BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,913,061	T→C	112 (TAA→TAG)	mazF ←	endoribonuclease MazF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,913,061	0	T	C	99.5%	1922.9 / NA	552	112 (TAA→TAG)	mazF	endoribonuclease MazF
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (308/241); minor base A (1/2); total (309/243)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 553 total aligned reads displayed.

ACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCT  >  NZ_CP025268/2912924‑2913197

aCGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTTAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTa                                                                                                                                        >  1:2896926/1‑140 (MQ=255)

 cGGGCAGCCGTTTTCCGGCTCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTAc                                                                                                                                       <  1:9324673/140‑1 (MQ=255)

 cGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTAc                                                                                                                                       <  2:5852885/140‑1 (MQ=255)

  gggCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTAcc                                                                                                                                      <  2:3514800/140‑1 (MQ=255)

      aGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAAt                                                                                                                                  <  1:1908412/140‑1 (MQ=255)

      aGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAAt                                                                                                                                  >  1:4786850/1‑140 (MQ=255)

      aGCCGTTTTCCGGATCGCGCAGGCGCTGCAAAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGTGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAAt                                                                                                                                  <  2:5962144/140‑1 (MQ=255)

       gCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCTGGTCTGTCAGGTGGTAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACACAATc                                                                                                                                 >  2:5107675/1‑140 (MQ=255)

       gCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATc                                                                                                                                 >  2:1943814/1‑140 (MQ=255)

        ccGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATGGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGAACAGACTAGAAGTGAGTTAGTAACACTACCCAATCa                                                                                                                                >  2:1365838/1‑140 (MQ=255)

         cGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAg                                                                                                                               <  1:5075408/140‑1 (MQ=255)

         cGTGTTCCGGATCGCGCAGGCGCTGCATAATCGTGAGCAAACGGTCGATTTGATTCCTCGCCTTGGCCTGAAACTTGCGGGTCTGGCAGGTGGAAACCGGTGACCAGAATAGCCGTGAGTGAGTAACACTACCCAATCAg                                                                                                                               <  2:2217989/140‑1 (MQ=255)

              tCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGt                                                                                                                          <  2:8282482/140‑1 (MQ=255)

                    tggggcAGGCGATGCATACTAGTGAGCTATCGGTCGATTTGATTCATTGATTTGTCCTGAACATTGCGGGTATGTAAGTTGGAATCCTGTGACCAGAATAGAATTGTGTTAGTAACAATACCCTATCAGTTCGTTTAttt                                                                                                                    <  2:8044373/136‑1 (MQ=255)

                    tCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAAttt                                                                                                                    <  1:2555094/140‑1 (MQ=255)

                      gcgcAGGCGCTGCATACTAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTg                                                                                                                  <  1:2635010/140‑1 (MQ=255)

                      gcgcAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTg                                                                                                                  <  2:2794800/140‑1 (MQ=255)

                       cgcAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTgg                                                                                                                 >  2:4096192/1‑140 (MQ=255)

                       cgcAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTgg                                                                                                                 >  2:2631447/1‑140 (MQ=255)

                       cgcAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGGACGTTAATTTTgg                                                                                                                 >  1:2230706/1‑140 (MQ=255)

                            gcgcTGCATAAGAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGACGTGAGTTAGTAACCCTACCCACTCAGTACGTTAATTTTGGCTTTa                                                                                                            <  2:5719975/140‑1 (MQ=255)

                               cTGCATAATCGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTACCACGACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                                                         <  2:8884092/140‑1 (MQ=255)

                               cTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                                                         <  1:6751724/140‑1 (MQ=255)

                               cTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTACTGAAAATTGCGGGTCTGTCAGGTGGAAAACTGTGACCAGAATAGAAGTGAGTTAGTAACACAACCCAAACAGTACGATAATTTTGGCTTTAATg                                                                                                         >  2:6161794/1‑140 (MQ=255)

                                 gCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTCACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAg                                                                                                       >  2:4511942/1‑140 (MQ=255)

                                  catcatCGTGAGCACACGGTCGATTTGATTCATTGAAGTGTCCTGCAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGt                                                                                                      <  2:9326416/140‑1 (MQ=255)

                                  cATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGt                                                                                                      <  2:3243675/140‑1 (MQ=255)

                                      ataGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGTGTTGTa                                                                                                  >  1:9609448/1‑140 (MQ=255)

                                        aGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAAt                                                                                                >  2:4979216/1‑140 (MQ=255)

                                          tGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                                                         <  1:3961533/129‑1 (MQ=255)

                                          tGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATTAGTTGTAATTc                                                                                              >  1:5268978/1‑140 (MQ=255)

                                          tGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAAtta                                                                                              >  2:4280712/1‑139 (MQ=255)

                                          tGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTc                                                                                              >  1:1069023/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCCTTTAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCGGTGACCATAAAAGAAGTGAGTAAGTAACACTACCCCATCATTACGTTAAATTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  1:5854806/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:3030505/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:3583280/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  1:1232716/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:9464481/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  1:5475686/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACAATACCAAATCAGTACGTTAATTTTGTCTTTAATTAGTTGTAATTCct                                                                                            >  2:7343323/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCGGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTAct                                                                                            >  1:2101838/1‑140 (MQ=255)

                                             gCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTTGCTTTAATGAGTTGTAATTCctc                                                                                           <  2:1848529/140‑1 (MQ=255)

                                              cAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGGTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTGTAATGAGTTGTAATTCctct                                                                                          >  2:8419730/1‑140 (MQ=255)

                                                 aCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTTCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTggg                                                                                       >  1:9222038/1‑140 (MQ=255)

                                                 aCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTggg                                                                                       <  1:3768613/140‑1 (MQ=255)

                                                   ggTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGc                                                                                     >  2:2546802/1‑140 (MQ=255)

                                                     tCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCaa                                                                                   <  2:2975202/140‑1 (MQ=255)

                                                        aTTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTg                                                                                <  1:9466772/140‑1 (MQ=255)

                                                           tGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTCCGTTAATTATGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTc                                                                             >  2:8654849/1‑140 (MQ=255)

                                                                   tGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTttcttc                                                                     >  2:5593247/1‑140 (MQ=255)

                                                                   tGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTttcttc                                                                     >  1:1823448/1‑140 (MQ=255)

                                                                          tCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                                                         <  1:3373900/97‑1 (MQ=255)

                                                                          tCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                                                         >  2:3372993/1‑97 (MQ=255)

                                                                          tCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGctc                                                              >  1:6957478/1‑140 (MQ=255)

                                                                          tCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGctc                                                              >  1:1636491/1‑140 (MQ=255)

                                                                               aaaaTTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTaa                                                                                                 >  1:2721232/1‑100 (MQ=255)

                                                                               aaaaTTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTaa                                                                                                 <  2:2719689/100‑1 (MQ=255)

                                                                               aaaaTTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTgg                                                                                        >  2:5398588/1‑109 (MQ=255)

                                                                                  aTTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGcc                                                      <  1:150385/140‑1 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCGAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    >  1:7219312/1‑140 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGGTCCTCTTGCCCg                                                    >  1:8694546/1‑140 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    >  1:3073597/1‑140 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    >  2:9730363/1‑140 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    >  2:9248209/1‑140 (MQ=255)

                                                                                            tGTCAGGTTGAAACCTGTGACCAGAATAGAACTGAGTTAGTAACACTACCCAATCAGTACGTTCAATTGGGCTTTAATGCGATGTAATTCCTCTGGGGCAACTGGTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa                                            <  2:3947852/140‑1 (MQ=255)

                                                                                            tGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa                                            <  1:6178343/140‑1 (MQ=255)

                                                                                            tGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa                                            <  2:7758774/140‑1 (MQ=255)

                                                                                             gTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGAt                                           >  1:7832521/1‑140 (MQ=255)

                                                                                              tCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGt                                                                                                      >  1:2334443/1‑80 (MQ=255)

                                                                                                   tGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGAGAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCATTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACttta                                     >  2:7105645/1‑139 (MQ=255)

                                                                                                    ggAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACttttc                                    <  1:5652481/140‑2 (MQ=255)

                                                                                                        aCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCt                                >  1:4133664/1‑140 (MQ=255)

                                                                                                        aCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCt                                >  1:7450229/1‑140 (MQ=255)

                                                                                                          cTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                              <  2:2373292/140‑1 (MQ=255)

                                                                                                          cTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                              <  2:9505119/140‑1 (MQ=255)

                                                                                                          cTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                              <  1:9040164/140‑1 (MQ=255)

                                                                                                           tgtgACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGAt                             <  1:6551427/140‑1 (MQ=255)

                                                                                                            gtgACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATAATTTTTACCTGATc                            >  2:1672373/1‑140 (MQ=255)

                                                                                                             tgACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCa                           >  2:6477061/1‑140 (MQ=255)

                                                                                                               aCCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTATCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGc                         <  1:5673951/140‑1 (MQ=255)

                                                                                                                    aaTAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAAt                                                                                                          >  2:4940331/1‑54 (MQ=255)

                                                                                                                    aaTAGAAGTGAGTTAGTAACACGACCCACTCAGTACGTTAATTGTGGCTTTAATGAGTTGTACTTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACg                    <  2:8171615/140‑1 (MQ=255)

                                                                                                                    aaTAGAAGTGAGTTAGTAACACGACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACg                    <  2:2102212/140‑1 (MQ=255)

                                                                                                                         aaGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTa                                   <  1:3551174/120‑1 (MQ=255)

                                                                                                                         aaGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACg               >  1:3228718/1‑140 (MQ=255)

                                                                                                                          aGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGc              <  2:1201051/140‑1 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:4525076/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  2:753658/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  2:6672478/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:2433504/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          <  1:600371/140‑1 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:6406564/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  2:1532316/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:9728867/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:8262602/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTACCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  2:9025608/1‑140 (MQ=255)

                                                                                                                                ttAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCAc        >  1:8511633/1‑140 (MQ=255)

                                                                                                                                   gTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGtt     <  2:6836589/140‑1 (MQ=255)

                                                                                                                                   gTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGtt     >  1:7596782/1‑140 (MQ=255)

                                                                                                                                   gTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGtt     >  1:8086377/1‑140 (MQ=255)

ACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCT  >  NZ_CP025268/2912924‑2913197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`