BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,769,852	+C	intergenic (‑52/‑89)	yfjM ← / → rnlA	DUF1524 domain‑containing protein/type II toxin‑antitoxin system toxin mRNA endoribonuclease RnlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,769,848	1	.	C	94.9%	1756.3 / 80.1	536	intergenic (‑48/‑93)	yfjM/rnlA	DUF1524 domain‑containing protein/type II toxin‑antitoxin system toxin mRNA endoribonuclease RnlA
Reads supporting (aligned to +/- strand): ref base . (7/20); new base C (230/276); total (238/298)
Fisher's exact test for biased strand distribution p-value = 4.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 563 total aligned reads displayed.

TCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TG‑C‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAAT  >  NZ_CP025268/2769710‑2769985

   tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TG‑c‑c‑c‑c                                                                                                                                       <  1:2124951/140‑1 (MQ=255)

   tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TG‑c‑c‑c‑c                                                                                                                                       <  2:5495017/140‑1 (MQ=255)

       tgctgGCGAATAATTACTAAGTCTCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGGTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TG‑c‑c‑c‑cctat                                                                                                                                   <  1:8082475/140‑5 (MQ=255)

       tgctgGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TG‑c‑c‑c‑cctat                                                                                                                                   <  1:2275712/140‑5 (MQ=255)

            gCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAgg                                                                                                                               <  1:5137530/140‑1 (MQ=255)

                   atTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTaa                                                                                                                        >  2:5903897/1‑140 (MQ=255)

                      aCTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAAtt                                                                                                                     >  2:9171360/1‑140 (MQ=255)

                         aaGTATCGCGGTGCTTCTTGGTGCGATCAGGCAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGGGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAg                                                                                                                  <  2:4086148/140‑1 (MQ=255)

                         aaGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAg                                                                                                                  <  1:6171307/140‑1 (MQ=255)

                         aaGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAg                                                                                                                  <  2:5684607/140‑1 (MQ=255)

                         aaGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAg                                                                                                                  <  1:10587607/140‑1 (MQ=255)

                           gTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCa                                                                                                                <  1:8397828/140‑1 (MQ=255)

                           gTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGGTCCCGAAACGATGGACATCATCCCCCATTTTGTTGACTGCCCACCCGGAAGACGATCCTGACCTAGAGTTGGAGTTA‑C‑C‑C‑CTATTCAGGTGGCTAAATTTAGCa                                                                                                                <  2:6942207/140‑1 (MQ=255)

                              tCGAGTTGCTGCTTGGTGAGATCCGGAAGAGGTTACCGAAACGATGGACATCATCCCCCCTTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAAt                                                                                                             <  1:2007300/140‑1 (MQ=255)

                              tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTGTTCAGGTGGCTAAATTTAGCAAAt                                                                                                             >  1:11082957/1‑140 (MQ=255)

                              tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAAt                                                                                                             >  2:7478393/1‑140 (MQ=255)

                              tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAAt                                                                                                             <  1:2492717/140‑1 (MQ=255)

                              tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGGTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAAt                                                                                                             >  2:3337431/1‑140 (MQ=255)

                               cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTGTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTGGGAG‑TGCC‑C‑C‑CTATGCAGGTGGCTAAATTTAGCAAATc                                                                                                            >  1:6274595/1‑140 (MQ=255)

                                   ttGCTGCTTGGTGAGATCAGGACGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                        <  1:7231311/140‑1 (MQ=255)

                                   ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                        <  1:11243563/140‑1 (MQ=255)

                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTCTAGGTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                                     >  2:5090708/1‑140 (MQ=255)

                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTCTTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                                     >  2:11105172/1‑140 (MQ=255)

                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                                     >  1:1611752/1‑140 (MQ=255)

                                          ttGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCa                                                                                                 <  1:8698763/140‑1 (MQ=255)

                                             gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                              <  1:1158793/140‑1 (MQ=255)

                                             gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                              <  1:5269350/140‑1 (MQ=255)

                                                agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                           <  1:10430571/140‑1 (MQ=255)

                                                  aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                         <  1:9071246/140‑1 (MQ=255)

                                                  aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                         <  1:5560427/140‑1 (MQ=255)

                                                  aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                         <  2:3234470/140‑1 (MQ=255)

                                                      ggACGAGGTTCCCGAAACGATGGACATCCTCCCCCATATTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCGAGCGTTGGAG‑TGCC‑C‑C‑CTAGGCAGGGGGCTCACTTTAGCAAATCAATACACTTCAGGGGGGtattat                                                                                     <  2:2083273/140‑1 (MQ=255)

                                                       gAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTattatt                                                                                    >  1:10045184/1‑140 (MQ=255)

                                                          gtggTTACCGATTCGATGGTCATCTTCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCATTTCAATACACTTCTGGGGGGTATTATTGTa                                                                                 <  1:3087500/138‑1 (MQ=255)

                                                          gagGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTa                                                                                 <  1:8835257/140‑1 (MQ=255)

                                                          gagGTTACAGAAACGATGGACATCATCCCCCATTTTGTGGATTTCCCCCCCGGTAGACGTTCCTGCCCTATCGTTGGAT‑TG‑C‑C‑CACTATTCATGTGGCTAAATTTATCAAATCATTACACTTCAGGGGGGTATTATTGTa                                                                                 <  2:5257410/140‑1 (MQ=255)

                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                                                               >  1:9728248/1‑140 (MQ=255)

                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGGATTATTGTaga                                                                               >  1:686324/1‑140 (MQ=255)

                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATGTAGCAAATCAATACACTTCTGGGGGGTATTATTGTaga                                                                               >  2:372935/1‑140 (MQ=255)

                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CGATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                                                               >  1:5479345/1‑140 (MQ=255)

                                                              ttACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGt                                                                             <  1:1405500/140‑1 (MQ=255)

                                                                aCCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCTCGGTAGACGATCCTGCACTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTACATTTAGCAAATCAATACACTTCAGGGGGGTTTTATTGTAGAGttt                                                                           <  2:2713207/140‑1 (MQ=255)

                                                                aCCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGttt                                                                           >  2:6083866/1‑140 (MQ=255)

                                                                  cGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                                                                         <  2:10282555/140‑1 (MQ=255)

                                                                     aaCGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTAGTGTAGAGTTTCCCCa                                                                      >  2:13525/1‑140 (MQ=255)

                                                                     aaCGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCAGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAAGTTAGCAAATCAATACACTTCAGGGGGTTATTATGGTAGAGTTTCCCCa                                                                      <  2:7157609/140‑1 (MQ=255)

                                                                     aaCGAGGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATGTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCa                                                                      >  2:8190896/1‑140 (MQ=255)

                                                                      aCGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCat                                                                     >  2:10379317/1‑140 (MQ=255)

                                                                      aCGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCat                                                                     <  2:4209878/140‑1 (MQ=255)

                                                                       cGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCata                                                                    <  1:4373121/140‑1 (MQ=255)

                                                                        gATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTTGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCatat                                                                   >  1:2786626/1‑140 (MQ=255)

                                                                           ggACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTCTTGTAGAGTTTCCCCATATGtt                                                                >  1:7450879/1‑140 (MQ=255)

                                                                               atcatcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTa                                                            >  1:2676884/1‑140 (MQ=255)

                                                                                 catcCCCCATTTTGTGGACTGCCCACCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TG‑C‑CAC‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGTGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg                                                          <  2:10140211/140‑1 (MQ=255)

                                                                                 catcCCCCACTTTGCGGACTGCCCCCCCGGTCGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTAGTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg                                                          <  1:7683559/140‑1 (MQ=255)

                                                                                    ccaccATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TG‑CAC‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGa                                                       <  2:7520181/137‑1 (MQ=255)

                                                                                      cccATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                                                                         >  2:4823302/1‑120 (MQ=255)

                                                                                               tgGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCATGTGGCTAAATTTAGCCAATCAATACACTTCAGGGGGGTATTATTGGAGAGTTTCCCCTTATGTTTCTCTGGGATCCAGGACATg                                            <  2:4505442/140‑1 (MQ=255)

                                                                                               tgGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATg                                            <  1:1846171/140‑1 (MQ=255)

                                                                                                    tGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACact                                       >  2:1141004/1‑138 (MQ=255)

                                                                                                    tGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAAt                                       >  2:6433538/1‑140 (MQ=255)

                                                                                                    tGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCAGATGTTTCTATGGGATCCAGGAAATGACAAt                                       >  2:5150265/1‑140 (MQ=255)

                                                                                                      cccccccGTTAGACTATCCTGCCCTATATGTGGAG‑TG‑C‑C‑CACTATTCATGTGTCTAAATTTAGCAAATCAATACACTTCAGGGTGGTATTATTGTAGAGTTTCCCCATATGTTACTATGTGATCCAGGAAATGACAATCa                                     <  2:10896726/140‑1 (MQ=255)

                                                                                                      cccccccGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCa                                     >  2:8036147/1‑140 (MQ=255)

                                                                                                      cccccccGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCa                                     >  1:10278990/1‑140 (MQ=255)

                                                                                                         ccccGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATc                                      >  1:3956467/1‑136 (MQ=255)

                                                                                                         ccccGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATc                                      <  2:3956467/136‑1 (MQ=255)

                                                                                                         ccccGGTAGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGa                                  >  1:8253226/1‑140 (MQ=255)

                                                                                                                aGACGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                           <  1:7792294/140‑1 (MQ=255)

                                                                                                                aGACGATCCTGCCCTAGAGTTGGAG‑TGCC‑C‑C‑CTATTCCTGTGGCTAAAATGAGAAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                           <  2:780471/140‑1 (MQ=255)

                                                                                                                   cGATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaaaaa                        <  2:2390314/140‑1 (MQ=255)

                                                                                                                    gATCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAAc                       >  2:10638515/1‑140 (MQ=255)

                                                                                                                     ctCCTGCCCTAGAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACATTCAGGAGTTACAAAAACt                      <  2:4293138/139‑1 (MQ=255)

                                                                                                                     aTCCTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACt                      >  1:9188659/1‑140 (MQ=255)

                                                                                                                       ccTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTa                    >  2:475203/1‑140 (MQ=255)

                                                                                                                       ccTGCCCTATAGTTGGAG‑TGCC‑C‑C‑CGATTCAGGTGGCTAAATATAGCAAATCAATACCCGTCAGGGGGGTAGTCTGGTAGAGTTGCCCCATATGTTGCTATGGGAGCAAGGAAATGTGCATCAGTAGTTTCTCAAACtgg                    >  2:11255915/1‑138 (MQ=255)

                                                                                                                         tGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTaaa                  >  2:2533582/1‑140 (MQ=255)

                                                                                                                         tGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTaaa                  >  1:1485707/1‑140 (MQ=255)

                                                                                                                         tGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTaaa                  >  2:6849879/1‑140 (MQ=255)

                                                                                                                         tGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTaaa                  <  1:9355858/140‑1 (MQ=255)

                                                                                                                         tGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTaaa                  >  2:1903466/1‑140 (MQ=255)

                                                                                                                         tGCCCTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTaaa                  >  2:1746055/1‑140 (MQ=255)

                                                                                                                           cccTATAGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATc                >  1:4566263/1‑140 (MQ=255)

                                                                                                                                taGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTTGCTATAGTGAGCAAATCTATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTACTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAAACTGGTc           <  2:8368378/140‑1 (MQ=255)

                                                                                                                                taGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTCTTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc           >  2:9405203/1‑140 (MQ=255)

                                                                                                                                taGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAACTGACAATCAGGAGTTACAAAAACTTAAATCTGGTc           >  2:6208538/1‑140 (MQ=255)

                                                                                                                                taGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                           <  1:3462106/124‑1 (MQ=255)

                                                                                                                                taGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                           >  2:3462106/1‑124 (MQ=255)

                                                                                                                                taGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc           <  1:4988487/140‑1 (MQ=255)

                                                                                                                                taGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc           >  2:9770143/1‑140 (MQ=255)

                                                                                                                                taGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc           >  1:3605806/1‑140 (MQ=255)

                                                                                                                                taGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc           <  1:3362310/140‑1 (MQ=255)

                                                                                                                                taGTTGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc           >  1:1736018/1‑140 (MQ=255)

                                                                                                                                    tGGAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAggg       <  2:1004010/140‑1 (MQ=255)

                                                                                                                                     ggAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc      >  2:9885726/1‑140 (MQ=255)

                                                                                                                                     ggAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc      >  2:3026278/1‑140 (MQ=255)

                                                                                                                                     ggAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc      >  1:11203115/1‑140 (MQ=255)

                                                                                                                                     ggAG‑TGCC‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc      >  1:5926026/1‑140 (MQ=255)

                                                                                                                                      gAG‑TGCC‑C‑C‑CTATTCAGGTGTATAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTTTAGAGTTTCCCCTTATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCa     <  1:9882316/140‑1 (MQ=255)

                                                                                                                                      gAG‑TG‑CAC‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCa     <  2:8866556/140‑1 (MQ=255)

TCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAG‑TG‑C‑C‑C‑CTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAAT  >  NZ_CP025268/2769710‑2769985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`