BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,629,176	(T)_7→8	pseudogene (141/392 nt)	ydeI ←	YdeI family stress tolerance OB fold protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,629,169	1	.	T	94.7%	1120.7 / 44.6	305	pseudogene (148/392 nt)	ydeI	YdeI family stress tolerance OB fold protein
Reads supporting (aligned to +/- strand): ref base . (0/16); new base T (67/221); total (67/238)
Fisher's exact test for biased strand distribution p-value = 2.73e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 318 total aligned reads displayed.

CGGCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAA  >  NZ_CP025268/1629032‑1629300

    cGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑ttttt‑t                                                                                                                                <  1:243892/140‑1 (MQ=255)

        ggAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑ttttt‑t‑ttgc                                                                                                                           <  2:7162875/140‑4 (MQ=255)

             aaCGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGcatcat                                                                                                                       <  1:5782913/140‑1 (MQ=255)

                   aTTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTc                                                                                                                 >  2:6814097/1‑140 (MQ=255)

                           cACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAg                                                                                                         <  1:559986/140‑1 (MQ=255)

                           cACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAg                                                                                                         <  1:4438043/140‑1 (MQ=255)

                           cACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAg                                                                                                         <  1:8325545/140‑1 (MQ=255)

                            aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGc                                                                                                        >  1:11447944/1‑140 (MQ=255)

                            aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGc                                                                                                        >  2:5469656/1‑140 (MQ=255)

                             ccGCTCTTATCGCGAAACACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGcc                                                                                                       <  2:6572496/140‑1 (MQ=255)

                             ccGCTCTTATCGCGAAAAACGTACCGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGcc                                                                                                       <  1:4069512/140‑1 (MQ=255)

                             ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGcc                                                                                                       <  1:2997985/140‑1 (MQ=255)

                             ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGcc                                                                                                       <  1:6033333/140‑1 (MQ=255)

                             ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGcc                                                                                                       <  1:6780526/140‑1 (MQ=255)

                             ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGcc                                                                                                       <  2:8139698/140‑1 (MQ=255)

                             ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGcc                                                                                                       <  2:2196660/140‑1 (MQ=255)

                               gCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCAt                                                                                                     <  2:6195280/140‑1 (MQ=255)

                               gCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCAt                                                                                                     <  2:1149196/140‑1 (MQ=255)

                                ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCAtc                                                                                                    <  1:6407213/140‑1 (MQ=255)

                                ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCAtc                                                                                                    <  1:9210748/140‑1 (MQ=255)

                                   ttATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCAtctt                                                                                                  <  1:8985338/140‑1 (MQ=255)

                                     aTCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTa                                                                                               <  1:4127292/140‑1 (MQ=255)

                                     aTCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTa                                                                                               >  1:4591189/1‑140 (MQ=255)

                                             aaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGa                                                                                       <  1:5527307/140‑1 (MQ=255)

                                                cGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAggc                                                                                    <  2:2010163/140‑1 (MQ=255)

                                                cGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAggc                                                                                    <  2:136642/140‑1 (MQ=255)

                                                    aCGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGgcggcg                                                                                <  2:9741421/140‑1 (MQ=255)

                                                     cGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGcggcgg                                                                               <  1:1524487/140‑1 (MQ=255)

                                                     cGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGcggcgg                                                                               <  1:8552689/140‑1 (MQ=255)

                                                        tCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTgg                                                                            >  2:5192251/1‑140 (MQ=255)

                                                        tCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTgg                                                                            >  1:1448786/1‑140 (MQ=255)

                                                          ctctCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGgcg                                                                          <  2:1811722/140‑1 (MQ=255)

                                                          ctctCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGgcg                                                                          <  2:10674374/140‑1 (MQ=255)

                                                          cgctcCTTTTTGATAAATCAATTTACCGCGTAGTTAAACCTATGCGCCTTCGGGCATGTTTTTTGGGAAATCAACGTTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGgcg                                                                          <  2:11160480/138‑1 (MQ=255)

                                                            ctcCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCt                                                                        <  2:5567145/140‑1 (MQ=255)

                                                                 ttGTGAGACATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                   <  1:7282938/140‑1 (MQ=255)

                                                                 ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                   <  1:4755003/140‑1 (MQ=255)

                                                                 ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                   <  2:2551659/140‑1 (MQ=255)

                                                                 ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                   <  1:8731166/140‑1 (MQ=255)

                                                                 ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                   <  1:4842960/140‑1 (MQ=255)

                                                                 ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                   <  1:436969/140‑1 (MQ=255)

                                                                 ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                   <  1:698603/140‑1 (MQ=255)

                                                                 ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                   <  1:2127086/140‑1 (MQ=255)

                                                                 ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                   <  2:8023307/140‑1 (MQ=255)

                                                                 ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                   <  1:4218325/140‑1 (MQ=255)

                                                                 gggggagaAATCAAATTACCGCGGAGTGAAACCGACGCGCCATCGGGCAGGGTTTGTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGGTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                   <  2:8355130/136‑1 (MQ=255)

                                                                  tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                  >  2:5829465/1‑140 (MQ=255)

                                                                  tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                  >  1:3855049/1‑140 (MQ=255)

                                                                  tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                  <  1:6682259/140‑1 (MQ=255)

                                                                  tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                  >  2:5335646/1‑140 (MQ=255)

                                                                      agaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGtt                                                              >  1:1093658/1‑140 (MQ=255)

                                                                         aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                   >  2:4197394/1‑132 (MQ=255)

                                                                         aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                   <  1:4197394/132‑1 (MQ=255)

                                                                         aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTa                                                           >  2:9266059/1‑140 (MQ=255)

                                                                         aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTa                                                           >  1:7633347/1‑140 (MQ=255)

                                                                           tCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCACCGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                         <  2:11425161/140‑1 (MQ=255)

                                                                           tCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                         <  2:3800112/140‑1 (MQ=255)

                                                                           tCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                         <  1:1673788/140‑1 (MQ=255)

                                                                            cAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAAc                                                        <  2:1448786/140‑1 (MQ=255)

                                                                              aaTTACCGCGTAGTGACACCGAAGCGCCATCGTGCTTGGTTTTTGCGAAATCATCGGTCAT‑TTTTT‑T‑TGCATCATCTGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGTCGGTGGCGCTGCATCCTGTTTTAAACCg                                                      <  2:4724253/140‑1 (MQ=255)

                                                                                ttACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGcc                                                    <  2:1093506/140‑1 (MQ=255)

                                                                                  aCCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCt                                                  <  1:1240129/140‑1 (MQ=255)

                                                                                    cgcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTgg                                                <  1:10668801/140‑1 (MQ=255)

                                                                                     gcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAAc                                                        >  2:10969591/1‑131 (MQ=255)

                                                                                     gcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAAc                                                        <  1:10969592/131‑1 (MQ=255)

                                                                                      cgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTc                                              <  1:7143254/140‑1 (MQ=255)

                                                                                       gTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCa                                             >  1:7382748/1‑140 (MQ=255)

                                                                                         aGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATc                                           <  2:1198481/140‑1 (MQ=255)

                                                                                         aGTGAAACCGAAGCGCCATCGTGCATGGTATTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATc                                           <  2:3947681/140‑1 (MQ=255)

                                                                                           tGAAACCGAAGCGCCATCGTGCATGGTTTGTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTg                                         <  2:3121383/140‑1 (MQ=255)

                                                                                             aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                       <  2:274331/140‑1 (MQ=255)

                                                                                              aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                      <  1:10162789/140‑1 (MQ=255)

                                                                                              aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                      <  1:3231927/140‑1 (MQ=255)

                                                                                              aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                      <  2:10825976/140‑1 (MQ=255)

                                                                                              aaCCGAAGCGCCATCGAGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTAT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGATTTAAACCGCCCTGGTCATCTGCCa                                      <  2:8827019/140‑1 (MQ=255)

                                                                                                   aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAAc                                                        >  2:7341805/1‑117 (MQ=255)

                                                                                                   aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                                 <  2:4357680/140‑1 (MQ=255)

                                                                                                   aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                                 <  2:3705147/140‑1 (MQ=255)

                                                                                                   aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                                 >  1:1844510/1‑140 (MQ=255)

                                                                                                   aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                                 <  2:7069514/140‑1 (MQ=255)

                                                                                                           tCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                         <  2:4463363/140‑1 (MQ=255)

                                                                                                           tCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                         <  1:6841762/140‑1 (MQ=255)

                                                                                                           acgggCATGGTTTTTGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGGTCCGCGATAGCCCTCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                         <  2:9627145/136‑1 (MQ=255)

                                                                                                                     tttttGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa               <  2:2914980/140‑1 (MQ=255)

                                                                                                                     tttttGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa               <  2:699534/140‑1 (MQ=255)

                                                                                                                     tttttGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa               <  2:6937186/140‑1 (MQ=255)

                                                                                                                     tttttGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa               <  1:7947873/140‑1 (MQ=255)

                                                                                                                     tttttGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa               <  2:10204965/140‑1 (MQ=255)

                                                                                                                     tttttGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa               <  2:1040737/140‑1 (MQ=255)

                                                                                                                     tttgtGCGAAATCACCGGTCA‑‑TTTTT‑TGTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCGGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa               <  2:903847/140‑1 (MQ=255)

                                                                                                                     tatttGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa               <  1:9771311/138‑1 (MQ=255)

                                                                                                                     gttttGCGAAATCTACTGTCAT‑TTTTT‑T‑TGCATCATCGGGTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACATGaaaa               <  2:4605900/139‑1 (MQ=255)

                                                                                                                     gttttGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa               <  2:10599984/139‑1 (MQ=255)

                                                                                                                       tttGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACt             >  1:9539975/1‑140 (MQ=255)

                                                                                                                        ttGCGAAATCAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACtt            >  1:2648923/1‑140 (MQ=255)

                                                                                                                                 cAACGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACa   <  1:617062/140‑1 (MQ=255)

                                                                                                                                 cAAAGGTCA‑‑TTTTT‑TGTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACa   <  2:2811847/140‑1 (MQ=255)

                                                                                                                                  aaCGTTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACaa  <  2:7643701/140‑1 (MQ=255)

                                                                                                                                  aaCGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACaa  <  1:4944941/140‑1 (MQ=255)

                                                                                                                                  aaCGGTCAT‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACaa  <  2:9667060/140‑1 (MQ=255)

CGGCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTT‑T‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAA  >  NZ_CP025268/1629032‑1629300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`