BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,292,991	0	C	T	60.2%	241.6 / inf	324	noncoding (46/132 nt)	CXP41_RS06835	RtT sRNA
Reads supporting (aligned to +/- strand): ref base C (30/95); new base T (99/90); total (138/186)
Fisher's exact test for biased strand distribution p-value = 6.96e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 328 total aligned reads displayed.

GCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTT  >  NZ_CP025268/1292852‑1293126

gCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGc                                                                                                                                          <  2:8531095/140‑1 (MQ=255)

  aGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCg                                                                                                                                        <  2:5706182/140‑1 (MQ=255)

    aaTATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGaa                                                                                                                                      <  1:1999259/140‑1 (MQ=255)

      tttttGATTGCTGATTCGTTTGAGTATTGCTGTTCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAAcc                                                                                                                                    <  1:10083234/138‑1 (MQ=14)

         ttGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAATCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCttg                                                                                                                                 >  2:10878773/1‑139 (MQ=255)

         ttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAccatg                                                                                                                                 >  1:6791229/1‑137 (MQ=11)

          tGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGATCTCTCGCTTTCGCTCGAATCGAACCTTag                                                                                                                                >  1:10301913/1‑139 (MQ=11)

           gATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCttggt                                                                                                                               >  1:2332297/1‑137 (MQ=255)

            aTTGCGGATTCGTTTGAGAATTCCGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCAGCGGCAACCCTCTCACGCAGTCGCTCGAGACGAAccattgtc                                                                                                                              >  1:8859202/1‑134 (MQ=14)

              ttcGGAGTCGTTTGAGTATTCCGGGTCTTATGACAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                            <  1:6143714/138‑1 (MQ=14)

              tGCTGAGTCGTTTGAGAATTCCTGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                            <  2:3502289/140‑1 (MQ=14)

              tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAAACTTAGTCGa                                                                                                                            >  1:10840166/1‑140 (MQ=255)

              tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                            <  2:9179180/140‑1 (MQ=255)

              tGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCATGAGGCAACGGTCTCTCGCTTTCGCTCGAATCGAAACTTGGTCGa                                                                                                                            >  2:1218456/1‑140 (MQ=11)

               gaggATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGATCGAGCCGAACCTTAGTCGaa                                                                                                                           <  2:6790743/138‑1 (MQ=255)

               gCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGCAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGCGCCGAACCTTAGTCGaa                                                                                                                           <  2:9453288/140‑1 (MQ=14)

               gCGGATTCGCTTGAGATTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGaa                                                                                                                           <  1:7068983/140‑1 (MQ=11)

               gCGGAGTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGaa                                                                                                                           <  1:5607032/140‑1 (MQ=255)

                cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCGCGAGCCGAACCATAGTCGAAg                                                                                                                          >  1:8440795/1‑140 (MQ=255)

                 ggATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGc                                                                                                                         <  1:9598055/140‑1 (MQ=255)

                  gATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCg                                                                                                                                        <  1:4063752/124‑1 (MQ=255)

                  gATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCg                                                                                                                                        >  2:4063752/1‑124 (MQ=255)

                  gATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCt                                                                                                                        <  1:7591452/140‑1 (MQ=255)

                    ttCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTtc                                                                                                                      <  2:6514381/140‑1 (MQ=255)

                    ttCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTtc                                                                                                                      <  1:10760311/140‑1 (MQ=255)

                       gtgtGAGATGTCAGGGGCTTTTGATAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCa                                                                                                                   <  1:6614098/137‑1 (MQ=11)

                        tttGAGGATTCCGGGGCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAt                                                                                                                  <  2:553587/139‑1 (MQ=255)

                        tttGAGCATTCCGGGGCTTTAGACAGTGATGGGGGGGGGGGAAGGATTACTCCGCGCGGCGCGCGTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGCGCCGAACCTTAGTCGACGCTtctcct                                                                                                                  <  2:3798994/139‑3 (MQ=14)

                        tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAt                                                                                                                  <  1:1592705/139‑1 (MQ=255)

                        tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAt                                                                                                                  <  1:10200062/139‑1 (MQ=255)

                        tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAt                                                                                                                  <  2:2420664/139‑1 (MQ=255)

                                 tCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCAACCTTCCCCg                                                                                                         >  2:2067952/1‑140 (MQ=255)

                                   cGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCa                                                                                                       <  1:3289798/140‑1 (MQ=25)

                                       gCTTTAGCAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                   <  2:7335037/140‑1 (MQ=255)

                                         ttttGCAAGTGATGGTGGTGGGGGAAGGATTACTCTGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                 <  1:578473/140‑1 (MQ=12)

                                         ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                 <  1:3629652/140‑1 (MQ=17)

                                                          gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGACAGCCGCAACGCTCTCTCCCAGACGCTCGAGCCGAACCTTACTACAAGCATATCATACTTCCCCGCATGGGCCCGATATTTTATTGCGGa                                                                                >  1:7272616/1‑140 (MQ=11)

                                                                    gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                      <  1:7024673/140‑1 (MQ=255)

                                                                    gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                   <  1:10900660/111‑1 (MQ=17)

                                                                    gATGACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                      <  1:9770393/140‑1 (MQ=11)

                                                                     aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGGCGCTCGATTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTGAAATGCGGAGTCGTGGGGac                                                                     >  2:9098869/1‑139 (MQ=11)

                                                                         cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑c                                                                >  2:10497063/1‑140 (MQ=255)

                                                                             gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAggg                                                            <  2:3995432/140‑1 (MQ=255)

                                                                              cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCCACGCTCTCTCGCTGGCGCTAGAGTCGCACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGCATATTTAATTGCGGATTCGGTGGGAAGTT‑CAGGGa                                                           >  2:4768802/1‑140 (MQ=11)

                                                                              cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGATGCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGGAGAATATTTACTTGCGGAGTCGTTGGGAAGTT‑CAGGGa                                                           >  2:233428/1‑140 (MQ=14)

                                                                              cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGa                                                           >  2:9606501/1‑140 (MQ=255)

                                                                              cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGa                                                           >  2:4676196/1‑140 (MQ=255)

                                                                              cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGa                                                           <  1:8988084/140‑1 (MQ=255)

                                                                              cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCCTCCCCGCAATGGCCGAATATTTAAGTGCGGATTCGTTGGGAAGTT‑GAgggc                                                           >  2:8764766/1‑139 (MQ=14)

                                                                              cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGACTATTTTATTGCGGATTCGTTGGGAAGGT‑CAGGGa                                                           >  2:3279537/1‑140 (MQ=11)

                                                                              cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCAAGGGCAGAAAATTTTATTGAGGATTCGTTGGGAAGTT‑AAGGGa                                                           >  2:1543506/1‑140 (MQ=11)

                                                                                   cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGTAGCTTCTCATCCTTCCCCGCATGGGGCGAATATTTAATTGGGGGGTCGGTGGGGAGGT‑CAggggagttt                                                      >  1:3896032/1‑134 (MQ=11)

                                                                                   cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACtttt                                                      >  2:10643260/1‑140 (MQ=255)

                                                                                   cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACtttt                                                      <  1:8116318/140‑1 (MQ=255)

                                                                                   cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACtttt                                                      <  1:7622945/140‑1 (MQ=255)

                                                                                   cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACtttt                                                      <  2:1352497/140‑1 (MQ=255)

                                                                                   cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCATACCCCCATGGGCCGAATATTTAATTGCGGTGTCGGTGGGAAGTT‑CAGGGTCgttt                                                      >  1:8633734/1‑140 (MQ=11)

                                                                                   cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGACTATTTTATTGCGGATTCGGTGGGGAGTT‑CAGGGACtttt                                                      >  2:7754903/1‑140 (MQ=11)

                                                                                   cgcgcTTCGCCCTTCGGGTCGTTGACTGCGGCCACGCTCTCACGCTGGCGCTCAAGTCGAACCGTGGGCGTAGCTTATCAACCTTACCCGCAAGGGCAGAATATTTAATTGCGGGTTCGTTTGGAAGTTACAGGGaagtc                                                       >  2:5135341/1‑136 (MQ=2)

                                                                                    gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTg                                                     <  2:4440266/140‑1 (MQ=255)

                                                                                      gcTTCGCCCTTCGGGTCGTTGCCAGCGGCACCGCGCGCTCGCTGGCGCTCGAGTCGCACCTTGGTCGAAGCTTCTCATCCTGCCCCGCATGGGCAGAATATTTAAGTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGaa                                                   <  1:9874595/140‑1 (MQ=14)

                                                                                       cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGaaa                                                  <  2:9791257/140‑1 (MQ=255)

                                                                                        ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGATCCTTGGTCGAAGGTTCTCATCCTTCCCCGCGTGGGCAGTATATTTAATTGCGGATTGGGTGGGAAGTT‑GAGGGTGTGTTGaatg                                                 >  1:10376308/1‑138 (MQ=11)

                                                                                        ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAg                                                 <  2:705745/140‑1 (MQ=255)

                                                                                        ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGTCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                 >  2:2739073/1‑93 (MQ=16)

                                                                                           gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGTTATTT‑CAGGGACTTTTGAAAGTGA                                              <  1:504150‑M1/140‑1 (MQ=255)

                                                                                           gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGA                                              <  2:7518742‑M1/140‑1 (MQ=255)

                                                                                           gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGA                                              <  2:5341778‑M1/140‑1 (MQ=255)

                                                                                           gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGA                                              <  1:4621289‑M1/140‑1 (MQ=255)

                                                                                            cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGAT                                             <  1:10947106‑M1/140‑1 (MQ=255)

                                                                                            cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGAT                                             <  1:4771253‑M1/140‑1 (MQ=255)

                                                                                            cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGAT                                             <  1:5381221‑M1/140‑1 (MQ=255)

                                                                                               ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGT                                          <  1:10643261‑M1/140‑1 (MQ=255)

                                                                                                tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTGGGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCCGAATAATGAATTGCGGAGTCGGTGGGCAGTG‑CAGGGACTTTTGCAAGTGAtggtg                                         >  2:5917387/1‑140 (MQ=11)

                                                                                                tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCCCGTCGAACCTTGGTCGAAGCTTCTCAGCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGTG                                         <  1:9463417‑M1/140‑1 (MQ=255)

                                                                                                 cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCCAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGTGG                                        <  2:7690055‑M1/140‑1 (MQ=255)

                                                                                                    gTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACGTTTGAAAGTGATGGTGGTGG                                     >  1:10578835‑M1/1‑140 (MQ=255)

                                                                                                    gTCGTTGCCGGCGGCAACGCTCTCTCGCTGTCGCTCGCGGCGAACCTTAGTCGAAGCTTCTCAAACTTCCCCGCAAGGGCAGAATATTGAATTGCGGATTCGGTTGGTAGTT‑AAGGGACTTTTGAAAGTGAtgggggggg                                     >  1:9136552/1‑140 (MQ=11)

                                                                                                       gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCCAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGTGGTGGGGG                                  <  2:10578629‑M1/140‑1 (MQ=255)

                                                                                                         tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGTGGTGGGGGAA                                <  1:2574306‑M1/140‑1 (MQ=255)

                                                                                                             tGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACATTGGTAGAAACTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCTTTGGTAATTT‑CAGGGACTAGTGAAAGTGATGTGGGTGTGGGAAgggt                            >  2:1919942/1‑138 (MQ=11)

                                                                                                                 gCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGGAGTT‑CAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcga                        <  2:6042581‑M1/140‑4 (MQ=255)

                                                                                                                     cGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTGAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaacct                    >  2:10784204‑M1/1‑133 (MQ=255)

                                                                                                                     cGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTg                                               >  1:1344989/1‑113 (MQ=11)

                                                                                                                          tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGTGGTGGGGGACGGATTcgaaccttcgaa               >  2:3341352‑M1/1‑128 (MQ=255)

                                                                                                                            tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCAGAATATGTCATTGCGGATTCGTTGGGAAGTT‑CAGGGACTGTTGACAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagt             >  2:5176704‑M1/1‑126 (MQ=255)

                                                                                                                                tggcgctCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGAGTCGTGGGGAAGTT‑CAGGGAGTTTTGAAGGTGATGGTGGTGGGGGAAGGATTcgatccttcgaagtcgat         >  2:6756408‑M1/4‑122 (MQ=255)

                                                                                                                                 ggcgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTGGGGAAGTT‑CAGGGACTTTTGAACGTGATGGTGGTGGGGGACGGATTcgaaccttcgaagtcgaag        >  2:11069824‑M1/3‑121 (MQ=255)

                                                                                                                                 ggcgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTGAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGGAAGGGATGGTGGTGGGGGCCGGCTTcgaaccggcgaagtcgctg        >  1:11144305‑M1/3‑121 (MQ=255)

                                                                                                                                  gcgCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGACGTT‑CAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatga       >  1:11302693‑M1/2‑120 (MQ=255)

                                                                                                                                  gcgCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGTGGTGGGGGCAGGATTcgaaccttcgaagtcgatga       >  1:7836950‑M1/2‑120 (MQ=255)

                                                                                                                                  gcgCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatga       >  1:5217315‑M1/2‑120 (MQ=255)

                                                                                                                                  gcgCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatga       >  1:4450210‑M1/2‑120 (MQ=255)

                                                                                                                                   cGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgac      <  2:7928204‑M1/140‑22 (MQ=255)

                                                                                                                                    gCTCGAGTCGAACCTGGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAGTGCGGATTCGTTGGGAAGTT‑CAGGGCCTTTTGAAAGTGAGGGTGGGGGGGGAAGGATTcgaaccttcgaagtcgatgacg     >  2:8314621‑M1/1‑118 (MQ=255)

                                                                                                                                    gCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                                 >  1:378993‑M1/1‑112 (MQ=255)

                                                                                                                                     cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacgg    >  2:7224090‑M1/1‑117 (MQ=255)

                                                                                                                                     cTCGAGTCGAACCTTGGTCGAAGCGTCTCATCCTTCCCCGCCTGGGCAGCATATTTAATTGCGGATTCGTTGGGAATTT‑CAGTGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacgg    <  1:8226427‑M1/140‑24 (MQ=255)

                                                                                                                                     cTCCAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacgg    <  1:11371897‑M1/140‑24 (MQ=255)

                                                                                                                                       cGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggca  <  2:10254840‑M1/140‑26 (MQ=255)

GCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTT‑CAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTT  >  NZ_CP025268/1292852‑1293126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`