BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,913,492	+T	coding (88/144 nt)	mgrB ←	PhoP/PhoQ regulator MgrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,913,492	1	.	T	99.3%	2765.5 / 5.9	711	coding (88/144 nt)	mgrB	PhoP/PhoQ regulator MgrB
Reads supporting (aligned to +/- strand): ref base . (3/2); new base T (408/297); total (411/300)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 749 total aligned reads displayed.

TTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTG‑ATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGT  >  NZ_CP025268/1913353‑1913625

    ttCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGAGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTgtatc                                                                                                                                    >  2:10888162/1‑136 (MQ=255)

      cATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCGAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGc                                                                                                                                  <  2:3915353/140‑1 (MQ=255)

        tttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcac                                                                                                                                <  2:6875701/140‑1 (MQ=255)

         ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               <  1:6793012/140‑1 (MQ=255)

         ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               <  1:7091661/140‑1 (MQ=255)

         ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               <  1:3801051/140‑1 (MQ=255)

         ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               <  1:8502176/140‑1 (MQ=255)

         ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               <  1:11423026/140‑1 (MQ=255)

         ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               <  1:1544453/140‑1 (MQ=255)

         ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               <  1:8798120/140‑1 (MQ=255)

           caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatc                                                                                                                             <  2:6527425/140‑1 (MQ=255)

           caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatc                                                                                                                             <  2:3511339/140‑1 (MQ=255)

            accaccTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatca                                                                                                                            <  2:4670874/140‑1 (MQ=255)

            accaccTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatca                                                                                                                            <  1:1817783/140‑1 (MQ=255)

                 ctctGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTg                                                                                                                       >  1:10125262/1‑140 (MQ=255)

                      aGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATAc                                                                                                                  <  1:4158340/140‑1 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  2:10694044/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:10835253/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:4696622/1‑140 (MQ=255)

                             gcgcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGcc                                                                                                           <  2:4142893/140‑1 (MQ=255)

                                cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  1:893874/1‑140 (MQ=255)

                                cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  2:5526957/1‑140 (MQ=255)

                                cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  1:7614901/1‑140 (MQ=255)

                                       aCCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAgcagca                                                                                                 >  1:6437956/1‑140 (MQ=255)

                                        ccACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATAACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAATTTGTACATCCTGTATCGCACATCATGTTGAATACCAGCGCCCAAAGCagcaa                                                                                                >  2:7842012/1‑140 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  2:283659/1‑140 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  2:10227961/1‑140 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  1:4608527/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACACATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:7207533/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:4972298/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:9002526/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:3097766/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:8796310/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:11012850/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:8194733/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:3171262/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:2084722/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:4856574/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:5737559/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:5512367/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:5833608/1‑140 (MQ=255)

                                              ctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCaagcaagc                                                                                          >  1:10314666/1‑140 (MQ=255)

                                                 gagaGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCaa                                                                                       >  2:8533079/1‑140 (MQ=255)

                                                  agagGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAac                                                                                      >  2:5271967/1‑140 (MQ=255)

                                                      gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGTATAAAATGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACATGCGCCCAAAGCAGCAAGCAAGCCAAcacca                                                                                  >  1:1962504/1‑140 (MQ=255)

                                                      gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcacca                                                                                  >  1:5206682/1‑140 (MQ=255)

                                                      gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcacca                                                                                  >  2:10043118/1‑140 (MQ=255)

                                                       aagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcaccac                                                                                 <  1:8000930/140‑1 (MQ=255)

                                                             aTCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAAc                                                                           <  1:7878481/140‑1 (MQ=255)

                                                              tCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGc                                                                                                                                  >  2:6319232/1‑84 (MQ=255)

                                                              tCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAAcc                                                                          >  2:3357834/1‑140 (MQ=255)

                                                              tCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAAcc                                                                          >  1:8691014/1‑140 (MQ=255)

                                                                 aGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGATGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGa                                                                       >  2:11216164/1‑140 (MQ=255)

                                                                    gCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATAc                                                                                                                  <  1:9428786/94‑1 (MQ=255)

                                                                    gCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACg                                                                    >  2:5016110/1‑140 (MQ=255)

                                                                     cTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGa                                                                   <  1:10694045/140‑1 (MQ=255)

                                                                                  atCACCACGGGATAAACTGTTTAATGGCACAAATTCCGCTGTAATATTGTACTTCCTGTATCGCTCATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCTAGCAAACACCACGACAACAAGAACGACCCATCGAACCtg                                                      >  2:748301/1‑139 (MQ=255)

                                                                                  atCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACtt                                                      >  1:2732195/1‑140 (MQ=255)

                                                                                     accacGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACttttt                                                   <  1:3914427/140‑1 (MQ=255)

                                                                                         cGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACt                                               >  1:2925916/1‑140 (MQ=255)

                                                                                           ggATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTcc                                             <  1:1121757/140‑1 (MQ=255)

                                                                                             aTAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCaa                                           >  1:3515346/1‑140 (MQ=255)

                                                                                              tAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAt                                          >  1:4550701/1‑140 (MQ=255)

                                                                                                 aCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtctc                                       >  1:2421490/1‑140 (MQ=255)

                                                                                                 aCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtctc                                       >  2:373292/1‑140 (MQ=255)

                                                                                                 aCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtctc                                       >  2:6045176/1‑140 (MQ=255)

                                                                                                 aCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtctc                                       >  1:8212287/1‑140 (MQ=255)

                                                                                                   tGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCg                                     <  2:3029958/140‑1 (MQ=255)

                                                                                                    ggTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                                    <  2:9268184/140‑1 (MQ=255)

                                                                                                    ggTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                                    <  2:7948919/140‑1 (MQ=255)

                                                                                                     gTTAATTGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCAAGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAACTCCGtt                                   >  2:1514640/1‑140 (MQ=255)

                                                                                                     gTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGtt                                   >  1:10460363/1‑140 (MQ=255)

                                                                                                     gTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGtt                                   >  1:7292129/1‑140 (MQ=255)

                                                                                                     gTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGtt                                   >  2:9490578/1‑140 (MQ=255)

                                                                                                     gTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGtt                                   <  1:4338897/140‑1 (MQ=255)

                                                                                                     gTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTGGCCTACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGACCGACCCCTCGAAACTTTTTCACTCCAATCTCCGtt                                   >  2:1061656/1‑140 (MQ=255)

                                                                                                      ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  >  1:604356/1‑140 (MQ=255)

                                                                                                      ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  >  2:7092646/1‑140 (MQ=255)

                                                                                                      ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  >  1:6186987/1‑140 (MQ=255)

                                                                                                      ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  >  2:9689068/1‑140 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  2:2119155/140‑1 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  2:2560657/140‑1 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  2:1989247/140‑1 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  1:10001681/140‑1 (MQ=255)

                                                                                                          tGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc                              >  2:4427368/1‑140 (MQ=255)

                                                                                                             cacaAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctac                           >  2:11419691/1‑140 (MQ=255)

                                                                                                               caAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctacct                         >  1:1098756/1‑140 (MQ=255)

                                                                                                               caAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctacct                         >  2:7593734/1‑140 (MQ=255)

                                                                                                               caAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctacct                         >  2:1157885/1‑140 (MQ=255)

                                                                                                               caAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctacct                         >  1:9080355/1‑140 (MQ=255)

                                                                                                               caAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctacct                         >  1:9839536/1‑140 (MQ=255)

                                                                                                               caAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               >  2:2434064/1‑134 (MQ=255)

                                                                                                                 aaTTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTa                       <  2:1368321/140‑1 (MQ=255)

                                                                                                                  aTTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTAt                      <  2:1756220/140‑1 (MQ=255)

                                                                                                                      cGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa                  <  1:3249849/140‑1 (MQ=255)

                                                                                                                      cGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa                  <  1:961862/140‑1 (MQ=255)

                                                                                                                       gCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCAt                 >  2:5204410/1‑140 (MQ=255)

                                                                                                                                   tACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATg     >  2:10478300/1‑140 (MQ=255)

                                                                                                                                    aCATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCACTCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGc    <  2:977826/140‑1 (MQ=255)

                                                                                                                                    aCATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGc    <  1:2217202/140‑1 (MQ=255)

TTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTG‑ATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGT  >  NZ_CP025268/1913353‑1913625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`