BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,186,098	A→G	322 (TAA→TAG) L241L (TTA→CTA)	yegV → yegW ←	carbohydrate kinase family protein GntR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,186,098	0	A	G	99.4%	1832.8 / NA	531	322 (TAA→TAG) L241L (TTA→CTA)	yegV yegW	carbohydrate kinase family protein GntR family transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (252/276); minor base T (2/1); total (254/277)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 531 total aligned reads displayed.

GCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCG  >  NZ_CP025268/2185961‑2186234

gCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGAt                                                                                                                                        <  2:2510614/140‑1 (MQ=255)

gCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGAt                                                                                                                                        <  2:4430346/140‑1 (MQ=255)

gCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGAt                                                                                                                                        <  1:2950339/140‑1 (MQ=255)

  cGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCATTGCAGTGGCGTCGTGGTTTGGCGGGCATCGGGGCGGTGAGTGGGCGCCAACGCGCGAGGACCGACTCCTCGCAAACCAAAACGTATTGATCg                                                                                                                                      >  2:9465230/1‑140 (MQ=255)

  cGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCg                                                                                                                                      <  2:2429083/140‑1 (MQ=255)

      cTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGc                                                                                                                                  >  1:1748124/1‑140 (MQ=255)

          ccTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACa                                                                                                                              <  1:9348556/140‑1 (MQ=255)

          ccTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACa                                                                                                                              <  2:9191817/140‑1 (MQ=255)

          ccTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACa                                                                                                                              <  2:6378900/140‑1 (MQ=255)

          ccTCTGGTCTGCCACTGGCGGAGGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACa                                                                                                                              <  2:6275168/140‑1 (MQ=255)

              tGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGGCGGGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGACCTACTCCTCGCACACCCAATCGTAGAGATCGCTGCGACCGTgg                                                                                                                          >  2:6997504/1‑140 (MQ=255)

               ggTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGCTCGCTGCGACAGTGGc                                                                                                                         <  2:9065801/140‑1 (MQ=255)

               ggTCTGCCACTGGCGGATGCCGTATGACTGGGCAATGCAGTGGCGTCGTGGGTGGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGc                                                                                                                         <  2:5894935/140‑1 (MQ=255)

                 tCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTa                                                                                                                       <  1:2478498/140‑1 (MQ=255)

                   tGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAAt                                                                                                                     >  1:2747906/1‑140 (MQ=255)

                    gCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATg                                                                                                                    <  2:2371924/140‑1 (MQ=255)

                    gCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATg                                                                                                                    <  1:6161261/140‑1 (MQ=255)

                    gCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATg                                                                                                                    <  2:2755334/140‑1 (MQ=255)

                    gCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATg                                                                                                                    <  1:5775708/140‑1 (MQ=255)

                    gCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATg                                                                                                                    <  2:1961920/140‑1 (MQ=255)

                    gCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATg                                                                                                                    >  1:7693316/1‑140 (MQ=255)

                    gCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATg                                                                                                                    <  2:8692974/140‑1 (MQ=255)

                    gCCACAGGCGGATGCCGAATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATg                                                                                                                    <  1:6551696/140‑1 (MQ=255)

                     ccACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACa                                                                                                                              >  1:337683/1‑129 (MQ=255)

                     ccACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGc                                                                                                                   >  2:6619976/1‑140 (MQ=255)

                      cACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCt                                                                                                                  <  1:3961188/140‑1 (MQ=255)

                        cTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGt                                                                                                                <  2:8076822/140‑1 (MQ=255)

                         tGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTa                                                                                                               <  2:2083452/140‑1 (MQ=255)

                                   cGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGc                                                                                                     >  1:4202195/1‑140 (MQ=255)

                                     tATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCg                                                                                                   <  2:8243450/140‑1 (MQ=255)

                                           tGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTa                                                                                                                       <  2:1011241/114‑1 (MQ=255)

                                           tGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTa                                                                                                                       >  1:1011241/1‑114 (MQ=255)

                                            gggCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGGTGCTGt                                                                                            <  2:3315099/140‑1 (MQ=255)

                                             ggCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGtt                                                                                           >  2:7768879/1‑140 (MQ=255)

                                             ggCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGtt                                                                                           >  1:7075297/1‑140 (MQ=255)

                                             ggCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGtt                                                                                           >  2:1417539/1‑140 (MQ=255)

                                             ggCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGtt                                                                                           >  1:9640776/1‑140 (MQ=255)

                                               cAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTgg                                                                                         <  2:5533324/140‑1 (MQ=255)

                                                  tGCAGTGGCGTCGTGGGTTGTCGGGCATCTGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACCAAAACTTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATCGGCCGTTGCTGTTGGTCa                                                                                      >  2:5092320/1‑140 (MQ=255)

                                                  tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACCGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCa                                                                                      >  2:3164972/1‑140 (MQ=255)

                                                  tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCa                                                                                      >  1:1275403/1‑140 (MQ=255)

                                                  tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCa                                                                                      >  1:5393714/1‑140 (MQ=255)

                                                  tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGAGTGTGCGCCAAAGCGAGAGGAACTACTCCTCGCACACAAACACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCACTAGGCCGATGCTGTTGGTCa                                                                                      >  2:7262323/1‑140 (MQ=255)

                                                  tGCAGTGGCGTCGGGGTTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCACGCACAAAAAAACGTATAGATCGCTGCGACAGTGGCTAGTGCTGTACTGAATAGGCCGTTGCTGTTGGTCa                                                                                      >  2:7375949/1‑140 (MQ=255)

                                                   gCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCaa                                                                                     <  1:5619802/140‑1 (MQ=255)

                                                    cAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAg                                                                                    <  1:2145771/140‑1 (MQ=255)

                                                    cAGCGGCCTCGTGGGTTGTCGGGCATCGGGGCGGTGCTTGTGCGCCACCGCGCGAGGACCTACACCACGCACCCCAACCCGTATAGAGCGCTGCGACACTGGCGCCTGCTGTACTCAATAGGCCGGGGCTGTTGGTCAAg                                                                                    <  2:3392241/140‑1 (MQ=255)

                                                       tGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAATCGTATAGATCGCTGCGACAGTGGCTCATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAgcgc                                                                                 >  2:4018711/1‑140 (MQ=255)

                                                        ggCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGcc                                                                                <  2:1271912/140‑1 (MQ=255)

                                                        ggCGTCGTGGGTTGGCGGGCATCGGGGCGGTGATTGAGCGCCAACGCGCGAGGACCTACACCTCGAACACAAAAACCTATAGAACGCTGCCACAGTGGCGAATGCTGTACACAATAGGACGATGCTGAAGGACAAGCGcc                                                                                >  2:8340594/1‑140 (MQ=255)

                                                         gCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCa                                                                               <  1:7450452/140‑1 (MQ=255)

                                                         gCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCa                                                                               <  1:482080/140‑1 (MQ=255)

                                                         gCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCa                                                                               >  1:8786705/1‑140 (MQ=255)

                                                         gCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCa                                                                               <  1:8416748/140‑1 (MQ=255)

                                                           gtcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCAct                                                                             <  2:1610824/140‑1 (MQ=255)

                                                           gtcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCAct                                                                             <  1:5045609/140‑1 (MQ=255)

                                                            tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActt                                                                            >  2:4687883/1‑140 (MQ=255)

                                                            tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActt                                                                            <  1:1960475/140‑1 (MQ=255)

                                                            tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActt                                                                            <  1:1573417/140‑1 (MQ=255)

                                                               tGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgct                                                                         <  1:379173/140‑1 (MQ=255)

                                                               tGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgct                                                                         >  1:2278475/1‑140 (MQ=255)

                                                                 ggTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgcttg                                                                       <  2:801412/140‑1 (MQ=255)

                                                                 ggTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgcttg                                                                       <  1:7283695/140‑1 (MQ=255)

                                                                 ggTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgcttg                                                                       <  1:9506628/140‑1 (MQ=255)

                                                                    tGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATc                                                                    >  1:4289389/1‑140 (MQ=255)

                                                                         ggCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAg                                                               <  1:8990677/140‑1 (MQ=255)

                                                                            aTCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAACCGTATCGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCAc                                                            <  2:8486470/140‑1 (MQ=255)

                                                                             tCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCCCACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCAcc                                                           >  2:6536565/1‑140 (MQ=255)

                                                                             tCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCAcc                                                           >  1:205385/1‑140 (MQ=255)

                                                                                gggCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGt                                                        >  1:223090/1‑140 (MQ=255)

                                                                                    ggTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTAttt                                                    <  1:3229329/140‑1 (MQ=255)

                                                                                     gTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTAtttt                                                   >  1:3684238/1‑140 (MQ=255)

                                                                                      tGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTg                                                  <  1:6393493/140‑1 (MQ=255)

                                                                                      tGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTg                                                  <  1:6753903/140‑1 (MQ=255)

                                                                                      tGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTg                                                  >  2:5480449/1‑140 (MQ=255)

                                                                                           gtgCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAAGAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATc                                             >  2:9653359/1‑140 (MQ=255)

                                                                                            tgCGCCATCGCGCGAGGAACTACTCCTCGCACACAAAATCGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCt                                            <  2:2898804/140‑1 (MQ=255)

                                                                                            tgCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCt                                            <  1:344422/140‑1 (MQ=255)

                                                                                             gcgcCAACGCGCGAGGAACTACTCCTCGCACACACACACGTCTCGATCGCTGCGACAGTGGCTAAAGCTGTACTCACTAGGCCGTTGCTGATGGGCAAGCGCCACTTGCTTGATCACCCGCACCGGTATTTTGCTATCTa                                           <  2:6810917/140‑1 (MQ=255)

                                                                                              cgcCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTaa                                          >  1:8645936/1‑140 (MQ=255)

                                                                                                    cgcgcgAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAat                                    >  1:5918147/1‑140 (MQ=255)

                                                                                                      cgcgAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAatat                                  >  1:4491034/1‑140 (MQ=255)

                                                                                                      cgcgAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAatat                                  <  1:1414263/140‑1 (MQ=255)

                                                                                                      cgcgAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAatat                                  <  1:7964202/140‑1 (MQ=255)

                                                                                                      cgcgAGGAACGACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAatat                                  <  1:3169557/140‑1 (MQ=255)

                                                                                                           tgAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGAc                             <  1:8184327/139‑1 (MQ=255)

                                                                                                                 aCTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAAc                       >  2:3575306/1‑140 (MQ=255)

                                                                                                                 aCTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAAc                       >  1:6971716/1‑140 (MQ=255)

                                                                                                                 aCTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAAc                       >  1:7757560/1‑140 (MQ=255)

                                                                                                                  ctcctcGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACt                      <  2:461925/140‑1 (MQ=255)

                                                                                                                  ctcctcGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACt                      <  2:8899067/140‑1 (MQ=255)

                                                                                                                     ctcGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcgg                   >  1:9090290/1‑140 (MQ=255)

                                                                                                                       cGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCt                                                                                                                  >  2:9329687/1‑43 (MQ=255)

                                                                                                                       cGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggca                 >  2:5984898/1‑140 (MQ=255)

                                                                                                                       cGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggca                 >  1:5664372/1‑140 (MQ=255)

                                                                                                                               ataaCTTATAGATCGCTGCGACAGTGGCTAATGCTTTACTCAATAGGCCGTTGCTGTTGGTCAAGCGACACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggcatccggcat         <  1:8093735/138‑1 (MQ=255)

                                                                                                                                 aaCGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCggcatccggcatcc       <  2:5308518/140‑1 (MQ=255)

                                                                                                                                  aCGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGgcatccggcatccg      <  2:4230432/140‑1 (MQ=255)

                                                                                                                                      ataGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGgcg  >  2:7869903/1‑140 (MQ=255)

                                                                                                                                      ataGATCGCAGCGACAGTGGCTAATGCTGTACTCAATAGGCCGATGCTGTTGGTCAAGCGCAACTTGATTGATCACCAGCACCGGTATTTTGGTCTCGATCTGAATATGTGTCTGAAACTCGGCCTCCGGCTTCCGGgcg  >  2:7516313/1‑140 (MQ=255)

GCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCG  >  NZ_CP025268/2185961‑2186234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`