BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,608,154	G→A	noncoding (48/87 nt)	CXP41_RS23870 ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,608,154	0	G	A	96.2%	1141.2 / ‑3.1	348	noncoding (48/87 nt)	CXP41_RS23870	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base G (2/0); major base A (181/148); minor base T (6/7); total (189/159)
Fisher's exact test for biased strand distribution p-value = 5.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 348 total aligned reads displayed.

CCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC  >  NZ_CP025268/4608017‑4608290

   ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                      <  1:6600522/140‑1 (MQ=255)

   ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                      <  1:3879608/140‑1 (MQ=255)

   ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                      <  1:2271716/140‑1 (MQ=255)

   ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTATACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                      <  1:2392340/140‑1 (MQ=255)

   ccACGTCCGTAAGGAAACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                      <  2:5791032/140‑1 (MQ=255)

        tCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTAATAGATATCGACTCTTTTTGTGCGAGGGGTGGGACTTGAACCACCGCGTCCGTAAGAACACAAacac                                                                                                                                 >  1:4602957/1‑140 (MQ=21)

         ccGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                                                                <  1:71528/140‑1 (MQ=255)

          cGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                                                                                               >  2:4958189/1‑140 (MQ=255)

                gACACTAACACCTGAAGCTAGCGCGTCTTCAAATTCCGCCACCTTAGCACATTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt                                                                                                                         <  2:2466571/140‑1 (MQ=37)

                 acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                                                                        <  1:1342342/140‑1 (MQ=255)

                 acacTAACACCTCCAGCTAGCGCGACTTCCAATACCTCCACCGACTCACACTCATATTACTATCTTTTATAACGCGTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGTACACTAACACCTGAAGCTa                                                                                                                        <  2:2112594/140‑1 (MQ=12)

                   acTAACACCTGAAGCTATCGCGTCTACCAATTCCGCCACCTTCGAACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACAAACACCTGAAGCTAgc                                                                                                                      >  2:3464723/1‑140 (MQ=255)

                   acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                                                                                                      >  1:6976643/1‑140 (MQ=255)

                   acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATATTACTTTTTTTGTTATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGACACCACAGGTCCGTAAGAACACTAACAGGTGtagctagc                                                                                                                      >  2:5488773/1‑140 (MQ=25)

                   acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAGATTACTTTAAAAGAGATCGAATCGGGGGGTGCGAGGGGGGGGACTTGAACCACCTCGTCCCTAAGAACACAAAAACCTGACGCTAgc                                                                                                                      >  2:1367778/1‑140 (MQ=16)

                    cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                                                                                                     <  2:2364013/140‑1 (MQ=255)

                     tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcgc                                                                                                                    <  1:3126992/140‑1 (MQ=255)

                     tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcgc                                                                                                                    >  2:1793076/1‑140 (MQ=255)

                      aaatccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                                                                   <  2:9700625/136‑1 (MQ=255)

                      aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                                                                   <  2:8538830/140‑1 (MQ=255)

                      aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                                                                   <  2:9273163/140‑1 (MQ=255)

                      aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                                                                   <  1:4341428/140‑1 (MQ=255)

                       acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt                                                                                                                  >  1:2143716/1‑140 (MQ=255)

                         acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGGTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                                                                                                >  2:5887093/1‑140 (MQ=255)

                          ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                                                                                               >  1:7951679/1‑140 (MQ=255)

                          ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                                                                                               <  1:4829903/140‑1 (MQ=255)

                           cTGAAGCTAGCGCGTCTACCACTTCCGCCTCCTTCGCACAGTCATCTTACTTTTTTTGATATCTCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCTCGTCCGTAAGGACAGTAACACCTGAAGCTAGCGGGTCTAc                                                                                                              >  2:4843660/1‑140 (MQ=25)

                            tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATTTCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                                                             <  2:6697605/140‑1 (MQ=255)

                            tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                                                             <  1:5367592/140‑1 (MQ=255)

                             gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                            >  1:4269928/1‑140 (MQ=255)

                             gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                            >  1:3149281/1‑140 (MQ=255)

                             gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGATATCTTACTTTTTTTGATATCGCCTCTTTTGGTGCGAGGGGTGTGACTTGAACCCCCACGTCCTTAAGAACACTAACACCTGAATCTAGATAGTCTACCa                                                                                                            >  2:1930616/1‑140 (MQ=25)

                              aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                                                                                           <  1:4966424/140‑1 (MQ=255)

                              aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                                                                                           >  1:9849384/1‑140 (MQ=255)

                               aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCAAGGGGGGGGACTTGTACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                                                          >  2:6525246/1‑140 (MQ=255)

                                 cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAGCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                                                                                        >  1:5794991/1‑140 (MQ=255)

                                   aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGTGGGGGGACTTGAACCCCCACGTACGTAAGAACACTAACACCTGAATCTAGCGCGTCTACCAATTCCg                                                                                                      >  2:2697112/1‑140 (MQ=255)

                                   aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGGACCCCCACGTCCGTAAGAACACTAACNCCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                      >  1:6276685/1‑140 (MQ=255)

                                   aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTCCCAATTCCg                                                                                                      >  1:8514564/1‑140 (MQ=255)

                                   aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                      >  1:7792020/1‑140 (MQ=255)

                                    gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCAACTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                     <  1:3714538/140‑1 (MQ=255)

                                        gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTATTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCACTTCCGCCAcc                                                                                                 <  2:5580645/140‑1 (MQ=255)

                                         tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                                                                                                >  1:8585903/1‑140 (MQ=255)

                                         tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                                                                                                >  1:9612263/1‑140 (MQ=255)

                                            aCCAATTCCGCCACCTTCTCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                                                             <  1:501339/140‑1 (MQ=255)

                                             ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACTCTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                                                                                            <  2:783136/140‑1 (MQ=255)

                                              cAATTCCGCCACCTTCTCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                           <  2:5930358/140‑1 (MQ=255)

                                              cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                           <  1:4888374/140‑1 (MQ=255)

                                              cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                           <  2:730572/140‑1 (MQ=255)

                                              cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                           <  2:8000942/140‑1 (MQ=255)

                                              cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                           <  1:8337988/140‑1 (MQ=255)

                                              cAATTCCGCCACCTTCGCACAGTCATCTTACATTTTTTGATATCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                           <  2:3982566/140‑1 (MQ=255)

                                              cAATTCCGCCACCTACTCACAGTCATCTTACTTTTATTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                           <  2:703659/140‑1 (MQ=255)

                                                   ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                                      <  1:9182454/140‑1 (MQ=255)

                                                    cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc                                                                                     >  1:6377349/1‑140 (MQ=255)

                                                      ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa                                                                                   >  2:1218023/1‑140 (MQ=255)

                                                      ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa                                                                                   >  1:7291524/1‑140 (MQ=255)

                                                            tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTGCGAGCAata                                                                             >  2:3709665/1‑140 (MQ=255)

                                                              gCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATc                                                                           >  1:7024892/1‑140 (MQ=255)

                                                                 caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                                        <  1:8816674/140‑1 (MQ=255)

                                                                    tCATCTTACTTTTTTTGATATCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGAAATATCAACGTg                                                                     >  1:4639964/1‑140 (MQ=255)

                                                                    tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCCCTATCCACGTg                                                                     >  1:1656527/1‑140 (MQ=255)

                                                                    tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGAACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                                                     >  1:2899797/1‑140 (MQ=255)

                                                                    tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGAGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                                                     >  1:2216562/1‑140 (MQ=255)

                                                                     cATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCa                                                                                <  1:4054736/128‑1 (MQ=255)

                                                                     cATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACgggg                                                                    >  1:2048779/1‑140 (MQ=255)

                                                                      aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAATATCAACGTGGt                                                                   >  2:6176614/1‑140 (MQ=255)

                                                                       tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTGGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGAGAGCAATATCAAAGTGGtt                                                                  >  2:9422953/1‑140 (MQ=255)

                                                                        cTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttt                                                                 >  1:7438755/1‑140 (MQ=255)

                                                                              ttttttGAAATCGACTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCAGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                           <  2:7442279/140‑1 (MQ=18)

                                                                                 tttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTGTTGGTGCGa                                                        >  2:862148/1‑140 (MQ=255)

                                                                                      tatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑ggggg                                                  <  1:8345383/140‑1 (MQ=255)

                                                                                         cGCCTTTTTTGGTGCTAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTATACCAATTCCTACACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gggggggg                                               <  2:4693929/140‑1 (MQ=21)

                                                                                         cGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gggggggg                                               >  2:2727268/1‑140 (MQ=255)

                                                                                           ccTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATAAACGTGGTTTTTGGTGCGA‑GGGGGGGGAc                                             >  2:4170336/1‑140 (MQ=255)

                                                                                               gTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCTTCAGAACACTCACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCGTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGa                                         <  2:7398596/140‑1 (MQ=21)

                                                                                               atttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTTCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGa                                         <  2:4891646/139‑1 (MQ=255)

                                                                                                tttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGaa                                        >  1:1137752/1‑140 (MQ=255)

                                                                                                      gCGAGGGGGTGGACTTGCCCCCCCACTTCCGTATGAAAACTAACACCTGAAGCTAGAGCGTATACCTATTCCGCAACATTAGCCAATTGCGAGAAATATAAAAGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCa                                  <  2:3241361/140‑1 (MQ=16)

                                                                                                        gtggggggggACCTGATCCCCCCCGTCCGTAAGCACTCTAACACCTGCAGCTTTCGCGTCGACCAATTCCGCCACCTTCGCCCAGTGCGAGAAAGATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACg                                <  2:4335581/138‑1 (MQ=21)

                                                                                                        gAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCTATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACg                                <  2:6467977/140‑1 (MQ=255)

                                                                                                          ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCCAGTCCGCCACCTTCGCCCAGTGCGAGCAATATAAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTc                              <  1:142092/140‑1 (MQ=255)

                                                                                                             gggggACTTGAACCCCCACGTCCGTAAGCACCCTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCCGTGCTAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGt                           <  2:3422427/140‑1 (MQ=255)

                                                                                                               gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTac                         >  1:8741100/1‑139 (MQ=255)

                                                                                                               gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTaa                         >  1:3408864/1‑140 (MQ=255)

                                                                                                                gtaCTTGACCCCCCACGTCCGTAAGTCCACTCCCTGCTGTCGCTTGCGCGTCTACACATTCCGCCACGTTCGCACATAGCGAGATATATCAACGTGGTTTTTGGGGCGT‑GGGGGGGGACTTGAACCCCCACGTCCGTAAg                        <  2:8940099/138‑1 (MQ=19)

                                                                                                                gtaCTTGAACCCCCACGTCCTTAAGAACACTAACACCTGAATCTACCGCGTATACCAATTCCGCCAACCTCGCCCAGTGCTAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAg                        <  2:6143788/138‑1 (MQ=25)

                                                                                                                ggAGTTGAACCCCCTGTTTCGTAATTACACTTTCACCTGAAGCTTGCGCGTCTACCTCTTCCTACACCGTCGCCCAGTGCGATTAATTTAATCGTGTTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAg                        <  2:7461550/140‑1 (MQ=16)

                                                                                                                ggACTTGAACCCCCCCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAg                        <  2:1877776/140‑1 (MQ=255)

                                                                                                                 gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCGACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGTTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAgg                       <  2:3554439/140‑1 (MQ=255)

                                                                                                                      gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                  <  2:8782926/140‑1 (MQ=255)

                                                                                                                            ccACGTCTTTAAGAACACTTGCACCTGAATCTTGAGCGTATTCCTTGTCCGCCTCCTTCGCCCATTGCTAGAAATATCAACGAGTTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc            <  2:3087142/140‑1 (MQ=12)

                                                                                                                              aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTATCACCTg          >  2:450999/1‑140 (MQ=255)

                                                                                                                              aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTg          >  1:766203/1‑140 (MQ=255)

                                                                                                                               cGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGa         >  2:1500297/1‑140 (MQ=255)

                                                                                                                                   cGTAAGAACACTAACACCTGAAGCTAGCGCGTCGACCCATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt     >  2:8367869/1‑140 (MQ=255)

                                                                                                                                    gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTGTGGTGCGA‑GGGGGGGGACTTGCACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa    >  1:1946323/1‑140 (MQ=255)

                                                                                                                                     tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa    >  2:9654872/1‑140 (MQ=255)

                                                                                                                                     tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg   >  2:9059142/1‑140 (MQ=255)

                                                                                                                                     tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg   >  1:9326728/1‑140 (MQ=255)

CCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC  >  NZ_CP025268/4608017‑4608290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`