BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	345,338	C→T	L17L (TTG→TTA)	yahN ←	LysE family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	345,338	0	C	T	99.5%	2162.2 / NA	553	L17L (TTG→TTA)	yahN	LysE family transporter
Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (337/212); minor base G (2/1); total (339/214)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 553 total aligned reads displayed.

CCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACTAAT  >  NZ_CP025268/345201‑345474

cccAGCGCCACGCCCAGCCCGCACACCACCCCTGCGCGTCGACCGGAAGCCAGGCAGGATTGTACTACCACAAACCGATTGGCTCCCGGATTACAAAAAGTAAGCACGAACAGTCCTACGGTCAGGTACACGGCATGTaa                                                                                                                                        <  2:3538719/140‑1 (MQ=255)

  cAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAg                                                                                                                                      <  1:6675202/140‑1 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                              <  1:6849592/140‑1 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                              >  2:9718982/1‑140 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                              >  1:9589149/1‑140 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                              >  2:2666652/1‑140 (MQ=255)

             ccAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTg                                                                                                                           >  2:7115847/1‑140 (MQ=255)

                gCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGAtt                                                                                                                        <  2:2532359/140‑1 (MQ=255)

                    ggTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCAt                                                                                                                    <  1:715585/140‑1 (MQ=255)

                    ggTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCAt                                                                                                                    <  1:3365279/140‑1 (MQ=255)

                     gTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAATGTAATCACGAACAGTCCTACGGTCAGGAAAACGGCATGTAAAGGATCGATAGTGTTTTCATc                                                                                                                   >  2:9393767/1‑140 (MQ=255)

                     gTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATc                                                                                                                   >  1:2429540/1‑140 (MQ=255)

                         gCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACAACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGACAGGAAAACGGCATGTAAAGGATCCATAGTGATTTCATCCata                                                                                                               >  1:3107358/1‑140 (MQ=255)

                           aCCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCataaa                                                                                                             >  2:8701594/1‑140 (MQ=255)

                           aCCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCataaa                                                                                                             >  1:1777247/1‑140 (MQ=255)

                              ccTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCataaataa                                                                                                          <  2:3671499/140‑1 (MQ=255)

                              ccTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCataaataa                                                                                                          <  1:5053252/140‑1 (MQ=255)

                               cTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAg                                                                                                         >  2:1116869/1‑140 (MQ=255)

                               cTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAg                                                                                                         <  1:8332258/140‑1 (MQ=255)

                               cTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAg                                                                                                         >  1:1479813/1‑140 (MQ=255)

                                tGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCGGGATTAAAAAAAGTCATCACGAACAGTCGTACGGTCAGGGAAAGGGCGTGTAACGGCTCCATAGTGATTTCATCCATAAATAAGt                                                                                                        >  2:8289440/1‑140 (MQ=255)

                                       cGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaactaa                                                                                                 >  1:1308171/1‑140 (MQ=255)

                                       cGACCGGAACCCAGGCTGGATTGTACTACCACAAAGACAAAGGCTCCCCGATTAAAAAAAGTAATCACCAACAGTCCTACGGTCAGGTAAACGGCATGTAAACGATCCATAGTGAATTCATCCATAACTAAGTGaactaa                                                                                                 <  2:2871191/140‑1 (MQ=255)

                                         aCCGGAAGCCAGGCTGGTTTGTACTACCACACAGGGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaactaact                                                                                               <  1:4153282/140‑1 (MQ=255)

                                         aCCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaactaact                                                                                               >  1:5160784/1‑140 (MQ=255)

                                         aCCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaactaact                                                                                               >  2:1767832/1‑140 (MQ=255)

                                                   aGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTACTCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATAtt                                                                                     >  1:7826736/1‑140 (MQ=255)

                                                         gTTTGTACTACCACAAAGAGATTGGCTCCCGTATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTAcc                                                                               <  2:8493246/140‑1 (MQ=255)

                                                         gTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTAcc                                                                               <  1:8453255/140‑1 (MQ=255)

                                                         gTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTAcc                                                                               >  2:4909052/1‑140 (MQ=255)

                                                          tttGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCa                                                                              >  2:5045395/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     >  2:438554/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     >  1:813320/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     >  2:7501676/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     <  1:8588601/140‑1 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     >  2:3052837/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     >  2:4163956/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     >  1:2678134/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     >  2:9499813/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     >  1:2921299/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     >  1:3722881/1‑140 (MQ=255)

                                                                      caAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCggg                                                                  >  1:8676028/1‑140 (MQ=255)

                                                                        aaGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATcc                                                                                                                                 >  1:5580120/1‑75 (MQ=255)

                                                                        aaGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTg                                                                >  2:4634635/1‑140 (MQ=255)

                                                                        aaGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTg                                                                >  2:5736697/1‑140 (MQ=255)

                                                                          gagaTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCg                                                              <  2:5338857/140‑1 (MQ=255)

                                                                           agaTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCga                                                             >  2:1882345/1‑140 (MQ=255)

                                                                           agaTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCga                                                             >  2:5938804/1‑140 (MQ=255)

                                                                           agaTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCga                                                             >  2:7321732/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAATAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  1:4021783/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  1:6581520/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  1:1539090/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  1:2274827/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  1:6922480/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  1:4518076/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  2:2336609/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCAGTTAAAGGATCCATAGTGATTTCAGCCATAAATAAGTGAACTAACTGCATCATATTTCTCCCAAAAATCCTCGGGTGCgaga                                                           >  2:8119796/1‑140 (MQ=255)

                                                                              ttGGCTCCCGTATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagag                                                          <  1:3977055/140‑1 (MQ=255)

                                                                              ttGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagag                                                          >  2:1435696/1‑140 (MQ=255)

                                                                               tcccgcccAGATTAAAAAAAGAAAACACGAACATCCCTACGGTCACCTACACGGCAAGCAAAGTAACCATAGTGATATCCACCATACACAAGTGAACTAACTGCATCATATGTCGACCACACATAAACGGGTGCcacaga                                                         <  2:7936776/135‑1 (MQ=255)

                                                                                ggCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagagag                                                        <  2:2186412/140‑1 (MQ=255)

                                                                                      cGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcaca                                                  >  1:1881897/1‑140 (MQ=255)

                                                                                      cGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcaca                                                  <  1:2193941/140‑1 (MQ=255)

                                                                                            aaaaaaaGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAgtgt                                            >  1:9696543/1‑140 (MQ=255)

                                                                                                            aaCAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACt                            >  1:8865962/1‑140 (MQ=255)

                                                                                                              cAGTCCTCCGGGCAGGGAAACTGCATGGAAAGGATCCATAGTGATTTCATCCATAAATCAGTGAACTAACTGCATCATATGTCTACCAAAAAGAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTgg                          <  2:8924931/140‑1 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                       >  2:5628811/1‑140 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                       >  2:3318634/1‑140 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                       >  1:3606214/1‑140 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                       >  2:399738/1‑140 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                       >  2:6655011/1‑140 (MQ=255)

                                                                                                                  ccTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGtt                      <  1:8281813/140‑1 (MQ=255)

                                                                                                                  ccTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGtt                      <  1:5942539/140‑1 (MQ=255)

                                                                                                                    tACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTa                    <  2:6093124/140‑1 (MQ=255)

                                                                                                                    tACGGTAAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTTCTGGCGTTTa                    <  1:9467910/140‑1 (MQ=255)

                                                                                                                    caCGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTa                    <  1:7529290/139‑1 (MQ=255)

                                                                                                                     aCGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgag                                                            >  1:2590372/1‑99 (MQ=255)

                                                                                                                       ggTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGc                 <  1:103144/140‑1 (MQ=255)

                                                                                                                        gTCAGGTAAACGGCATGTAACGGATCCAGAGTGATTTCAACCATCAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGcc                <  2:628340/140‑1 (MQ=255)

                                                                                                                        gTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGcc                <  1:1186220/140‑1 (MQ=255)

                                                                                                                        gTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGcc                <  1:7728450/140‑1 (MQ=255)

                                                                                                                           aGGTAAACGGCATNTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:6255993/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCataaata                                                                                                           >  2:7725522/1‑46 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACaa                                                 <  1:532386/104‑1 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:6451132/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:1617091/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:4277239/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  2:9017446/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:4697628/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:5781171/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:8933727/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:9087855/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  2:101487/1‑140 (MQ=255)

                                                                                                                            ggTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctga            <  2:1606764/140‑1 (MQ=255)

                                                                                                                            ggTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGATTATGCCctga            <  2:5160784/140‑1 (MQ=255)

                                                                                                                             gTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaactaac                                                                                                >  1:9255734/1‑55 (MQ=255)

                                                                                                                              tAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctgact          <  2:6917707/140‑1 (MQ=255)

                                                                                                                                  cGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAAc      <  1:4909052/140‑1 (MQ=255)

                                                                                                                                     cATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACTaa   >  2:6455603/1‑140 (MQ=255)

                                                                                                                                      aTGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACTAat  <  1:2820372/140‑1 (MQ=255)

CCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACTAAT  >  NZ_CP025268/345201‑345474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`