BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,293,001	A→G	noncoding (36/132 nt)	CXP41_RS06835 ←	RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,293,001	0	A	G	99.6%	778.3 / NA	277	noncoding (36/132 nt)	CXP41_RS06835	RtT sRNA
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (121/155); minor base T (1/0); total (122/155)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

TGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGT  >  NZ_CP025268/1292866‑1293134

tGCGGATTCGTTTGAGCATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                   <  2:8663259/140‑1 (MQ=255)

tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGACGAACCTTAGTCGa                                                                                                                                   >  2:2016561/1‑140 (MQ=255)

tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                   <  2:8625600/140‑1 (MQ=255)

tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                   >  1:6805517/1‑140 (MQ=255)

tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATAACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAAACTTAGTCGa                                                                                                                                   >  2:7793121/1‑140 (MQ=255)

 gCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGaa                                                                                                                                  <  2:3832208/140‑1 (MQ=255)

 gCGGAGTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCCGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCTTAGTCGaa                                                                                                                                  <  2:8973796/140‑1 (MQ=14)

  cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCAGCGGCAACGCACTCAAGCTGAAGCTCGAGCCGAACCTTAGTCGAAg                                                                                                                                 >  1:2411678/1‑140 (MQ=14)

  cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAg                                                                                                                                 <  1:5374508/140‑1 (MQ=255)

    gATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCt                                                                                                                               <  1:146852/140‑1 (MQ=255)

    gATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCt                                                                                                                               >  1:9483961/1‑140 (MQ=255)

      ttCGTTTGAGAATTCCGGGGATTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTtc                                                                                                                             <  1:7112100/140‑1 (MQ=255)

               gcaTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTc                                                                                                                    <  1:7568985/138‑1 (MQ=255)

               gaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTc                                                                                                                    <  2:123502/140‑1 (MQ=255)

               gaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGACGCGCGAGCCGGACCTTAGTCGAAGCTTCTCATCCTTc                                                                                                                    >  1:2914260/1‑140 (MQ=255)

                    ccgtgtcTTTTGCAAGAGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGc                                                                                                               <  2:5030827/134‑1 (MQ=11)

                    ccGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGc                                                                                                               <  1:7727267/140‑1 (MQ=37)

                    ccGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGc                                                                                                               <  1:7609576/140‑1 (MQ=37)

                     cGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCa                                                                                                              <  2:9456876/140‑1 (MQ=25)

                     cGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCa                                                                                                              <  1:2011012/140‑1 (MQ=25)

                         tcTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                          <  2:8396392/139‑1 (MQ=12)

                         tatttaGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                          <  1:4174867/138‑1 (MQ=12)

                           ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                        <  1:9012057/140‑1 (MQ=17)

                            tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTCTCGCAGACCCTCGAGCCGAACCTTAGTCGAAGCTTAACATCCATCCCCGCAAGGGCAg                                                                                                       >  1:9337980/1‑140 (MQ=11)

                             ttGTAAGTGTTGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                                      <  1:8981614/140‑1 (MQ=12)

                                    tGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCTCCCTTCGGGTCGTGGCCTGCGGCAACGCTCACTCCCTGACGCACGAGGCGAAACTTAGTAGAATCTTCTAATACTTCCCCCCATGGGCACaaaaattg                                                                                               >  1:801873/1‑134 (MQ=11)

                                      aTGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAAGGGCAGAaaataagat                                                                                             >  2:4652271/1‑135 (MQ=11)

                                                   aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCACGAGCCGAACCTTAGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAAAATTTAATTGCGGATTCGGTg                                                                                >  2:2731098/1‑140 (MQ=11)

                                                    aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                               >  2:3499933/1‑140 (MQ=11)

                                                    aGGATTACTCAGCGCTGCGCGCATCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGGAGAATATTGAATTGCGGATTCGTTgg                                                                               >  2:5952012/1‑140 (MQ=11)

                                                      tttttcTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                             <  1:6947948/135‑1 (MQ=11)

                                                      gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                             <  1:2317282/140‑1 (MQ=255)

                                                      gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                             <  2:7162827/140‑1 (MQ=255)

                                                      gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                             <  1:8157887/140‑1 (MQ=255)

                                                      gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                             <  1:7793121/140‑1 (MQ=255)

                                                      gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                             <  2:7859615/140‑1 (MQ=255)

                                                      gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                             >  2:7905413/1‑140 (MQ=255)

                                                      gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCGGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCATCCCCGCATGGGCCGACAATTTAATTTGGGGTTCGGTgggg                                                                             >  1:3481637/1‑139 (MQ=11)

                                                           cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCCACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGCTGGGGAGttg                                                                        >  2:5371787/1‑139 (MQ=11)

                                                           cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGCATATTTAATTGAGGATTCGTTGGGCAGTTc                                                                        >  2:2225288/1‑140 (MQ=11)

                                                               gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTAGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAggg                                                                    <  1:3908714/140‑1 (MQ=14)

                                                               gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAggg                                                                    <  1:3278490/140‑1 (MQ=255)

                                                               gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCCAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAggg                                                                    <  1:8066515/140‑1 (MQ=14)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                   >  2:4628559/1‑140 (MQ=255)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGAATATTTAATTTCGGATTAGTTGGGGAGGTGAgggg                                                                   >  2:1035403/1‑139 (MQ=11)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCGCTCGCTGGCGCTCAAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCTGGGGCCGAATATTTAATTTCGGATTCGGTGGGAAGTTCAGGGa                                                                   >  2:8066515/1‑140 (MQ=11)

                                                                 gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGAc                                                                  <  1:3282588/140‑1 (MQ=255)

                                                                 gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGAc                                                                  >  2:209928/1‑140 (MQ=255)

                                                                   tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtt                                                                >  2:5844167/1‑140 (MQ=255)

                                                                   tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCGCGATTCGAACCTGGGGGGAAGCTTGTCTTCCTTCCCCGCTGGGGGAGACTATTTAATTGCGGGTTGGGTGGGACGTTCAgggccgg                                                                >  2:3210267/1‑136 (MQ=2)

                                                                   tGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCGCTAGCGCGGGCGCTCCAGTCGTACCTTGGTCGAAGCTTCTCAACCTTACCCGCATGGGCAGAATATATAATTTCCGAGTCGTTGGAAACTACAGGGACtg                                                                >  2:6494402/1‑139 (MQ=2)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTTCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTGGTTGGGAAGTTCAGGGACtttt                                                              >  2:557220/1‑140 (MQ=14)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTTGCGCTCGAGTCGAACCTTGGTCGAAGGTTCTCATCCTTCCCCGCATGGGCCGAAAATTTAATAGCGGATACGTGGGGAAGATAAgggtggttg                                                              >  2:6190745/1‑134 (MQ=11)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAggg                                                                    >  2:8823086/1‑134 (MQ=255)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAggg                                                                    <  1:8823086/134‑1 (MQ=255)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                              >  2:8923328/1‑140 (MQ=255)

                                                                     cgcgcATCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCCGAATATTTAATTGCGGAGTCGTTGGGAAGTTCAGGGACtttt                                                              >  2:7730059/1‑140 (MQ=14)

                                                                        gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                           <  1:5490087/140‑1 (MQ=255)

                                                                        gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCTTTGGGAAGTTCAGGGACTTTTGaa                                                           <  1:2071785/140‑1 (MQ=14)

                                                                         cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGGAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                          <  2:2965294/140‑1 (MQ=14)

                                                                         cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                          <  1:1037859/140‑1 (MQ=255)

                                                                          ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAg                                                         <  1:4952807/140‑1 (MQ=255)

                                                                          ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGAATATTTCATTGCGGATTCGGTGGGGAGTTCAGGGACTTTTGAAAg                                                         >  2:1257625/1‑140 (MQ=11)

                                                                          ttCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGCGTCGAACCTGGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGAGTCGTTGGGAAGTTCAGGGACTTTTGAAAg                                                         >  2:5989833/1‑140 (MQ=14)

                                                                          ttCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATACTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGatat                                                         >  2:6760151/1‑137 (MQ=14)

                                                                          ttCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGACGCTTCTCATCCTTCCCCGCAGGGGCCGTCTATTTAATTGCGGAGGCGTGGGGACGTTCAGGGAGGTTTGTAAg                                                         >  2:9709108/1‑140 (MQ=2)

                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGGTGGCGCTCGAGTCGAACCTTTGTCGTAGATTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGA                                                      <  1:9635904‑M1/140‑1 (MQ=255)

                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGA                                                      <  1:1212337‑M1/140‑1 (MQ=255)

                                                                              cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAT                                                     <  2:4134422‑M1/140‑1 (MQ=255)

                                                                              cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAT                                                     <  1:3128036‑M1/140‑1 (MQ=255)

                                                                                  tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTG                                                 <  2:7600975‑M1/140‑1 (MQ=255)

                                                                                   cGGGTCGTTGCCTGCGGCATCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGG                                                <  2:2843554‑M1/140‑1 (MQ=255)

                                                                                   cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGG                                                <  2:7344815‑M1/140‑1 (MQ=255)

                                                                                   cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTACCCGCATGGGCAGACTATTTAATTGCGGATTCGTTGGGAAGTTGAGGGACTTTTGAAAGTGATGGTGG                                                >  2:7442691‑M1/1‑140 (MQ=255)

                                                                                          ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                                         <  1:5160175‑M1/140‑1 (MQ=255)

                                                                                          gtgCCTGCGGCAACGCTCTCTCGCTGGAGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                                         <  2:951862‑M1/139‑1 (MQ=255)

                                                                                           tGCCTGCGGCAACGCTCTCTCTCTGGCGCTAGAGTCGAACCTTGGTCGAAGCTTCTCATCGTTACCAGAATTTGATGAATATTTTATTGCGGATTCGTTGGTTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                        <  1:1747446/140‑1 (MQ=11)

                                                                                           tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAA                                        <  1:1591522‑M1/140‑1 (MQ=255)

                                                                                           tGCCTGCGGCAACGCTCTCTCGATGGCGCTCAAGTCGAACCTTTGTCTAATCTTCTAATCCTTCCCCGCATGGGCATAATATTTAAATGCGGATTAGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTTGTGGGGGAA                                        >  2:1722344‑M1/1‑140 (MQ=255)

                                                                                                gCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATT                                   <  2:1938837‑M1/140‑1 (MQ=255)

                                                                                                   gCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcga                                <  2:2699443‑M1/140‑4 (MQ=255)

                                                                                                    cAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaa                               <  2:912450‑M1/140‑5 (MQ=255)

                                                                                                          tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAGGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcg                         <  2:6805517‑M1/140‑11 (MQ=255)

                                                                                                            tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGGGATGNTGGTGGGGGCAGGATTcgaaccttcgaa                       >  1:6286745‑M1/1‑128 (MQ=255)

                                                                                                            tctcGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACGTTTGAAAGTGATGGGGGGGGGGGAAGGATTcgaaccttcgaa                       >  1:1257693‑M1/1‑128 (MQ=255)

                                                                                                              tcGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGtt                                                                                 <  1:603560/80‑1 (MQ=11)

                                                                                                              tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCAGGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCTGGGACTTTTGAAAGTGATGGTGGTGGGGGACGGATTcgaaccttcgatgt                     >  1:8405427‑M1/1‑126 (MQ=255)

                                                                                                                  tggcgctCGAGTCGAACCATGGTCGAATCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccatcgaagtcgat                 >  2:2491575‑M1/4‑122 (MQ=255)

                                                                                                                   ggcgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaacttcgaagtcgatg                >  2:9311671‑M1/3‑121 (MQ=255)

                                                                                                                    gcgCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatga               >  1:255684‑M1/2‑120 (MQ=255)

                                                                                                                     cGCTCGAGTCGAACCTTCGCCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGGTGGGAAGTTCTGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgac              <  1:8705516‑M1/140‑22 (MQ=255)

                                                                                                                       cTCGAGTCGATCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacgg            <  2:1481587‑M1/140‑24 (MQ=255)

                                                                                                                       cTCGAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTACTTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacgg            <  2:9370396‑M1/140‑24 (MQ=255)

                                                                                                                       cTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacgg            <  2:3731456‑M1/140‑24 (MQ=255)

                                                                                                                        tCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCACGCATGGGCAGAATATTTAATTGCGGAGTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggc           <  2:3412137‑M1/140‑25 (MQ=255)

                                                                                                                         cGAGTCGAACCTTGGTCGACGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggca          <  1:7402094‑M1/140‑26 (MQ=255)

                                                                                                                              cGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggcagattt     >  1:6477041‑M1/1‑110 (MQ=255)

                                                                                                                              cGAACCTTGGTCGAAGCTTCTAATCCTACCCCGCATGGGCAGAATATTTAATTGCGGATTATTTTGTAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGTATTctaacctacgaagacgattacggcagattt     >  2:7094031‑M1/1‑110 (MQ=255)

                                                                                                                               gAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATATAATGGCGGATTCGTTGGGACGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggcagattta    <  2:8004330‑M1/140‑32 (MQ=255)

                                                                                                                                 aCCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggcagatttaca  <  1:2242102‑M1/140‑34 (MQ=255)

TGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGT  >  NZ_CP025268/1292866‑1293134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`