BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,453,263	T→C	274 (TAA→TAG)	yfcO ←	fimbrial adhesin YfcO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,453,263	0	T	C	99.8%	1805.4 / NA	516	274 (TAA→TAG)	yfcO	fimbrial adhesin YfcO
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (200/315); minor base A (1/0); total (201/315)
Fisher's exact test for biased strand distribution p-value = 3.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 516 total aligned reads displayed.

TGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCG  >  NZ_CP025268/2453126‑2453399

    ggTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAAc                                                                                                                                    <  1:3815358/140‑1 (MQ=255)

      tGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTc                                                                                                                                  >  1:5756535/1‑140 (MQ=255)

      tGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTc                                                                                                                                  >  2:4296732/1‑140 (MQ=255)

        aTGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCAt                                                                                                                                <  1:1288219/140‑1 (MQ=255)

        aTGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCAt                                                                                                                                <  2:6326695/140‑1 (MQ=255)

         tGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGTGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCAtt                                                                                                                               <  1:1634701/140‑1 (MQ=255)

         tGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCAtt                                                                                                                               <  1:1035473/140‑1 (MQ=255)

         tGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCAtt                                                                                                                               <  1:2911172/140‑1 (MQ=255)

          gcggcgTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTc                                                                                                                              <  1:2023952/140‑1 (MQ=255)

             gcgTTGTTGGTGTTGATTGCAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGTGCTACAACTCATTCAGt                                                                                                                           <  2:6766608/140‑1 (MQ=255)

             gcgTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGt                                                                                                                           <  1:7258653/140‑1 (MQ=255)

             gcgTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGt                                                                                                                           <  2:75533/140‑1 (MQ=255)

             gcgTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGt                                                                                                                           <  1:4465419/140‑1 (MQ=255)

              cgTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTa                                                                                                                          <  1:6530276/140‑1 (MQ=255)

              cgTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTa                                                                                                                          <  1:9119511/140‑1 (MQ=255)

              cgTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTa                                                                                                                          <  2:5323623/140‑1 (MQ=255)

                ttgttgGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTCTg                                                                                                                        >  2:1535784/1‑140 (MQ=255)

                ttgttgGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATg                                                                                                                        >  2:8480626/1‑140 (MQ=255)

                  gttgGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTa                                                                                                                      >  2:2351427/1‑140 (MQ=255)

                        gtTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCAtt                                                                                                                               <  2:2079349/125‑1 (MQ=255)

                           gattgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTa                                                                                                                          <  2:5969305/127‑1 (MQ=255)

                           gattgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTa                                                                                                                          >  1:5969305/1‑127 (MQ=255)

                           gattgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCg                                                                                                             <  1:4911265/140‑1 (MQ=255)

                           gattgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCg                                                                                                             >  2:6207222/1‑140 (MQ=255)

                            attgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCgg                                                                                                            >  1:2217762/1‑140 (MQ=255)

                             ttgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGc                                                                                                           <  1:6826971/140‑1 (MQ=255)

                                 aGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGt                                                                                                       >  1:2660612/1‑140 (MQ=255)

                                 aGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGt                                                                                                       >  1:7388902/1‑140 (MQ=255)

                                 aGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGt                                                                                                       <  1:4324951/140‑1 (MQ=255)

                                      aaGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAAt                                                                                                  >  2:7394056/1‑140 (MQ=255)

                                          gTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGTGTAATGGCAGGGGCGCATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGGACTGGAACCGGCAAGTAGCATCCAt                                                                                              <  2:1757973/140‑1 (MQ=255)

                                          gTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGAt                                                                                              <  1:8681053/140‑1 (MQ=255)

                                                   gccgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCAt                                                                                     >  1:6753649/1‑140 (MQ=255)

                                                    ccgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATa                                                                                    <  2:9613501/140‑1 (MQ=255)

                                                     cgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTCATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTCAGGGCTACAACACATTCAGTATGTACTGGAACCGGCACGTAGAATCGATTGGCTGCATAc                                                                                   >  2:8664969/1‑140 (MQ=255)

                                                     cgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTTTCTCAGGGCGACTTCGCATTCAGTATGGACTGGAAGCGGCGGGTAGACTCGCTTGGCTGGATAc                                                                                   >  2:8170194/1‑140 (MQ=255)

                                                       gAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATActg                                                                                 <  1:789761/140‑1 (MQ=255)

                                                        aGTTGCCCTGCGGCGTTGAGCGCGTTGCGCCAGGTCCGGGGAAGGGCAGGGGCGACTGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTTGATGCATActgc                                                                                <  2:3784816/140‑1 (MQ=255)

                                                            gCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGt                                                                            >  2:1121638/1‑140 (MQ=255)

                                                            gCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGt                                                                            <  1:2728632/140‑1 (MQ=255)

                                                                 gaggagTTGAGCGAGTTGCGCCAGGTACGGGTAATGGCAGGGCCGAATGCCCCGGCCTGGTGTATCTAAGGGCTCCATCTCATTCAGAATGTACGGGACCCGGCAAGTAGACTCGATGGGCTGCAGACTGCTGGTATCat                                                                       <  2:2991561/140‑2 (MQ=255)

                                                                  aggagTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAac                                                                      >  2:2164122/1‑140 (MQ=255)

                                                                   ggagTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAaca                                                                     <  1:8856783/140‑1 (MQ=255)

                                                                     cgTTGAGCGCGTTGCGCCAGGTACGGGTAAGGGCAGGGGCGAAGGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGTATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                                   <  2:1930700/139‑1 (MQ=255)

                                                                     agTTGAGCGCGTTTCGACAGTTACGGGTAATTGCAGGGTCTAATTCCCCTGCCTGTTGTATCTAAGGGCTACAACTCATTCATTATGTACTGGAAAGGTAAAGTAGAATCGTTTGTCTGCATACTGCTGGTATCAAcacg                                                                   <  2:9071311/140‑1 (MQ=255)

                                                                     agTTGAGCGCGTTGCGCCCGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGACACGGAAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                                   <  2:7273518/140‑1 (MQ=255)

                                                                     agTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGACTGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                                   <  2:1331638/140‑1 (MQ=255)

                                                                     agTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                                   <  2:6453766/140‑1 (MQ=255)

                                                                     agTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                                   <  1:5342777/140‑1 (MQ=255)

                                                                     agTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                                   <  1:5259739/140‑1 (MQ=255)

                                                                       ttGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgca                                                                 <  1:2454732/140‑1 (MQ=255)

                                                                       ttGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgca                                                                 <  1:9313558/140‑1 (MQ=255)

                                                                       ttGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgca                                                                 <  1:9743440/140‑1 (MQ=255)

                                                                       ttGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                                   >  1:9405311/1‑138 (MQ=255)

                                                                          aGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGa                                                              <  1:6642764/140‑1 (MQ=255)

                                                                            cgcgTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGAcc                                                            >  1:7797792/1‑140 (MQ=255)

                                                                            cgcgTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGAcc                                                            >  2:3155883/1‑140 (MQ=255)

                                                                             gcgTTGCGCCAGGTGCGGGTAATGGCAGGGGCGACTGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCa                                                           <  2:7943973/140‑1 (MQ=255)

                                                                             gcgTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCa                                                           <  2:8918271/140‑1 (MQ=255)

                                                                             gcgTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCa                                                           <  1:6096840/140‑1 (MQ=255)

                                                                             gcgTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCa                                                           <  1:3568452/140‑1 (MQ=255)

                                                                               gTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCata                                                         >  1:845994/1‑140 (MQ=255)

                                                                               gTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCata                                                         <  2:9282514/140‑1 (MQ=255)

                                                                                    gcCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCgtg                                                    >  1:4587992/1‑140 (MQ=255)

                                                                                      cAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTa                                                  <  1:4110508/140‑1 (MQ=255)

                                                                                        ggTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACAAGCACGACCATATCTAGTAAc                                                >  2:6882853/1‑140 (MQ=255)

                                                                                              ggTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTg                                          <  1:5898261/140‑1 (MQ=255)

                                                                                               gTACTGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                         <  1:6981185/140‑1 (MQ=255)

                                                                                               gTACTGGCAGGGGCGAATGCCCCTGCCTGGCGTCTCTAAGGGCTACACCACATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                         <  2:9438021/140‑1 (MQ=255)

                                                                                               gTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGCCCATATCGTGTAACTGTTTGt                                         <  2:3631969/140‑1 (MQ=255)

                                                                                               gTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                         <  1:7516352/140‑1 (MQ=255)

                                                                                               gTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                         <  2:8814498/140‑1 (MQ=255)

                                                                                               gTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                         <  2:2830741/140‑1 (MQ=255)

                                                                                                tAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTc                                        <  1:1782288/140‑1 (MQ=255)

                                                                                                  aTGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGNACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCg                                      >  1:6301812/1‑140 (MQ=255)

                                                                                                    ggCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGtt                                    >  1:3583735/1‑140 (MQ=255)

                                                                                                    ggCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGtt                                    >  1:8067313/1‑140 (MQ=255)

                                                                                                    ggCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGtt                                    >  2:246015/1‑140 (MQ=255)

                                                                                                      gaGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTTGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTtat                                  <  1:8251605/139‑1 (MQ=255)

                                                                                                      cAGGGGCGAATGCCCCTGCCTGGTTTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACTCACGACCATATCGTGTAACTGTTTGTCCGTtat                                  <  2:698435/140‑1 (MQ=255)

                                                                                                      cAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTtat                                  <  1:2346455/140‑1 (MQ=255)

                                                                                                         gggCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAaca                                                                     >  2:4823575/1‑102 (MQ=255)

                                                                                                         gggCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAaca                                                                     <  1:4823575/102‑1 (MQ=255)

                                                                                                         gggCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAgg                               >  1:2822265/1‑140 (MQ=255)

                                                                                                         gggCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAgg                               >  2:3436307/1‑140 (MQ=255)

                                                                                                         gggCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAgg                               >  2:4655490/1‑140 (MQ=255)

                                                                                                            cGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGgtg                            <  2:7209850/140‑1 (MQ=255)

                                                                                                                      tgcctgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGc                  <  1:1393979/140‑1 (MQ=255)

                                                                                                                       gcctgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCg                 >  1:1181043/1‑140 (MQ=255)

                                                                                                                             tgtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCg           >  2:6375458/1‑140 (MQ=255)

                                                                                                                             tgtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCg           >  2:5822095/1‑140 (MQ=255)

                                                                                                                             tgtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGGTATAGGGTGTCGATTTGGCGTGACCg           >  1:3251885/1‑140 (MQ=255)

                                                                                                                              gtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCga          >  1:3113035/1‑140 (MQ=255)

                                                                                                                                aTCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGAtt                      >  1:6425884/1‑126 (MQ=255)

                                                                                                                                 tCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCCTCTCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGAc       >  2:4518601/1‑140 (MQ=255)

                                                                                                                                 tCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGAc       >  1:893124/1‑140 (MQ=255)

                                                                                                                                 tCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATAACGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGAc       >  1:5498884/1‑140 (MQ=255)

                                                                                                                                  cTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACt      >  2:5786942/1‑140 (MQ=255)

                                                                                                                                     aGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTcc   >  2:5050866/1‑140 (MQ=255)

                                                                                                                                      gggATACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGATGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTACGTTATAGGGTGTCGTTTTGGCTTGACCGAGACTTACg  >  2:8277728/1‑140 (MQ=255)

TGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCG  >  NZ_CP025268/2453126‑2453399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`