BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	247,291	A→G	250 (TAA→TAG)	yafL →	C40 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	247,291	0	A	G	99.8%	1677.8 / NA	490	250 (TAA→TAG)	yafL	C40 family peptidase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (222/267); minor base C (0/1); total (222/268)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 490 total aligned reads displayed.

GTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑G‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAA  >  NZ_CP025268/247154‑247425

gtgtATTGGGGCGATGGGCAATTTCTCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                                                        <  1:6817416/140‑1 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  2:1548705/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  1:7285564/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  1:9275112/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  1:9426938/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       <  1:3695034/140‑1 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  2:1969740/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  1:2379199/1‑140 (MQ=255)

  gtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑g                                                                                                                                     >  2:5077112/1‑140 (MQ=255)

   tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑gg                                                                                                                                    >  2:4514856/1‑139 (MQ=255)

   tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑gg                                                                                                                                    >  1:7545184/1‑139 (MQ=255)

     tttGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑ggat                                                                                                                                  <  2:6385512/140‑4 (MQ=255)

     tttGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑ggat                                                                                                                                  <  1:7662093/140‑4 (MQ=255)

     tttGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCACTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑ggat                                                                                                                                  <  2:9705244/140‑4 (MQ=255)

        gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAg                                                                                                                               >  1:6993815/1‑140 (MQ=255)

        gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAg                                                                                                                               >  1:5404362/1‑140 (MQ=255)

        gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAg                                                                                                                               <  2:7879651/140‑1 (MQ=255)

        gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAg                                                                                                                               >  1:854178/1‑140 (MQ=255)

        gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAg                                                                                                                               >  1:4227216/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                          >  2:6191785/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                          >  1:4152154/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                          >  2:7625981/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                          >  2:7229230/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTGTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                          >  2:8161452/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGCAAAAGCCGATTAGCCGACCCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGAGTTTGTAGGACGG‑ATCAGGCGtg                                                                                                                          >  2:1112303/1‑139 (MQ=255)

                ggCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTAc                                                                                                                       >  1:9009118/1‑140 (MQ=255)

                      ttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCa                                                                                                                 <  2:1633383/140‑1 (MQ=255)

                        aTCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGCTTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATc                                                                                                               <  2:2839483/140‑1 (MQ=255)

                          cGAGTCGCCACGTACCGGCGAAACCATTCGGATCAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCg                                                                                                             >  2:9139506/1‑140 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATACGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  1:8087167/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  2:1026926/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  1:7184613/140‑1 (MQ=255)

                              tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                         <  1:5244342/140‑1 (MQ=255)

                               cGCCACGTACCGGCGAACCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTGTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGGAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGt                                                                                                        >  2:8455214/1‑140 (MQ=39)

                               cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCCGGACCATTTTTTGGGCGCGCTCAGGATTTGGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGt                                                                                                        >  2:2938640/1‑140 (MQ=38)

                               cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGt                                                                                                        >  1:9619473/1‑140 (MQ=255)

                                gCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTGTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGtt                                                                                                       <  2:7477295/140‑1 (MQ=255)

                                  cACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGt                                                                                                     <  1:6433798/140‑1 (MQ=255)

                                   aCGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTa                                                                                                    <  1:4690719/140‑1 (MQ=255)

                                       aCCTGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                                <  2:581771/140‑1 (MQ=255)

                                       aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                                <  1:1365856/140‑1 (MQ=255)

                                       aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                                >  2:4359810/1‑140 (MQ=255)

                                        ccGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAg                                                                                               <  2:4860189/140‑1 (MQ=255)

                                         cGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAgg                                                                                              >  1:6169335/1‑140 (MQ=255)

                                            cGACACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTGTACGCCGCATCCGGCAGTTGTACGCAGGTGc                                                                                           >  2:6957860/1‑140 (MQ=39)

                                              aaaCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCt                                                                                         >  1:2274362/1‑140 (MQ=255)

                                               aaCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTg                                                                                        >  2:4173661/1‑140 (MQ=255)

                                                  cATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                                                     <  1:8715160/140‑1 (MQ=255)

                                                  cATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                                                     <  1:2437554/140‑1 (MQ=255)

                                                  cATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                                                     <  1:5701405/140‑1 (MQ=255)

                                                     tCGGATAAGCCGATTAGCCGAACCTTTCTGGCCGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGa                                                                                  >  1:9762685/1‑140 (MQ=255)

                                                     tCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGa                                                                                  >  1:7893472/1‑140 (MQ=255)

                                                        gATAAGCCGATTAGCCGAACCTTTCGGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGc                                                                               <  1:4623115/140‑1 (MQ=255)

                                                         aTAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCt                                                                              >  1:1148346/1‑140 (MQ=255)

                                                           aaGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTgg                                                                            <  1:4509260/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:218325/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:1990539/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:1861974/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:2674568/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  2:8827824/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           >  1:6573818/1‑140 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           >  2:2716049/1‑140 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:3556547/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:512930/140‑1 (MQ=255)

                                                            aGCCGAATAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  2:9528186/140‑1 (MQ=255)

                                                             gCCGATTAGCCGAACCTTTCTGGCAGGACCATGTTGTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgcg                                                                          >  2:5647542/1‑140 (MQ=37)

                                                              ccGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgcgc                                                                         <  1:5054951/140‑1 (MQ=255)

                                                              ccGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgcgc                                                                         >  2:4696610/1‑140 (MQ=255)

                                                                   tAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCtt                                                                    >  1:16/1‑140 (MQ=255)

                                                                    aGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTa                                                                   <  1:4378665/140‑1 (MQ=255)

                                                                      ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                 <  2:9426938/140‑1 (MQ=255)

                                                                      ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                 <  2:9041041/140‑1 (MQ=255)

                                                                      ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                 <  2:4017055/140‑1 (MQ=255)

                                                                      ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                 <  2:2252454/140‑1 (MQ=255)

                                                                        gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                               >  2:1384461/1‑140 (MQ=255)

                                                                         aaCCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATg                                                              >  2:1910395/1‑140 (MQ=255)

                                                                              ttCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTTACGGAAGAGACGATTTTGTAGGACGG‑ATAATGCGTTTACGCCGCATCCGTAAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTAc                                                         >  1:3017715/1‑140 (MQ=25)

                                                                              ttCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTAc                                                         >  2:3842770/1‑140 (MQ=255)

                                                                                cTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGa                                                       <  2:4754356/140‑1 (MQ=255)

                                                                                cTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGa                                                       >  2:2592897/1‑140 (MQ=255)

                                                                                cTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGa                                                       <  2:3691385/140‑1 (MQ=255)

                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                                 <  1:2935562/140‑1 (MQ=255)

                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                                 >  1:8400030/1‑140 (MQ=255)

                                                                                       gACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgcg                                                >  2:336652/1‑140 (MQ=255)

                                                                                        aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGGTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGAAGCGACGCTGGCGCGTCTAATCATGCCTACGAGCCCGCGa                                               >  2:5470307/1‑140 (MQ=38)

                                                                                                 tGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGAAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCg                                      >  2:7116030/1‑140 (MQ=255)

                                                                                                       cgcgcAGGATTTTGACGGAATAGACGATTTTGTAGGAC‑GTATAAGTCGTTTACGCCGCATCCGGCAGTTGTAACCAGGAGCCTTATGCGACGCTGGCTCGACATATAAATCCTACGAGCCAGCGAAAAAATGCGAGCCGc                                >  2:6608757/1‑140 (MQ=19)

                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                                <  2:392584/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                           <  2:3171515/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                           <  2:2491891/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                           <  1:5450476/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                           <  1:1463400/140‑1 (MQ=255)

                                                                                                                 tttGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGata                      <  1:486825/140‑1 (MQ=255)

                                                                                                                        gAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca               >  2:3562506/1‑140 (MQ=255)

                                                                                                                           gagaCGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAAcc            <  1:6928532/140‑1 (MQ=255)

                                                                                                                            agaCGATTGTGTAGGACGG‑ATCAGGCGATTACGCCGCAGCCGGCACTTGTACGCAGGTGCCTGATGCGACGCTGGCGAGTCTTATCATGCCTAGGCGCCCGCGACTATTTGCGAGCCGCTTTCCCGATATAACACAACCt           <  2:7790019/140‑1 (MQ=21)

                                                                                                                                 aTTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTg      <  1:3337739/140‑1 (MQ=255)

                                                                                                                                   tttGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATAGAAAACAACCTCAGTGcc    <  1:1816508/140‑1 (MQ=25)

                                                                                                                                     tGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                 >  1:1097971/1‑77 (MQ=14)

                                                                                                                                     tGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                 >  1:388698/1‑77 (MQ=14)

GTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑G‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAA  >  NZ_CP025268/247154‑247425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`