aGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCACTAGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTtatc                                                                                                                      >  1:8289946/1‑140 (MQ=17)

                    aGTAACAGGTTTTGATGATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCCGAGCGGCGAGCTTCTCCCTTCTGGTCCATGCCTGAGGCAACGCTCTCTCGCGCGCCCGCCAGGCGGAACTTGGTCGAAGCTtcta                                                                                                                      >  2:5157995/1‑139 (MQ=12)

                      tAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                                    <  1:6743234/140‑1 (MQ=255)

                      tAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                                    <  1:7785517/140‑1 (MQ=255)

                      tAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                                    <  2:815785/140‑1 (MQ=255)

                      tAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                                    <  1:1622429/140‑1 (MQ=255)

                      gaaCAGCGTTTGCTGATTTAAGGCAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                                    <  2:7427630/139‑1 (MQ=14)

                           ggTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCCGCGCTGCGCGCTTCGCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCCTGGTCGAAGCTTCTCCTCCGTc                                                                                                               <  2:1257740/140‑1 (MQ=14)

                           ggTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTc                                                                                                               <  1:7728011/140‑1 (MQ=255)

                           ggTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTc                                                                                                               <  1:5743161/140‑1 (MQ=255)

                              tttGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGTACCTTGGTCGAAGCTTCTCATCCTTcacc                                                                                                            >  2:6503452/1‑140 (MQ=14)

                              tttGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTcccc                                                                                                            >  2:405924/1‑140 (MQ=255)

                              tttGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTcccc                                                                                                            >  2:6909669/1‑140 (MQ=255)

                                tgttgtttttAGGAAGAGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGCCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGc                                                                                                          <  2:8831300/134‑1 (MQ=11)

                                 gtttattTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGACGAAGCTTCTCATCCTTCCCCGCa                                                                                                         <  2:2419474/138‑1 (MQ=11)

                                 gATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGct                                                                                                         <  1:3512246/140‑2 (MQ=255)

                                 gATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGct                                                                                                         <  2:3340253/140‑2 (MQ=255)

                                  attattTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGctt                                                                                                        <  2:7340661/140‑3 (MQ=14)

                                     attTCAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGACCCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                     <  2:5919425/140‑1 (MQ=11)

                                     attTAAGGAAGCGATGGTGGTGGGGGACGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                     <  2:1524939/140‑1 (MQ=14)

                                     aattAAGCAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                     <  1:6416907/138‑1 (MQ=11)

                                      tttAAGTAAGCGATGGTGGTGGGGGAAGGATTAATCAGCGCTGCTCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTATCATCCTTCCCCGCATGGGc                                                                                                    >  2:2071785/1‑140 (MQ=11)

                                      tttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCATTGTCGAAGCATCTCATCCTTCCCCGCATGGGc                                                                                                    >  1:6659669/1‑140 (MQ=11)

                                       ttcaGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                   <  1:4478953/137‑1 (MQ=11)

                                       ttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                   <  2:3228450/140‑1 (MQ=255)

                                       ttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                   <  1:7318204/140‑1 (MQ=11)

                                       ttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                   <  2:5768057/140‑1 (MQ=11)

                                       ttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                   <  2:9527382/140‑1 (MQ=11)

                                       ttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                   <  2:4691315/140‑1 (MQ=11)

                                       ttAAGGAAGCGATGGTGGTGGGGGAAGGAGTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                   <  1:8837640/140‑1 (MQ=11)

                                       ttAAGCACGCGAGGGTGGTGGGGGCAGGAGTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGACGAAGCTTCTCATCCGTCCCCGCATGGGCa                                                                                                   <  1:7007763/140‑1 (MQ=11)

                                       tgaaGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                   <  2:2297364/138‑1 (MQ=11)

                                         aaGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGa                                                                                                 <  1:4120232/140‑1 (MQ=11)

                                                cGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGTCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTg                                                                                          >  1:9585440/1‑140 (MQ=11)

                                                cGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCACTCTCGCAGGCCCGCAAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCCCAAGGGGACAAAAttaa                                                                                          >  2:1172659/1‑138 (MQ=11)

                                                                            cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCgggg                                                              <  1:8232896/140‑1 (MQ=11)

                                                                             gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGc                                                             <  2:8585360/140‑1 (MQ=11)

                                                                                 cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCCAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCGTGGGCAGAATATTTGGTTGCGGGTTCGGTTGTGAGTTCCGGGGCtttt                                                         >  1:7109750/1‑140 (MQ=11)

                                                                                 cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGACGCTTCTCATCCTTCCCCGCTTGGGCAGAATAATTGATTGCGGATTCGTTTGAGAATTCCGGGGCtttt                                                         <  2:4990719/140‑1 (MQ=11)

                                                                                 cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCtttt                                                         >  2:9149987/1‑140 (MQ=11)

                                                                                 cgcgcTTCGCCCTTCGGGTCGTGGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTAGTTTGACAATTCAGGGGcgtat                                                         >  2:6692489/1‑136 (MQ=11)

                                                                                       tCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTGGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGCTTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGACAGt                                                   <  2:5627515/140‑1 (MQ=11)

                                                                                        cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTg                                                  <  1:9768195/140‑1 (MQ=11)

                                                                                        cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTg                                                  <  2:1880647/140‑1 (MQ=11)

                                                                                        cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCCGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTg                                                  >  1:8049491/1‑140 (MQ=11)

                                                                                         gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGa                                                 <  1:3097659/140‑1 (MQ=11)

                                                                                         gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTAAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGCTTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGa                                                 <  1:9170119/140‑1 (MQ=11)

                                                                                           ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGATTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtg                                               <  2:7105084/140‑1 (MQ=11)

                                                                                            cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtgg                                              <  1:5398683/140‑1 (MQ=11)

                                                                                             ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggt                                             <  1:2145339/140‑1 (MQ=11)

                                                                                              tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtg                                            <  2:4224614/140‑1 (MQ=11)

                                                                                               cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtgg                                           <  1:9524141/140‑1 (MQ=11)

                                                                                                gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtggt                                          <  1:5847040/140‑1 (MQ=11)

                                                                                                gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtggt                                          <  1:9399193/140‑1 (MQ=11)

                                                                                                gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtggt                                          <  1:328008/140‑1 (MQ=11)

                                                                                                gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtggt                                          <  1:8621883/140‑1 (MQ=11)

                                                                                                 ggTCGTTGCCTGCGGCAACGCTCTCTCGCGGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtggtg                                         <  2:4862117/140‑1 (MQ=11)

                                                                                                     gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCACGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGACTATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                     <  2:868897/140‑1 (MQ=11)

                                                                                                     gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                     <  2:8398106/140‑1 (MQ=11)

                                                                                                     gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                     <  1:4688198/140‑1 (MQ=11)

                                                                                                     gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                     <  2:3936583/140‑1 (MQ=11)

                                                                                                     gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                     <  1:5059474/140‑1 (MQ=11)

                                                                                                     gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                     <  2:2708882/140‑1 (MQ=11)

                                                                                                       tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCATGGTCGAAGCTTAACATCCTTACCCGCTTTGGCAGAATATTTGATTGCGGATTAGTTTGATAATTACGGGGCTTTTGTAAGTTATGGTGGGGGGGGaa                                   >  2:386204/1‑140 (MQ=11)

                                                                                                          cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                <  1:8176925/140‑1 (MQ=11)

                                                                                                          cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                <  2:2005502/140‑1 (MQ=11)

                                                                                                          cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                <  2:1004748/140‑1 (MQ=11)

                                                                                                          cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                <  2:7885535/140‑1 (MQ=11)

                                                                                                          cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                <  2:2906727/140‑1 (MQ=11)

                                                                                                          cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGAGTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                <  1:6214367/140‑1 (MQ=11)

                                                                                                          cTGCGGCAACGCTCTCTCGCTGGCGCNCAAGTCGNACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAANTGATGTCGGTGGGGGAAGGa                                <  1:6250720/140‑1 (MQ=11)

                                                                                                               gCAACGCTCTCTCGCTGGCGCTCAAGTCGACCCTTGGTCGAAGCGTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACt                           <  2:6104494/140‑1 (MQ=14)

                                                                                                                cAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTc                          >  1:4432476/1‑140 (MQ=14)

                                                                                                                        tctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGACTAGTTGATTGCGGAGTGGTTGGAGACTTCCGGGGCTTTGGAAAGTGATGGTGGTGGGGGCAGGATTACTCAGggcggc                  >  1:378972/1‑140 (MQ=14)

                                                                                                                        tctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCGTCTCACCCTTCCCCGCATGGGCAGACTATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgc                  <  1:1401650/140‑1 (MQ=11)

                                                                                                                        gctcGCTGGCGCTCACGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGCTTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgc                  <  2:255289/139‑1 (MQ=14)

                                                                                                                           cGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcg               <  1:2328386/140‑1 (MQ=14)

                                                                                                                              tGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCtt            <  2:2770678/140‑1 (MQ=14)

                                                                                                                               gacgcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTc           >  2:5673622/3‑140 (MQ=11)

                                                                                                                                gcgcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATCTTTGATTGCGGATTCGTGTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg          <  2:7588148/140‑1 (MQ=14)

                                                                                                                                gcgcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg          >  2:5489888/1‑140 (MQ=14)

                                                                                                                                acgcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATCTGTGATTGCGGATTCGCGTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGGGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg          >  1:6576681/2‑140 (MQ=11)

                                                                                                                                acgcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg          >  1:4778442/2‑140 (MQ=255)

                                                                                                                                 cgcTCTAGGCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGATTATTTGATTGCGGATTCGATTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGc         <  1:4288376/140‑1 (MQ=11)

                                                                                                                                  gcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAANGATTACTCAGCGCGGCGCGCTTCGcc        >  1:7147665/1‑140 (MQ=11)

                                                                                                                                   ctcctgCCGACCGTTGATCGTATCGTCTGACCCTTCACCGAATTGGCAGAATAATTTATTGCGTCTTAGCTTGAGATTTCCCGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGccc       <  1:1841270/135‑1 (MQ=9)

                                                                                                                                    tcctcccGAACCTGCCTCCAAGCTTCTGACCCTTACCCGCCTGGGCATACTACTTGATTGCCGATTCGCTAGAGTCTTCACTGACTTTTGAAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCt      <  1:8656132/135‑1 (MQ=2)

ATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGG  >  NZ_CP025268/1292676‑1292951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`