BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,415,086	G→C	T101S (ACC→AGC)	yfbS ←	SLC13 family permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,415,086	0	G	C	99.8%	2179.9 / NA	628	T101S (ACC→AGC)	yfbS	SLC13 family permease
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (272/355); minor base A (1/0); total (273/355)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 628 total aligned reads displayed.

TAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTT  >  NZ_CP025268/2414949‑2415222

       cccGGCAAAACTTAACGGCATCATCAGACGTGCCGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATca                                                                                                                                 >  2:2829151/1‑140 (MQ=255)

       cccGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATca                                                                                                                                 >  1:668417/1‑140 (MQ=255)

       cccGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATca                                                                                                                                 >  1:1701134/1‑140 (MQ=255)

       cccGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATca                                                                                                                                 >  1:5381019/1‑140 (MQ=255)

       cccGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATca                                                                                                                                 >  1:395018/1‑140 (MQ=255)

        cctgCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGTTAGCGACAAAGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATcaa                                                                                                                                >  2:7911735/4‑140 (MQ=255)

                 cTTAACGGCATCATCAGACGAGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCTCGGGAATAAAGATAGCGACAACGCCGGGTGAACTCTTAATCGCGCCAAGCCCCGCGACGCTCAGCATCAACAATACCaa                                                                                                                       >  1:5911825/1‑140 (MQ=255)

                     aCGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACAtt                                                                                                                   <  1:1032408/140‑1 (MQ=255)

                     aCGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACAtt                                                                                                                   <  1:5624264/140‑1 (MQ=255)

                         catcatCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGa                                                                                                               <  1:95303/140‑1 (MQ=255)

                         catcatCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGa                                                                                                               <  2:1965819/140‑1 (MQ=255)

                         catcatCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGa                                                                                                               >  1:5055479/1‑140 (MQ=255)

                          atcatcAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGAt                                                                                                              >  2:6212193/1‑140 (MQ=255)

                           tcatcaGACGTGACGGCGACGGGTGCATACGCATGGCAACGCTTAACACGACGGGAATGAAGTTAGCGTCTGCGCCGGTTGAACTCATAACGGAGCCAAGCCCCGCGACGCTCAGACTCAACTAGCCCCTCATTTTgttg                                                                                                             >  2:7132139/1‑137 (MQ=255)

                             atcaGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGAGAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTc                                                                                                           <  2:655624/140‑1 (MQ=255)

                              tcaGACGTGACGGCGACGTGGGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACCACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCa                                                                                                          <  2:1233383/140‑1 (MQ=255)

                                   cGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTAtt                                                                                                     >  1:9681791/1‑140 (MQ=255)

                                   cGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTAtt                                                                                                     >  1:7132829/1‑140 (MQ=255)

                                   cGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTAtt                                                                                                     >  1:4329625/1‑140 (MQ=255)

                                       aCGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGccc                                                                                                 >  2:9013467/1‑140 (MQ=255)

                                       aCGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGccc                                                                                                 >  2:6369099/1‑140 (MQ=255)

                                       aCGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGccc                                                                                                 >  1:2141824/1‑140 (MQ=255)

                                        cGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCg                                                                                                <  1:5125856/140‑1 (MQ=255)

                                           cGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCaa                                                                                             <  1:9121467/140‑1 (MQ=255)

                                                tttGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACTCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGACCGCAACTTTg                                                                                        >  2:9467847/1‑140 (MQ=255)

                                                 ttGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACACCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGa                                                                                       <  2:4704677/140‑1 (MQ=255)

                                                  tGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGAc                                                                                      <  2:1772381/140‑1 (MQ=255)

                                                  tGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGAc                                                                                      <  2:746089/140‑1 (MQ=255)

                                                  tGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGAc                                                                                      <  2:2572426/140‑1 (MQ=255)

                                                       aCGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGcc                                                                                 <  1:6916245/140‑1 (MQ=255)

                                                       aCGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGcc                                                                                 <  1:3581103/140‑1 (MQ=255)

                                                        cGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCa                                                                                    <  2:1390122/136‑1 (MQ=255)

                                                          cATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATg                                                                              >  1:8362964/1‑140 (MQ=255)

                                                              gCAACGCTTAACACCACGGGAATAAAGAAAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATAAACAAAACCCACATTTTGAGTTCACTAATCCCCTCAACTTTGACCCGcccagtacc                                                                          >  2:497383/1‑133 (MQ=255)

                                                               cAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTccc                                                                         >  1:1326781/1‑140 (MQ=255)

                                                                aaCGCTTCACACCCCGGGAATAAACATACCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCa                                                                        <  2:6672645/140‑1 (MQ=255)

                                                                aaCGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACCTTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCa                                                                        <  1:8054640/140‑1 (MQ=255)

                                                                  cGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCAtt                                                                      <  2:2098211/140‑1 (MQ=255)

                                                                  cGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCAtt                                                                      >  2:5703868/1‑140 (MQ=255)

                                                                  cGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACAGTTTGATTTCACTATTGCCCGCAACTGTGACCAGCCATGTTCCCAtt                                                                      >  2:9059526/1‑140 (MQ=255)

                                                                    cTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTAc                                                                    <  2:8369443/140‑1 (MQ=255)

                                                                         caccacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTgg                                                               >  2:3152038/1‑140 (MQ=255)

                                                                          cccacGGGAATAAAGAGAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAACCCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  1:3140988/139‑1 (MQ=255)

                                                                          accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATCAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  1:7586053/140‑1 (MQ=255)

                                                                          accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  2:9539042/140‑1 (MQ=255)

                                                                          accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  2:1620358/140‑1 (MQ=255)

                                                                            cacGGGAATAAAGATCGCGACAACGCCGGTTGAACTCAGAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATGTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCaa                                                            >  2:9304107/1‑140 (MQ=255)

                                                                             acGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAac                                                           >  1:1207166/1‑140 (MQ=255)

                                                                             acGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAac                                                           >  2:839938/1‑140 (MQ=255)

                                                                             acGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAac                                                           >  1:2868907/1‑140 (MQ=255)

                                                                              cGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAaca                                                          <  1:2446224/140‑1 (MQ=255)

                                                                               gggAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAacac                                                         <  1:6412218/140‑1 (MQ=255)

                                                                               gggAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAacac                                                         <  2:4211405/140‑1 (MQ=255)

                                                                                   aTAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGt                                                     >  2:6151708/1‑140 (MQ=255)

                                                                                    tAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                    <  2:8608039/140‑1 (MQ=255)

                                                                                    tAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                    <  2:7724104/140‑1 (MQ=255)

                                                                                          tAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGAcc                                              <  1:8994876/140‑1 (MQ=255)

                                                                                           aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACt                                                                                           <  1:9856873/94‑1 (MQ=255)

                                                                                           aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTCCGGACCa                                             >  1:7948611/1‑140 (MQ=255)

                                                                                           aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCa                                             >  1:8160712/1‑140 (MQ=255)

                                                                                           aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCa                                             >  1:7759863/1‑140 (MQ=255)

                                                                                           aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCa                                             >  2:4299807/1‑140 (MQ=255)

                                                                                            gCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCaa                                            >  1:6526671/1‑140 (MQ=255)

                                                                                                  aCGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACTGTGGCAACACCGGTACGGACCAAACCATc                                      >  1:62448/1‑140 (MQ=255)

                                                                                                  aCGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATc                                      <  1:9217617/140‑1 (MQ=255)

                                                                                                   cGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCg                                     >  1:3390719/1‑140 (MQ=255)

                                                                                                    gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  1:4234875/140‑1 (MQ=255)

                                                                                                    gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  2:800121/140‑1 (MQ=255)

                                                                                                    gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  1:6789533/140‑1 (MQ=255)

                                                                                                    gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  2:8244246/140‑1 (MQ=255)

                                                                                                    gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  2:305566/140‑1 (MQ=255)

                                                                                                        gTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCaat                                >  2:628091/1‑140 (MQ=255)

                                                                                                         ttGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCACCaata                               <  2:7638277/140‑1 (MQ=255)

                                                                                                          tGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCaataa                              >  2:5815088/1‑140 (MQ=255)

                                                                                                                   cgcggcgcCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    >  2:5538398/5‑125 (MQ=255)

                                                                                                                   aaaCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCGACTGTGACCAGCCAGGTTCCCATGACGGTGGCAACACCGGTACGGACCACTCCATCGCCAATACTAGCCAAgg                     >  2:9649350/1‑140 (MQ=255)

                                                                                                                   aaaCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCAt                                                                               >  1:4029237/1‑82 (MQ=255)

                                                                                                                   aaaCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCAt                                                                               <  2:4028474/82‑1 (MQ=255)

                                                                                                                   aaaCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAgg                     >  2:3377881/1‑140 (MQ=255)

                                                                                                                     aCGCGCCAAGCCCCGCGACGCTCAGCATCAACAACACCACCATTATGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAggcg                   <  1:3750341/140‑1 (MQ=255)

                                                                                                                     aCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAggcg                   <  1:1945504/140‑1 (MQ=255)

                                                                                                                     aCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAggcg                   <  1:2710895/140‑1 (MQ=255)

                                                                                                                         gcCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATCATAAACAAGGCGGCac               >  1:7294326/1‑139 (MQ=255)

                                                                                                                          ccAAGCCCCGCGACGCTCAGCATCAACACAACCAACAGTTTGATTTCACTCTTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGGGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAAt              <  2:4494077/140‑1 (MQ=255)

                                                                                                                             aGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAg           <  1:7374839/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCTTCAACAAAACCAACTTTTTGATTTCTCTCTTGCCCGCAACTTTGACCTGGCATGTTCCCATGACGGTGGCAACTCCGGTACGGACCAAACCCTCGCCAATAAGAAACAAGGCGGCAATCAGGa         <  2:8058960/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAACCCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCTTGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:7675508/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:4634933/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:8672806/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  2:9138360/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:3848912/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  2:3618614/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:4854337/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCACATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  2:5184891/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCAACAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  2:2402876/140‑1 (MQ=255)

                                                                                                                                cccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGAc        <  2:5063797/140‑1 (MQ=255)

                                                                                                                                cccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGAc        <  2:2189651/140‑1 (MQ=255)

                                                                                                                                cccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGAc        <  1:148459/140‑1 (MQ=255)

                                                                                                                                 ccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACa       <  2:6995825/140‑1 (MQ=255)

                                                                                                                                   gcgACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAAc     >  2:379208/1‑140 (MQ=255)

TAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTT  >  NZ_CP025268/2414949‑2415222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`