Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,728,808 (C)10→8 pseudogene (1254‑1255/1457 nt) xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,728,8000C.92.7% 549.9 / 35.7 137pseudogene (1263/1457 nt)xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base C (10/0);  new base . (58/68);  total (69/68)
Fisher's exact test for biased strand distribution p-value = 1.37e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NZ_CP0252683,728,8010C.94.3% 483.2 / 41.1 137pseudogene (1262/1457 nt)xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base . (54/63);  total (69/68)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 147 total aligned reads displayed.

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCC  >  NZ_CP025268/3728661‑3728932
                                                                                                                                           ||                                                                                                                                   
ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                      <  2:10340766/140‑1 (MQ=255)
      tAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccc                                                                                                                                <  2:8482504/140‑1 (MQ=255)
         gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGccccccccgg                                                                                                                             >  1:3058744/1‑138 (MQ=255)
          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTccccccccgtg                                                                                                                            >  1:2558270/1‑137 (MQ=255)
          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGccccccccgta                                                                                                                            >  2:6662473/1‑137 (MQ=255)
            gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccgcccgtacg                                                                                                                          >  2:10486432/1‑135 (MQ=255)
                     tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAg                                                                                                               <  1:10031946/140‑1 (MQ=255)
                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCGCGTACGGTGATCGGgcg                                                                                                             >  2:128000/1‑139 (MQ=255)
                          aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                          <  1:6637890/140‑1 (MQ=255)
                          aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                          <  1:2509573/140‑1 (MQ=255)
                             tGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGGACGGTCATCGAGCTGCTGAc                                                                                                       >  1:6643106/1‑140 (MQ=255)
                             tGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCACCACAT‑‑CCCCCACCGTACGGTAATCGAGCTGCTGAc                                                                                                       >  1:6643093/1‑140 (MQ=255)
                                tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                    <  1:20103/140‑1 (MQ=255)
                                    tGCGACTGTTCTANCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTNCGGTCAGCGAGCTGCGGTCCGCTGag                                                                                                >  1:6063563/1‑139 (MQ=255)
                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGT‑‑CCCCCGCCGGAGGGTAATCGAGCTGGAGACCGCTGat                                                                                                >  2:3162584/1‑140 (MQ=255)
                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑GCCCCCCCGTACGGTGATCGAGCTGCTGTCCGCTggt                                                                                                >  1:5632224/1‑138 (MQ=255)
                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTCCGGTGATCGAGCTGCGGGCCGcgggt                                                                                                >  1:5631857/1‑136 (MQ=255)
                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGat                                                                                                <  1:6393589/140‑1 (MQ=255)
                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAGACGCCGCGATCTGCGCCAGCCTTGCGGCGCCCAGTGCTGGCCCCAC‑‑CGCCCCCCCCGTCCGGGCATCGCGCTGCTGACCGCGGag                                                                                                >  2:1778391/1‑139 (MQ=255)
                                        aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCGCCCCCGTACGGGAATCGTGGTGCTGACCGCTGATATcc                                                                                            >  2:5985648/1‑140 (MQ=255)
                                        aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAAGCGAGCTGCGGACCGCTGCTATcc                                                                                            >  2:4898434/1‑140 (MQ=255)
                                          tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                          <  1:3103791/140‑1 (MQ=255)
                                           gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                                                                         <  2:6404556/140‑1 (MQ=255)
                                            ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAAGCGAGCTGCGGACCGCTGAGATCCGCCa                                                                                        >  2:7927219/1‑140 (MQ=255)
                                            ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGGACGGGACTCGAGCTGCTGACCGCTGATATCCGGCa                                                                                        >  2:7927072/1‑140 (MQ=255)
                                             tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTGATCGTGGTGGTGTGCGCTGATATCGGCCAg                                                                                       >  2:4200156/1‑140 (MQ=255)
                                                 aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTTCGGTAATCGAGCTGCTGGCCGCGGGTTTCCGCCCGCCTc                                                                                   >  2:4120130/1‑140 (MQ=255)
                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  1:7103433/140‑1 (MQ=255)
                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  1:10335516/140‑1 (MQ=255)
                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  1:10326946/140‑1 (MQ=255)
                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  1:1185210/140‑1 (MQ=255)
                                                   ggTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  2:1780352/140‑1 (MQ=255)
                                                   ggTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAGTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  2:10969448/140‑1 (MQ=255)
                                                    gttgTTGCGGAAACATTTCAATGAGGGATTTCTTTGGATTCGCCGCGATATGGGCCTGCCTTGCTGCGCCCATTGCGGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                <  1:8057502/137‑1 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGGTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCGCGTACGGTAAGCGAGCTGCTGGCCGCTGATAGCCGCCAGCGTCTGCCg                                                                              >  1:7063579/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAT‑‑CCCCCCCCGTGCGGTACTCGAGCTGCTGACCGCTGATAGCCGCCAGCCTCTGACg                                                                              >  1:10553659/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑GCCCCCCCGGACGGTAATCGAGGTGCGGGCCGCGGAGAGCCGCCCGCATCTGTCg                                                                              >  1:620955/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CGCCCCCCGTGCGGGGGGCGAGCGGGTGACCGCGGGTATCCGCCAGCAGCTGGCg                                                                              >  2:4657744/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CGCCCCCCGGAGGGTAAGGGAGCTGCTGACGGCGGATGTCCGCCGGCATGTGACg                                                                              >  1:9866248/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCGGTGCGGGATTCGGGGGGCTGTCCGCGGATATCCGCCAGCCGCTGACg                                                                              >  2:5312863/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTTCGGTACTCGAGCTGCTGGCCGCTGTGATCCGCGGGCGTCTGTCg                                                                              >  1:10553660/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGTGATCGGGCTGCTGTCCGGGGGTGTCCGCCCGCATCTGagg                                                                              >  1:11148634/1‑138 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGGAAGCGTGCTGCTGACGGCTGATTTCGGCCCGCGTCTGACg                                                                              >  1:3673319/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                              >  2:10325380/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGAGGGTAATCGGGCTGGTGTCCGGTGGTGTCCGCGAGCATCTGTCg                                                                              >  1:5845557/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CGGCCCCCCCGTACGGTCATCGAGGTGCTGAGCGGTGATATCCGGCAGCATCTGACg                                                                              >  2:5489306/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CGCCCCCCCCGTGCGGTGAGCGAGCGGCTGACGGGGGGTGTCCGCCGGCAtgggcgg                                                                              >  1:8126210/1‑134 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCAGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCATCGAGCTGCTGAGCGCTGTTATCCGCCGGCAGCTGACg                                                                              >  1:437458/1‑140 (MQ=255)
                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGT‑CGGTAATCGAGCTGCTGTGCGCTGATATCCGCGTGCATCTGACGc                                                                             >  2:1688740/1‑140 (MQ=255)
                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CGCCCCCCGTTCGGTGATCGAGCGGCTGGGCGCTGAGCTCCGCCAGCAtgtggggc                                                                             >  1:8830732/1‑136 (MQ=255)
                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                             <  1:2210287/140‑1 (MQ=255)
                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTTCGGTAATCGAGCTGCTGTCCGCTGATGTCCGCCCGCATCTGaggg                                                                             >  1:8830717/1‑137 (MQ=255)
                                                        ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CGCCCCCCCCGTGGGGTGATCGGGGTGCTGCGCGCGGGTAGGCGCCAGCAGCTGACGcc                                                                            >  1:2791812/1‑140 (MQ=255)
                                                         tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                           <  1:7690980/140‑1 (MQ=255)
                                                            ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                                                                        <  2:9747421/140‑1 (MQ=255)
                                                             gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc                                                                       >  1:4877588/1‑140 (MQ=255)
                                                              caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                      <  1:922503/140‑1 (MQ=255)
                                                              caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                      <  1:6147498/140‑1 (MQ=255)
                                                               aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTc                                                                     <  1:9328615/140‑1 (MQ=255)
                                                               aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTc                                                                     <  1:4815253/140‑1 (MQ=255)
                                                                    ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATTTCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                >  1:5611514/1‑140 (MQ=255)
                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGGAATCGAGCTGCGGACCGCTGTGGTCCGCCAGCAGCTGAGGCCAGGGCTCTCTACGcgccccg                                                        >  1:8257828/1‑140 (MQ=255)
                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTGATCGAGCTGCTGACCGCTGATATCCGCCCGCATCTGACGCCAGTACTCACGACGcgccccg                                                        >  1:1885069/1‑140 (MQ=255)
                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGGGCGGGACGCGAGCTGCGGGCCGCTGGTGGCCGCCGGCATCTGTCGGCAGTACGCACGACGcgccgcg                                                        >  1:5524079/1‑140 (MQ=255)
                                                                                  tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                                  <  1:4468944/140‑1 (MQ=255)
                                                                                   ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTGCTGGGGCTGCGGTCCGCTGGTGTGCGCGAGCATCTGACGCCAGTGCTGCCTAGGCGCCGCgccgccgc                                                 >  1:8116411/1‑138 (MQ=255)
                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                <  1:4953715/140‑1 (MQ=255)
                                                                                       ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGTAATCGAGCTGCTGTCCGCTGGTTTCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCGCCGATCaa                                             >  1:11075049/1‑140 (MQ=255)
                                                                                       ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGTTCTCCGCCAGCAGCTGACGCCAGGGCTCACTACGCGCCCCGCCCGCAATcca                                             >  2:11116093/1‑138 (MQ=255)
                                                                                       ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGGCGGGCGTCGAGCTGCTGACCGCTGTTATCCGCCCGCAGCTGACGCCAGTGCTCACTACGCGCCCCGCCCCCAATCag                                             >  1:7037094/1‑139 (MQ=255)
                                                                                         aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACg                                           <  1:10955668/140‑1 (MQ=255)
                                                                                          ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGt                                          >  2:10196440/1‑140 (MQ=255)
                                                                                          ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGGACGGGAGTCGAGCTGCTGAGCGCTGATTGCCGCCCGCATCGGACGCGCGTACGCACGACGCGCCCCGCCCCCAAGCAACGt                                          >  1:5783246/1‑140 (MQ=255)
                                                                                              ccgcGATCTGCGCCAGCCATGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTACGCGAGCTGCTGACCGCTGTAATGCGCCCGCTTCTGACGACACTACTCAGGACTCGCCCCGCCCCCAACCCACGGAaca                                      >  2:6689994/1‑140 (MQ=255)
                                                                                                   aTCTGCGCCTGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGACAGCATCTGTCGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                 <  1:2960203/140‑1 (MQ=255)
                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                 <  2:3036253/140‑1 (MQ=255)
                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                 <  1:3505517/140‑1 (MQ=255)
                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                 <  1:3505532/140‑1 (MQ=255)
                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGAGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                 <  2:2368414/140‑1 (MQ=255)
                                                                                                    tCTGCGCCAGCCTTGCTGCGCCCTGTGCTGGCCCCACAT‑CCCCCCCCCGGACGGTCATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCCTTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                 <  2:2742655/140‑1 (MQ=255)
                                                                                                         gacAGCCTTGCTGCGCTCTGTGCTGTCCCCACAT‑‑CCCCCCCCGTACGGTAATCGATCTGCTGACCGCTTATATCCTCAAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCCACGTAACACTTTGCGGttt                           <  2:8523421/138‑1 (MQ=255)
                                                                                                           cAGCCTTGCTGCGCCCCGTGCTGGCCCCACAT‑‑CCCCCCCCGAACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACGACGCGCCCCGCCCCCAATCAACGACACACTTTGCGGTTTaa                         <  2:5471916/140‑1 (MQ=255)
                                                                                                             gCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGAGCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCTAGCAACGGAACACTTTGCGGGTTAATa                       >  1:2508449/1‑140 (MQ=255)
                                                                                                                ttGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGAGATCCGCCAGCATCTGACGCCAGTACTCACGACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                    <  2:584485/140‑1 (MQ=255)
                                                                                                                  gctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTGTGCGGTTTAATACCGCa                  >  2:10956018/1‑140 (MQ=255)
                                                                                                                   ctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTGATCGCGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTGCTCACTACGCGCCCCGCCCCCAAGCAACGTAACACGTTGCGGTGTATTACCGCAg                 >  2:86223/1‑140 (MQ=255)
                                                                                                                   ctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGGCGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTTACACTTTGCGGTTTAATACCGCAg                 >  1:10393477/1‑140 (MQ=255)
                                                                                                                      cgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa              <  2:9572389/140‑1 (MQ=255)
                                                                                                                      cgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa              <  1:8113530/140‑1 (MQ=255)
                                                                                                                      cgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa              <  2:48151/140‑1 (MQ=255)
                                                                                                                      cgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa              <  2:2324010/140‑1 (MQ=255)
                                                                                                                      cgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa              <  1:8113170/140‑1 (MQ=255)
                                                                                                                      cgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCAGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa              <  2:8679112/140‑1 (MQ=255)
                                                                                                                           cgTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc         <  1:7898045/139‑1 (MQ=255)
                                                                                                                            gTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACg        >  2:4582680/1‑140 (MQ=255)
                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCCCTACGCGCCCCGCCCCCAATAAACGTAACACTTTGCGGTTTAATAACGCAGGCATGCACgc       >  2:3590972/1‑139 (MQ=255)
                                                                                                                               cTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa     <  1:11430065/140‑1 (MQ=255)
                                                                                                                                tGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACag    >  2:10429648/1‑139 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGCGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCCGGACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTGTAATACCGCAGGCATGCACGCCATcc  >  1:10262640/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCTGTACTCACTCGGCGCGCCGCGCCCGAGCCGCGTTCGACTTTGGGGTTTAAGACCGGGGGCATGGACgtgatag  >  2:6440661/1‑134 (MQ=255)
                                                                                                                                           ||                                                                                                                                   
GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCC  >  NZ_CP025268/3728661‑3728932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑