BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,855,625	+C	intergenic (+116/‑50)	sppA → / → ansA	signal peptide peptidase SppA/asparaginase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,855,621	1	.	C	97.9%	2248.2 / 33.6	661	intergenic (+112/‑54)	sppA/ansA	signal peptide peptidase SppA/asparaginase
Reads supporting (aligned to +/- strand): ref base . (5/9); new base C (266/380); total (271/390)
Fisher's exact test for biased strand distribution p-value = 7.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 684 total aligned reads displayed.

TGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑C‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGTG  >  NZ_CP025268/1855483‑1855755

   cTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c‑ccc                                                                                                                                    <  2:4051296/140‑1 (MQ=255)

   cTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c‑ccc                                                                                                                                    <  1:2070000/140‑1 (MQ=255)

       ccTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c‑cccctgc                                                                                                                                <  2:2028276/140‑5 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCt                                                                                                                               <  1:9660441/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCt                                                                                                                               <  1:10497407/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCt                                                                                                                               <  1:323263/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCt                                                                                                                               <  2:8792668/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCt                                                                                                                               <  2:9091588/140‑1 (MQ=255)

          gcgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTc                                                                                                                              >  2:2358401/1‑140 (MQ=255)

          gcgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTc                                                                                                                              >  2:860667/1‑140 (MQ=255)

            gcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCAc                                                                                                                            <  2:185139/140‑1 (MQ=255)

                 cATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGtata                                                                                                                       <  1:1954094/140‑1 (MQ=255)

                  aTGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGtatat                                                                                                                      >  2:10535200/1‑140 (MQ=255)

                     cGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACt                                                                                                                   >  1:2592209/1‑140 (MQ=255)

                      gTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACtt                                                                                                                  <  2:4448696/140‑1 (MQ=255)

                       ttAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACttt                                                                                                                 >  1:7831647/1‑140 (MQ=255)

                            tCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGctc                                                                                                            >  1:6718042/1‑140 (MQ=255)

                            tCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGctc                                                                                                            >  1:4841788/1‑140 (MQ=255)

                               tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCttt                                                                                                         <  2:9508964/140‑1 (MQ=255)

                               tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCttt                                                                                                         <  2:1659289/140‑1 (MQ=255)

                               tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCttt                                                                                                         >  1:950119/1‑140 (MQ=255)

                               tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCttt                                                                                                         >  1:5622682/1‑140 (MQ=255)

                               tGTACTGAGTGGCCGACAGATCGTCGGCCACAATATTTTTTACGTCGACGAATCCTCGTCCCGCTGTTTCGCCCCATATTTCCTTAGCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCttt                                                                                                         <  1:844323/140‑1 (MQ=255)

                                    tGAGTGGCCGACTGAGCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGata                                                                                                    <  2:10115946/140‑1 (MQ=255)

                                      aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                  >  2:772615/1‑140 (MQ=255)

                                      aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                  >  1:1254420/1‑140 (MQ=255)

                                      aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                  >  2:3742567/1‑140 (MQ=255)

                                      aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                  >  2:3580071/1‑140 (MQ=255)

                                        tGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcat                                                                                                >  1:8790010/1‑140 (MQ=255)

                                        tGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcat                                                                                                >  1:2421946/1‑140 (MQ=255)

                                        tGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑CACCCTGCCTCACGTATATACTATTGCTCTATCGATAtcat                                                                                                >  2:11194737/1‑140 (MQ=255)

                                        tGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGAGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcat                                                                                                >  2:8320618/1‑140 (MQ=255)

                                         tgCCGAGAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCGTCCCGCTGTTTCGACCCAGATTTCCTTATGCGCATAGTATCAGGTGCGA‑TCC‑CCCTGCCTCAAGTATATACTTTTGCTCTTTCGTTAtcatt                                                                                               <  2:11067358/139‑1 (MQ=255)

                                         ggCCGTCAGATCGTCGGCCACAATATTTATCACGGCGACCAATCCTCTTCCCGCTGATTCGCCCCACAGCTCCTTATCCGCCTAGTATCAGGCGCGC‑TCC‑CCCTGCCTCCCGTATAGACCTTTGCTCTTTCGATAtcatt                                                                                               <  2:2896660/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  1:10592466/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  2:2673316/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  2:7297197/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  2:5516845/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  1:8956892/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  1:6413842/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGAGAtcatt                                                                                               <  2:3269424/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCGTCCCGCTGTTTCGCCCCATATTTCCTGATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  2:546331/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATATTTTACGTCGACGAATCCTCGTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  1:7200708/140‑1 (MQ=255)

                                          gCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattc                                                                                              >  2:9687292/1‑140 (MQ=255)

                                          gCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattc                                                                                              >  1:9091786/1‑140 (MQ=255)

                                           ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca                                                                                             >  2:7077352/1‑140 (MQ=255)

                                           ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca                                                                                             >  2:2492591/1‑140 (MQ=255)

                                              aCAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatat                                                                                          <  1:7490122/140‑1 (MQ=255)

                                              aCAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatat                                                                                          <  2:11384375/140‑1 (MQ=255)

                                                cgaacgtcgGCCCCAGTTTTTTTTTCGGCCGCGCATCCTCGTCCCCCGGTGTCGCCCCAGATGTACCTATCCGCATAGTATCAGGTGGGCAC‑C‑CCCTGCCTCACGGATATACTTTTGCGCTGTCGCTTTCCGTCatataa                                                                                        >  2:1283725/5‑138 (MQ=255)

                                                     gtcgGCCACGGTATTTTTGCCGGCGTCGAATCCTCTTCCCGCTGTTTCGCCCCATCTTTCCTTATCCGCATAGTAGCAGGTGCGC‑TCC‑CCCTGCCTCGCGTATATACTGTTGCTCTTGCGATATCAGGCatatcaatata                                                                                   >  2:747231/1‑139 (MQ=255)

                                                                tattTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCAGAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGaa                                                                        <  1:7094320/140‑1 (MQ=255)

                                                                      ttACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAAt                                                                  <  1:10328339/140‑1 (MQ=255)

                                                                       tACGACGACGAATCCTCTTCCCGCTGTTTCGCCCCATATGTCCTTATCCGCATAGTAGCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTGTCTATATCATTCATATCAATATCCTGCCAAAGATATCAAtt                                                                 >  2:6370113/1‑140 (MQ=255)

                                                                           tCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTATTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACg                                                             <  1:653564/140‑1 (MQ=255)

                                                                           tCGACGAATCCTCGTCCCGCTGGTTCGCCCCATCTTTCCTTATCCGCATAGTATCAGGGGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACg                                                             <  2:6278997/140‑1 (MQ=255)

                                                                           tCGACGAAGCCTCTGCCCGCTGTTTCGCCCCAGATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCAGATCAATATCATGCAAAAGAAATCAATTTACg                                                             <  2:2142020/140‑1 (MQ=255)

                                                                             gacgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGtt                                                           <  2:8287101/140‑1 (MQ=255)

                                                                               cgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGc                                                         <  2:7193769/140‑1 (MQ=255)

                                                                                   tCCTCGTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCAGTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTac                                                     <  2:7842612/140‑1 (MQ=255)

                                                                                    ccTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTaca                                                    <  1:6225709/140‑1 (MQ=255)

                                                                                        ttCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACAcggg                                                >  1:3987091/1‑140 (MQ=255)

                                                                                        ttCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACAcggg                                                <  1:5942987/140‑1 (MQ=255)

                                                                                          cccGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACAcgggcg                                              >  2:6511540/1‑140 (MQ=255)

                                                                                          cccGCGGTTTCGCCCCATATTTCCTTATCCTCACAGTCTCAGGAGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATCTCATGCAAAAGAAATCAATTTACGTTGCCTACAcgggcg                                              <  2:2986137/140‑1 (MQ=255)

                                                                                           ccGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACAcgggcgg                                             >  1:1421979/1‑140 (MQ=255)

                                                                                              cTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGAc                                          >  1:1692898/1‑140 (MQ=255)

                                                                                                   tCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCg                                     >  1:1003570/1‑140 (MQ=255)

                                                                                                     gCCCCATATTTCCCTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTCTATACTTATGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCggg                                   <  1:6897775/140‑1 (MQ=255)

                                                                                                      ccccATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGa                                  >  1:10788161/1‑140 (MQ=255)

                                                                                                      ccccATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCACTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGa                                  >  1:10172578/1‑140 (MQ=255)

                                                                                                          atatTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCa                              <  1:4454739/140‑1 (MQ=255)

                                                                                                           tatTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAg                             >  1:11354290/1‑140 (MQ=255)

                                                                                                               tCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGtt                         <  1:3364985/140‑1 (MQ=255)

                                                                                                                    aTCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                    <  1:2295725/140‑1 (MQ=255)

                                                                                                                    aTCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                    <  1:2824774/140‑1 (MQ=255)

                                                                                                                    aTCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                    <  1:4135065/140‑1 (MQ=255)

                                                                                                                    aTCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                    >  1:5251483/1‑140 (MQ=255)

                                                                                                                    aTCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                    <  1:8668412/140‑1 (MQ=255)

                                                                                                                     tCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               >  2:8052931/1‑64 (MQ=255)

                                                                                                                     tCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  1:8052932/64‑1 (MQ=255)

                                                                                                                     tCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGc                   >  2:3093943/1‑140 (MQ=255)

                                                                                                                      ccGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  1:11061633/140‑1 (MQ=255)

                                                                                                                      ccGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  1:3731165/140‑1 (MQ=255)

                                                                                                                      ccGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  >  1:997044/1‑140 (MQ=255)

                                                                                                                      ccGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  2:2207430/140‑1 (MQ=255)

                                                                                                                        gCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAgg                >  2:4844451/1‑140 (MQ=255)

                                                                                                                         cATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAggg               >  2:5998292/1‑140 (MQ=255)

                                                                                                                         cATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAggg               <  1:4998673/140‑1 (MQ=255)

                                                                                                                            aGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTta            >  1:6347200/1‑140 (MQ=255)

                                                                                                                                tCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTtatata        >  2:5144051/1‑140 (MQ=255)

                                                                                                                                 ccggTGCGC‑GCC‑CCCTGCCTCACGTCTATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAACAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAACAGGGTTATATAc       <  1:9860764/138‑1 (MQ=255)

                                                                                                                                 cAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATAc       >  1:975177/1‑140 (MQ=255)

                                                                                                                                 cAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATAc       >  1:3593175/1‑140 (MQ=255)

                                                                                                                                 cAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATAc       <  2:1090672/140‑1 (MQ=255)

                                                                                                                                   ggTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCg     >  2:6153554/1‑140 (MQ=255)

                                                                                                                                   ggTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCg     >  1:2943223/1‑140 (MQ=255)

                                                                                                                                    gTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCgg    >  1:700786/1‑140 (MQ=255)

                                                                                                                                    gTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCgg    >  2:4684217/1‑140 (MQ=255)

                                                                                                                                      gcgc‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGgtg  >  2:3432298/1‑140 (MQ=255)

TGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑C‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGTG  >  NZ_CP025268/1855483‑1855755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`