BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,224,824	A→G	C401R (TGT→CGT)	bglX ←	beta‑glucosidase BglX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,224,824	0	A	G	99.8%	2084.9 / NA	601	C401R (TGT→CGT)	bglX	beta‑glucosidase BglX
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (245/354); minor base T (0/1); total (246/355)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 601 total aligned reads displayed.

ATCCCGGTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATA  >  NZ_CP025268/2224688‑2224960

    cGGTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTc                                                                                                                                   >  2:7850906/1‑140 (MQ=255)

     ggTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCTCGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGAAACGTTTCGAGACGGTTTTTCa                                                                                                                                  >  2:4757536/1‑140 (MQ=255)

     ggTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCa                                                                                                                                  >  2:206877/1‑140 (MQ=255)

      gTCAGTACGGTCCCGGATTGATCGGCAACACAGGCTGCGGACCAGCTGCCCATCAGGTCTCGTTTACTGTCCGCCTGTGGCCCTGCCAGCGCTATGTGGGCCGAGTGTTGCAGAGGTTACGTTTCGAGGCGGTTGTTcgg                                                                                                                                 >  2:7403783/1‑138 (MQ=255)

      gTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAg                                                                                                                                 <  2:1246570/140‑1 (MQ=255)

      gTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAg                                                                                                                                 >  1:9052833/1‑140 (MQ=255)

      gTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACGGTCCGCCAGTGGCCCCACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAg                                                                                                                                 <  2:799751/140‑1 (MQ=255)

       tCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGc                                                                                                                                <  1:1011675/140‑1 (MQ=255)

               gTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCaa                                                                                                                        >  1:3163183/1‑140 (MQ=255)

                tCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAg                                                                                                                       >  1:9529472/1‑140 (MQ=255)

                  aCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCt                                                                                                                     >  1:4170239/1‑140 (MQ=255)

                     gATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTc                                                                                                                  >  1:7549779/1‑140 (MQ=255)

                      aTTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcg                                                                                                                 <  1:1752864/140‑1 (MQ=255)

                          aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgcg                                                                                                             >  1:7865100/1‑140 (MQ=255)

                          aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgcg                                                                                                             >  1:8909919/1‑140 (MQ=255)

                          aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgcg                                                                                                             >  2:4893005/1‑140 (MQ=255)

                          aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgcg                                                                                                             <  1:700444/140‑1 (MQ=255)

                          aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgcg                                                                                                             >  2:904944/1‑140 (MQ=255)

                            cGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGcc                                                                                                           <  1:1432556/140‑1 (MQ=255)

                               cAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCACGCTTTCGCGCGCCACt                                                                                                        <  2:8091531/140‑1 (MQ=255)

                                aaCACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACtt                                                                                                       >  1:5026227/1‑140 (MQ=255)

                                 acacCGGCTGCGGACCAGCCGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTc                                                                                                      >  1:1907329/1‑140 (MQ=255)

                                    cctgCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcg                                                                                                   <  2:6572711/137‑1 (MQ=255)

                                    ccGGCTTCGGTCCAGCTGCCCATCACGTCACGATTACTGTCCGCCAGTTGCCCAACCACCGCAATGGAGGCCGATATGTGCAGCGGTAACGTTTCTAGACCGTTTTTCAGCAACACCCTGCTTTCGCGCGCCACATCAcg                                                                                                   >  2:8387345/1‑140 (MQ=255)

                                    ccGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcg                                                                                                   <  1:7206598/140‑1 (MQ=255)

                                    ccGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcg                                                                                                   <  1:8430855/140‑1 (MQ=255)

                                    ccGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcg                                                                                                   <  1:3890187/140‑1 (MQ=255)

                                    ccGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcg                                                                                                   <  2:1800266/140‑1 (MQ=255)

                                    ccGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcg                                                                                                   <  2:4170239/140‑1 (MQ=255)

                                    ccGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcg                                                                                                   <  2:4600991/140‑1 (MQ=255)

                                    ccGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcg                                                                                                   <  2:4987204/140‑1 (MQ=255)

                                    ccGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcg                                                                                                   <  2:5952119/140‑1 (MQ=255)

                                    ccGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTGTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCGTTCGCGCGCCACTTCAcg                                                                                                   >  2:4442139/1‑140 (MQ=255)

                                     cGGCTGCGGACCAGCTGCCCATCACGTCTCGTGTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGTCGGTTTTTCAGCAACACCAAGCGTTCGCGCGCCACTTCAcgc                                                                                                  >  2:3084947/1‑140 (MQ=255)

                                      ggCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcgcg                                                                                                 <  1:8762558/140‑1 (MQ=255)

                                               ccAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTAc                                                                                        <  1:3246859/140‑1 (MQ=255)

                                                  gCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGt                                                                                     >  1:2893308/1‑140 (MQ=255)

                                                   cTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTg                                                                                    <  1:1148103/140‑1 (MQ=255)

                                                       ccATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTGTACGGTGCAgg                                                                                >  1:9785634/1‑140 (MQ=255)

                                                          tcacgtcacgTTTACTGTCCGCCAGTGGCCCAACCACAGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCTCTCGCCACTTCACGCGATTCTTTACGGTTCAggcgg                                                                             >  2:6908326/1‑140 (MQ=255)

                                                              gtcacgtTGACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCACGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCttt                                                                         <  2:3228915/140‑1 (MQ=255)

                                                                cacgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCg                                                                       >  1:7760047/1‑140 (MQ=255)

                                                                cacgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCg                                                                       >  1:3030618/1‑140 (MQ=255)

                                                                cacgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCg                                                                       <  1:4359190/140‑1 (MQ=255)

                                                                 acgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCgg                                                                      <  2:7248086/140‑1 (MQ=255)

                                                                   gtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCa                                                                    <  1:7989503/140‑1 (MQ=255)

                                                                   gtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCa                                                                    >  1:653036/1‑140 (MQ=255)

                                                                   gtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCa                                                                    <  2:5142782/140‑1 (MQ=255)

                                                                   gtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCa                                                                    <  1:4854325/140‑1 (MQ=255)

                                                                   gtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCAGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCGAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCa                                                                    <  2:9597027/140‑1 (MQ=255)

                                                                    tttACGGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCAt                                                                   <  1:2305424/140‑1 (MQ=255)

                                                                     ttACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCAtt                                                                  >  1:5607348/1‑140 (MQ=255)

                                                                     ttACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCAtt                                                                  >  1:3328353/1‑140 (MQ=255)

                                                                     ttACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCAtt                                                                  >  2:8629370/1‑140 (MQ=255)

                                                                       aCTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTACACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTgg                                                                <  2:7128955/140‑1 (MQ=255)

                                                                           tCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATc                                                            >  1:3592877/1‑140 (MQ=255)

                                                                             cGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCa                                                          >  2:3504358/1‑140 (MQ=255)

                                                                             cGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCa                                                          <  1:3990164/140‑1 (MQ=255)

                                                                             cGCCAGTGGACCAACCACCGCTATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCa                                                          <  2:773031/140‑1 (MQ=255)

                                                                               ccAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGATTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCAcc                                                        >  1:5244566/1‑140 (MQ=255)

                                                                                     gCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCa                                                  >  1:3382954/1‑140 (MQ=255)

                                                                                       ccAACCACCGCAATGGTGGCCGATTTTTTCTGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCTAGCCACTTCACGCGATTCTTTACGGTGCAGGCTGCTTTCGGCATTGGTATCCACCGGGTCAGa                                                <  2:5385911/140‑1 (MQ=255)

                                                                                             accGCAATGGTTGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCttt                                          <  1:5500073/140‑1 (MQ=255)

                                                                                               cGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCATACTCTTTCg                                        >  2:2904944/1‑140 (MQ=255)

                                                                                               cGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCg                                        >  1:932184/1‑140 (MQ=255)

                                                                                                gCAATGGTGGCCGATTTTTTCAGCGTTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCTCGCGATTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCgg                                       <  1:2092586/140‑1 (MQ=255)

                                                                                                  cagggtggCCGTTTTTTTCAGCGGTACCGTTTCGAGTCGGTTTTTCAGCACCACCTAGCTTTCGCGCGCCCCGTCACGCGCTTCTTGACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGcc                                     <  2:6752852/137‑1 (MQ=255)

                                                                                                  aaTGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGcc                                     <  1:5903593/140‑1 (MQ=255)

                                                                                                   aTGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGccc                                    >  2:3957054/1‑140 (MQ=255)

                                                                                                     ggtggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCa                                  <  1:3703389/140‑1 (MQ=255)

                                                                                                     ggtggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCa                                  <  2:3653910/140‑1 (MQ=255)

                                                                                                     ggtggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCa                                  <  2:1624475/140‑1 (MQ=255)

                                                                                                      gtggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCaa                                 <  1:6811187/140‑1 (MQ=255)

                                                                                                       tggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCaaa                                <  2:2374980/140‑1 (MQ=255)

                                                                                                        ggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAAt                               <  2:4290539/140‑1 (MQ=255)

                                                                                                        ggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAAt                               <  2:3774625/140‑1 (MQ=255)

                                                                                                        ggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAAt                               <  1:241391/140‑1 (MQ=255)

                                                                                                         gCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCg                                                                       >  2:6324920/1‑99 (MQ=255)

                                                                                                         gCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCg                                                                       <  1:6324920/99‑1 (MQ=255)

                                                                                                         gCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATg                              >  2:5565297/1‑140 (MQ=255)

                                                                                                         gCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATg                              >  2:2649483/1‑140 (MQ=255)

                                                                                                         gCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATg                              >  1:9443720/1‑140 (MQ=255)

                                                                                                         gCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATg                              >  1:7414315/1‑140 (MQ=255)

                                                                                                         gCCGATTTTGTCAGCGGGAACGTTTCGAGACGGTTTTTCTGCAACACCCAGCTTTCGCGCGCCACGTCACGCGCTTCTTTCCGGTGCAGGGGGCTTTCGGCATTGGTCTACACCGGGTCAGACTCTTTCGGccccccagg                              >  2:8849116/1‑135 (MQ=255)

                                                                                                          ccGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATgg                             <  2:5695448/140‑1 (MQ=255)

                                                                                                               tttttCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTa                        <  1:1258238/140‑1 (MQ=255)

                                                                                                                ttttCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTAt                       <  1:2513379/140‑1 (MQ=255)

                                                                                                                    cAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGt                   <  1:4504098/140‑1 (MQ=255)

                                                                                                                      gCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCg                 <  2:9488841/140‑1 (MQ=255)

                                                                                                                       cGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGt                <  1:2649483/140‑1 (MQ=255)

                                                                                                                       cGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGt                <  2:7541485/140‑1 (MQ=255)

                                                                                                                         gTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTa              <  1:8624108/140‑1 (MQ=255)

                                                                                                                           aaCGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCTGGCGGCTTTCGGCATTGGAATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTaaa            <  2:6087467/140‑1 (MQ=255)

                                                                                                                           aaCGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTaaa            <  1:4705250/140‑1 (MQ=255)

                                                                                                                           aaCGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTaaa            <  2:82615/140‑1 (MQ=255)

                                                                                                                            aCGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAaac           <  2:1005857/140‑1 (MQ=255)

                                                                                                                              gTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAaacaa         <  1:8268304/140‑1 (MQ=255)

                                                                                                                                 tccagaCGGTTTTTCAGCAACACCAAGATTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATAGGTATCAACCGGGTCAGACTCTGACGGCCCCAAAAGGCTGTATGGGTCGTTAAACAAccc      >  2:5887191/4‑140 (MQ=255)

                                                                                                                                  cGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACTCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGTCTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAAcccc     <  2:2181142/140‑1 (MQ=255)

                                                                                                                                  cGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAAcccc     <  2:9174561/140‑1 (MQ=255)

ATCCCGGTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATA  >  NZ_CP025268/2224688‑2224960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`