BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	671,996	A→C	F613V (TTT→GTT)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	671,996	0	A	C	99.7%	2126.1 / NA	611	F613V (TTT→GTT)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base A (2/0); new base C (303/305); total (306/305)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 612 total aligned reads displayed.

CCATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCACCATA  >  NZ_CP025268/671859‑672132

ccATTCGAGAGTCATATCAGCCGGAGAAGCAGACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCa                                                                                                                                        >  1:6444604/1‑140 (MQ=255)

ccATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCa                                                                                                                                        >  1:3313154/1‑140 (MQ=255)

   ttCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGtt                                                                                                                                     >  1:6972760/1‑140 (MQ=255)

       agagTCATATCAGCCGGAGAAGCAAACATCATAAACAGCCGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATg                                                                                                                                 <  2:8358780/140‑1 (MQ=255)

       agagTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATg                                                                                                                                 >  1:9530368/1‑140 (MQ=255)

          gTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGcc                                                                                                                              >  2:10811111/1‑140 (MQ=255)

           tCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCt                                                                                                                             >  2:1443196/1‑140 (MQ=255)

             atatCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGc                                                                                                                           >  1:1131758/1‑140 (MQ=255)

             atatCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGc                                                                                                                           >  1:10011684/1‑140 (MQ=255)

                    ccGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCt                                                                                                                    >  2:4771166/1‑140 (MQ=255)

                    ccGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCt                                                                                                                    <  2:10340020/140‑1 (MQ=255)

                    ccGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCt                                                                                                                    >  1:9152705/1‑140 (MQ=255)

                        agaAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCg                                                                                                                >  1:4324960/1‑140 (MQ=255)

                        agaAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCg                                                                                                                >  1:3802980/1‑140 (MQ=255)

                            gCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCttt                                                                                                            <  1:7246759/140‑1 (MQ=255)

                             cAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTCTAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTc                                                                                                           >  2:6646719/1‑140 (MQ=255)

                             cAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTc                                                                                                           >  1:7715034/1‑140 (MQ=255)

                             cAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTc                                                                                                           <  1:4078492/140‑1 (MQ=255)

                                  atcatAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGAt                                                                                                      >  2:4939697/1‑140 (MQ=255)

                                  atcatAAACAGACGAAAGGTGTACTCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACATTTGTACTAAGACTTGGACATTTTGCTCATGACGCAATAATACAGTTCAAGGCCTGCCGCATAATACGCTTTCACGAt                                                                                                      >  1:2606745/1‑140 (MQ=255)

                                   tcatAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATa                                                                                                     <  2:51329/140‑1 (MQ=255)

                                    catAAACAGACGAACGGTGTCCGCGCCGTAACGTTCGACCATCACCTGCGGGTCGATACCGTTGTTCGTCGACGTGGACAGTTTGCGCATGCCGGGATAACCCAGTTCATGGCCTGCCGCATCTTTCGCGGTCACGaatc                                                                                                    >  2:9383251/1‑137 (MQ=255)

                                     atAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACg                                                                                                   <  1:5840773/140‑1 (MQ=255)

                                      tAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACgg                                                                                                  >  2:11201720/1‑140 (MQ=255)

                                         aCAGACGAACGGGGTCCGCGCCGTATCGTTCTACCATCACCTGCGGGTCGATACCGTTGTTCTTCGTCTTGGACATTTTGCTCATGCCGGTTTAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCt                                                                                               <  2:11009681/140‑1 (MQ=255)

                                            gACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTtc                                                                                            >  2:388770/1‑140 (MQ=255)

                                                cccGGTGTCCGCGCCGTAACGTTAAACCATCTCCTGCGTGTCGATACCGTTGTTCTTAGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGt                                                                                        <  2:9044332/138‑1 (MQ=255)

                                                caCGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGt                                                                                        <  2:10574191/139‑1 (MQ=255)

                                                  cGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCa                                                                                      <  2:2476339/140‑1 (MQ=255)

                                                     tgtCCGCGCCGTAACGTTCAACCATAAACTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCTTGGCCTGCCGCATCTGTCGCTTTCAGGATACGGCGTTGCTCGTCACGt                                                                                   >  2:8415064/1‑140 (MQ=255)

                                                       tCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTc                                                                                 >  1:6717108/1‑140 (MQ=255)

                                                       tCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTc                                                                                 <  1:753019/140‑1 (MQ=255)

                                                           cgcCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACg                                                                             >  1:11056071/1‑140 (MQ=255)

                                                           cgcCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACg                                                                             >  1:6230605/1‑140 (MQ=255)

                                                           cgcCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACg                                                                             >  1:10658371/1‑140 (MQ=255)

                                                           cgcCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACg                                                                             <  1:7831273/140‑1 (MQ=255)

                                                             ccGTACCGTTCAACCATCCCCTGCGGGTCGAGACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAACCCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGAt                                                                           <  2:11376489/140‑1 (MQ=255)

                                                               gTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTCTAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAg                                                                         >  2:2081473/1‑140 (MQ=255)

                                                               gTAACGTTCAAACATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAg                                                                         >  2:10080792/1‑140 (MQ=255)

                                                                tAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCCTGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGc                                                                        <  2:9692246/140‑1 (MQ=255)

                                                                tAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGc                                                                        <  2:4270507/140‑1 (MQ=255)

                                                                 aaCGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCa                                                                       <  2:3256712/140‑1 (MQ=255)

                                                                      tCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAAc                                                                  >  1:3693520/1‑140 (MQ=255)

                                                                      tCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAAc                                                                  <  1:9420295/140‑1 (MQ=255)

                                                                        aaCCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCg                                                                >  2:6958235/1‑140 (MQ=255)

                                                                           cATCACCGGCGGGTCGATACCGTTGTTCGTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCgggg                                                             <  1:8110626/140‑1 (MQ=255)

                                                                            agcACCTGCGGGTCGAGACCGGTGTTCTTCGACTTGGACATTTTGCTCAGGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGAGACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGa                                                            <  2:7495142/138‑1 (MQ=255)

                                                                            aTCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGa                                                            <  1:2427503/140‑1 (MQ=255)

                                                                              cACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGaaa                                                          <  1:5408936/140‑1 (MQ=255)

                                                                               aCCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAAc                                                         >  1:5682980/1‑140 (MQ=255)

                                                                                ccTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAAcc                                                        <  1:3539738/140‑1 (MQ=255)

                                                                                  tGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGaa                                                           <  1:1394775/135‑1 (MQ=255)

                                                                                  tGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCa                                                      >  2:2846288/1‑140 (MQ=255)

                                                                                    cGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGt                                                    >  2:3578862/1‑140 (MQ=255)

                                                                                    cGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGt                                                    >  2:3463127/1‑140 (MQ=255)

                                                                                    cGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGt                                                    >  1:11460940/1‑140 (MQ=255)

                                                                                        tCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACg                                                >  1:9832008/1‑140 (MQ=255)

                                                                                           ctACCGTTGTTCTTCGACGTGGCCATTTTGCTCATGCCTGTATAAACCAGTTCCTGGCCTGCCGCATCGTTCGCTTTCACGAGACGGCCTTTCTCGTCACGTTCAACGATAGCTTCCACCGGGGAAGCCCAGTGACGTTc                                             >  2:7231282/2‑140 (MQ=255)

                                                                                           aTACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTc                                             >  1:4595435/1‑140 (MQ=255)

                                                                                           aTACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTc                                             >  1:10373191/1‑140 (MQ=255)

                                                                                                 ttgttCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGtt                                       <  1:3949316/140‑1 (MQ=255)

                                                                                                 ttgttCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGtt                                       <  2:1775872/140‑1 (MQ=255)

                                                                                                 ttgttCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGtt                                       >  1:8259986/1‑140 (MQ=255)

                                                                                                      cttcGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGc                                  <  2:8670328/140‑1 (MQ=255)

                                                                                                      cttcGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGc                                  <  2:3696043/140‑1 (MQ=255)

                                                                                                      cttcGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGc                                  <  2:6342200/140‑1 (MQ=255)

                                                                                                      cttcGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGc                                  <  1:5068695/140‑1 (MQ=255)

                                                                                                      cttcGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTGTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGc                                  >  2:7980805/1‑140 (MQ=255)

                                                                                                       ttcGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGcc                                 <  1:7470380/140‑1 (MQ=255)

                                                                                                       ttcGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGcc                                 <  1:1914782/140‑1 (MQ=255)

                                                                                                         cGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCaa                               >  2:5400957/1‑140 (MQ=255)

                                                                                                         cGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCaa                               <  2:65656/140‑1 (MQ=255)

                                                                                                         cGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCaa                               >  1:467092/1‑140 (MQ=255)

                                                                                                           aCTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACa                             <  1:1620038/140‑1 (MQ=255)

                                                                                                           aCTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACa                             <  2:8847604/140‑1 (MQ=255)

                                                                                                              tGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACAtag                          >  1:10927340/1‑140 (MQ=255)

                                                                                                                gACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACAtagta                        >  1:971020/1‑140 (MQ=255)

                                                                                                                 aCATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACAtagtag                       >  2:1647196/1‑140 (MQ=255)

                                                                                                                  cATTGTGCTCATGCCGGAATAAACCACTTCATGGCCTGCCGCATCTTTCGCTTGCACGATACGGCATTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGGTCGCCGTTTTCGCCAACATAGTAGa                      <  2:8215788/140‑1 (MQ=255)

                                                                                                                      ttGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGc                  >  1:5538688/1‑140 (MQ=255)

                                                                                                                       tGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCa                 <  1:6002509/140‑1 (MQ=255)

                                                                                                                       tGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCa                 <  1:8723783/140‑1 (MQ=255)

                                                                                                                       tGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCa                 <  2:1553431/140‑1 (MQ=255)

                                                                                                                        gCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCAt                <  1:8448607/140‑1 (MQ=255)

                                                                                                                            aTGCCGGTATAACCCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGc            <  1:10143076/140‑1 (MQ=255)

                                                                                                                            aTGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGc            <  2:2991641/140‑1 (MQ=255)

                                                                                                                            aTGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGc            <  2:4184651/140‑1 (MQ=255)

                                                                                                                             tGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGcc           >  1:8906070/1‑140 (MQ=255)

                                                                                                                              gCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCa          <  2:1363167/140‑1 (MQ=255)

                                                                                                                              gCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCa          <  2:7902310/140‑1 (MQ=255)

                                                                                                                              gCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCa          <  2:4587983/140‑1 (MQ=255)

                                                                                                                              gCCGGTATAAACCAGTTCATGGCCAGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCACCGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATCGTAGAAGGCATCTGCCa          <  1:4880189/140‑1 (MQ=255)

                                                                                                                                  gTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCAc      <  2:589552/140‑1 (MQ=255)

                                                                                                                                  gTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCAc      <  1:4195539/140‑1 (MQ=255)

                                                                                                                                   tataAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCAcc     >  1:8374696/1‑140 (MQ=255)

                                                                                                                                   tataAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCAcc     <  2:270399/140‑1 (MQ=255)

                                                                                                                                   tataAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCAcc     <  1:2877283/140‑1 (MQ=255)

                                                                                                                                   tataAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCAcc     >  1:214971/1‑140 (MQ=255)

                                                                                                                                    ataAACCATTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCATTCTCGTCACGTTCCACGATAGCATCAACCGGTGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTTGACGGCATCTGCCAGCAcct    <  2:5605561/140‑2 (MQ=255)

                                                                                                                                    ataAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCACCa    <  2:2253056/140‑1 (MQ=255)

CCATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCACCATA  >  NZ_CP025268/671859‑672132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`