BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,654,514	T→C	M600V (ATG→GTG)	yfhM ←	alpha2‑macroglobulin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,654,514	0	T	C	99.6%	1851.0 / NA	533	M600V (ATG→GTG)	yfhM	alpha2‑macroglobulin
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (295/235); minor base G (1/1); total (297/236)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 533 total aligned reads displayed.

TGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCATCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAGCGTG  >  NZ_CP025268/2654382‑2654650

    ttCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGc                                                                                                                               >  1:5519150/1‑140 (MQ=255)

      catcaCCAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGccc                                                                                                                             <  1:8325453/140‑1 (MQ=255)

      catcaACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGccc                                                                                                                             <  2:6542695/140‑1 (MQ=255)

      catcaACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGccc                                                                                                                             >  2:4862238/1‑140 (MQ=255)

         caacaaTGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGAt                                                                                                                          <  1:6066375/140‑1 (MQ=255)

           acaaTGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTc                                                                                                                        <  2:2570504/140‑1 (MQ=255)

             aaTGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCa                                                                                                                      <  1:9145355/140‑1 (MQ=255)

              aTGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAg                                                                                                                     >  1:2756567/1‑140 (MQ=255)

               tGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCNCGTCACCGGGCGACCGCCCGATTCCAGc                                                                                                                    >  1:6280643/1‑140 (MQ=255)

                 ggCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCTGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGCTTCCAGCaa                                                                                                                  >  2:8555012/1‑140 (MQ=255)

                 ggCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCaa                                                                                                                  >  2:4442430/1‑140 (MQ=255)

                     gTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTa                                                                                                              <  1:1647347/140‑1 (MQ=255)

                     gTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTa                                                                                                              <  1:5071542/140‑1 (MQ=255)

                      tttCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTAc                                                                                                             >  2:5385531/1‑140 (MQ=255)

                      tttCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTAc                                                                                                             >  1:4247090/1‑140 (MQ=255)

                         cACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCt                                                                                                          <  1:3388724/140‑1 (MQ=255)

                          aCCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTg                                                                                                         <  2:5655555/140‑1 (MQ=255)

                              tGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGaaa                                                                                                     >  1:796497/1‑140 (MQ=255)

                              tGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGaaa                                                                                                     >  1:8480217/1‑140 (MQ=255)

                              tGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGaaa                                                                                                     <  2:7432866/140‑1 (MQ=255)

                               gCTGTCAGGACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTCCCCTGGaaaa                                                                                                    <  2:6050713/140‑1 (MQ=255)

                                cTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAAt                                                                                                   >  2:7163281/1‑140 (MQ=255)

                                cTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAAt                                                                                                   >  2:5199338/1‑140 (MQ=255)

                                cTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAAt                                                                                                   <  1:7678880/140‑1 (MQ=255)

                                cTGTCAGTACGATAATCGTAAACCGATTACGAGGCGAACTGCGGACGGATCACCGGCACTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGCCCGCCCGATTCCAGCCAACTCCCCTGGAAAAt                                                                                                   >  2:9095215/1‑140 (MQ=255)

                                   tCAGTTCGATAATCGTAACCCGAGTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGTGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                                <  2:3686368/140‑1 (MQ=255)

                                   tCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                                >  1:8979160/1‑140 (MQ=255)

                                   tCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                                <  2:1091802/140‑1 (MQ=255)

                                   tCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                                <  1:2673786/140‑1 (MQ=255)

                                   tCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                                <  2:3008705/140‑1 (MQ=255)

                                     aGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCt                                                                                              >  1:4514406/1‑140 (MQ=255)

                                     aGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCt                                                                                              <  2:6171586/140‑1 (MQ=255)

                                       tACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGt                                                                                            <  2:2065268/140‑1 (MQ=255)

                                         cGATAATCGTAAACCGATTTCGAGGCGAAGTGCGGACGGATCCCCGGCAATGGATCGGCAGGCCAGATAGCCTTCTCAGCTCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATATCCTGTaa                                                                                          >  2:8462561/1‑140 (MQ=255)

                                          gATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAAt                                                                                         >  2:634237/1‑140 (MQ=255)

                                             aaTCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATggg                                                                                      >  2:9712377/1‑140 (MQ=255)

                                             aaTCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATggg                                                                                      >  2:6444609/1‑140 (MQ=255)

                                             aaTCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATggg                                                                                      <  1:3756226/140‑1 (MQ=255)

                                             aaTCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATggg                                                                                      >  1:3887584/1‑140 (MQ=255)

                                              aTCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATgggg                                                                                     <  2:4940978/140‑1 (MQ=255)

                                               tCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGa                                                                                    <  1:3212816/140‑1 (MQ=255)

                                               tCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGa                                                                                    >  1:4861167/1‑140 (MQ=255)

                                                 gTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAAt                                                                                  >  1:2463646/1‑140 (MQ=255)

                                                 gTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAAt                                                                                  >  1:129248/1‑140 (MQ=255)

                                                 gTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAAt                                                                                  >  1:8854919/1‑140 (MQ=255)

                                                 gTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAAt                                                                                  >  1:6679909/1‑140 (MQ=255)

                                                    aaCCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCg                                                                               <  2:6784662/140‑1 (MQ=255)

                                                    aaCCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCg                                                                               >  2:2854997/1‑140 (MQ=255)

                                                    aaCCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCg                                                                               <  2:1445609/140‑1 (MQ=255)

                                                         aTTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTttcc                                                                          >  1:7843878/1‑140 (MQ=255)

                                                         aTTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTttcc                                                                          >  2:151811/1‑140 (MQ=255)

                                                               aGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCAc                                                                    <  2:2324092/140‑1 (MQ=255)

                                                               aGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCAc                                                                    <  1:3009476/140‑1 (MQ=255)

                                                               aGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCGGGAAAATAACCTGTAAGGGGGAATGCGTTTCCTTCCAc                                                                    <  2:7965320/140‑1 (MQ=255)

                                                                    aaCTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCTGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCt                                                               >  1:8146570/1‑140 (MQ=255)

                                                                    aaCTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCt                                                               >  1:7556713/1‑140 (MQ=255)

                                                                    aaCTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCt                                                               <  1:9299408/140‑1 (MQ=255)

                                                                     aCTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCtt                                                              <  1:2276274/140‑1 (MQ=255)

                                                                      cTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCttt                                                             <  2:1668798/140‑1 (MQ=255)

                                                                       tgaggacggaTCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTGCCACTGGCTTTc                                                            <  2:7787751/137‑1 (MQ=255)

                                                                                 tCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACt                                                  <  2:7611505/140‑1 (MQ=255)

                                                                                     cGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTcgc                                              >  1:1099582/1‑140 (MQ=255)

                                                                                       gCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTcgccg                                            <  2:6908773/140‑1 (MQ=255)

                                                                                       gCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTcgccg                                            <  2:8375939/140‑1 (MQ=255)

                                                                                       gCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGCAGAAACTTcgccg                                            <  1:6454221/140‑1 (MQ=255)

                                                                                        cAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTcgccg                                            <  1:944662/139‑1 (MQ=255)

                                                                                          aTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAAAAACCTGTCATGTGGAATGCGTGTCCTGCCACTGGCTTTCTGTAGAAACGTCGCGgcgc                                         >  2:9359012/1‑140 (MQ=255)

                                                                                           tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGTGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                        <  2:412053/140‑1 (MQ=255)

                                                                                           tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                        <  2:3245038/140‑1 (MQ=255)

                                                                                           tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                        >  2:7255477/1‑140 (MQ=255)

                                                                                           tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                        <  2:3347990/140‑1 (MQ=255)

                                                                                           tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                        <  2:5929690/140‑1 (MQ=255)

                                                                                           tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                        <  1:9582798/140‑1 (MQ=255)

                                                                                              aTCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCtt                                     >  1:6876691/1‑140 (MQ=255)

                                                                                                cGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTa                                   >  1:5794439/1‑140 (MQ=255)

                                                                                                    aGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTatcat                               <  2:8186239/140‑1 (MQ=255)

                                                                                                     ggCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTatcatc                              <  1:185170/140‑1 (MQ=255)

                                                                                                     ggCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTatcatc                              >  2:6643488/1‑140 (MQ=255)

                                                                                                      gCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATcc                             >  2:1344404/1‑140 (MQ=255)

                                                                                                      gCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATcc                             >  2:1837635/1‑140 (MQ=255)

                                                                                                         aGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAAGGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGc                          <  2:516351/140‑1 (MQ=255)

                                                                                                           aTAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAACCTTCGCCGCGCCCTTTAGCATCCAGCGt                        >  1:1925637/1‑140 (MQ=255)

                                                                                                                cTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAACCTTCGCCGCGCCCTTTATCATCCAGCGTCAACt                   >  2:8712780/1‑140 (MQ=255)

                                                                                                                cTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACt                   <  1:3526705/140‑1 (MQ=255)

                                                                                                                cTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACt                   <  1:471826/140‑1 (MQ=255)

                                                                                                                cTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACt                   <  1:4614760/140‑1 (MQ=255)

                                                                                                                cTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACt                   <  2:6847626/140‑1 (MQ=255)

                                                                                                                  gCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGACAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCCTCCAGCGTCAACTGa                 >  2:8968697/1‑140 (MQ=255)

                                                                                                                   cTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGaa                >  1:4331922/1‑140 (MQ=255)

                                                                                                                   cTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGaa                >  1:7304013/1‑140 (MQ=255)

                                                                                                                    tCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAAc               <  2:4639201/140‑1 (MQ=255)

                                                                                                                       gcgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTATCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTc            >  2:4305177/1‑140 (MQ=255)

                                                                                                                        cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCa           >  1:5306174/1‑140 (MQ=255)

                                                                                                                        cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCa           >  1:7226607/1‑140 (MQ=255)

                                                                                                                        cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCa           >  2:9540189/1‑140 (MQ=255)

                                                                                                                             cgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAg      >  1:145791/1‑140 (MQ=255)

                                                                                                                              gcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAGc     >  1:3986444/1‑140 (MQ=255)

                                                                                                                              gcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAGc     <  2:120350/140‑1 (MQ=255)

                                                                                                                                 tCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAGCGTg  <  1:2332258/140‑1 (MQ=255)

                                                                                                                                 tCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAGCGTg  >  1:103057/1‑140 (MQ=255)

TGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCATCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAGCGTG  >  NZ_CP025268/2654382‑2654650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`