BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,660,178	+C	coding (314/351 nt)	ynfA ←	YnfA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,660,174	1	.	C	99.0%	1792.0 / 6.6	517	coding (318/351 nt)	ynfA	YnfA family protein
Reads supporting (aligned to +/- strand): ref base . (4/1); new base C (232/279); total (236/281)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 548 total aligned reads displayed.

CGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑CCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGC  >  NZ_CP025268/1660036‑1660308

cGAAAAAAGCCGGNGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                        >  1:9852209/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATTGATTTATGTTCCTACGTgcg‑c                                                                                                                                        >  2:3840865/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                        >  1:9497110/1‑140 (MQ=255)

 gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑cc                                                                                                                                       >  1:412433/1‑140 (MQ=255)

   aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑ccc‑c                                                                                                                                    <  1:8854786/140‑1 (MQ=255)

     aaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGACTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑ccc‑cca                                                                                                                                  <  1:10528371/140‑3 (MQ=255)

      aaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑ccc‑ccag                                                                                                                                 <  2:10189948/140‑4 (MQ=255)

        gCCGGAGTGATCACACCAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑ccc‑ccagcc                                                                                                                               <  1:4882567/140‑6 (MQ=255)

         ccGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGccc                                                                                                                               >  2:819183/1‑140 (MQ=255)

          cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCg                                                                                                                              <  1:9202997/140‑1 (MQ=255)

          cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCg                                                                                                                              <  2:2617722/140‑1 (MQ=255)

          cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCg                                                                                                                              <  1:8065307/140‑1 (MQ=255)

             aGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGcaa                                                                                                                           >  2:394644/1‑140 (MQ=255)

               tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGcaaca                                                                                                                         <  1:7116184/140‑1 (MQ=255)

                 aTCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAAt                                                                                                                       >  1:1166963/1‑140 (MQ=255)

                    acaAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGAt                                                                                                                    <  2:5263927/140‑1 (MQ=255)

                    acaAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGAt                                                                                                                    <  2:1555905/140‑1 (MQ=255)

                           aaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCATTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGTATTTATTATCCTACGTGCT‑CCCACAGCCCGCAACAATGATCAACATg                                                                                                             >  1:10313291/1‑140 (MQ=255)

                                     tttGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAgcg                                                                                                   <  1:600192/140‑1 (MQ=255)

                                      ttGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAgcgc                                                                                                  <  1:760245/140‑1 (MQ=255)

                                        gcgcTGTGAATGGTTGACAAAAGATGAAATCGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAgcgcca                                                                                                >  2:9247300/1‑138 (MQ=255)

                                        gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGCTCAACATGCCGCATAGCGCaa                                                                                                >  2:10088865/1‑140 (MQ=255)

                                        gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCaa                                                                                                >  1:8521966/1‑140 (MQ=255)

                                         cgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                               >  1:4398565/1‑140 (MQ=255)

                                           cTGTGAATGGTTGACAAAAGATGAAATAGAAGACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCa                                                                                             <  1:10032068/140‑1 (MQ=255)

                                              tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          <  1:3652455/140‑1 (MQ=255)

                                              tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          <  1:3136164/140‑1 (MQ=255)

                                              tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          >  2:11474412/1‑140 (MQ=255)

                                              tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          >  1:6359490/1‑140 (MQ=255)

                                                aaTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                                        >  2:7231932/1‑140 (MQ=255)

                                                 aTGGTTGACAAAAGATGAACTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                       <  1:10174291/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATTCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  2:10440748/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  2:8647052/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:8545376/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:657237/140‑1 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  2:4838906/1‑140 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:9562425/1‑140 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:7248722/1‑140 (MQ=255)

                                                                tGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACTTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATaaa                                                                        <  1:11025445/140‑1 (MQ=255)

                                                                  aaaTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAaga                                                                      <  2:3647885/140‑1 (MQ=255)

                                                                    ataccaTACCATTTGTCAGCTGACACGTCCTCTAATCAGATTGCTAAAATGGAGTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACCTGCCGCACAGCGCAATCAACGCACCCGTCCAGTCATAAagact                                                                    <  2:237386/135‑3 (MQ=255)

                                                                        aaTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGt                                                                >  1:334459/1‑140 (MQ=255)

                                                                        aaTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGt                                                                >  2:5377031/1‑140 (MQ=255)

                                                                         aTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGtt                                                               >  2:4358969/1‑140 (MQ=255)

                                                                           aCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTc                                                             <  1:1467233/140‑1 (MQ=255)

                                                                           aCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTc                                                             <  2:2878512/140‑1 (MQ=255)

                                                                             cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCAc                                                           <  2:5726545/140‑1 (MQ=255)

                                                                              ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          <  1:2301108/140‑1 (MQ=255)

                                                                              ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          <  2:7541456/140‑1 (MQ=255)

                                                                              ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGCGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          <  2:642825/140‑1 (MQ=255)

                                                                              ttttGTCAGCTGACACGTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACACTGATCACCATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          <  2:5610555/140‑1 (MQ=255)

                                                                               tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                         <  1:2741268/140‑1 (MQ=255)

                                                                                  gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCAt                                                      >  2:3195302/1‑140 (MQ=255)

                                                                                  gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCAt                                                      >  2:10848243/1‑140 (MQ=255)

                                                                                       cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCAc                                                           >  2:9718007/1‑130 (MQ=255)

                                                                                       cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                                 >  1:9360529/1‑140 (MQ=255)

                                                                                       cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                                 >  2:7101875/1‑140 (MQ=255)

                                                                                       cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                                 >  2:1299813/1‑140 (MQ=255)

                                                                                          acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                              <  1:7803512/140‑1 (MQ=255)

                                                                                          acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                              <  2:9439791/140‑1 (MQ=255)

                                                                                          acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                              >  2:1716721/1‑140 (MQ=255)

                                                                                            acTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACACTGCTCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCAGAACGAGTCAGTTGCACGCCATCCACAAcgcg                                            >  1:1806033/1‑140 (MQ=255)

                                                                                            acTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgcg                                            <  1:969146/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:1638913/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:2593667/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:4728525/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  2:4634216/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:5121225/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        >  1:2078026/1‑140 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGGTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  2:959013/140‑1 (MQ=255)

                                                                                                 ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGcc                                       >  2:11100127/1‑140 (MQ=255)

                                                                                                 ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGcc                                       >  2:7880034/1‑140 (MQ=255)

                                                                                                    ttatcttatTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCcaca                                    >  1:2794654/1‑140 (MQ=255)

                                                                                                      atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCCTAAAGAGTCAGTTTCAAGCCATCCACAACGCGCAGCCACATc                                  >  2:6070320/1‑140 (MQ=255)

                                                                                                      atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                                  >  1:7497234/1‑140 (MQ=255)

                                                                                                      atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                                  >  1:7891613/1‑140 (MQ=255)

                                                                                                          tatTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                              >  1:8425508/1‑140 (MQ=255)

                                                                                                          tatTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                              <  1:393880/140‑1 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  1:5704554/1‑140 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  2:1880801/1‑140 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  1:1074263/1‑140 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCAAAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  1:6734471/1‑140 (MQ=255)

                                                                                                            ttGATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTc                                                             >  2:3344707/1‑107 (MQ=255)

                                                                                                              gATAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGt                          >  1:3750506/1‑140 (MQ=255)

                                                                                                               aTAAAATGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGNCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                         <  1:6101786/140‑1 (MQ=255)

                                                                                                               aTAAAATGGATTTATGTTCCAACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                         <  2:6874533/140‑1 (MQ=255)

                                                                                                                 aaaaTGGATTTATGTGCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa                       <  1:4613369/140‑1 (MQ=255)

                                                                                                                   aaTGGATTTATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGa                     <  1:5505554/140‑1 (MQ=255)

                                                                                                                         tttATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATaaa               <  2:8455587/140‑1 (MQ=255)

                                                                                                                         tttATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATaaa               >  2:9038575/1‑140 (MQ=255)

                                                                                                                           tATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             <  2:7703007/140‑1 (MQ=255)

                                                                                                                           tATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             <  1:1904418/140‑1 (MQ=255)

                                                                                                                           tATGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             <  2:8360471/140‑1 (MQ=255)

                                                                                                                            aTGTTCCTACGTGCGCCCC‑CAGCCCGCAACAATGATAAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATACACGc            <  1:1123288/140‑1 (MQ=255)

                                                                                                                             tGTTCCTACGTGCG‑CCA‑CAGCACGAAACAATGATAAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGTGTAAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGcca          >  2:3490885/1‑140 (MQ=255)

                                                                                                                                 cctACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGccacca       >  2:10641858/3‑140 (MQ=255)

                                                                                                                                 cctACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGccacca       >  2:2771487/3‑140 (MQ=255)

                                                                                                                                  ctACGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCAACCACAAAGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCAt      >  2:2078334/2‑140 (MQ=255)

                                                                                                                                    aCGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATaa    <  2:7397258/140‑1 (MQ=255)

                                                                                                                                     cGTGCGCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAg   <  1:7395846/140‑1 (MQ=255)

CGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑CCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGC  >  NZ_CP025268/1660036‑1660308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`