BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,426,468	+C	pseudogene (249/275 nt)	yjfY ←	DUF1471 family protein YjfY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,426,468	1	.	C	98.7%	1852.3 / 12.1	539	pseudogene (249/275 nt)	yjfY	DUF1471 family protein YjfY
Reads supporting (aligned to +/- strand): ref base . (1/6); new base C (239/292); total (240/299)
Fisher's exact test for biased strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 555 total aligned reads displayed.

ATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGC‑G‑TG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGC  >  NZ_CP025268/4426329‑4426601

aTTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑Tg                                                                                                                                        <  1:2734746/140‑1 (MQ=255)

          tCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCg                                                                                                                              <  1:6188494/140‑1 (MQ=255)

          tCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCg                                                                                                                              <  1:1663545/140‑1 (MQ=255)

           cGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGt                                                                                                                             <  1:2073723/140‑1 (MQ=255)

               agaTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACt                                                                                                                         <  2:8008835/140‑1 (MQ=255)

                   aaCTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTcccc                                                                                                                     >  1:3992143/1‑140 (MQ=255)

                    acttgacTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTCTGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCg                                                                                                                    <  2:9060604/134‑1 (MQ=255)

                    aCTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCg                                                                                                                    >  1:6939725/1‑140 (MQ=255)

                    aCTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCg                                                                                                                    <  1:238318/140‑1 (MQ=255)

                     cTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCgg                                                                                                                   >  1:9595516/1‑140 (MQ=255)

                        gTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTCGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTc                                                                                                                >  1:7363044/1‑140 (MQ=255)

                        gTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTc                                                                                                                >  2:10840537/1‑140 (MQ=255)

                         tCTAATTCCGCAGCCGTTTTTCAAAAACAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTcc                                                                                                               <  1:865296/140‑1 (MQ=255)

                         tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTcc                                                                                                               <  1:1242010/140‑1 (MQ=255)

                         tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTcc                                                                                                               <  2:4976862/140‑1 (MQ=255)

                         tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTcc                                                                                                               <  1:7599429/140‑1 (MQ=255)

                         tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTcc                                                                                                               <  1:5654372/140‑1 (MQ=255)

                         tCTAAGTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTcc                                                                                                               <  1:10480258/140‑1 (MQ=255)

                           tAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCg                                                                                                             >  1:10292665/1‑140 (MQ=255)

                                 cGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATTTCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAg                                                                                                       <  2:8473175/140‑1 (MQ=255)

                                   cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCACACTATGGCAATGTTTCGTTGGTTATCCCTGCTGCAAGCGAGAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                                                     <  1:3385150/140‑1 (MQ=255)

                                     gCCGTAATTCAAACAATCGCCCCCAGCGAAGGGGGCAAAGCCACCTATCGCAATGTGTCGTTGGTAATACCAGGAGCTAGCGATACATATCCGCGCTTCCTT‑TC‑ATATTGCCGTTACTCCCCGGGAACCGCATCAGAAtt                                                                                                   <  2:11224411/140‑1 (MQ=255)

                                      ccGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  <  2:3069492/140‑1 (MQ=255)

                                      ccGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  <  2:4664663/140‑1 (MQ=255)

                                      ccGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  <  2:9358871/140‑1 (MQ=255)

                                      ccGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  <  2:10515286/140‑1 (MQ=255)

                                      ccGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGGTCCCGCATCAGAATTa                                                                                                  <  2:2630790/140‑1 (MQ=255)

                                        gattttCAAAAAAAAGCCCCCTGCGACGGGGGCAAAGCCAACTATGGCAATGTTTCGTTGGTTATACCTGGAGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:7178788/138‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGNGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:6096942/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCACACTAGGGCAATGTTTCGTTGGTTAGACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:1005481/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAACCTCTGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:11095147/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCTTTACTCCCCGGTTCCCGCATCAGAATTAct                                                                                                <  2:1157059/140‑2 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:5367400/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:5557429/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:5811714/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:5045293/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:4213439/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:360678/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:9216131/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTAGACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:3810121/140‑1 (MQ=255)

                                          ttttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTg                                                                                              <  2:2079258/140‑1 (MQ=255)

                                              cAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGgtag                                                                                          <  2:3463414/140‑1 (MQ=255)

                                               aaaaaaaaGCCCCCTGCGAAGGGGGCAAATACAACTATGTCAATGTTTCGTTGGTTATACCTGGTGCTAGAGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGgtagt                                                                                         <  2:2777492/140‑1 (MQ=255)

                                                aaaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGgtagta                                                                                        >  2:1934330/1‑140 (MQ=255)

                                                   aaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGt                                                                                     <  1:11320845/140‑1 (MQ=255)

                                                       gCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTgcg                                                                                 <  1:2277135/140‑1 (MQ=255)

                                                         ccccTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATCCCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGcc                                                                               <  1:5301998/140‑1 (MQ=255)

                                                                  aGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATc                                                                      <  2:10289706/140‑1 (MQ=255)

                                                                    ggggCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTg                                                                    >  2:6992628/1‑140 (MQ=255)

                                                                      ggCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGtt                                                                  >  2:3150329/1‑140 (MQ=255)

                                                                      ggCAAAGCAAACCCTGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑GTTC‑ATATTGCCGTTACTACCCGGTTCCCGCATCATAATTACGTGGTAGTACGTTGCGCCTTGCGCATcattt                                                                  <  2:1549552/140‑5 (MQ=255)

                                                                       gcaaagcaaaCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGttt                                                                 >  1:879636/1‑140 (MQ=255)

                                                                               aaCTCTGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCAttt                                                         <  1:5824248/140‑1 (MQ=255)

                                                                               aaCTAGGGCACTGTTTCGTTGGTGAGACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCAttt                                                         <  2:6103019/140‑1 (MQ=255)

                                                                                    tGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGc                                                    >  1:4717504/1‑140 (MQ=255)

                                                                                      gCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCt                                                  >  2:8145705/1‑140 (MQ=255)

                                                                                          tGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACg                                              >  2:5299455/1‑140 (MQ=255)

                                                                                          tGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACg                                              >  1:7091726/1‑140 (MQ=255)

                                                                                          tGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTAAGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACg                                              >  2:5896428/1‑140 (MQ=255)

                                                                                           gTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGt                                             <  1:1088312/140‑1 (MQ=255)

                                                                                           gTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGt                                             >  2:2227505/1‑140 (MQ=255)

                                                                                            tttCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTg                                            >  2:5482456/1‑140 (MQ=255)

                                                                                            tttCGTTGGTTATACCTGGAGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTg                                            >  1:4534177/1‑140 (MQ=255)

                                                                                              tCGTTGGTTATACCTGGTGCTAGCGATAAATCTCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCt                                          <  2:7523362/140‑1 (MQ=255)

                                                                                              tCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCt                                          <  1:4913230/140‑1 (MQ=255)

                                                                                              tCGTTGGTTATACCTCGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCt                                          <  1:9497884/140‑1 (MQ=255)

                                                                                                      tataCCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATCTTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCGTGCGCATCTGTGTCTTCATTTAATGCCTGACGTGCTTCGCTTTc                                  >  2:8351958/1‑140 (MQ=255)

                                                                                                      tataCCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTc                                  <  1:3158817/140‑1 (MQ=255)

                                                                                                        taCCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGACTGACGTGCTTCGCTTTCAg                                >  2:3401783/1‑140 (MQ=255)

                                                                                                        taCCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATAGTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAg                                <  2:4532282/140‑1 (MQ=255)

                                                                                                         aCCTGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGt                               >  1:10580290/1‑140 (MQ=255)

                                                                                                            tGGTGCTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTTCTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCTTTTAATGCCTGACGTGCTTCGCTTTCAGTGGc                            <  2:654425/140‑1 (MQ=255)

                                                                                                              gtgcTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  1:6768575/1‑140 (MQ=255)

                                                                                                              gtgcTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  2:9495686/1‑140 (MQ=255)

                                                                                                              gtgcTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          <  2:2454180/140‑1 (MQ=255)

                                                                                                              gtgcTAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  1:2956816/1‑140 (MQ=255)

                                                                                                               tgctAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGaa                         >  2:8914759/4‑140 (MQ=255)

                                                                                                               tgctAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGaa                         >  1:5138645/4‑140 (MQ=255)

                                                                                                                  tAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAAtt                      >  1:6563095/1‑140 (MQ=255)

                                                                                                                  tAGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGGTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAAtt                      <  2:5635646/140‑1 (MQ=255)

                                                                                                                   aGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCCTTTAATGCCTGACGTGCTTCGCTGTCAGTGGCGAAATTa                     >  1:10032333/1‑140 (MQ=255)

                                                                                                                   aGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTa                     >  2:7708555/1‑140 (MQ=255)

                                                                                                                   aGCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTa                     >  2:4009261/1‑140 (MQ=255)

                                                                                                                    gCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAt                    >  1:3274924/1‑140 (MQ=255)

                                                                                                                    gCGATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAt                    >  2:3315599/1‑140 (MQ=255)

                                                                                                                      gATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtga                  <  2:1342406/140‑1 (MQ=255)

                                                                                                                      gATAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGGGGTAGTACGTTGCGCCTAGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtga                  <  1:9126644/140‑1 (MQ=255)

                                                                                                                       aTAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  1:788658/140‑1 (MQ=255)

                                                                                                                       aTAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  1:6992328/140‑1 (MQ=255)

                                                                                                                       aTAAATATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCAGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  2:9780142/140‑1 (MQ=255)

                                                                                                                         aaaTATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCCTCTGGTGCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgattg               >  2:1782128/1‑140 (MQ=255)

                                                                                                                         aaaTATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgattg               >  2:6769513/1‑140 (MQ=255)

                                                                                                                         aaaTATCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgattg               >  1:4143211/1‑140 (MQ=255)

                                                                                                                              tCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGAtata          >  2:6250959/1‑140 (MQ=255)

                                                                                                                              tCCGCGCTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGAtata          >  1:1538687/1‑140 (MQ=255)

                                                                                                                                 gcgcTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt       >  1:2530410/1‑140 (MQ=255)

                                                                                                                                 gcgcTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt       <  1:5076517/140‑1 (MQ=255)

                                                                                                                                  cgcTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCCTCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATc      >  2:9596975/1‑140 (MQ=255)

                                                                                                                                  cgcTGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATc      >  2:2842764/1‑140 (MQ=255)

                                                                                                                                  cgcAGGC‑G‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCAGTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGGAAGTATGTTTGATATACATc      >  1:8262869/1‑140 (MQ=255)

ATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGC‑G‑TG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGC  >  NZ_CP025268/4426329‑4426601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`