BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,529,956	T→C	309 (TAA→TAG)	yfeR ←	LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,529,956	0	T	C	100.0%	1619.4 / NA	466	309 (TAA→TAG)	yfeR	LysR family transcriptional regulator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (268/198); total (268/198)

Only 100 of 466 total aligned reads displayed.

GCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCA  >  NZ_CP025268/2529819‑2530091

cacattactacggggattcagtatcgctctgcggatgacaaactgggcgaagagttttaccaggaggcgataatctactccatcaaagttaacttctgattgattactgccggggctcccggcagtctgacttCACTCta                                                                                                                                       >  2:7654996‑M2/134‑140 (MQ=255)

cacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCta                                                                                                                                       >  1:4280683‑M2/134‑140 (MQ=255)

 acattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCtat                                                                                                                                      <  1:4710737‑M2/8‑1 (MQ=255)

 acattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCtat                                                                                                                                      <  2:9301506‑M2/8‑1 (MQ=255)

  cattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCtata                                                                                                                                     <  1:7263226‑M2/9‑1 (MQ=255)

    ttactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATc                                                                                                                                   <  1:1032385‑M2/11‑1 (MQ=255)

    ttactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATc                                                                                                                                   <  2:5184471‑M2/11‑1 (MQ=255)

     tactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCt                                                                                                                                  >  2:6801115‑M2/129‑140 (MQ=255)

     tactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCt                                                                                                                                  >  1:9439866‑M2/129‑140 (MQ=255)

     tactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCt                                                                                                                                  >  1:1553496‑M2/129‑140 (MQ=255)

     tactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgagtgattactgccggggctcccggcagtatgaattCACTCTATATCt                                                                                                                                  >  1:8709297‑M2/129‑140 (MQ=255)

         acggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATa                                                                                                                              <  1:1341214‑M2/16‑1 (MQ=255)

          cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                             >  1:2216437‑M2/124‑140 (MQ=255)

            gggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACaa                                                                                                                           <  2:6524477‑M2/19‑1 (MQ=255)

             ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAAc                                                                                                                          >  2:5647760‑M2/121‑140 (MQ=255)

             ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAAc                                                                                                                          >  1:412108‑M2/121‑140 (MQ=255)

              gattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACg                                                                                                                         <  1:8460068‑M2/21‑1 (MQ=255)

              gattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACg                                                                                                                         <  2:1214735‑M2/21‑1 (MQ=255)

               attccgtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                        >  2:8163656‑M2/119‑140 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcatatttaacgtctgattgattactgccggggctcccggcagtttgaattCACGCTATAAATGATACAACgg                                                                                                                        >  1:8000582‑M2/119‑140 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                        >  2:8616870‑M2/119‑140 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                        >  1:8332153‑M2/119‑140 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                        >  1:4489142‑M2/119‑140 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                        >  1:2784544‑M2/119‑140 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                        >  1:3141388‑M2/119‑140 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgagtgattactgccggggctcccggcagtatgaattCCCTCTATATCTGATACAACgg                                                                                                                        >  1:5810835‑M2/119‑140 (MQ=255)

                 tcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGAt                                                                                                                      >  2:4208783‑M2/117‑140 (MQ=255)

                 tcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGAt                                                                                                                      >  2:3414834‑M2/117‑140 (MQ=255)

                 tcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGAt                                                                                                                      >  1:2018791‑M2/117‑140 (MQ=255)

                  cagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATc                                                                                                                     <  1:2956949‑M2/25‑1 (MQ=255)

                     tatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATcccc                                                                                                                  >  1:3686063‑M2/113‑140 (MQ=255)

                        cgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTc                                                                                                               <  1:6221716‑M2/31‑1 (MQ=255)

                          atatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTccc                                                                                                             >  2:3752966‑M2/108‑140 (MQ=255)

                           tatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgacttCACTCTATCTCTGATACAACGGATCCCCTTCCCg                                                                                                            >  2:9102223‑M2/107‑140 (MQ=255)

                             tgctgatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                          <  1:5622598‑M2/36‑1 (MQ=255)

                             tgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                          >  1:5002735‑M2/105‑140 (MQ=255)

                               cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                        <  1:8105540‑M2/38‑1 (MQ=255)

                               cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                        >  1:5744311‑M2/103‑140 (MQ=255)

                                 gatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGc                                                                                                      <  1:8235119‑M2/40‑1 (MQ=255)

                                     acaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtt                                                                                                  >  1:7094673‑M2/97‑140 (MQ=255)

                                         actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcg                                                                                              >  2:5770616‑M2/93‑140 (MQ=255)

                                         actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcg                                                                                              >  1:5987666‑M2/93‑140 (MQ=255)

                                         actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcg                                                                                              >  2:8815266‑M2/93‑140 (MQ=255)

                                           tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                            <  1:894508‑M2/50‑1 (MQ=255)

                                           tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                            <  2:4335613‑M2/50‑1 (MQ=255)

                                           tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggcccccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                            <  1:7363695‑M2/50‑1 (MQ=255)

                                            gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                           >  1:6814005‑M2/90‑140 (MQ=255)

                                            gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                           >  2:6859942‑M2/90‑140 (MQ=255)

                                            gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccgacagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                           >  1:2455458‑M2/90‑140 (MQ=255)

                                             ggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAtt                                                                                          >  1:9279554‑M2/89‑140 (MQ=255)

                                              gcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtctgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTg                                                                                         >  2:1488215‑M2/88‑140 (MQ=255)

                                                 aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATCTCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                      <  1:5112446‑M2/56‑1 (MQ=255)

                                                 aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                      <  2:167636‑M2/56‑1 (MQ=255)

                                                 aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                      <  2:9382617‑M2/56‑1 (MQ=255)

                                                 aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                      <  1:384432‑M2/56‑1 (MQ=255)

                                                  agatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCt                                                                                     <  1:1793776‑M2/57‑1 (MQ=255)

                                                   gatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                                    <  2:8460305‑M2/58‑1 (MQ=255)

                                                             aggatgcgataatcgactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGAGCCCCGTCCCGCCCAGCCATTAGCGCAGTGCCCTGGTCTCGTAcc                                                                          <  2:316926‑M2/68‑1 (MQ=255)

                                                               gatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGGACCAc                                                                        >  1:964038‑M2/71‑140 (MQ=255)

                                                                atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAATGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                       <  1:6072021‑M2/71‑1 (MQ=255)

                                                                atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                       <  1:9108522‑M2/71‑1 (MQ=255)

                                                                   cgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTcc                                                                    >  1:6647311‑M2/67‑140 (MQ=255)

                                                                           tactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCtata                                                                                                                                     <  2:3642862‑M2/9‑1 (MQ=255)

                                                                           tactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCGTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGc                                                            <  2:1943156‑M2/82‑1 (MQ=255)

                                                                             ctccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt                                                          >  2:261454‑M2/57‑140 (MQ=255)

                                                                             ctccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt                                                          >  2:7833369‑M2/57‑140 (MQ=255)

                                                                              tccatcaaatttaacttctgattgattactgccggggctcccggcagtctgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTc                                                         >  2:231665‑M2/56‑140 (MQ=255)

                                                                              tccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAGTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTc                                                         >  1:6978939‑M2/56‑140 (MQ=255)

                                                                                catcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                                       >  1:330547‑M2/54‑140 (MQ=255)

                                                                                  tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                                     >  2:300169‑M2/52‑140 (MQ=255)

                                                                                  tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                                     >  2:4572083‑M2/52‑140 (MQ=255)

                                                                                  tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                                     <  1:907601‑M2/89‑1 (MQ=255)

                                                                                   caaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcgg                                                    >  1:2290476‑M2/51‑140 (MQ=255)

                                                                                    aaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcggc                                                   <  1:2631748‑M2/91‑1 (MQ=255)

                                                                                          aacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                      >  2:4716860‑M2/44‑99 (MQ=255)

                                                                                                tgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                       >  2:9664231‑M2/38‑140 (MQ=255)

                                                                                                tgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                       >  1:6445673‑M2/38‑140 (MQ=255)

                                                                                                tgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                       >  1:7816046‑M2/38‑140 (MQ=255)

                                                                                                tgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                       <  2:2148849‑M2/103‑1 (MQ=255)

                                                                                                  attgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAAc                                     <  2:248815‑M2/105‑1 (MQ=255)

                                                                                                            gccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTgg                                              >  1:1191303‑M2/26‑121 (MQ=255)

                                                                                                            gccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCt                           >  2:3566433‑M2/26‑140 (MQ=255)

                                                                                                            gccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCt                           >  2:6120587‑M2/26‑140 (MQ=255)

                                                                                                              cggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgc                         <  1:5461328‑M2/117‑1 (MQ=255)

                                                                                                                gggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc                       >  2:9560060‑M2/22‑140 (MQ=255)

                                                                                                                gggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc                       >  1:357790‑M2/22‑140 (MQ=255)

                                                                                                                 ggctcccggcagtatgaattCACTCTATCTCGGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGGACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa                      <  2:3927819‑M2/120‑1 (MQ=255)

                                                                                                                 ggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa                      <  2:2789568‑M2/120‑1 (MQ=255)

                                                                                                                  gctcccggcagtatgaattCACTCTCTATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAc                     <  1:6689282‑M2/121‑1 (MQ=255)

                                                                                                                     cccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGACAGTGAACGGTTCTTCCTGCGCACCAg                  >  2:7098228‑M2/17‑140 (MQ=255)

                                                                                                                     cccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAg                  >  1:6500045‑M2/17‑140 (MQ=255)

                                                                                                                     cccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAg                  >  1:2322148‑M2/17‑140 (MQ=255)

                                                                                                                      ccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGc                 <  2:7418689‑M2/125‑1 (MQ=255)

                                                                                                                            gtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAAc           <  1:3718902‑M2/131‑1 (MQ=255)

                                                                                                                            gtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGGCTCGTACCACGTCCCATCAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAAc           >  2:4878828‑M2/10‑140 (MQ=255)

                                                                                                                             tatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGACAGTGAACGGTTCTTCCTGCGCACCAGCATCAACt          >  2:6386648‑M2/9‑140 (MQ=255)

                                                                                                                               tgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTgg        >  1:754720‑M2/7‑140 (MQ=255)

                                                                                                                                gaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGc       >  1:5394743‑M2/6‑140 (MQ=255)

                                                                                                                                gaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGc       <  1:990612‑M2/135‑1 (MQ=255)

                                                                                                                                 aattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCg      <  1:8053969‑M2/136‑1 (MQ=255)

GCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCA  >  NZ_CP025268/2529819‑2530091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`