Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,505,5590TA27.1% 536.5 / 75.0 294intergenic (+90/+62)phoB/yhfSDNA‑binding transcriptional regulator FrlR/aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base T (58/149);  new base A (74/3);  total (141/153)
Fisher's exact test for biased strand distribution p-value = 1.32e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 311 total aligned reads displayed.

TCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGC  >  NZ_CP025268/3505420‑3505698
                                                                                                                                           |                                                                                                                                            
tctGCCGCGCCAATCGCATCACCTTAACTCTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                              <  2:3890964/140‑1 (MQ=255)
tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                              <  2:4578027/140‑1 (MQ=255)
       cgcCAATCTCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCGAAACCACACCCCa                                                                                                                                       >  1:2653564/1‑140 (MQ=38)
       cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAACCAAAACCACACCCCa                                                                                                                                       >  1:10259006/1‑140 (MQ=39)
       cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCGAACCCACAccccc                                                                                                                                       >  1:9356459/1‑139 (MQ=255)
         ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                     <  1:2236052/140‑1 (MQ=255)
         ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCACACCCGAACCCACACCCCcaa                                                                                                                                     >  1:2317662/1‑140 (MQ=38)
           aaTCGCAACACCTTCACGCTTGATAATAACAGACATTAACCGTACGCCGGATAAGACGCGCCAGCATCGCATCCGGAGATGCTGGCGCGTTGAATTTTACATCCCGAACGTTCCCCTCCCCCTACCCCACTCCCCAAAgg                                                                                                                                   <  2:7833061/140‑1 (MQ=255)
              cGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGc                                                                                                                                <  2:8424005/140‑1 (MQ=255)
                cATCACCTTAACTATTGATAATAAAAGACCTTACCCGGAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                              <  2:9078876/140‑1 (MQ=255)
                   cACCTTCCCTATTGATCATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACCTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                           <  1:7015432/140‑1 (MQ=255)
                     ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGG‑a                                                                                                                        >  2:10254994/1‑140 (MQ=255)
                     ccTTAACTATTGATAATAAAAGACATAAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑a                                                                                                                        <  2:7666378/140‑1 (MQ=255)
                     ccTTAACTATTGAGAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑a                                                                                                                        <  2:8602155/140‑1 (MQ=255)
                     ccTTAACAAATGATAATAAAACACATTAACCTTAGGCAGGATAAGCTGCGCCAGCATCGCATCCGGCGATGCTGTCTCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTCACCCTCTCCCCAAAGGGGCGAGGGG‑a                                                                                                                        <  2:191613/140‑1 (MQ=38)
                       ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCCAAACCAATAACCAAAGGGGCGAGGGG‑Acc                                                                                                                      >  1:859765/1‑140 (MQ=38)
                        tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCCACCAAACCCACACCCCAAAAGGGCGCGGGG‑ACCg                                                                                                                     >  1:8859087/1‑140 (MQ=38)
                        tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCAAACCCAAAACCAAACCCCCAACGGGGGCGGGG‑ACCg                                                                                                                     >  1:515433/1‑140 (MQ=37)
                          aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGtt                                                                                                                   <  2:6739688/140‑1 (MQ=255)
                          aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACTCCCCAAAGGGGCGAGGGG‑ACCGtt                                                                                                                   >  2:9425465/1‑140 (MQ=255)
                          aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCAGCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGtt                                                                                                                   <  2:1550594/140‑1 (MQ=255)
                          aCTATTGAGAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGtt                                                                                                                   <  1:11194159/140‑1 (MQ=255)
                             aTTGATAATAAAATACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACAACCCAAATGGGCGCGGGG‑ACCGTTCAg                                                                                                                >  2:5838675/1‑140 (MQ=38)
                             aTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCCAAACCACAACCCAAAGGGGGCAGGGG‑Gcccgtaac                                                                                                                >  1:7986334/1‑134 (MQ=37)
                             aTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCACCCCCAAAACCACACCCCAAACGGGCGAGGGG‑GCCCGTCAg                                                                                                                >  1:1442265/1‑140 (MQ=37)
                              ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCCCACCCAAAACCACAACCCAAACGGGCGGGGGG‑ACCGtgaagt                                                                                                               >  1:648449/1‑135 (MQ=37)
                              ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCAAACCCAAACCCACTACCCAAAGGGGCGAGGGG‑GCCGTTCAGt                                                                                                               >  2:3259353/1‑140 (MQ=38)
                              ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCACAACCTAACCCACACCCCAAAAGGGCGGGGGG‑Gcccatcggg                                                                                                               >  1:5333017/1‑133 (MQ=37)
                               tGATACAAAAAGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGATGAATTTTACATCCCGTACGTTCCCCTCCCCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTa                                                                                                              <  1:4987764/140‑1 (MQ=38)
                               tGATAATAAAAGACATTAACCTTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGAGCGTTGAATTTTAAATCCCGTAAGGTCACCCAAACCAAAAACACACACCAAAAGGGCGAGGGGCACCGtaaata                                                                                                               >  1:3104932/1‑135 (MQ=19)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCCAAACCAAAACCCCAAAGGGCGGGGGG‑GCCGGTCAGtt                                                                                                              >  1:4309801/1‑139 (MQ=25)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCAAACCCGCACCCCAAAGGGGCGAGGGG‑GCCGTGCAgga                                                                                                              >  1:9069718/1‑138 (MQ=38)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTACCCAAAGGGGCGAGGGG‑ACCGTTCAGTa                                                                                                              >  2:9289321/1‑140 (MQ=255)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCACTCCCCAAAGGGGCGAGGGG‑ACCCGTCAGTa                                                                                                              >  2:5647794/1‑140 (MQ=255)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCCAAACCACAACCCAAAGGGGCGAGGGG‑ACCCTTCAGTa                                                                                                              >  2:7026748/1‑140 (MQ=38)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCTCTACCCAAAGGGGCGAGGGG‑ACCGTTCAGTa                                                                                                              >  1:7603737/1‑140 (MQ=255)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCAAACCCAAACCCAATACCCAAAGGGGCGTGGGG‑ACCGTTCAGtt                                                                                                              >  1:5699081/1‑139 (MQ=38)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATGTTACATCCCGTACGTTCCCCTCACCCGAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGGTCAGTaa                                                                                                             >  2:4363696/1‑139 (MQ=255)
                                    ataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGaa                                                                                                         <  1:8443954/140‑1 (MQ=255)
                                      aaaaGACATTAACCGTAGGCCGGATAAGATTCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGCGGGG‑ACCGTTCAGTCCGGAAtt                                                                                                       >  2:8675213/1‑140 (MQ=255)
                                        aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCACACCCCAAATGGGCGAGGGG‑ACCGATCAGTACGGAATTTa                                                                                                     >  2:2307235/1‑140 (MQ=38)
                                        aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTa                                                                                                     >  1:7352526/1‑140 (MQ=255)
                                              ttACCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  1:3646142/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCCTCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCATCACCCTAACCCTCTCCCCAAAGGGGCGAGTGG‑ACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:4937872/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:734856/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  1:10896027/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:9698994/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGAAGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  1:8143528/140‑1 (MQ=255)
                                               tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCGAACCCTCTCCCCAAAGGGGCGAGGGG‑CCCGTTCAGTACGGAAGTTAGCTTgtc                                                                                              >  2:8227642/1‑138 (MQ=39)
                                               tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATACGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCTCTCCCCCAAGGGGCGATGGG‑ACCGTTCAGTACGGAATTTTGCtttaa                                                                                              >  2:5211689/1‑137 (MQ=39)
                                                aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACg                                                                                             >  2:8039868/1‑140 (MQ=255)
                                                aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACg                                                                                             <  1:714079/140‑1 (MQ=255)
                                                 aCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACgg                                                                                            >  2:11266867/1‑140 (MQ=255)
                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  2:2537492/140‑1 (MQ=255)
                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  1:6861280/140‑1 (MQ=255)
                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  1:7320770/140‑1 (MQ=255)
                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  2:5432116/140‑1 (MQ=255)
                                                   cGTAGGCCGGAGAAGAAGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  1:6661819/140‑1 (MQ=255)
                                                     tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGACTTTTACATCCCGTACGTTCCCCACACCCTAACCCACTCCCCAAAGGGGGGAGGGG‑ACCGTTAAGTACGGAATTTAGCTTGGCGGTGCt                                                                                        >  2:2731553/1‑140 (MQ=39)
                                                       ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAACCAAACCCACACCCCAAAAGGGCGAGGGG‑ACCCTTCAGTACCGAATTTAGCTTGACGGTGCTAt                                                                                      >  1:8560378/1‑140 (MQ=38)
                                                        gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                     <  2:1770485/140‑1 (MQ=255)
                                                        gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                     <  1:5804616/140‑1 (MQ=255)
                                                        gCCGGATAAGAGGCGCCAGCATCGCCTCCGGCGATGCGGGCGCGTTGCATTTTCCCTCCCGTACGGTCCCCTCCCCCGACCCCTCTCCCCCCAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTGGACGGTGCTATc                                                                                     <  2:7426222/140‑1 (MQ=255)
                                                          cgtaTAAGATGCGCCAGCATCGCCTCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                   <  2:4279761/137‑1 (MQ=255)
                                                          cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                   <  2:2145494/140‑1 (MQ=255)
                                                          cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                   <  1:10076075/140‑1 (MQ=255)
                                                            gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGGTCAGTACGGAAGTTATCGTGACGGTGCGATCCAGc                                                                                 >  2:8978348/1‑140 (MQ=38)
                                                            gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGGAGTTTGCTTGTCGGTGCTATCCAGc                                                                                 >  1:9602981/1‑140 (MQ=38)
                                                            gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACACCCCCAAGGGGCGGGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCGATACAGc                                                                                 >  2:3528698/1‑140 (MQ=255)
                                                            gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCAAAACCACAACCCAAAGGGGCGAGGGG‑ACCCGTCAGTACGGAAATTAGATTTACGGTGCTaaacacc                                                                                 >  2:1338712/1‑134 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGGGCCCCCACCCGAAACCTCTCCCCCCAGGGGCGAGGGG‑CCCGGTCAGTACGGAATTTATCATTCAGGGGCGATCCAGCa                                                                                >  2:3101843/1‑140 (MQ=255)
                                                                   tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAAATTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAt                                                                          <  1:4007411/140‑1 (MQ=255)
                                                                    gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAACCCACACCCCAAAAGGGCGAGGGG‑GCCGGTCAGTACCGAAATTATCTTGAAGGTGCATAACAGCATTaaaaa                                                                         >  1:2784852/1‑138 (MQ=255)
                                                                      gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGG‑GCCGGTCAGTACGGAATTTAGCTGGACGGTGCTATACAGCCGTAAATTCt                                                                       >  1:6551065/1‑140 (MQ=38)
                                                                      gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCGCACCCTAACCCTCAACCCAAACGGGCGAGGGG‑TCCGTTCCGGACGGGATTTAGCTTTGCGGTGGTATCCAGCAGTAAATACt                                                                       >  1:10372800/1‑140 (MQ=255)
                                                                       ccAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCACTCACCCAAGGGGCGAGGGG‑TCCGTTCAGTACGGAAATTAGCGTGACGGTGCTATACAGCAGTAAAAACTg                                                                      >  2:5289641/1‑140 (MQ=255)
                                                                          gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGNGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAANTACTGGCa                                                                   <  1:6125855/140‑1 (MQ=255)
                                                                           catcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCaa                                                                  <  1:10567572/140‑1 (MQ=255)
                                                                                    cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                                         <  2:6405070/140‑1 (MQ=255)
                                                                                       cGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                   <  1:10625760/111‑1 (MQ=255)
                                                                                       cGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                   >  2:10625759/1‑111 (MQ=255)
                                                                                       cGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAGACTGGCAAAACTCTCACGCa                                                      >  2:10830698/1‑140 (MQ=255)
                                                                                         aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                    <  1:5896032/140‑1 (MQ=255)
                                                                                         aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                    <  2:668537/140‑1 (MQ=255)
                                                                                             tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATTCTGGCATTACTCTCACGCAAAATcc                                                <  2:3742506/140‑1 (MQ=255)
                                                                                                  cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcagc                                           <  1:11416593/140‑1 (MQ=255)
                                                                                                    ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                         <  1:4535048/140‑1 (MQ=255)
                                                                                                     agAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                        <  1:5034064/139‑1 (MQ=255)
                                                                                                       aaTTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                                      <  1:3042648/140‑1 (MQ=255)
                                                                                                            tACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGTGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCtt                                 <  2:161036/140‑1 (MQ=255)
                                                                                                              cATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCa                               <  2:344805/140‑1 (MQ=255)
                                                                                                                    gTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTg                         <  1:1598255/140‑1 (MQ=255)
                                                                                                                        gTTCCCCTCACCCTCACCCTCGCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt                     <  2:2011290/140‑1 (MQ=255)
                                                                                                                             ccTCCCCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCggg                >  2:6161791/1‑140 (MQ=255)
                                                                                                                                  cccTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt           <  2:4118504/140‑1 (MQ=255)
                                                                                                                                      aaCCCTCTCCCCATAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  1:9863813/1‑140 (MQ=255)
                                                                                                                                      aaCCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  1:3781175/1‑140 (MQ=255)
                                                                                                                                       aCCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCACTACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa      >  2:1042681/1‑140 (MQ=255)
                                                                                                                                         ccTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATc    <  2:6862738/140‑1 (MQ=255)
                                                                                                                                           tctcCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  1:9415061/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGG‑ACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGC  >  NZ_CP025268/3505420‑3505698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑