BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,622,099	T→C	234 (TAA→TAG)	hda ←	DnaA inactivator Hda

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,622,099	0	T	C	99.8%	1981.2 / NA	569	234 (TAA→TAG)	hda	DnaA inactivator Hda
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (312/256); minor base A (0/1); total (312/257)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 569 total aligned reads displayed.

GATGTGCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCCA  >  NZ_CP025268/2621962‑2622235

    tgCTCCATCGCTAGTTGAAGCACATTTCCGGAAGCGAAGCATGCCACGTCTTATCAGGACTACGAATACTAAGGATTAAAATCGCGTAGTTCGTAAAAGGCGTTCGCGCCGCAACCGACAAAAAACACCTTATCTACAAc                                                                                                                                    >  2:3770189/1‑140 (MQ=255)

     gCTCCATCGCTCGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACt                                                                                                                                   >  2:4502324/1‑140 (MQ=255)

     gCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACt                                                                                                                                   >  2:2420623/1‑140 (MQ=255)

     gCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACt                                                                                                                                   >  1:3832188/1‑140 (MQ=255)

      cTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACtt                                                                                                                                  >  1:6607686/1‑140 (MQ=255)

          aTCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGa                                                                                                                              <  1:8525170/140‑1 (MQ=255)

           tCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGaa                                                                                                                             >  2:1066123/1‑140 (MQ=255)

            cGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAAt                                                                                                                            >  2:4148408/1‑140 (MQ=255)

                aGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGACTACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAAtttct                                                                                                                        >  1:4486017/1‑140 (MQ=255)

                  ttGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACGTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAAtttcttt                                                                                                                      <  2:1948210/140‑1 (MQ=255)

                       gCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACaa                                                                                                                 >  1:5914334/1‑140 (MQ=255)

                             tGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGaa                                                                                                           >  1:6742336/1‑140 (MQ=255)

                              gCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCTCGTAGTTCGGATAAGGCTTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAAt                                                                                                          <  2:1473418/140‑1 (MQ=255)

                              gCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAAt                                                                                                          <  2:3201755/140‑1 (MQ=255)

                               ccGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATg                                                                                                         <  1:2303828/140‑1 (MQ=255)

                                ctgATGCGACGCTTTCCACGTCTTATCCTGCCTACGAATAATATTGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  2:7939900/138‑1 (MQ=39)

                                cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGCTTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACCCCTTATCTACAACATCAGAATTACTTTCACACACGGAATgg                                                                                                        <  2:4040459/140‑1 (MQ=39)

                                cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  1:2031924/140‑1 (MQ=255)

                                cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  1:1376297/140‑1 (MQ=255)

                                cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  1:4608809/140‑1 (MQ=255)

                                cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAAGACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  1:2793000/140‑1 (MQ=255)

                                    tGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAg                                                                                                    >  2:728063/1‑140 (MQ=255)

                                        aCGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTa                                                                                                <  1:5606360/140‑1 (MQ=255)

                                           cTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGt                                                                                             <  2:7033859/140‑1 (MQ=255)

                                                 aCGTCTAATCCGGCCTACGAATACGATGGATTACCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACCATACCCACCTGAGCTACACCTACAGAAGTTCTTTCACAAACGGAATGGTCAGCTTACGTTGcgcg                                                                                       <  2:1299339/140‑1 (MQ=255)

                                                   gTCTTATCCGGCCTACGAATACTCTGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACCACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGt                                                                                     >  2:721414/1‑140 (MQ=255)

                                                      ttATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAAt                                                                                  <  1:1018146/140‑1 (MQ=255)

                                                        aTCCGGCCTACGAATACTTTGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCg                                                                                <  2:663907/140‑1 (MQ=255)

                                                        aTCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCg                                                                                >  2:9495214/1‑140 (MQ=255)

                                                         tCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGa                                                                               >  2:8953336/1‑140 (MQ=255)

                                                           cGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAACCACCTTATCTACAACGTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACg                                                                             <  1:5322276/140‑1 (MQ=255)

                                                           cGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACg                                                                             <  2:2908517/140‑1 (MQ=255)

                                                             gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           >  2:60361/1‑140 (MQ=255)

                                                             gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  1:4011478/140‑1 (MQ=255)

                                                             gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  1:1264609/140‑1 (MQ=255)

                                                             gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  2:5061757/140‑1 (MQ=255)

                                                             gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  2:2655964/140‑1 (MQ=255)

                                                              ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCAc                                                                          >  2:4715797/1‑140 (MQ=255)

                                                              ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCAc                                                                          >  1:9668229/1‑140 (MQ=255)

                                                              ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCAc                                                                          >  1:9008157/1‑140 (MQ=255)

                                                                tACGAATACTCTGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATCAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGa                                                                        <  2:8246850/140‑1 (MQ=255)

                                                                tACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGACTGTCTTTCACAAACGGACTGGTCAACTTAGGTGGCGCGGTACTCGACGCCCGa                                                                        >  1:7179212/1‑140 (MQ=38)

                                                                tACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGa                                                                        >  1:7299887/1‑140 (MQ=255)

                                                                  cGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATc                                                                      >  2:9581082/1‑140 (MQ=255)

                                                                        cTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTg                                                                >  1:8375676/1‑140 (MQ=255)

                                                                        cTAGGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTg                                                                >  2:4315913/1‑140 (MQ=255)

                                                                          aTGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGAt                                                              >  2:3063305/1‑140 (MQ=255)

                                                                           tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATc                                                             >  1:5104355/1‑140 (MQ=255)

                                                                           tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATc                                                             >  2:2227939/1‑140 (MQ=255)

                                                                            ggATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATcc                                                            >  2:8497113/1‑140 (MQ=255)

                                                                            ggATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATcc                                                            >  1:5680152/1‑140 (MQ=255)

                                                                             gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  1:9528187/140‑1 (MQ=255)

                                                                             gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  1:7453770/140‑1 (MQ=255)

                                                                             gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  1:4906512/140‑1 (MQ=255)

                                                                             gATTAACTTCGCGTAGTTCGGATAAGGCGTGCGCGCCGCATCCGACAATAAACACCTTATCTACACCTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  2:8662586/140‑1 (MQ=255)

                                                                              aTTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCaa                                                          >  1:3325459/1‑140 (MQ=255)

                                                                              aTTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCaa                                                          >  1:4759760/1‑140 (MQ=255)

                                                                               ttAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                         <  1:298095/140‑1 (MQ=255)

                                                                               ttAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                         <  2:3480144/140‑1 (MQ=255)

                                                                               ttAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCAGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                         <  2:8804534/140‑1 (MQ=255)

                                                                                 aaCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGt                                                       <  2:4658435/140‑1 (MQ=255)

                                                                                 aaCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGt                                                       <  2:6431415/140‑1 (MQ=255)

                                                                                 aaCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGt                                                       <  1:9794665/140‑1 (MQ=255)

                                                                                  aCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACATTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTCCGTTGCGCGGTAATCGACGCACGATCCAACTGATCCATCGTc                                                      >  2:2048822/1‑140 (MQ=255)

                                                                                   cTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCa                                                     >  1:171669/1‑140 (MQ=255)

                                                                                   cTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCa                                                     >  1:1615930/1‑140 (MQ=255)

                                                                                   cTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCa                                                     >  1:8090821/1‑140 (MQ=255)

                                                                                     tCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATa                                                   >  2:4990485/1‑140 (MQ=255)

                                                                                       gcgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATaaa                                                 >  1:7907789/1‑140 (MQ=255)

                                                                                       gcgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATaaa                                                 >  2:6846237/1‑140 (MQ=255)

                                                                                       gcgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATaaa                                                 >  2:6121853/1‑140 (MQ=255)

                                                                                       gcgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATaaa                                                 >  2:4821184/1‑140 (MQ=255)

                                                                                        cgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAAt                                                >  2:3022745/1‑140 (MQ=255)

                                                                                        cgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAAt                                                >  1:8662791/1‑140 (MQ=255)

                                                                                        cgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAAt                                                >  1:4069783/1‑140 (MQ=255)

                                                                                        cgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAAt                                                >  1:4289011/1‑140 (MQ=255)

                                                                                           aGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGc                                             <  1:4160275/140‑1 (MQ=255)

                                                                                            gTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCg                                            >  1:9386434/1‑140 (MQ=255)

                                                                                            gTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCg                                            >  1:3493962/1‑140 (MQ=255)

                                                                                             ttCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGt                                           >  1:826250/1‑140 (MQ=255)

                                                                                                ggATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTgcg                                        <  2:9008273/140‑1 (MQ=255)

                                                                                                ggATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTgcg                                        <  1:5719173/140‑1 (MQ=255)

                                                                                                ggATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTgcg                                        <  1:2454755/140‑1 (MQ=255)

                                                                                                     aGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCAttt                                   <  2:5642762/140‑1 (MQ=255)

                                                                                                      ggCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTtc                                  >  2:7613525/1‑140 (MQ=255)

                                                                                                       gCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTtct                                 >  1:5441228/1‑140 (MQ=255)

                                                                                                       gCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTtct                                 >  2:1704410/1‑140 (MQ=255)

                                                                                                               gccgcATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAg                         <  1:7673536/140‑1 (MQ=255)

                                                                                                                   cATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGc                     <  1:60361/140‑1 (MQ=255)

                                                                                                                     tCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCtt                   <  1:6816822/140‑1 (MQ=255)

                                                                                                                       cGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCa                 >  2:9252012/1‑140 (MQ=255)

                                                                                                                              aaaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaa          <  1:4682657/140‑1 (MQ=255)

                                                                                                                              aaaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaa          <  1:9136487/140‑1 (MQ=255)

                                                                                                                                acacCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAAc        >  1:4330078/1‑140 (MQ=255)

                                                                                                                                    cTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGt                                                                                             >  2:5321045/1‑51 (MQ=255)

                                                                                                                                     ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGcccc   <  1:3199070/140‑1 (MQ=255)

                                                                                                                                     ttATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGcccc   >  1:6408455/1‑140 (MQ=255)

                                                                                                                                      tATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCca  <  1:8817983/140‑1 (MQ=255)

                                                                                                                                      tATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCca  <  1:2134986/140‑1 (MQ=255)

                                                                                                                                      tATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCca  <  2:3609590/140‑1 (MQ=255)

GATGTGCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCCA  >  NZ_CP025268/2621962‑2622235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`