BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,913,722	T→C	745 (TAA→TAG)	relA ←	GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,913,722	0	T	C	99.7%	2292.1 / NA	655	745 (TAA→TAG)	relA	GTP pyrophosphokinase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (551/101); minor base G (2/0); total (554/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 655 total aligned reads displayed.

CGTAGCCGGGATCCGCACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGGTGTCGCTAC  >  NZ_CP025268/2913585‑2913858

  tAGCCGGGATCCGCACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAAc                                                                                                                                      <  1:6020524/140‑1 (MQ=255)

    gCCGGGATCCGCACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTc                                                                                                                                    >  2:2215309/1‑140 (MQ=255)

    gCCGGGATCCGCACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTc                                                                                                                                    >  1:7295177/1‑140 (MQ=255)

         gATCCGCACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTg                                                                                                                               >  1:7827688/1‑140 (MQ=255)

               cACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGGAGATATCAATCTACATTGTAGATAAGAGCAAATTTCGGCCTAACTCCCGTGCAACCg                                                                                                                         >  2:8858992/1‑140 (MQ=255)

                 ccgccgGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGAc                                                                                                                       <  2:7802163/140‑1 (MQ=255)

                    ccgGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACTAGCAAATTTCGGACTAACTCCCGTGCAACCGAcgcg                                                                                                                    >  2:3724509/1‑140 (MQ=255)

                    ccgGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGAcgcg                                                                                                                    >  2:7239374/1‑140 (MQ=255)

                       gTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGt                                                                                                                 >  2:6623280/1‑140 (MQ=255)

                            tttCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATa                                                                                                            <  1:8551388/140‑1 (MQ=255)

                                          tACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTg                                                                                              >  1:3466221/1‑140 (MQ=255)

                                                aCTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAg                                                                                        <  1:30335/140‑1 (MQ=255)

                                                 cTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGt                                                                                       <  2:5946653/140‑1 (MQ=255)

                                                  tgtgGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGt                                                                                            <  2:7772466/134‑1 (MQ=255)

                                                  tgtgGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGtt                                                                                      >  1:4651897/1‑140 (MQ=255)

                                                         cATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAg                                                                               >  2:4611309/1‑140 (MQ=255)

                                                           tcaCCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCACCCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCa                                                                             >  2:1552591/3‑140 (MQ=255)

                                                           tAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCa                                                                             >  1:1011641/1‑140 (MQ=255)

                                                           tAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCa                                                                             >  1:536950/1‑140 (MQ=255)

                                                           tAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGCGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCa                                                                             >  1:5807127/1‑140 (MQ=255)

                                                                 tttCCTCAAACCGCTCTCATTTGGAGATCCAGGATATCTCAATCTACCTTGTAGATCCGAGCAAATTTCGGCGTAACTCCCGTTCAACCGACGCGCGTCGATACCATCCGGCACCTGGTTGCGTTTACCCAGCAcgcgac                                                                       >  2:6396330/1‑138 (MQ=255)

                                                                 tttCCTCAAACCGCTCTCATATGTAGATCCAGTGTCTTTCAATCTACATTGTAGATACGAGCAAATTTCGGCGTAACTCCGGTGCAACCGACGAGCGTCGATAACATCCGGCTCGGGGTTGAGTTTACCCAGCTCGCGcc                                                                       >  2:4376673/1‑140 (MQ=255)

                                                                 tttCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGcc                                                                       >  1:1486276/1‑140 (MQ=255)

                                                                  ttCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACCTTGTAGATACGAGCAAATTTCGCACTAACTCCCGTGCAACCGAAGCGCGTCGATAACATCCGGCACCTGGTTGTGTGTAGCCAGCACGCGccc                                                                      >  2:7615281/1‑140 (MQ=255)

                                                                   tCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGcccc                                                                     >  1:933001/1‑140 (MQ=255)

                                                                     cTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAg                                                                   <  2:2057610/140‑1 (MQ=255)

                                                                         aaCCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACt                                                               >  1:5204857/1‑140 (MQ=255)

                                                                               tATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAgg                                                         >  1:7075111/1‑140 (MQ=255)

                                                                                      tGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCTGGTTGTaca                                                  >  1:8154565/1‑138 (MQ=255)

                                                                                      tGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTaaa                                                  >  2:8311073/1‑140 (MQ=255)

                                                                                      tGTAGATACAGTATATATCAATCTACATTGTAGAGACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTaaa                                                  <  1:3321689/140‑1 (MQ=255)

                                                                                       gTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAAt                                                 <  1:3217202/140‑1 (MQ=255)

                                                                                         aGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAAtct                                               >  2:2570804/1‑140 (MQ=255)

                                                                                         aGATAAAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACACAGCACGCGCCCCAGCACTTGCAGGTTGTAAAact                                               >  1:9801111/1‑140 (MQ=255)

                                                                                           aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCa                                             >  1:488648/1‑140 (MQ=255)

                                                                                           aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCa                                             <  2:5233072/140‑1 (MQ=255)

                                                                                           aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCa                                             >  1:8345107/1‑140 (MQ=255)

                                                                                           aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCa                                             >  1:9209308/1‑140 (MQ=255)

                                                                                             aCAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTGAATCTCAAt                                           >  1:7600006/1‑140 (MQ=255)

                                                                                             aCAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAAt                                           >  2:5096145/1‑140 (MQ=255)

                                                                                               aGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATgg                                         >  1:7513481/1‑140 (MQ=255)

                                                                                               aGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATgg                                         >  1:2760027/1‑140 (MQ=255)

                                                                                                gTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGt                                        >  2:4004534/1‑140 (MQ=255)

                                                                                                gTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGt                                        >  2:6178892/1‑140 (MQ=255)

                                                                                                 tatataTCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCACCCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTc                                       >  1:9851654/1‑140 (MQ=255)

                                                                                                 tatataTCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTc                                       >  1:2104399/1‑140 (MQ=255)

                                                                                                 tatataTCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTc                                       >  1:5511303/1‑140 (MQ=255)

                                                                                                 tatataTCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTc                                       >  1:5137655/1‑140 (MQ=255)

                                                                                                  atatatCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCa                                      >  1:2696722/1‑140 (MQ=255)

                                                                                                   tatatCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCAt                                     >  1:5661689/1‑140 (MQ=255)

                                                                                                      atCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTc                                  >  1:2244911/1‑140 (MQ=255)

                                                                                                      atCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTc                                  >  2:7392203/1‑140 (MQ=255)

                                                                                                      atCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTAACCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTc                                  >  2:1816477/1‑140 (MQ=255)

                                                                                                      atCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCCGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTc                                       >  1:8954136/1‑135 (MQ=255)

                                                                                                      atCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCCGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTc                                       <  2:8954136/135‑1 (MQ=255)

                                                                                                         aaTCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCAcg                                                                           <  2:5447085/96‑1 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCACATGTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  2:9351066/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTACATCTCAATGGTCATGTCGATgg                             >  1:4420570/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  2:3507901/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:1259736/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  2:6747136/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  2:6901105/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             <  2:9047087/140‑1 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:8787836/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:901831/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:2423477/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:3612546/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:3833960/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:420840/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:6757732/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:447585/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  1:5429600/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             <  1:642612/140‑1 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAAGGGTCATGTCGATgg                             >  2:2952946/1‑140 (MQ=255)

                                                                                                           tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCAGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTTCAGGTTGTAAATCTCAATGGTCATGTCGATgg                             >  2:7073534/1‑140 (MQ=255)

                                                                                                             tACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTc                           >  2:1353802/1‑140 (MQ=255)

                                                                                                             tACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTc                           >  2:5548710/1‑140 (MQ=255)

                                                                                                             tACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTc                           >  2:9526615/1‑140 (MQ=255)

                                                                                                               cATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCACCCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGc                         >  2:9696909/1‑140 (MQ=255)

                                                                                                               cATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGc                         >  1:7768436/1‑140 (MQ=255)

                                                                                                                aTTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGcc                        >  2:4092438/1‑140 (MQ=255)

                                                                                                                aTTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGcc                        >  1:7962919/1‑140 (MQ=255)

                                                                                                                  tGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAg                      <  2:2340760/140‑1 (MQ=255)

                                                                                                                    tAGATCCGAGCAAATTTCGGCCTAGCTCCCTTGCAACCGACGCGCGTCGATTACATGCGGCACCTGGTTGAGTTGACCCAGCACGCGCCCCAGCACTTGCGGGTTGTAAGGCTCAGGGGTCGTGGGGATGGTCggggggg                    >  2:281762/1‑134 (MQ=255)

                                                                                                                      gATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGGTGTAAATCTCAATGGTCAGGTCGATGGTCGCCAGTTGc                  >  2:1111923/1‑140 (MQ=255)

                                                                                                                       aTACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCt                 >  1:9677580/1‑140 (MQ=255)

                                                                                                                         aCGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGt               >  2:7951979/1‑140 (MQ=255)

                                                                                                                         aCGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGt               >  2:3242429/1‑140 (MQ=255)

                                                                                                                         aCGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGt               >  2:8537443/1‑140 (MQ=255)

                                                                                                                         aCGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGt               >  2:8112796/1‑140 (MQ=255)

                                                                                                                          cGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGTTCATGTCGATGGTCGCCAGTTGCTGtt              >  1:1791215/1‑140 (MQ=255)

                                                                                                                            aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg            >  2:1225045/1‑140 (MQ=255)

                                                                                                                            aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg            >  2:5714005/1‑140 (MQ=255)

                                                                                                                            aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg            >  2:3838446/1‑140 (MQ=255)

                                                                                                                              cacaTTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGgt          >  2:4150657/4‑140 (MQ=255)

                                                                                                                              cAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGgt          >  1:6135086/1‑140 (MQ=255)

                                                                                                                              cAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGgt          >  1:257486/1‑140 (MQ=255)

                                                                                                                              cAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGgt          >  1:7374756/1‑140 (MQ=255)

                                                                                                                              cAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGgt          <  2:3399055/140‑1 (MQ=255)

                                                                                                                              cAAAATTCGGCCAAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGCTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGGAACTCTCAATGGTCATGACGATGGTAGCCCGTTGCTGTTTGgt          >  2:4783444/1‑140 (MQ=255)

CGTAGCCGGGATCCGCACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGGTGTCGCTAC  >  NZ_CP025268/2913585‑2913858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`