BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,985,593	T→C	279 (TAA→TAG)	kduI ←	5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,985,593	0	T	C	99.8%	2128.5 / NA	612	279 (TAA→TAG)	kduI	5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (305/306); minor base G (1/0); total (306/306)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 612 total aligned reads displayed.

GTCACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCT  >  NZ_CP025268/2985456‑2985729

  cacaGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAgc                                                                                                                                      >  1:6126987/1‑140 (MQ=255)

  cacaGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAgc                                                                                                                                      >  1:1356729/1‑140 (MQ=255)

   acaGCCCGCTTGCGCCAGCCCCACCGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAgcg                                                                                                                                     <  2:3184079/140‑1 (MQ=255)

    caGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAgcgc                                                                                                                                    <  1:560784/140‑1 (MQ=255)

    caGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAgcgc                                                                                                                                    >  2:2017739/1‑140 (MQ=255)

       cccGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCaaa                                                                                                                                 >  2:1102904/1‑140 (MQ=255)

         cGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATc                                                                                                                               >  1:8039003/1‑140 (MQ=255)

           cTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCtt                                                                                                                             <  1:1102904/140‑1 (MQ=255)

           cTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCtt                                                                                                                             >  2:5526400/1‑140 (MQ=255)

            ttGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCttt                                                                                                                            <  1:3156514/140‑1 (MQ=255)

            ttGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCttt                                                                                                                            <  1:9045090/140‑1 (MQ=255)

            ttGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCttt                                                                                                                            <  1:4430513/140‑1 (MQ=255)

              gcgcCAGCCCCAACGCCCTCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTaa                                                                                                                          <  1:9445146/140‑1 (MQ=255)

                gcCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACg                                                                                                                        <  1:6956948/140‑1 (MQ=255)

                gcCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACg                                                                                                                        <  2:2457876/140‑1 (MQ=255)

                 ccAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACgg                                                                                                                       <  1:7019943/140‑1 (MQ=255)

                  cAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGc                                                                                                                      <  1:5377865/140‑1 (MQ=255)

                     ccccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcac                                                                                                                   <  1:5502645/140‑1 (MQ=255)

                      cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCCGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  1:6681747/140‑1 (MQ=255)

                      cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  2:1783566/140‑1 (MQ=255)

                      cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  1:3923615/140‑1 (MQ=255)

                      cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  2:2941747/140‑1 (MQ=255)

                      cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  1:8934058/140‑1 (MQ=255)

                         aaCGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATgg                                                                                                               >  1:4503698/1‑140 (MQ=255)

                           cGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACAGAAACATTACCTTCGAGAGAAAATGAACTTAACATCATAACAATACATTATTCGTTTATTCACACAACTAGCTCAAATCTTTAACGGCCACATGGTc                                                                                                             <  2:9206129/140‑1 (MQ=255)

                             ccATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCa                                                                                                           <  1:27786/140‑1 (MQ=255)

                             ccATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCa                                                                                                           <  1:1219779/140‑1 (MQ=255)

                               aTCCCCTGACCCAGTCCAGTATCACAACCCGCGACGACCGCAACTTTCCCTTCGAGAGAAAATGCTCTTATAAGCATCACAATACCTTATTCGTTTATGCCCACAACGAGCGCAAATCTTTAACGGCCACATGGTCCaga                                                                                                         <  2:4930441/140‑3 (MQ=255)

                                tCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCatct                                                                                                        >  2:9048351/1‑138 (MQ=255)

                                tCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAANACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCatat                                                                                                        <  1:6302864/140‑1 (MQ=255)

                                     tGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATAtcatca                                                                                                   <  1:6441187/140‑1 (MQ=255)

                                       aCCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCaaa                                                                                                 >  1:9575426/1‑140 (MQ=255)

                                            gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCt                                                                                            >  2:5771010/1‑140 (MQ=255)

                                            gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTGTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCt                                                                                            >  2:2223678/1‑140 (MQ=255)

                                            ggccagtATCACAACCAGTGACGACCTCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCTAATCTTTAACGGCCACATGGTCCATATCATCAAAGAACt                                                                                            >  2:5051120/3‑140 (MQ=255)

                                             tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGACAATGCCCTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTCGCGCAAATCTTTAACGGCCGCATGGGCCTTATCATCTAAGACCTg                                                                                           >  2:7983646/1‑140 (MQ=255)

                                             tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGGTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACTTGGTCCATATCATCAAAGACCTg                                                                                           >  1:8319442/1‑140 (MQ=255)

                                                 gtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGttt                                                                                       >  1:9358116/1‑140 (MQ=255)

                                                 gtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGttt                                                                                       >  1:4607536/1‑140 (MQ=255)

                                                       caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCg                                                                                 >  1:2238340/1‑140 (MQ=255)

                                                          ccAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                                              >  1:9683092/1‑140 (MQ=255)

                                                           cAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCa                                                                             <  1:507088/140‑1 (MQ=255)

                                                            aGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCAt                                                                            >  1:7596052/1‑140 (MQ=255)

                                                            aGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCAt                                                                            >  2:5348116/1‑140 (MQ=255)

                                                            aGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCAt                                                                            >  1:1751136/1‑140 (MQ=255)

                                                            aGTGACGACCGCAACTGTTCCTTCGGGTGAGTATGCTCTTATTATCAGAACCTTAGGGTATTGGTGTTTGCCGCCCAGTTGGGCAAATCGTTAGGGGCCACCTGGGCCAGCTCATCAGTGACCTGGTTTTCACCGACCag                                                                            >  2:3043087/1‑139 (MQ=255)

                                                               gacgacCGCATCTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCATATTCGTTTATGCCCACAACTAGCGCAAATCTTTAAAGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                         <  2:4145726/140‑1 (MQ=255)

                                                               gacgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                         <  2:4601990/140‑1 (MQ=255)

                                                               gacgacCGCAACTTTACCTTCGAGAGAAAATGCACTGAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                         <  2:8616149/140‑1 (MQ=255)

                                                                ccgacCGCCACTTTACCATCGAGAGAAAATGCAATTAAAATCCTAACAAAACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCAGGccc                                                                        <  2:6275286/139‑1 (MQ=255)

                                                                  gacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCa                                                                      >  2:7079907/1‑140 (MQ=255)

                                                                   acCGCAACTTTACCTTCGAGAGAAAATGCACTTCAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTAAACGGCCACATGGTCGATATCATCAAAGACCTGGTTTTGACCGACCATGcccccg                                                                     >  2:7869957/1‑138 (MQ=255)

                                                                      gCACCTTTCCCTGCGAGAGAAAATGAACTTACAATCATCACCATACCTTAATCGTTTAGGCCCACAACTATCGCAACTCTTTAACGGCCCCATGGGCCATATCAGCACAGACCTGGTTTTCACCGACCATGCCCCAGCTa                                                                  <  2:2745010/140‑1 (MQ=255)

                                                                      gCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATa                                                                  <  1:7698351/140‑1 (MQ=255)

                                                                       cAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATaa                                                                 <  1:4890143/140‑1 (MQ=255)

                                                                       cAACTTTACCTTCGAGAGAAAATGCAATTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATaa                                                                 <  2:7309152/140‑1 (MQ=255)

                                                                              aCCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGtata                                                          >  2:9717509/1‑140 (MQ=255)

                                                                               ccTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATaa                                                         >  2:6045535/1‑140 (MQ=255)

                                                                                  tCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCt                                                      >  1:1004752/1‑140 (MQ=255)

                                                                                    gagagaAAATGCACTTAAACTCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACCGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCttt                                                    <  1:3369741/140‑1 (MQ=255)

                                                                                           aaTGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTGTTCACCGACCATGCCCCAGATAAAGGTCTCAGCTTTGGTTCCg                                             >  2:3696397/1‑140 (MQ=255)

                                                                                            aTGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGa                                            <  1:2914402/140‑1 (MQ=255)

                                                                                              gCACTTAAACTCATAACAATACCTTATACGTTTATGCCCCCAACTAGCGCAAATCTTTAACGGCCACCTGCTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATTAGATTTGGTTCCGaca                                          <  2:7388035/140‑1 (MQ=255)

                                                                                              gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                          <  1:7418227/140‑1 (MQ=255)

                                                                                              gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCAGATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                          <  1:3567920/140‑1 (MQ=255)

                                                                                               cACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGacac                                         <  2:3328343/140‑1 (MQ=255)

                                                                                                 cTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCg                                       >  1:9797033/1‑140 (MQ=255)

                                                                                                  ttAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCgg                                      >  1:207475/1‑140 (MQ=255)

                                                                                                   tAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGa                                     >  1:588087/1‑140 (MQ=255)

                                                                                                   tAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGa                                     <  1:1881801/140‑1 (MQ=255)

                                                                                                   tAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGa                                     <  2:5656665/140‑1 (MQ=255)

                                                                                                            aaCAATACGTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGTCCACATGGTCCATATCATCATAGACCTGGTTTTCACCGACCATGCCCCAGTGAAAGGTTTCAGCTTTGGTTCCGACACCTGAATGGTTCGa                            >  2:9556530/1‑140 (MQ=255)

                                                                                                            aaCAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                            <  2:6862563/140‑1 (MQ=255)

                                                                                                            aaCAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                            >  1:8834405/1‑140 (MQ=255)

                                                                                                            aaCAATACATTATTCTTTTATGCCCACAACTAGCGCAATTCTTTAACTGCCACATTGTCAATATCATCAAAGACCTGGTTTTCACCGACAATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGACTGGATCGa                            >  2:3939385/1‑140 (MQ=255)

                                                                                                             aCAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGAc                           <  1:2571910/140‑1 (MQ=255)

                                                                                                             aCAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGAc                           <  1:7217390/140‑1 (MQ=255)

                                                                                                             aCAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGAc                           <  2:1327236/140‑1 (MQ=255)

                                                                                                              cAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGAcc                          <  1:3262766/140‑1 (MQ=255)

                                                                                                              cAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGAcc                          <  1:7893429/140‑1 (MQ=255)

                                                                                                              cAATACCTTATTCGATTATGCCCACACCTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGAcc                          <  2:8831433/140‑1 (MQ=255)

                                                                                                                 tACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGc                       <  1:1563086/140‑1 (MQ=255)

                                                                                                                   ccTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTc                     <  1:376518/140‑1 (MQ=255)

                                                                                                                   ccTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTc                     >  1:4247556/1‑140 (MQ=255)

                                                                                                                    cTTAATCGTGTATGACCACCACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGTTATAAGATTTGGTTGCGACACAGGAATGGATCTACCAGATCg                    >  2:8501996/1‑140 (MQ=255)

                                                                                                                      tATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCggg                  >  2:4358828/1‑140 (MQ=255)

                                                                                                                      tATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCggg                  >  2:4006610/1‑140 (MQ=255)

                                                                                                                       cttCGTTTATGCCCACAACTCGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg                 >  2:262818/2‑140 (MQ=255)

                                                                                                                       aTTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGATTTCACCGACCATGCCCCAGATAAACGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg                 >  2:1918494/1‑140 (MQ=255)

                                                                                                                        ttCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGga                >  1:820646/1‑140 (MQ=255)

                                                                                                                        ttCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGga                >  2:8292937/1‑140 (MQ=255)

                                                                                                                        ttCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGga                >  1:8566766/1‑140 (MQ=255)

                                                                                                                         tCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGgag               <  1:4358828/140‑1 (MQ=255)

                                                                                                                            tttATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATc            >  2:7553922/1‑140 (MQ=255)

                                                                                                                             ttATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCa           >  2:4801850/1‑140 (MQ=255)

                                                                                                                                 gCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATCTCATCCAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGc       <  1:4039984/140‑1 (MQ=255)

                                                                                                                                   ccACAACGAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCt     <  1:8760297/140‑1 (MQ=255)

                                                                                                                                     acaACTAGCGCAACTCTTTAACGGCCACATGGTCCATGTCATGAAAGACCTGGTTGTCAGCGACCCTGCCGCAGATAAAGGGATACGCGGTGGGTGCGTCACCGGCCTGGAGGGACGGGCTCGGGGTGATCCCCGCGTgg   >  2:6484077/1‑139 (MQ=255)

                                                                                                                                     acaACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGc   >  1:3483116/1‑140 (MQ=255)

                                                                                                                                      caACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAATGGTATAAGATTTGGTTCCGACAAAGGAATGGTTCGACCAGCTCGGGGAGATCACCGCCTgag  >  2:7634969/1‑138 (MQ=255)

GTCACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCT  >  NZ_CP025268/2985456‑2985729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`