BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,542,898	(A)_6→7	pseudogene (27/602 nt)	fimB →	type 1 fimbria switch DNA invertase FimB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,542,892	1	.	A	99.1%	4771.9 / 20.7	1228	pseudogene (21/602 nt)	fimB	type 1 fimbria switch DNA invertase FimB
Reads supporting (aligned to +/- strand): ref base . (7/4); new base A (763/451); total (771/457)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1349 total aligned reads displayed.

GTCCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑A‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGT  >  NZ_CP025268/4542752‑4543026

    aCTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑a‑aa                                                                                                                                      <  1:7854372/140‑1 (MQ=255)

          gttagttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑a‑aaa‑aaagg                                                                                                                               >  2:5705829/1‑137 (MQ=255)

          gttagttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑a‑aaa‑aaagg                                                                                                                               <  2:6022812/140‑4 (MQ=255)

          gttagttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑ACAAA‑AAgg                                                                                                                                >  2:10227290/1‑140 (MQ=255)

           ttagttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑a‑aaa‑aaagga                                                                                                                              <  2:9816902/140‑5 (MQ=255)

               ttattaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTCGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACtt                                                                                                                           >  2:8675920/1‑140 (MQ=255)

               ttattaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACtt                                                                                                                           >  2:6798567/1‑140 (MQ=255)

               ttattaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACta                                                                                                                           >  2:345471/1‑139 (MQ=255)

                 attaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTca                                                                                                                         >  2:7674063/1‑139 (MQ=255)

                  ttaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCTCAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCt                                                                                                                        <  1:9013906/140‑1 (MQ=255)

                  ttaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCt                                                                                                                        >  2:1056764/1‑140 (MQ=255)

                  ttaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCt                                                                                                                        >  2:9095690/1‑140 (MQ=255)

                  ttaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCt                                                                                                                        <  1:1167883/140‑1 (MQ=255)

                  ttaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCt                                                                                                                        <  2:10930342/140‑1 (MQ=255)

                  ttaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCt                                                                                                                        <  1:5895639/140‑1 (MQ=255)

                  ttaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCt                                                                                                                        <  1:3257664/140‑1 (MQ=255)

                   taAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTg                                                                                                                       >  1:3784122/1‑140 (MQ=255)

                    aaGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGa                                                                                                                      >  1:3910209/1‑140 (MQ=255)

                      gCAAACGTTCGATTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGAcc                                                                                                                    <  1:6298410/140‑1 (MQ=255)

                         aaCGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCt                                                                                                                        <  2:3322738/133‑1 (MQ=255)

                          aCGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATa                                                                                                                <  2:9284819/140‑1 (MQ=255)

                               cGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGaa                                                                                                           >  1:1074793/1‑140 (MQ=255)

                               cGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGaa                                                                                                           >  2:1000491/1‑140 (MQ=255)

                               cGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGaa                                                                                                           >  1:2721958/1‑140 (MQ=255)

                                   tttATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCg                                                                                                       <  1:2225818/140‑1 (MQ=255)

                                        aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  1:5419586/140‑1 (MQ=255)

                                        aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  1:2402184/140‑1 (MQ=255)

                                        aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  1:2555391/140‑1 (MQ=255)

                                            aTAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTTTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTcc                                                                                              >  2:10402157/1‑140 (MQ=255)

                                            aTAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGaa                                                                                                     >  1:9495503/1‑133 (MQ=255)

                                            aTAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGaa                                                                                                     <  2:9495502/133‑1 (MQ=255)

                                            aTAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  2:3764095/136‑1 (MQ=255)

                                            aTAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTcc                                                                                              >  1:6569906/1‑140 (MQ=255)

                                            aTAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTca                                                                                              >  2:8128732/1‑139 (MQ=255)

                                              aaTCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCtt                                                                                            >  2:3133357/1‑140 (MQ=255)

                                              aaTCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCtt                                                                                            >  2:5464758/1‑140 (MQ=255)

                                              aaTCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCtt                                                                                            >  1:10434284/1‑140 (MQ=255)

                                               aTCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTa                                                                                           <  1:8683159/140‑1 (MQ=255)

                                                 cAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTaaa                                                                                         <  1:7712480/140‑1 (MQ=255)

                                                 cAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTaaa                                                                                         <  1:7993738/140‑1 (MQ=255)

                                                  aGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAag                                                                                        <  2:3670167/140‑1 (MQ=255)

                                                    gATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTaaa                                                                                         >  1:1339063/1‑137 (MQ=255)

                                                    gATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAagca                                                                                      >  1:3618309/1‑140 (MQ=255)

                                                       aaaaaTGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAgcagca                                                                                   <  1:11246654/140‑1 (MQ=255)

                                                         aaaTGTTGGATTATTGCTAACCCAGCACAGCTCGTGCGCGTCTGTAATTATAAGGGAAAAACGATGATGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCaaa                                                                                 >  1:10017156/1‑140 (MQ=255)

                                                              ttGGATTATTGCTNACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCg                                                                            >  1:9853387/1‑140 (MQ=255)

                                                              ttGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCGGCAAATACCg                                                                            >  2:6624370/1‑140 (MQ=255)

                                                              ttGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCg                                                                            >  1:2021782/1‑140 (MQ=255)

                                                              ttGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCg                                                                            >  2:11264247/1‑140 (MQ=255)

                                                              ttGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCg                                                                            >  1:4997513/1‑140 (MQ=255)

                                                              ttGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCg                                                                            >  1:1615613/1‑140 (MQ=255)

                                                              ttGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCg                                                                            >  1:7117231/1‑140 (MQ=255)

                                                                          tAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcag                                                                <  1:6751541/140‑1 (MQ=255)

                                                                          tAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcag                                                                <  1:11400707/140‑1 (MQ=255)

                                                                          tAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcag                                                                <  2:2638319/140‑1 (MQ=255)

                                                                            aCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagca                                                              <  1:4808104/140‑1 (MQ=255)

                                                                            aCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagca                                                              <  2:9678990/140‑1 (MQ=255)

                                                                            aCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagca                                                              <  2:6133954/140‑1 (MQ=255)

                                                                             cccAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCAc                                                             >  2:5202388/1‑140 (MQ=255)

                                                                                aGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGACGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTa                                                          >  2:1574330/1‑140 (MQ=255)

                                                                                aGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCgg                                                                           <  2:6360483/123‑1 (MQ=255)

                                                                                aGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTa                                                          >  1:466193/1‑140 (MQ=255)

                                                                                aGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTa                                                          >  2:4541252/1‑140 (MQ=255)

                                                                                 gCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTaa                                                         >  1:1801768/1‑140 (MQ=255)

                                                                                 gCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTaa                                                         >  1:4094297/1‑140 (MQ=255)

                                                                                  cacaGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAat                                                        >  1:4306789/1‑140 (MQ=255)

                                                                                   acaGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagca                                                              >  1:5748589/1‑133 (MQ=255)

                                                                                       cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  1:3000839/1‑140 (MQ=255)

                                                                                       cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  2:7383404/1‑140 (MQ=255)

                                                                                       cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  2:5006002/1‑140 (MQ=255)

                                                                                         aGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTc                                                 <  2:8497845/140‑1 (MQ=255)

                                                                                         aGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTc                                                 >  1:8478190/1‑140 (MQ=255)

                                                                                          gTGCGCGTCTGTAATTATAAGGGAAAAACGATGCAGACTACGGCTGATAA‑C‑A‑AAACAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCt                                                <  2:7233314/140‑1 (MQ=255)

                                                                                          gTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCt                                                >  1:3408996/1‑140 (MQ=255)

                                                                                               cgTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActt                                           >  1:5174665/1‑140 (MQ=255)

                                                                                                 tCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTa                                                                                           <  2:4062637/90‑1 (MQ=255)

                                                                                                   tGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttgct                                       <  1:7547424/140‑1 (MQ=255)

                                                                                                     tAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttgcttt                                     >  1:610000/1‑140 (MQ=255)

                                                                                                            aaGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAat                                                        >  2:2475636/1‑114 (MQ=255)

                                                                                                             aGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTa                               <  2:4792780/138‑1 (MQ=255)

                                                                                                               ggAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCa                           <  1:9261912/140‑1 (MQ=255)

                                                                                                               ggAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCa                           >  1:11034566/1‑140 (MQ=255)

                                                                                                               ggAAAAACGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCa                           <  1:9674533/140‑1 (MQ=255)

                                                                                                                 aaaaaCGATGAAGAATAAGGCTGATAACC‑A‑AAA‑AAGGAACTTCCTGACCCATCGTGAAATCGAATCACTCCTTAAAGCATCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATg                         <  2:7032412/140‑1 (MQ=255)

                                                                                                                 aaaaaCGATGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATg                         >  2:2115131/1‑140 (MQ=255)

                                                                                                                         tGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCgg                 >  2:7926710/1‑140 (MQ=255)

                                                                                                                         tGAAGAATAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCgg                 >  2:4285382/1‑140 (MQ=255)

                                                                                                                          gaagaaTCAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCggg                <  2:10757975/140‑1 (MQ=255)

                                                                                                                              aaTAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAg            >  1:8319233/1‑140 (MQ=255)

                                                                                                                                tAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTg          >  2:191628/1‑140 (MQ=255)

                                                                                                                                tAAGGCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTg          >  1:10287543/1‑140 (MQ=255)

                                                                                                                                   ggCTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGaaa       >  1:10540073/1‑140 (MQ=255)

                                                                                                                                     cTGATAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAAtt     >  1:6926443/1‑140 (MQ=255)

                                                                                                                                        aTAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  2:5920161/1‑140 (MQ=255)

                                                                                                                                        aTAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  2:2950086/1‑140 (MQ=255)

                                                                                                                                        aTAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  <  2:1696115/140‑1 (MQ=255)

                                                                                                                                        aTAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  2:1196030/1‑140 (MQ=255)

                                                                                                                                        aTAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  2:8285299/1‑140 (MQ=255)

                                                                                                                                        aTAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  2:1109797/1‑140 (MQ=255)

                                                                                                                                        aTAA‑CAA‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  1:7312884/1‑140 (MQ=255)

GTCCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑A‑AAA‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGT  >  NZ_CP025268/4542752‑4543026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`