BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,660,166	T→C	K109R (AAG→AGG)	ynfA ←	YnfA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,660,166	0	T	C	98.7%	1610.0 / NA	468	K109R (AAG→AGG)	ynfA	YnfA family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (221/241); minor base A (5/1); total (226/242)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 468 total aligned reads displayed.

TAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑CC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCAC  >  NZ_CP025268/1660029‑1660301

taAAGATCGAAAAAAGTCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTa                                                                                                                                          >  1:1082618/1‑140 (MQ=255)

taAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATCAAATGGATTTATGTTCCTa                                                                                                                                          >  2:6403273/1‑140 (MQ=255)

taAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTa                                                                                                                                          >  1:1979564/1‑140 (MQ=255)

taAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTa                                                                                                                                          >  2:4209606/1‑140 (MQ=255)

taAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTa                                                                                                                                          >  1:2474915/1‑140 (MQ=255)

       cGAAAAAAGCCGGAGTGATCACAAAAAAAGTCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGTATACCTTATGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCATAAGTgctc                                                                                                                                   >  2:9065836/1‑138 (MQ=255)

        gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑cc                                                                                                                                 >  1:965414/1‑140 (MQ=255)

        gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑cc                                                                                                                                 >  1:5952090/1‑140 (MQ=255)

        gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑cc                                                                                                                                 >  1:7102673/1‑140 (MQ=255)

        gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAAAAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑cc                                                                                                                                 >  1:8021036/1‑140 (MQ=255)

         aaaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATATTAATGATAAAATGGATTGATGTTCCTACGTGCG‑cc‑c                                                                                                                               >  2:885121/1‑140 (MQ=255)

                 cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCg                                                                                                                       <  1:5879772/140‑1 (MQ=255)

                   gAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGca                                                                                                                     >  2:3076989/1‑140 (MQ=255)

                      tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGcaaca                                                                                                                  <  2:8478400/140‑1 (MQ=255)

                        aTCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAAt                                                                                                                <  1:6115417/140‑1 (MQ=255)

                           acaAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGAt                                                                                                             <  1:4895176/140‑1 (MQ=255)

                             aaaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCa                                                                                                           <  1:9726145/140‑1 (MQ=255)

                             aaaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCa                                                                                                           <  1:6875801/140‑1 (MQ=255)

                              aaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCaa                                                                                                          >  2:5184554/1‑140 (MQ=255)

                               aaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAAc                                                                                                         <  2:1676659/140‑1 (MQ=255)

                                       gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCa                                                                                                 >  1:3000913/1‑140 (MQ=255)

                                        tataTTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCaa                                                                                                <  1:9153786/140‑1 (MQ=255)

                                          tatTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAg                                                                                              <  2:4794144/140‑1 (MQ=255)

                                                cgcTGAGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                        >  2:9742487/1‑140 (MQ=255)

                                                   tgtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCaa                                                                                     <  2:5252215/140‑1 (MQ=255)

                                                   tgtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCaa                                                                                     >  2:5784955/1‑140 (MQ=255)

                                                   tgtgAATGGTTGACAAAAGATGAAATAGAATACCTTTGGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGGTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCaa                                                                                     <  2:8692549/140‑1 (MQ=255)

                                                   agtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCaa                                                                                     <  1:1545663/139‑1 (MQ=255)

                                                    gtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAAc                                                                                    >  2:2222699/1‑140 (MQ=255)

                                                    gtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAAc                                                                                    >  2:2104139/1‑140 (MQ=255)

                                                     ttaaTGGTTTACAAAATATGAAATAGAATACATTTTGTCAGCTGACACTTCCTCTTATCTTATTGATTAATTGGATTTATGTTCCTACGTGCGACC‑C‑CTGCACGCTACAATGATAAACATGCCGCAAAGCGCAATCTACg                                                                                   <  2:2912896/138‑1 (MQ=255)

                                                     tgAATGGTTGACAAAAGATGAAATAGACTACCTTTTGTCAGCTGACACTTCCTCGTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                   <  2:3442015/140‑1 (MQ=255)

                                                     tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                   >  1:1009403/1‑140 (MQ=255)

                                                       aaTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                                 >  2:101873/1‑140 (MQ=255)

                                                        aTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTCTTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                <  2:3273047/140‑1 (MQ=255)

                                                           gTTGACAAAAGATGAAATAGCATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCg                                                                             <  1:8962579/140‑1 (MQ=255)

                                                             ttaCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  2:4356718/138‑1 (MQ=255)

                                                             tGACCAAAGATGCCACAGAATACCATTTGTCAGCTGACACGTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  1:2123526/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  1:6933089/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  1:7433311/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  2:5381589/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCATATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  1:4503762/140‑1 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCACAGCGCAATCAACGCACCCGTCCa                                                                         >  2:7323762/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                         >  1:3542858/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                         >  2:1204220/1‑140 (MQ=255)

                                                                 aaaaGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGt                                                                       <  1:3211266/140‑1 (MQ=255)

                                                                 aaaaGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGt                                                                       >  1:2689794/1‑140 (MQ=255)

                                                                     gATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATa                                                                   <  1:4745678/140‑1 (MQ=255)

                                                                      aTGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATaa                                                                  >  1:2376519/1‑140 (MQ=255)

                                                                      aTGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATaa                                                                  >  1:1270742/1‑140 (MQ=255)

                                                                       tGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATaaa                                                                 <  1:6001197/140‑1 (MQ=255)

                                                                       tGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATaaa                                                                 <  2:6828773/140‑1 (MQ=255)

                                                                          aaTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAagag                                                              >  1:5101892/1‑140 (MQ=255)

                                                                           aTAGCATACCTTTTGTCAGCTGACACTTCCTCTTATCTTCTTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                             <  2:8014535/140‑1 (MQ=255)

                                                                               aaTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACCATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGACAGt                                                         >  2:2417139/1‑140 (MQ=255)

                                                                               aaTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGt                                                         >  1:1923205/1‑140 (MQ=255)

                                                                               aaTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGt                                                         >  1:8434481/1‑140 (MQ=255)

                                                                               aaTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGt                                                         >  1:1687846/1‑140 (MQ=255)

                                                                                 tACCTTTTGTCAGCTGACACTTCCTATTATATTATGGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAAAGATCAACATGCCGCAAAGAGCAATCAAAGCTCCCGTCCAGACATAACGAGGCAGttt                                                       >  2:6991919/1‑140 (MQ=255)

                                                                                   ccTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCa                                                     >  1:3489637/1‑140 (MQ=255)

                                                                                    cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCAc                                                    >  1:4367265/1‑140 (MQ=255)

                                                                                     ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                   <  2:7248288/140‑1 (MQ=255)

                                                                                     ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                   <  1:5453489/140‑1 (MQ=255)

                                                                                     ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                   <  1:7628586/140‑1 (MQ=255)

                                                                                       ttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGcc                                                 <  1:8684457/140‑1 (MQ=255)

                                                                                            aGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCca                                            <  2:2746989/140‑1 (MQ=255)

                                                                                              cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCACTCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                          >  2:7560639/1‑140 (MQ=255)

                                                                                              cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCCTCcaca                                          >  2:8286910/1‑140 (MQ=255)

                                                                                                  cacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgc                                      <  1:7454099/140‑1 (MQ=255)

                                                                                                  cacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgc                                      <  2:591212/140‑1 (MQ=255)

                                                                                                    cctCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACgcgc                                    >  2:9340531/3‑140 (MQ=255)

                                                                                                    cTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACgcgc                                    >  2:3873278/1‑140 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                 <  1:8852023/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                 >  1:5786749/1‑140 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                 <  1:3948973/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGGTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAACGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                 <  2:8610380/140‑1 (MQ=255)

                                                                                                       ccTCTAATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CCGCCCGCAACACTGATCAACCTGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCCCAACGCGCAGc                                 <  2:4945545/140‑1 (MQ=255)

                                                                                                        ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGcc                                >  2:542268/1‑140 (MQ=255)

                                                                                                        ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGcc                                >  2:3985012/1‑140 (MQ=255)

                                                                                                        ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGcc                                >  1:2012083/1‑140 (MQ=255)

                                                                                                        ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑CC‑CACAGCCCGCAAAAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCTCGCCATCCAAAACGCGCAGcc                                >  2:8555647/1‑140 (MQ=255)

                                                                                                            tatcttatTGATAAAATGGATTTATGTTCCCACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACAt                            <  2:4472523/140‑1 (MQ=255)

                                                                                                             atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                           >  1:4128444/1‑140 (MQ=255)

                                                                                                             atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                           >  2:3510573/1‑140 (MQ=255)

                                                                                                               cttatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCaa                         >  2:6149492/1‑140 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                       <  1:9427893/140‑1 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                       <  2:3631674/140‑1 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                       <  1:5242796/140‑1 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                       <  1:2595646/140‑1 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                      <  2:6680463/140‑1 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                      >  1:6220640/1‑140 (MQ=255)

                                                                                                                   ttGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                     >  2:1806989/1‑140 (MQ=255)

                                                                                                                   ttGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                     >  2:1626427/1‑140 (MQ=255)

                                                                                                                   ttGATAAAATGGATATATGTTCATACGTGCG‑CCAC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                     >  1:8229563/1‑140 (MQ=255)

                                                                                                                      aTAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                  <  1:657273/140‑1 (MQ=255)

                                                                                                                      aTAAAATCGATTTCTGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                  <  2:7458109/140‑1 (MQ=255)

                                                                                                                        aaaaTGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGAAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa                <  1:5620309/140‑1 (MQ=255)

                                                                                                                                  tctGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCCTCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg      <  2:6542253/138‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg      <  1:8584542/140‑1 (MQ=255)

                                                                                                                                    tGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGcc    <  2:6348391/140‑1 (MQ=255)

TAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑CC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCAC  >  NZ_CP025268/1660029‑1660301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`