BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,280,334	A→G	342 (TAA→TAG)	yejE →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,280,334	0	A	G	99.4%	1628.9 / NA	471	342 (TAA→TAG)	yejE	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (244/224); minor base T (3/0); total (247/224)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 471 total aligned reads displayed.

CTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGT  >  NZ_CP025268/2280197‑2280470

 tgTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCAt                                                                                                                                       <  1:1008870/140‑1 (MQ=255)

   ttACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTAGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGa                                                                                                                                     <  1:8742398/140‑1 (MQ=255)

       aaGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCa                                                                                                                                 <  1:857946/140‑1 (MQ=255)

       aaGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCa                                                                                                                                 <  2:1849929/140‑1 (MQ=255)

       aaGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCa                                                                                                                                 <  1:4033416/140‑1 (MQ=255)

        aGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGCGTAGCATGACGCaa                                                                                                                                >  2:2447293/1‑140 (MQ=255)

          ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATNTTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAAc                                                                                                                              >  1:6305664/1‑140 (MQ=255)

          ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGCTCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAAc                                                                                                                              >  2:4220207/1‑140 (MQ=255)

          ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAAc                                                                                                                              >  1:9019495/1‑140 (MQ=255)

                  aaCCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAg                                                                                                                      <  1:95067/140‑1 (MQ=255)

                    ccTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATAGTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCg                                                                                                                    >  1:6715097/1‑140 (MQ=255)

                      ttCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTTTCTTTGCTGATCTTAATTGGTGATGCCGTCCGCGACGCATTTGATCATAGTAAGGCGGTGTTGCATGACGAATACTGTGTTTGCgct                                                                                                                  >  2:7568466/1‑138 (MQ=255)

                         aGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGa                                                                                                               >  1:7793260/1‑140 (MQ=255)

                          ggCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTATTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCTTGACGCAATCTCTGTTAGCGATTGaa                                                                                                              >  2:8302019/1‑140 (MQ=255)

                           gCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGaaa                                                                                                             >  1:559156/1‑140 (MQ=255)

                                gTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTg                                                                                                        >  1:3170117/1‑140 (MQ=255)

                                gTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGAGTGAAAATTTg                                                                                                        >  1:5067051/1‑140 (MQ=255)

                                     ttGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGt                                                                                                   >  1:2933574/1‑140 (MQ=255)

                                     ttGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGt                                                                                                   <  2:1257139/140‑1 (MQ=255)

                                         gATCACCGCCTTCTTGTCGGTGGCGAGATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGACAATTTGTCGGTGGGt                                                                                               >  2:5372493/1‑140 (MQ=255)

                                             aCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTc                                                                                           >  1:5894500/1‑140 (MQ=255)

                                              ccGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCg                                                                                          >  1:2145516/1‑140 (MQ=255)

                                               cGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGc                                                                                         <  2:1413286/140‑1 (MQ=255)

                                               cGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGc                                                                                         <  1:2493816/140‑1 (MQ=255)

                                               cGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGc                                                                                         <  1:1252889/140‑1 (MQ=255)

                                                      ttGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGc                                                                                  <  1:8463105/140‑1 (MQ=255)

                                                      ttGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGc                                                                                  <  2:6957591/140‑1 (MQ=255)

                                                        gTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                                <  2:501514/140‑1 (MQ=255)

                                                        gTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                                <  1:7057599/140‑1 (MQ=255)

                                                          cGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAc                                                                              <  2:4389124/140‑1 (MQ=255)

                                                          cGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAc                                                                              <  2:8841348/140‑1 (MQ=255)

                                                           ggTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAcc                                                                             <  2:7698861/140‑1 (MQ=255)

                                                           ggTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAcc                                                                             >  1:970909/1‑140 (MQ=255)

                                                           ggTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAcc                                                                             <  2:1580382/140‑1 (MQ=255)

                                                             tGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgt                                                                           <  1:5387792/140‑1 (MQ=255)

                                                             tGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgt                                                                           <  2:428235/140‑1 (MQ=255)

                                                             tGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgt                                                                           <  2:1652087/140‑1 (MQ=255)

                                                             tGGCGATATTAGTGTCTTTGCTGATCTTTATGGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgt                                                                           <  1:7526560/140‑1 (MQ=255)

                                                              ggCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGc                                                                                         <  2:1141673/125‑1 (MQ=255)

                                                              ggCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgta                                                                          >  2:3665738/1‑140 (MQ=255)

                                                                cGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacg                                                                        >  2:7429916/1‑140 (MQ=255)

                                                                cGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacg                                                                        >  1:2630512/1‑140 (MQ=255)

                                                                cGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacg                                                                        <  1:1414831/140‑1 (MQ=255)

                                                                cGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacg                                                                        <  2:3422252/140‑1 (MQ=255)

                                                                  atatTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacgta                                                                      <  2:9160113/140‑1 (MQ=255)

                                                                  atatTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGGGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacgta                                                                      <  2:9414685/140‑1 (MQ=255)

                                                                    attatgGTCTTTGCTGATCTTTATTGGACCAGCCGACCGCGACGCATTTGATCCTACTAAGGCGGTGTAGCATGACGCAAACTCGGTTAGCGATTGAATATTTGTCGGTGGGTTTTCGCCATCCGCAAACCGTACTTACa                                                                    <  2:8994004/140‑1 (MQ=255)

                                                                       attGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTGCGTACagta                                                                 <  1:8088459/140‑1 (MQ=255)

                                                                          ttCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTc                                                              <  2:8597276/139‑1 (MQ=255)

                                                                          gTCTTTGCTGATCTTTATTGGGGAAGCCGTCCGCGACGCATTTGATCCTAATAATGCGGTGTAGCATGACGCAAACTCTGTTAGAGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTc                                                              >  2:4457713/1‑140 (MQ=255)

                                                                            cTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGTCGCAAACTCTGTTAGCGATTGATAATTTGTCGGTGGGTTTTCGCCATCAGCATACCGTACGTACAGTAGTCaa                                                            <  2:3114340/140‑1 (MQ=255)

                                                                            cTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCaa                                                            <  2:8445450/140‑1 (MQ=255)

                                                                             tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAat                                                           >  2:9541466/1‑140 (MQ=255)

                                                                             tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAat                                                           >  1:7228574/1‑140 (MQ=255)

                                                                             tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAat                                                           >  2:3921451/1‑140 (MQ=255)

                                                                             tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGGAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAat                                                           >  1:7213007/1‑140 (MQ=255)

                                                                               tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                         <  1:3248379/140‑1 (MQ=255)

                                                                               tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                         >  2:4575897/1‑140 (MQ=255)

                                                                               tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATATGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAATATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                         >  2:673044/1‑140 (MQ=255)

                                                                                 cTGATCTTTATTTCTGAAGCCGTCCGCGACGCATTGGCTCCTAATCAGGCGGGGTAGCATGACGCAAACTCTGGTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatgctg                                                       <  2:7740376/140‑1 (MQ=255)

                                                                                 cTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatgatg                                                       <  2:4105927/140‑1 (MQ=255)

                                                                                   gATCTGTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGtt                                                     >  1:2303615/1‑140 (MQ=255)

                                                                                     tCTTTATTGGTGAAGCCGTCCGCGACTCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTTTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTAAGTACAGTAGTCAATGATGTTTc                                                   <  2:2945845/140‑1 (MQ=255)

                                                                                     tCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGtt                                                     <  1:3711562/138‑1 (MQ=255)

                                                                                        ttACTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTCGCATGACGCAAACGCAGTTAGCGATAGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGAGGTTTCACt                                                <  2:5174510/140‑1 (MQ=255)

                                                                                         tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCTATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTa                                               <  1:6477563/140‑1 (MQ=255)

                                                                                         tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTa                                               >  1:7588559/1‑140 (MQ=255)

                                                                                         tATTGGTGAAGCCGTCCGCGACGCATTAGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTGCACTa                                               <  2:5842868/140‑1 (MQ=255)

                                                                                                   gCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTCGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAgg                                     >  2:9717075/1‑140 (MQ=255)

                                                                                                    ccGTCCGCCACGCATTTGATCCTAAGAAGGCGGTGTAGCATGACGCAAACTCTGATAGCTATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGc                                    <  2:7801256/140‑1 (MQ=255)

                                                                                                     cGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCt                                   >  2:597224/1‑140 (MQ=255)

                                                                                                          gcgACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGa                              <  1:4085953/140‑1 (MQ=255)

                                                                                                             aCGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAc                           <  2:2743762/140‑1 (MQ=255)

                                                                                                              cGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcg                          >  1:8348787/1‑140 (MQ=255)

                                                                                                              cGCATTTGATACTAATAAGGCGGTTTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcg                          <  2:7081175/140‑1 (MQ=255)

                                                                                                               gCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgc                         <  2:6098923/140‑1 (MQ=255)

                                                                                                                 aTTTGAGCGTAAGAATGCGTTGTAGCATGACGCAAACTCTGTTAGAGCTTGAACATTTGGCGGTTGGTTTTCGCCATCAGCATCCCGTACGTACAGTTGTCAATGCTGTTTCTCGACAGATTGAGGCTGGCGAAAcgcgg                       <  2:6435142/140‑3 (MQ=255)

                                                                                                                   ttGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgctggc                     >  1:4820150/1‑140 (MQ=255)

                                                                                                                     gATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgctggcgc                   <  1:1046162/140‑1 (MQ=255)

                                                                                                                        ccTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgctggcgctgg                >  1:9107411/1‑140 (MQ=255)

                                                                                                                          taataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCtggtg              >  1:2775006/1‑140 (MQ=255)

                                                                                                                           aataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCtggtgg             <  1:5979264/140‑1 (MQ=255)

                                                                                                                           aataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCtggtgg             >  1:3336817/1‑140 (MQ=255)

                                                                                                                            ataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtggg            <  1:5467667/140‑1 (MQ=255)

                                                                                                                            ataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtggg            <  2:2305771/140‑1 (MQ=255)

                                                                                                                             taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtgggt           <  1:1596321/140‑1 (MQ=255)

                                                                                                                             taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtgggt           <  1:1741994/140‑1 (MQ=255)

                                                                                                                             taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtgggt           <  2:8033918/140‑1 (MQ=255)

                                                                                                                             taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtgggt           <  2:5558536/140‑1 (MQ=255)

                                                                                                                                ggCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGTGATTTCGCCATCTGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACATATTGAGGCTGGCGAAACTCTGGCGATGGTGGGTGAg        >  2:7222379/1‑140 (MQ=255)

                                                                                                                                ggCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAg        >  1:4300987/1‑140 (MQ=255)

                                                                                                                                    gtgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAg    >  1:5712031/1‑140 (MQ=255)

                                                                                                                                    gtgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAg    >  2:1061495/1‑140 (MQ=255)

                                                                                                                                     tgtATCATGACGCAAACTCTGTTAGAGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg   >  2:2361199/1‑140 (MQ=255)

                                                                                                                                     tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg   >  2:5469698/1‑140 (MQ=255)

                                                                                                                                     tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg   >  1:1858321/1‑140 (MQ=255)

                                                                                                                                     tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg   >  2:5734253/1‑140 (MQ=255)

                                                                                                                                     tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg   >  1:5264392/1‑140 (MQ=255)

                                                                                                                                     tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg   >  2:9587255/1‑140 (MQ=255)

                                                                                                                                      gtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGt  >  1:6845993/1‑140 (MQ=255)

CTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGT  >  NZ_CP025268/2280197‑2280470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`