BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	155,766	C→T	K89K (AAG→AAA)	yadV ←	fimbrial chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	155,766	0	C	T	99.2%	1485.5 / NA	393	K89K (AAG→AAA)	yadV	fimbrial chaperone
Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (131/258); minor base A (0/3); total (131/262)
Fisher's exact test for biased strand distribution p-value = 5.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 394 total aligned reads displayed.

TGCAGCAGGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTA  >  NZ_CP025268/155629‑155899

   agcagGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGc                                                                                                                                  <  2:9151931/140‑1 (MQ=255)

   agcagGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGc                                                                                                                                  <  1:349633/140‑1 (MQ=255)

   agcagGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGc                                                                                                                                  <  1:1880177/140‑1 (MQ=255)

   agcagGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGc                                                                                                                                  <  1:6970419/140‑1 (MQ=255)

   agcagGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGc                                                                                                                                  <  1:7329963/140‑1 (MQ=255)

     cagGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCAt                                                                                                                                <  2:2809190/140‑1 (MQ=255)

          tttGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATa                                                                                                                           <  2:177597/140‑1 (MQ=255)

          tttGATTCGCGACCTTGTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATa                                                                                                                           <  2:6956347/140‑1 (MQ=255)

            tGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATAcg                                                                                                                         <  2:4354350/140‑1 (MQ=255)

             gATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATAcgc                                                                                                                        <  2:2572862/140‑1 (MQ=255)

                  gcgACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATAc                                                                                                                   >  1:7877307/1‑140 (MQ=255)

                  gcgACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATAc                                                                                                                   >  2:8502878/1‑140 (MQ=255)

                  gcgACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGAGAGGTGCTGGCGGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGAtcc                                                                                                                   <  2:846634/140‑3 (MQ=255)

                   cgACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACt                                                                                                                  >  2:127557/1‑140 (MQ=255)

                     aCCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTgg                                                                                                                <  1:9550816/140‑1 (MQ=255)

                        ttttCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCTCACGTTTGGCATCAATACGCGATACTggcgg                                                                                                             >  1:4082302/1‑140 (MQ=255)

                           tCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAACCCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTNTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGt                                                                                                          <  1:6316145/140‑1 (MQ=255)

                           tCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGt                                                                                                          <  1:9019019/140‑1 (MQ=255)

                            ccgCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCACTACGCGATACTGGCGGCGTa                                                                                                         <  2:134326/138‑1 (MQ=255)

                            cTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTa                                                                                                         <  1:5952514/140‑1 (MQ=255)

                             tGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                        <  1:2371569/140‑1 (MQ=255)

                             tGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                        <  1:6609963/140‑1 (MQ=255)

                               cATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCa                                                                                                      <  1:7052094/140‑1 (MQ=255)

                               cATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCa                                                                                                      <  2:9725443/140‑1 (MQ=255)

                                aTCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCa                                                                                                                              >  1:3749920/1‑115 (MQ=255)

                                aTCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAg                                                                                                     >  2:3975045/1‑140 (MQ=255)

                                    ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaa                                                                                                 >  1:5496279/1‑140 (MQ=255)

                                    ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaa                                                                                                 >  2:5898699/1‑140 (MQ=255)

                                    ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaa                                                                                                 <  1:6886133/140‑1 (MQ=255)

                                    ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaa                                                                                                 >  2:733093/1‑140 (MQ=255)

                                    ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaa                                                                                                 >  1:8091499/1‑140 (MQ=255)

                                    ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaa                                                                                                 <  1:2192102/140‑1 (MQ=255)

                                     gTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaaa                                                                                                <  2:4739686/140‑1 (MQ=255)

                                       tttGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTTTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                              <  2:7720239/140‑1 (MQ=255)

                                         tggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTTGGCATTGAGGTGCTGGCTGTGTACATTAATTTGTTTGTTTGCCCACGTTTGGCATCAATACGCGATTCGGGCGGCGTAGCTGTAAATGGGa                                                                                            <  2:5240274/140‑1 (MQ=255)

                                         tggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGa                                                                                            <  1:8601975/140‑1 (MQ=255)

                                               aaCTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTaa                                                                                      <  2:6511385/140‑1 (MQ=255)

                                               aaCTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTaa                                                                                      <  1:7254186/140‑1 (MQ=255)

                                                     cAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGc                                                                                >  2:4104851/1‑140 (MQ=255)

                                                      aGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGcc                                                                               <  1:1269007/140‑1 (MQ=255)

                                                      aGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGcc                                                                               <  1:6163297/140‑1 (MQ=255)

                                                      aGTACGAGAACCCAGACCTCGCTCTCTCAGTCATTAGGCAGAGAGGTGCTGGCTGTGTACATTAATTTGATTGTGTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGcc                                                                               <  2:6893754/140‑1 (MQ=255)

                                                            ttAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTc                                                                         >  2:491764/1‑140 (MQ=255)

                                                             tAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCa                                                                        >  2:1498557/1‑140 (MQ=255)

                                                                aCCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCg                                                                     <  2:8881871/140‑1 (MQ=255)

                                                                aCCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCg                                                                     <  2:1134244/140‑1 (MQ=255)

                                                                     aaCACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTc                                                                >  1:3844278/1‑140 (MQ=255)

                                                                     aaCACGCTCTCTCTGTCTTTAGGCAGTGAAGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTc                                                                <  1:9680954/140‑1 (MQ=255)

                                                                      acacGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCa                                                               <  2:4236559/140‑1 (MQ=255)

                                                                          gCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGc                                                           >  1:1025220/1‑140 (MQ=255)

                                                                          gCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGc                                                           <  2:5013778/140‑1 (MQ=255)

                                                                          gCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGc                                                           >  1:6758126/1‑140 (MQ=255)

                                                                          gCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGc                                                           <  1:2627861/140‑1 (MQ=255)

                                                                           ctctctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGcc                                                          <  2:9510885/140‑1 (MQ=255)

                                                                           ctctctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGcc                                                          <  1:5068245/140‑1 (MQ=255)

                                                                              tctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTTATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGt                                                       <  2:228781/140‑1 (MQ=255)

                                                                              tctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGt                                                       <  1:7798830/140‑1 (MQ=255)

                                                                              tctctGTCTTTAGGCAGTGAGGTGCGGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGt                                                       <  1:3132408/140‑1 (MQ=255)

                                                                                tctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTACTTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTAt                                                     <  2:2058173/140‑1 (MQ=255)

                                                                                tctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTAt                                                     <  1:2048804/140‑1 (MQ=255)

                                                                                tctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAAGACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTAt                                                     <  2:3412350/140‑1 (MQ=255)

                                                                                    tCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTaa                                                 >  1:4258253/1‑140 (MQ=255)

                                                                                     cTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAAc                                                <  1:5694672/140‑1 (MQ=255)

                                                                                     cTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAAc                                                <  2:6233198/140‑1 (MQ=255)

                                                                                     cTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAAc                                                <  2:4509194/140‑1 (MQ=255)

                                                                                     cTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAAc                                                <  1:1357260/140‑1 (MQ=255)

                                                                                      tttAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAAcc                                               <  2:8173138/140‑1 (MQ=255)

                                                                                      tttAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAAcc                                               <  1:8679746/140‑1 (MQ=255)

                                                                                          ggCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAAct                                           >  1:9695871/1‑140 (MQ=255)

                                                                                          ggCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAAct                                           >  1:8371007/1‑140 (MQ=255)

                                                                                          ggCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAAct                                           <  1:3248713/140‑1 (MQ=255)

                                                                                              gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTgg                                       >  2:7405744/1‑140 (MQ=255)

                                                                                               tGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGa                                      <  2:3788242/140‑1 (MQ=255)

                                                                                                gAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTGGGAATCTATACGCGATACTGGCGGCGTAGCAGTAAAAGGTACTGTAATACTGCCAGGCTCAGCGTTGTCTTCGCCAGTATCTAACCAACTCTGGAc                                     <  2:9358888/140‑1 (MQ=255)

                                                                                                 aGGTGCTGGCTGTGTACAATAATTTGATTGTTTGCCCACGATTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACa                                    <  2:6464497/140‑1 (MQ=255)

                                                                                                     gctggctgTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCa                                <  1:2897451/140‑1 (MQ=255)

                                                                                                     gctggctgTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCa                                <  2:4589739/140‑1 (MQ=255)

                                                                                                         gctgTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAg                                                                       <  2:7544171/97‑1 (MQ=255)

                                                                                                         gctgTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAg                                                                       >  1:7544171/1‑97 (MQ=255)

                                                                                                           tgtgTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGt                          >  2:3562957/1‑140 (MQ=255)

                                                                                                            gtgtACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGtt                         <  1:1073558/140‑1 (MQ=255)

                                                                                                            gtgtACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGAGACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGtt                         <  2:6672991/140‑1 (MQ=255)

                                                                                                              gtACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTAt                       >  1:8246075/1‑140 (MQ=255)

                                                                                                                 cATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTcc                    <  1:456672/140‑1 (MQ=255)

                                                                                                                   ttAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCt                  >  1:9382848/1‑140 (MQ=255)

                                                                                                                   ttAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCt                  >  1:5120068/1‑140 (MQ=255)

                                                                                                                       tttGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTc                                                                         <  1:2562318/81‑1 (MQ=255)

                                                                                                                       tttGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTAt              >  1:5275663/1‑140 (MQ=255)

                                                                                                                       tttGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTAt              >  2:5710522/1‑140 (MQ=255)

                                                                                                                       tttGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTAt              >  1:4756741/1‑140 (MQ=255)

                                                                                                                          gATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTAtttt           <  1:3079180/140‑1 (MQ=255)

                                                                                                                           aTTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTTCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTc          >  2:7036785/1‑140 (MQ=255)

                                                                                                                           aTTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTc          >  2:6922115/1‑140 (MQ=255)

                                                                                                                           aTTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTc          <  1:5875993/140‑1 (MQ=255)

                                                                                                                           aTTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTc          <  1:1706319/140‑1 (MQ=255)

                                                                                                                           aTTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTc          <  2:3709112/140‑1 (MQ=255)

                                                                                                                           aTTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTc          <  1:4675401/140‑1 (MQ=255)

                                                                                                                             tGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCa        <  2:7866198/140‑1 (MQ=255)

                                                                                                                               tttGCCCACTTTTGGCATCAATTCGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTATTACTGCCAGGCTCTGCGTTGTCATCGCCAGTATCTAACCAACTCTTGACAAGCAACGGGTTATTCCCTTTATTTTCCAGa      <  2:3080396/140‑1 (MQ=255)

                                                                                                                               tttGCCCACGTTTGGCATCAATCCGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCCACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGa      <  2:5391825/140‑1 (MQ=255)

TGCAGCAGGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTA  >  NZ_CP025268/155629‑155899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`