BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,204,490	(A)_7→8	pseudogene (175/3632 nt)	CXP41_RS11705 →	DUF4132 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,204,483	1	.	A	94.6%	1837.7 / 80.3	498	pseudogene (168/3632 nt)	CXP41_RS11705	DUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand): ref base . (8/19); new base A (239/230); total (248/250)
Fisher's exact test for biased strand distribution p-value = 4.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 531 total aligned reads displayed.

AACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑A‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCGCG  >  NZ_CP025268/2204346‑2204617

aaCGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAgaag‑a‑a                                                                                                                                          <  1:3586837/140‑1 (MQ=255)

   gCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑a‑aaa‑a                                                                                                                                      <  1:7785454/140‑1 (MQ=255)

    cccAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑a‑aaa‑a‑a                                                                                                                                    >  1:4131944/1‑140 (MQ=255)

       aaCTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑a‑aaa‑a‑a‑aac                                                                                                                                >  1:4571557/1‑138 (MQ=255)

        aCTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑a‑aaa‑a‑a‑aaca                                                                                                                               <  1:3420788/140‑4 (MQ=255)

        aCTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGAGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTTCTACAAAAATCTGAGAAG‑a‑aaa‑a‑a‑aaca                                                                                                                               <  2:3583989/140‑4 (MQ=255)

            gAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGt                                                                                                                           >  1:10931147/1‑140 (MQ=255)

            gAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑A‑AAA‑A‑A‑ATCATTGt                                                                                                                           >  1:670659/1‑140 (MQ=255)

                  aaaTATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCg                                                                                                                     >  2:3340466/1‑140 (MQ=255)

                      ataAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTAt                                                                                                                 >  2:2364096/1‑140 (MQ=255)

                       taAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATg                                                                                                                >  2:11262192/1‑140 (MQ=255)

                            aaGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAAt                                                                                                           >  1:7733825/1‑140 (MQ=255)

                                   aaCGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCAc                                                                                                    <  1:4011176/140‑1 (MQ=255)

                                   aaCGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCAc                                                                                                    <  2:7172472/140‑1 (MQ=255)

                                   aaCGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCAc                                                                                                    <  1:5378608/140‑1 (MQ=255)

                                         gcTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGt                                                                                              >  1:4426219/1‑140 (MQ=255)

                                           tCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTgg                                                                                            <  1:7950660/140‑1 (MQ=255)

                                             aGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCa                                                                                          >  1:7384188/1‑140 (MQ=255)

                                                cATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                                       <  1:7317711/140‑1 (MQ=255)

                                                cATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                                       <  1:8934266/140‑1 (MQ=255)

                                                    gtcgCTGGCCGGGCGAACCAGTGTCCGTTATAAATGGAAGTCTCATCCAGACATTTGTTGTTGAATTGCTACAAAAAGCTGAGATG‑A‑AAA‑ATA‑A‑CATTGTCTGGCGTTAGGAGACTTGTGCACTGGAGTGGCAGTcaggc                                                                                   >  2:7769453/1‑136 (MQ=255)

                                                       gCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATc                                                                                >  1:10804340/1‑140 (MQ=255)

                                                          cgCCGTGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTAAAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CAGTGTCTGGCGTTATGAGAATATTTCACTGGAGTGGCAGTCCGACATCACg                                                                             <  2:616923/139‑1 (MQ=255)

                                                           gCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGc                                                                            >  1:3307164/1‑140 (MQ=255)

                                                           gCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGc                                                                            <  2:4582555/140‑1 (MQ=255)

                                                            ccGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCa                                                                           <  1:2863677/140‑1 (MQ=255)

                                                                gCGAACCCGTGTCCGTTATCACTGGAAGTCTCATCCAGACCTTGGGTGATGAATTGCTACAAAAAGCTGAGTAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTAGGAGAATGTGTCTCTGGAGTGGCAGTCCGCCATCACGCAGGcg                                                                       >  2:727268/1‑139 (MQ=255)

                                                                gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGATTTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGACTTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                       >  1:4845066/1‑140 (MQ=255)

                                                                gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                       >  2:1078671/1‑140 (MQ=255)

                                                                gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                       >  2:5415540/1‑140 (MQ=255)

                                                                gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                       <  1:6816676/140‑1 (MQ=255)

                                                                     ccAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGCGTGGCAGTCCGCCATCACGCAGGCCATCaa                                                                  <  2:4936791/140‑1 (MQ=255)

                                                                          gtCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGa                                                             <  1:5511308/140‑1 (MQ=255)

                                                                             cGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCg                                                          <  1:6317452/140‑1 (MQ=255)

                                                                             cGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTAATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCg                                                          >  2:5065765/1‑140 (MQ=255)

                                                                              gTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCgg                                                         >  2:10647530/1‑140 (MQ=255)

                                                                               ttATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGc                                                        <  1:8720370/140‑1 (MQ=255)

                                                                               ttATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGc                                                        >  1:11205567/1‑140 (MQ=255)

                                                                               ttATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGc                                                        <  1:7526750/140‑1 (MQ=255)

                                                                                tATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCg                                                       >  2:1372763/1‑140 (MQ=255)

                                                                                tATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCAACAAACAGCTGAGAAG‑ACAAA‑A‑A‑A‑CAGTGTCTGGCGTTATGAGAATGTTTCACTGGCGGGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCg                                                       <  2:2044552/140‑1 (MQ=255)

                                                                                  tCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGaa                                                     <  2:5227462/140‑1 (MQ=255)

                                                                                   cACTGGAAGTCTCATCCAGACATTGGGTGATGGATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAac                                                    >  1:9470138/1‑140 (MQ=255)

                                                                                   cACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAac                                                    >  2:4095851/1‑140 (MQ=255)

                                                                                   cACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAac                                                    >  2:4442366/1‑140 (MQ=255)

                                                                                      tGGAAGTCACATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAAcaca                                                 <  2:871216/140‑1 (MQ=255)

                                                                                       ggAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACaa                                                <  2:2568013/140‑1 (MQ=255)

                                                                                       ggAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTATCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAAcacat                                                >  2:5872826/1‑139 (MQ=255)

                                                                                          aGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAAcc                                             <  1:2428938/140‑1 (MQ=255)

                                                                                            tctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCt                                           <  1:1029659/140‑1 (MQ=255)

                                                                                            tctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCt                                           >  1:9986429/1‑140 (MQ=255)

                                                                                            tctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCt                                           >  1:11482402/1‑140 (MQ=255)

                                                                                             ctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTc                                          >  1:8798867/1‑140 (MQ=255)

                                                                                              tcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCa                                         >  2:4178363/1‑140 (MQ=255)

                                                                                              tcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCa                                         >  2:6422525/1‑140 (MQ=255)

                                                                                                aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTACGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAAt                                       >  1:2683284/1‑140 (MQ=255)

                                                                                                aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGAAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACACACCCTCAAt                                       >  2:8289814/1‑140 (MQ=255)

                                                                                                 tCCTGACATTGGGTTATGAATTGATACAAAAAGATGATAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc                                      <  2:8472401/140‑1 (MQ=255)

                                                                                                 tCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAAta                                      <  2:4765411/140‑2 (MQ=255)

                                                                                                  ccAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcc                                     >  1:647965/1‑140 (MQ=255)

                                                                                                      aCATTGGGTGATGAATTGCTACAAAAATCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggc                                 <  1:4327228/140‑1 (MQ=255)

                                                                                                        aTTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggccc                               >  1:829050/1‑140 (MQ=255)

                                                                                                         ttGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggcccg                              <  2:9109517/140‑1 (MQ=255)

                                                                                                          tGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGTAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggcccgg                             >  2:4535637/1‑140 (MQ=255)

                                                                                                          tGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggcccgg                             >  1:1348912/1‑140 (MQ=255)

                                                                                                          tGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggcccgg                             >  1:2200713/1‑140 (MQ=255)

                                                                                                          tGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggcccgg                             >  1:4995209/1‑140 (MQ=255)

                                                                                                          tGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggcccgg                             >  1:9269699/1‑140 (MQ=255)

                                                                                                          tGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCAACAACTTGATAGGCGAACACAAACCCTCAATcccggcccgg                             >  2:11475437/1‑140 (MQ=255)

                                                                                                            ggTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGAc                           <  1:1542661/140‑1 (MQ=255)

                                                                                                                atgaATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATg                       >  2:8925669/1‑140 (MQ=255)

                                                                                                                atgaATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATg                       >  1:10229146/1‑140 (MQ=255)

                                                                                                                atgaATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATg                       >  2:158709/1‑140 (MQ=255)

                                                                                                                 tgaATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATgg                      <  2:2672698/140‑1 (MQ=255)

                                                                                                                 tgaATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATgg                      <  1:9824540/140‑1 (MQ=255)

                                                                                                                 tgaATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATgg                      <  1:1719463/140‑1 (MQ=255)

                                                                                                                 tgaATTGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATgg                      <  2:6680485/140‑1 (MQ=255)

                                                                                                                      tGCTACCAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCTGTCCGCCATCACGCAGGCCTTCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:9350622/140‑1 (MQ=255)

                                                                                                                      tGCTACAAAAATCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:5767504/140‑1 (MQ=255)

                                                                                                                      tGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CCTTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:10559878/140‑1 (MQ=255)

                                                                                                                      tGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:10716026/140‑1 (MQ=255)

                                                                                                                      tGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:3045671/140‑1 (MQ=255)

                                                                                                                      tGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:8126626/140‑1 (MQ=255)

                                                                                                                      tGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:5825609/140‑1 (MQ=255)

                                                                                                                      tGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:6039295/140‑1 (MQ=255)

                                                                                                                      tGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:694109/140‑1 (MQ=255)

                                                                                                                      tGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:8528212/140‑1 (MQ=255)

                                                                                                                      tGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:10821710/140‑1 (MQ=255)

                                                                                                                      tGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:3748254/140‑1 (MQ=255)

                                                                                                                      tGCTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTCATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:2031620/140‑1 (MQ=255)

                                                                                                                      tGCTACAAAAAGCTGAGAAG‑A‑AAA‑A‑ATA‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:9551923/140‑1 (MQ=255)

                                                                                                                      tGCTACAAAAAGCTCAGAAG‑A‑AAA‑ACA‑A‑CCTTGTCTGGCGTTATGAGAATATTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTGGATCGGCGAACACATACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:10250700/140‑1 (MQ=255)

                                                                                                                      tGCTACAAAAAGCGGACAAG‑A‑AAACA‑A‑A‑CATTGTCTGGCGTTATGAGAAGTTGTCACGGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGTTCGGCGAACACACACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:11016340/140‑1 (MQ=255)

                                                                                                                      tGCAACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTTTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCTCCATCACGCAGGCCATCAACTTGATCGTCGAACACAAACCCACAATCCCGGCCCGGACAATGgcggcg                 <  2:7395654/140‑1 (MQ=255)

                                                                                                                       gCTACAAAAAGCTGAGAAGCA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTATTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGgcgc                <  2:8816622/140‑1 (MQ=255)

                                                                                                                        cTACAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCt               <  2:2960197/140‑1 (MQ=255)

                                                                                                                           cAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGc            <  1:3904051/140‑1 (MQ=255)

                                                                                                                           cAAAAAGCTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGc            >  2:5678531/1‑140 (MQ=255)

                                                                                                                                cgCTTAGAAG‑A‑AAATA‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTACACTGGAGTGGCAGTCCGCCATCACGCAGGACATCAACTTGATCGGCGAACACAAACCCTCAATCACGGACCTGACAATGCCGGCGCTAGCCTGTa       <  2:7581248/139‑1 (MQ=255)

                                                                                                                                  cTGAGAAGAA‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATc     >  1:1004352/1‑140 (MQ=255)

AACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑A‑AAA‑A‑A‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCGCG  >  NZ_CP025268/2204346‑2204617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`