BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,255,705	A→G	234 (TAA→TAG)	yhaK →	pirin family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,255,705	0	A	G	99.8%	1671.8 / NA	481	234 (TAA→TAG)	yhaK	pirin family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (272/208); minor base T (0/1); total (272/209)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 482 total aligned reads displayed.

AAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAGCT  >  NZ_CP025268/3255568‑3255841

aaaTTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTAACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGtt                                                                                                                                        <  1:6557402/140‑1 (MQ=255)

  ttttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGAGGTGATGGGGCGTTTATTAGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGt                                                                                                                                      <  2:3294252/139‑1 (MQ=255)

  aTTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCTCTGCGCGCTTTGCTGATAGATTTGGCTGTGTAGTTGt                                                                                                                                      >  1:7745616/1‑140 (MQ=255)

  aTTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGt                                                                                                                                      <  1:8573444/140‑1 (MQ=255)

   tttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAgttgtt                                                                                                                                     <  2:4212076/140‑1 (MQ=255)

       aTGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTa                                                                                                                                 <  2:5063207/140‑1 (MQ=255)

       aTGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTa                                                                                                                                 <  1:646037/140‑1 (MQ=255)

        tGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTAc                                                                                                                                <  1:8649963/140‑1 (MQ=255)

         gcgcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACa                                                                                                                               >  2:3836642/1‑140 (MQ=255)

         gcgcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACa                                                                                                                               <  2:2248224/140‑1 (MQ=255)

          cgcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAg                                                                                                                              <  2:4870890/140‑1 (MQ=255)

           gcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAgg                                                                                                                             <  2:8782777/140‑1 (MQ=255)

            cTTACGCATCATGAAGAGACAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAggg                                                                                                                            <  2:9053546/140‑1 (MQ=255)

            cTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGAGGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAggg                                                                                                                            <  2:6948624/140‑1 (MQ=255)

               tcGCATCTTGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGTGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGGCTAGGTGTTGGTACCGGgtga                                                                                                                         >  2:7387966/2‑140 (MQ=255)

               aCGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGgaga                                                                                                                         >  1:1557123/1‑140 (MQ=255)

                     catGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTTTTTTTACAGGGAgatgatgt                                                                                                                   >  2:4889429/1‑139 (MQ=255)

                     catGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAgatgatga                                                                                                                   >  1:6725068/1‑140 (MQ=255)

                     catGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAgatgatga                                                                                                                   <  1:5016312/140‑1 (MQ=255)

                      atGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAgatgatgat                                                                                                                  <  2:4738684/140‑1 (MQ=255)

                      atGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAgatgatgat                                                                                                                  <  1:9651872/140‑1 (MQ=255)

                      atGAAGAGAAAGCGGCGCTGACCTGCGGNGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAgatgatgat                                                                                                                  <  1:6280127/140‑1 (MQ=255)

                       tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGatgatgatg                                                                                                                 >  1:332176/1‑140 (MQ=255)

                       tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGatgatgatg                                                                                                                 >  1:7914323/1‑140 (MQ=255)

                       tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGatgatgatg                                                                                                                 >  1:202136/1‑140 (MQ=255)

                       tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGatgatgatg                                                                                                                 >  2:2293335/1‑140 (MQ=255)

                               caGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACTCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTGTTACAGGGAGATGCTGATGAGTacaaa                                                                                                         >  2:8240317/2‑140 (MQ=255)

                               aaGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTaaaaa                                                                                                         >  2:5314460/1‑140 (MQ=255)

                               aaGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTaaaaa                                                                                                         >  2:3723544/1‑140 (MQ=255)

                               aaGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACAGTACGCTGGTTGCCGATTCCCCACTGCGCGCGTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTaaaaa                                                                                                         >  1:296670/1‑140 (MQ=255)

                                     cgcTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGc                                                                                                   >  2:8049207/1‑140 (MQ=255)

                                         gACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTGGCCGATTCCCCACTGGGCGCTTTGCTTATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCaaa                                                                                               <  1:1385081/140‑1 (MQ=255)

                                          aCCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCaaaa                                                                                              >  2:2458493/1‑140 (MQ=255)

                                             tGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAgc                                                                                           >  1:3979801/1‑140 (MQ=255)

                                               cGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAgcgc                                                                                         >  2:9310322/1‑140 (MQ=255)

                                                    aTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGcc                                                                                    >  1:4922825/1‑140 (MQ=255)

                                                         gCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGa                                                                               >  2:3473269/1‑140 (MQ=255)

                                                         gCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGa                                                                               >  1:1616533/1‑140 (MQ=255)

                                                         gCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGa                                                                               >  2:6556791/1‑140 (MQ=255)

                                                           gTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAg                                                                             >  1:2354204/1‑140 (MQ=255)

                                                            tttATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGc                                                                            <  1:6977562/140‑1 (MQ=255)

                                                            tttATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGGAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGc                                                                            <  2:284212/140‑1 (MQ=255)

                                                              tATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGccc                                                                          >  1:9091178/1‑140 (MQ=255)

                                                                   gTGACGCGGCTAACATTACGCGGGTTGCCGATTCCCCACGGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGCTGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgtggt                                                                     <  2:3151936/140‑1 (MQ=255)

                                                                   gTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgtggt                                                                     <  2:8553852/140‑1 (MQ=255)

                                                                        gAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGa                                                                <  1:513741/140‑1 (MQ=255)

                                                                        gAGGCTAACATTACGCTGGTTGCCGAGTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGa                                                                <  2:9534522/140‑1 (MQ=255)

                                                                             tAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGaa                                                           <  1:1454376/140‑1 (MQ=255)

                                                                             tAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGaa                                                           <  2:7106516/140‑1 (MQ=255)

                                                                             tAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGaa                                                           >  1:4127032/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATCGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTACAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  2:8933229/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACTGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  1:8842366/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  1:3850947/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  2:2604806/1‑140 (MQ=255)

                                                                                      gCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGc                                                  >  1:9405722/1‑140 (MQ=255)

                                                                                      gCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGc                                                  >  1:2852751/1‑140 (MQ=255)

                                                                                       cTGGTTGCCGATTCCCCACTGCGCGCGGTGCTGACAGATTTGCCTGTCGAGCTGTATATCCACGGAGATGAGGAAGAGTACACAATTGGCCAACACGCGCCAGCCGGAGAAGCCCGGGGTGGCGCAAGAACCTGCTCGcc                                                 <  2:4106191/140‑2 (MQ=255)

                                                                                         ggTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCAcc                                               >  2:5552705/1‑140 (MQ=255)

                                                                                          gTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACCGGGAGATGATGATGAGTAAAAAAGTGGCCAATAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGATAGAACCTGCTCGCACCg                                              >  2:9172886/1‑140 (MQ=255)

                                                                                          gTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGaa                                                               >  1:3634477/1‑123 (MQ=255)

                                                                                          gTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGaa                                                               <  2:3634477/123‑1 (MQ=255)

                                                                                          gTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCg                                              >  2:113501/1‑140 (MQ=255)

                                                                                             gCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCa                                           >  1:1214396/1‑140 (MQ=255)

                                                                                             gCCGAGTCCCCACTGCGCGCTTGGCTGATATATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAACAAATTGGCCACACAGCGCCAGCCGGTGACGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCa                                           <  2:4540372/140‑1 (MQ=255)

                                                                                              ccGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCaa                                          <  1:5599482/140‑1 (MQ=255)

                                                                                                gATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTCAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCaaaa                                        >  1:5732874/1‑140 (MQ=255)

                                                                                                      tctcttcgcgcTGTGCTGATAGTTTTGCCTGTCTAGTTGTTTTTTGTGTGAGTTGTTGATGATTAAAATATTGGCCATCAAGCGCCTGCCGGTGTAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCGAACTATTTTg                                  <  2:3350270/134‑1 (MQ=255)

                                                                                                           gcgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTAt                             >  1:1088370/1‑140 (MQ=255)

                                                                                                           gcgagcGTTGCTGATAGAATAGCCTTTCTAGTTGTTTTGACAGGGAGATGATGATGAGTAAAAAATTGGACAAAATGCGCCAGCCGGTGTCGCACTTTGAGTCGGAAGAAACTCCTCGCACCGCCAATAAGTTTGGCAAt                             >  2:9704285/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACCGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATGTTGGCTATg                            >  1:8407060/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTACAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:1014743/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAATGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:7131234/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGTACCTGCTCGCACCGCCCAAAATTTTGGCTATg                            >  1:3717228/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:2482725/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            <  1:4543254/140‑1 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:9747104/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  2:5816486/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:7678287/1‑140 (MQ=255)

                                                                                                              ctcTTTTCTGATAGATTTGCCTGTCTAGTTGTATTTACAGTGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCTCACCGCCAAAAATTTTGTCTATGaa                          <  2:7565959/138‑1 (MQ=255)

                                                                                                              cgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGTGATGATGATGAGTAACAAAGTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAGAGACCCTGCTCGCACCGCCCAACATGTTGGcgaggca                          >  2:7170099/1‑134 (MQ=255)

                                                                                                              cgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAACGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGaa                          <  1:5879957/140‑1 (MQ=255)

                                                                                                               gcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAag                         <  1:1845275/140‑1 (MQ=255)

                                                                                                               gcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTGACAGGGAGATGATGATGAGTAAAAAATTGGCCAAACAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAag                         <  2:8515141/140‑1 (MQ=255)

                                                                                                                cTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGACAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAaga                        <  1:4339489/140‑1 (MQ=255)

                                                                                                                   tGCTGATCGATTTGCCTGTCTAGTTGTTTGTACCGGGAGCGGATGATGAGTACAAAATTGGCCAACAAGCGCCAGCAGGAGACGCCCGTGGTGGCGAACGAAACTGCTCGCACCGCCAAAAATTTTGGCGAGGAAGAGAt                     >  2:1830646/1‑140 (MQ=255)

                                                                                                                       gATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAGATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTg                 <  2:6014426/140‑1 (MQ=255)

                                                                                                                         tAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCATAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  2:1024757/140‑1 (MQ=255)

                                                                                                                         tAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAACAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  1:4401423/140‑1 (MQ=255)

                                                                                                                         tAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCTAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  2:6407811/140‑1 (MQ=255)

                                                                                                                         tAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  2:5716658/140‑1 (MQ=255)

                                                                                                                         tAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  2:7856685/140‑1 (MQ=255)

                                                                                                                         tAGATTTGCCTGTCGAGTTGTTTGTACAGGGAGATGCTGATGAGTAACAAATTGGCCAACACGCGCCAGCCGGTGAATCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGATATGTTgag               <  2:1227881/140‑1 (MQ=255)

                                                                                                                         caGATGTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGAAGAGTAACAAATTGGCCAACAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGCACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  1:4838044/139‑1 (MQ=255)

                                                                                                                           gATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgagcg             <  1:5479732/140‑1 (MQ=255)

                                                                                                                              ttGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgagcgagc          <  2:3917028/140‑1 (MQ=255)

                                                                                                                              ttGCCTGTCTAGTTGTCTTTACAGGGAGATTCTGAGGAGTAAAAATTTGGCCAAAAAGCGCCAGCCCGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCACAAATGGGGGCTATGAAGAGATGTTgagcgagc          <  2:6813793/140‑1 (MQ=255)

                                                                                                                               tGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCt         >  1:5285351/1‑140 (MQ=255)

                                                                                                                               tGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCt         >  1:8944371/1‑140 (MQ=255)

                                                                                                                                gCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTCTGAAGAGATGTTGAGCGAGCTg        >  2:2126188/1‑140 (MQ=255)

                                                                                                                                     tCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAGc   >  2:1593692/1‑140 (MQ=255)

AAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAGCT  >  NZ_CP025268/3255568‑3255841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`