BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	801,562	C→A	L54I (CTT→ATT)	ybhJ →	hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	801,562	0	C	A	99.4%	1928.0 / NA	497	L54I (CTT→ATT)	ybhJ	hydratase
Reads supporting (aligned to +/- strand): ref base C (1/2); new base A (200/292); total (202/295)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 503 total aligned reads displayed.

GCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGT  >  NZ_CP025268/801425‑801698

gCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAAtt                                                                                                                                        >  1:769959/1‑140 (MQ=255)

gCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAAtt                                                                                                                                        <  1:9066263/140‑1 (MQ=255)

           gCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCAGAATACGTCCGGAAATATGGATAAAATTACAATTAAGtt                                                                                                                             >  2:866880/1‑140 (MQ=255)

             cAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTg                                                                                                                           <  1:4404366/140‑1 (MQ=255)

              aGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGa                                                                                                                          <  1:10291135/140‑1 (MQ=255)

              aGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGa                                                                                                                          <  1:1906597/140‑1 (MQ=255)

                taataaCGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCGTCGTATAATACGTCCGGAAATAGGGATAAAATTAAAATTAAGTTTGAtt                                                                                                                        >  1:11204035/1‑140 (MQ=255)

                aaataaCGAAATAATTGCCGAAGAACATTTCAACGGCGAAATGAAAAAAGAAGAAGCCAAAAAACGCACAATTGCCTGGTCTATTCTCTCTTCGAATAATACGTCAGGAAATATGGATAAAATTAAAATTAAGTTTGAtt                                                                                                                        >  2:1547910/2‑140 (MQ=255)

                   taaCGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCAt                                                                                                                     >  1:3396607/1‑140 (MQ=255)

                       gAAATAATTGCCGAATAACATTTCACCTGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAATATTATGTTTGATTCATTAGc                                                                                                                 <  2:4835047/140‑1 (MQ=255)

                       gAAATAAGTGCCGAAGAACATTTCCCCGGCGAAATTAAAAAAGAAGAAGCCCACAAAGGCTCTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGc                                                                                                                 <  2:8837019/140‑1 (MQ=255)

                          ataatTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCctc                                                                                                              >  2:7895342/1‑140 (MQ=255)

                          ataatTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTAGTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCctc                                                                                                              >  1:6958123/1‑140 (MQ=255)

                           taatTGCCGAAGAACATTTCACCGGCGAAAATAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGATTGATTCATTAGCctct                                                                                                             <  2:6322949/140‑1 (MQ=255)

                            aatTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAGGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCtctc                                                                                                            <  1:3002328/140‑1 (MQ=255)

                            aatTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCtctc                                                                                                            <  1:5125516/140‑1 (MQ=255)

                             atTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCa                                                                                                           >  2:8208710/1‑140 (MQ=255)

                             atTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAAGATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCa                                                                                                           <  2:9925759/140‑1 (MQ=255)

                                gCCGAAGAACATTTCACCTTCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGa                                                                                                        <  2:4172109/140‑1 (MQ=255)

                                 ccGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGat                                                                                                       <  1:3335674/140‑1 (MQ=255)

                                     agaaCATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTa                                                                                                   <  1:7532611/140‑1 (MQ=255)

                                       aaCATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTAcc                                                                                                 <  2:3790855/140‑1 (MQ=255)

                                       aaCATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTAcc                                                                                                 <  2:11041459/140‑1 (MQ=255)

                                         cATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCtt                                                                                               <  2:4339409/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                            <  1:8948360/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                            <  2:2729868/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                            <  1:56895/140‑1 (MQ=255)

                                              cACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgttg                                                                                          >  2:9677816/1‑140 (MQ=255)

                                              cACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgttg                                                                                          <  2:278679/140‑1 (MQ=255)

                                                 cGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTa                                                                                       <  1:1943623/140‑1 (MQ=255)

                                                 cGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTa                                                                                       <  1:10558032/140‑1 (MQ=255)

                                                 cGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTa                                                                                       <  2:9222701/140‑1 (MQ=255)

                                                 cGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTa                                                                                       <  1:4613452/140‑1 (MQ=255)

                                                 cGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTa                                                                                       <  2:49484/140‑1 (MQ=255)

                                                  ggCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTAt                                                                                      <  2:3496030/140‑1 (MQ=255)

                                                   gCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTAtt                                                                                     >  2:5512870/1‑140 (MQ=255)

                                                    cGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTg                                                                                    >  1:8203941/1‑140 (MQ=255)

                                                    cGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTg                                                                                    >  1:10251992/1‑140 (MQ=255)

                                                    cGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTg                                                                                    >  1:1452896/1‑140 (MQ=255)

                                                      aaaTTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTa                                                                                  >  1:1040237/1‑140 (MQ=255)

                                                        aTTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACa                                                                                <  2:10201113/140‑1 (MQ=255)

                                                        aTTAAAAAACAAGAATCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAATTATGGATAAAATTAATATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACa                                                                                <  2:10895505/140‑1 (MQ=255)

                                                            taatagtagacgCCAAAAAAGGCAGTATTGCCTGGTCTATTCACTCTTCGCATAATTCGTCCGGACATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGAcc                                                                            <  2:5868134/133‑1 (MQ=255)

                                                                 gaagaagCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTaa                                                                       <  2:2023266/140‑1 (MQ=255)

                                                                  aagaagCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTaaa                                                                      <  2:726080/140‑1 (MQ=255)

                                                                  aagaagCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTaaa                                                                      <  1:1197588/140‑1 (MQ=255)

                                                                      agCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGt                                                                  <  1:799424/140‑1 (MQ=255)

                                                                      agCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGt                                                                  <  2:5411743/140‑1 (MQ=255)

                                                                       gCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTc                                                                 <  1:6189014/140‑1 (MQ=255)

                                                                         cAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCg                                                               >  1:2336883/1‑140 (MQ=255)

                                                                           aaaaaGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGt                                                             <  1:2388403/140‑1 (MQ=255)

                                                                               aGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATgg                                                         >  1:8420812/1‑140 (MQ=255)

                                                                                          ccTGGGCTATTCTCTCTTCGCATACTACGTCCGGAAATATGGATAAAATTATAATTAAGTTTGCTACGTTAGCCTATAACGATATTACCTTGGGTGGTGTTGTACAGACCGCTAAAGCGTCCGGAATGGACCGTTTccag                                              >  2:6554558/1‑138 (MQ=255)

                                                                                            tGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCt                                            <  1:8767170/140‑1 (MQ=255)

                                                                                            tGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCt                                            <  2:10510546/140‑1 (MQ=255)

                                                                                            tGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCt                                            <  2:10341633/140‑1 (MQ=255)

                                                                                               tCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGcc                                         >  1:9739748/1‑140 (MQ=255)

                                                                                               tCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGcc                                         <  2:4771922/140‑1 (MQ=255)

                                                                                                 tATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGt                                       <  1:9403894/140‑1 (MQ=255)

                                                                                                 tATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGt                                       >  2:3203383/1‑140 (MQ=255)

                                                                                                      tctctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTAtgtg                                  <  1:10063252/140‑1 (MQ=255)

                                                                                                      tctctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTAtgtg                                  <  1:5369586/140‑1 (MQ=255)

                                                                                                        tctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGata                                                                                                      >  1:7228158/1‑70 (MQ=255)

                                                                                                        tctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCtt                                                                                               <  1:7085769/77‑1 (MQ=255)

                                                                                                        tctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCtt                                                                                               >  2:7085769/1‑77 (MQ=255)

                                                                                                        tctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCt                                >  1:4024706/1‑140 (MQ=255)

                                                                                                        tctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCt                                <  1:9580222/140‑1 (MQ=255)

                                                                                                        tctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGGTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCt                                >  1:6792369/1‑140 (MQ=255)

                                                                                                         ctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTg                               >  2:1337733/1‑140 (MQ=255)

                                                                                                         ctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCGTTGTTGGTATTGTACAGACCGCTATCGCGTCCGGTATGGAACGGTTCCCGCTGCCGTTTGTGcgg                               >  2:4069130/1‑138 (MQ=255)

                                                                                                           tCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCtctc                                                                                                            <  2:1911223/61‑1 (MQ=255)

                                                                                                           tCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGAc                             >  1:4735349/1‑140 (MQ=255)

                                                                                                           tCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGAc                             >  2:9055930/1‑140 (MQ=255)

                                                                                                            cGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGAcc                            <  1:10802033/140‑1 (MQ=255)

                                                                                                             gCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCa                           >  2:5725128/1‑140 (MQ=255)

                                                                                                             gCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCa                           >  1:6533124/1‑140 (MQ=255)

                                                                                                             gCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCa                           >  1:3897655/1‑140 (MQ=255)

                                                                                                               ataataCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAAc                         <  2:8910061/140‑1 (MQ=255)

                                                                                                               ataataCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAAc                         <  1:1688754/140‑1 (MQ=255)

                                                                                                                 aataCGTCCGGAAATAGGGTTAAATTGAAAATTAAGTTTGTTTCATTTGCCTCTCACGATATTACCTTTGTTGGGATTGTACAGAACGATAAAGCGTCCGGTATGGAACTTTTCCCGCTGCCGTATGTGCTGACCAACTg                       <  1:9171774/140‑1 (MQ=255)

                                                                                                                  ctccGTCCGGAAATATGGATAAAATTAAAAGTAAGTTTGATTCAGTTGCCTCGCACGATTTTACGTTTGTGGGTAGTGTACAGACCGCTAAAGCGTGCGGTTTGGAACGTGTCCCGCGGCCGTAGGTGCTGTCCAACTGc                      >  2:1205243/4‑140 (MQ=255)

                                                                                                                  ataCGTCCGGACATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGGTGGGATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGc                      >  2:2500750/1‑140 (MQ=255)

                                                                                                                  ataCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGc                      >  2:11310276/1‑140 (MQ=255)

                                                                                                                  ataCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGc                      >  1:6596428/1‑140 (MQ=255)

                                                                                                                  ataCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGc                      >  1:6014997/1‑140 (MQ=255)

                                                                                                                   taCGTCCGGTAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGcc                     <  2:2249959/140‑1 (MQ=255)

                                                                                                                   taCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGcc                     <  2:7709777/140‑1 (MQ=255)

                                                                                                                   taCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGcc                     <  2:10698184/140‑1 (MQ=255)

                                                                                                                   taCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGcc                     <  1:3180711/140‑1 (MQ=255)

                                                                                                                      gTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATa                  >  2:3390363/1‑140 (MQ=255)

                                                                                                                        ccGGACATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAATGCGTCGGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGACATAac                >  2:6803288/1‑140 (MQ=255)

                                                                                                                        ccGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGcc                     >  1:4913875/1‑135 (MQ=255)

                                                                                                                        ccGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAac                >  1:3627826/1‑140 (MQ=255)

                                                                                                                        ccGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAac                >  1:11390115/1‑140 (MQ=255)

                                                                                                                        ccGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAac                >  1:4488168/1‑140 (MQ=255)

                                                                                                                        ccGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCATATGTGCTGACCAACTGCCATAac                >  2:6394474/1‑140 (MQ=255)

                                                                                                                           gAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCa                                                                                                           >  1:269981/1‑46 (MQ=255)

                                                                                                                                 tGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTgc       >  1:11028855/1‑140 (MQ=255)

                                                                                                                                    aTAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTa                                                                                  >  2:6955681/1‑62 (MQ=255)

                                                                                                                                    aTAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGcc    >  2:4020754/1‑140 (MQ=255)

GCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGT  >  NZ_CP025268/801425‑801698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`